1MTY,1MHZ,1FYZ,1FZ8,1MMO,3DHG,2INP


Conserved Protein Domain Family
AAMH_A

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cd01057: AAMH_A 
Click on image for an interactive view with Cn3D
Aromatic and Alkene Monooxygenase Hydroxylase, subunit A, ferritin-like diiron-binding domain
Aromatic and Alkene Monooxygenase Hydroxylases, subunit A (AAMH_A). Subunit A of the soluble hydroxylase of multicomponent, aromatic and alkene monooxygenases are members of a superfamily of ferritin-like iron-storage proteins. AAMH exists as a hexamer (an alpha2-beta2-gamma2 homodimer) with each alpha-subunit housing one nonheme diiron center embedded in a four-helix bundle. The N-terminal domain of the alpha- and noncatalytic beta-subunits possess nearly identical folds, however, the beta-subunit lacks critical diiron ligands and a C-terminal domain found in the alpha-subunit. Methane monooxygenase is a multicomponent enzyme found in methanotrophic bacteria that catalyzes the hydroxylation of methane and higher alkenes (as large as octane). Phenol monooxygenase, found in a diverse group of bacteria, catalyses the hydroxylation of phenol, chloro- and methyl-phenol and naphthol. Both enzyme systems consist of three components: the hydroxylase, a coupling protein and a reductase. In the MMO hydroxylase, dioxygen and substrate interact with the diiron center in a hydrophobic cavity at the active site. The reductase component and protein coupling factor provide electrons from NADH for reducing the oxidized binuclear iron-oxo cluster to its reduced form. Reaction with dioxygen produces a peroxy-bridged complex and dehydration leads to the formation of complex Q, which is thought to be the oxygenating species that carries out the insertion of an oxygen atom into a C-H bond of the substrate. The toluene monooxygenase systems, toluene 2-, 3-, and 4-monooxygenase, are similar to MMO but with an additional component, a Rieske-type ferredoxin. The alkene monooxygenase from Xanthobacter strain Py2 is closely related to aromatic monooxygenases and catalyzes aromatic monohydroxylation of benzene, toluene, and phenol. Alkane omega-hydroxylase (AlkB) and xylene monooxygenase are members of a distinct class of integral membrane diiron proteins and are not included in this CD.
Statistics
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PSSM-Id: 153115
View PSSM: cd01057
Aligned: 28 rows
Threshold Bit Score: 581.177
Threshold Setting Gi: 10443292
Created: 24-Sep-2002
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 6 residues -Click on image for an interactive view with Cn3D
Feature 1:diiron center [ion binding site]
Evidence:
  • Structure:1MHZ; Methylosinus trichosporium Methane Monooxygenase Hydroxylase (MMOH), a bis-mu-hydroxo-bridged "diamond core" diiron center; putative high-valent intermediate, complex Q.
    View structure with Cn3D
  • Citation:PMID 9070438
  • Structure:1MMO; Methylococcus capsulatus MMOH active site cavity, iron ligands, and exogenous acetate.
    View structure with Cn3D
  • Comment:Exogenous acetate ligand might demarcate where the oxidized substrate normally binds before protonation and release from the active site.
  • Citation:PMID 8255292
  • Structure:1MTY; Methylococcus capsulatus MMOH Fe(III) oxidized form.
    View structure with Cn3D
  • Comment:The two iron atoms are octahedrally coordinated by 2 histidine and 4 glutamic acid residues, a bridging hydroxide ion, and a terminal water molecule.
  • Citation:PMID 9329079
  • Structure:1FYZ; Methylococcus capsulatus MMOH Fe(II) reduced form; carboxylate-bridged diiron center.
    View structure with Cn3D
  • Structure:1FZ8; Methylococcus capsulatus MMOH cocrystallized with dibromomethane: cavities 2 and 3 - putative path to the active site cavity.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                     
1MTY_D     18 LQSFNWDFKNNRt---KYATKYKMan-etKEQFKLIAKEYARMEAVKDERQFGSLQVaLTRLNAGVRVHPKWNETMKVVS 93  Methylococcus caps...
1MHZ_D     27 LQSFNWDFKENRt---KYPTKYHMan-etKEQFKVIAKEYARMEAAKDERQFGTLLDgLTRLGAGNKVHPRWGETMKVIS 102 Methylosinus trich...
AAM19727   29 LQDFTWDFKGKTa---KYPTKYEMdv-ntREQFKLTAKEYARMESIKEERQYGTLLDgLDRLDAGNKVHPKWGEVMKLVS 104 Pseudomonas butano...
AAO48576   13 SKRYDWGFDYARpd-pKFPTRYIIpp-kgKDPFRSMLRGYAAMETEKDNRVYGGLDS-NVRYRNATSAEPRFIEGMKFGI 89  Mycobacterium rhod...
BAF34294   11 VKDFDWEHSYAEkp-dRYPTKYVMpr-ktKDPFRHLIRDYVSMEQEKDDRQYGAMEDaLARSNSAGKAQPRWMEILKIAL 88  Mycobacterium sp. ...
NP_770317  36 IADLGWNPSYVQea-mTFPTDYKItk-apKDPMKQVLRSYFPMQEEKDNRVYGALDA-ALRGDMFRNVEPRWVEWMKLFL 112 Bradyrhizobium jap...
BAD03956   13 ITELSWEPTFATpa-tRFGTDYTFekapkKDPLKQIMRSYFPMEEEKDNRVYGAMDG-AIRGNMFRQVQERWLEWQKLFL 90  Gordonia sp. TY-5
YP_999132  25 IADLGWTPSYVQea-mTFPTDYKIsk-apRDPMKQVLRSYFPMQEEKDNRVYGALDA-ALRGDMFRNVEPRWIEWMKLFL 101 Verminephrobacter ...
CAC10506   20 IGELGWDRTYYPhergKYPSRYKLpnkpgRDPMKQIMGDYLHMQNEKDDRVHGGLDA-AVRAEVPGKAPLRWLELLKPYL 98  Pseudonocardia sp. K1
BAA07114   12 SKSYDWDFTSVErr-pKFETKYKMpk-kgKDPFRVLIRDYMKMEAEKDDRTHGFLDG-AVRTREATRIEPRFAEAMKIMV 88  Gordonia rubripert...
Feature 1           #                             #  #                                        
1MTY_D     94 NFLEVGEYNAIAATGMLWDSAQAAEQKNGYLAQVLDEIRHTHQCAYVNYYFAKngqdpaGHNDARRTrtiGPLWKGMKRV 173 Methylococcus caps...
1MHZ_D    103 NFLEVGEYNAIAASAMLWDSATAAEQKNGYLAQVLDEIRHTHQCAFINHYYSKhyhdpaGHNDARRTraiGPLWKGMKRV 182 Methylosinus trich...
AAM19727  105 NFLETGEYGAIAGSALLWDTAQSPEQRNGYLAQVIDEIRHVNQTAYVNYYYGKhyydpaGHTNMRQLraiNPLYPGVKRA 184 Pseudomonas butano...
AAO48576   90 PSFTDAEYQATCGSGFLIASMKNQELRQGYAGQMLDEVRHTQIEVALRKYYLKnyhdpaGFDIGQIGl-gNHPIGTLARA 168 Mycobacterium rhod...
BAF34294   89 PVVNFGEYAAMKCCGQLVDTVNNAELRQGYMAQMIDEVRHTNQELYLNRYFAKhaadpeGFHIGMKAr-aNNLFGVAGRA 167 Mycobacterium sp. ...
NP_770317 113 AIIPFPEISAARSMAMVGRLAPGEDLRTGFTMQMVDEFRHSTIQMNLKKWYMEnyidpaGFDITEEAf-gKCYATTIGRQ 191 Bradyrhizobium jap...
BAD03956   91 SIIPFPEISAARAMPMAIDAVPNPEIHNGLAVQMIDEVRHSTIQMNLKKLYMNnyidpaGFDITEKAf-aNNYAGTIGRQ 169 Gordonia sp. TY-5
YP_999132 102 AIIPFPEISAARSMAMLGRLAPGEELRTGFTMQMVDEFRHSTIQMNLKKWYMEnyidpaGFDITEAAf-gKCYATTIGRQ 180 Verminephrobacter ...
CAC10506   99 LTVISAEAAATRCMGMLVDALDDPELQNAYYIQQLDEQRHTAMQMNLYRWYMKnmpepvGWNLGLQAvggDSILVAAQNL 178 Pseudonocardia sp. K1
BAA07114   89 PQLTNAEYQAVAGCGMIISAVENQELRQGYAAQMLDEVRHAQLEMTLRNYYAKhwcdpsGFDIGQRGl-yQHPAGLVSIG 167 Gordonia rubripert...
Feature 1                           #                                 #  #                    
1MTY_D    174 FSDGFisGDAV-ECSLNLQLVGEACFTNPLIVAVTEWAAANGDEITPTVFLSIETDELRHMANGYQTVVSIAndpaSAKY 252 Methylococcus caps...
1MHZ_D    183 FADGFisGDAV-ECSVNLQLVGEACFTNPLIVAVTEWASANGDEITPTVFLSVETDELRHMANGYQTVVSIAndpaSAKF 261 Methylosinus trich...
AAM19727  185 FGEGFlaGDAV-ESSINLQLVGEACFTNPLIVSLTEWAAANGDEITPTVFLSIETDELRHMANGYQTIVSIMnnpeTMKY 263 Pseudomonas butano...
AAO48576  169 SFQSFntGDPV-EVSMCLNIVLETAYTNPLVVALPQVAAVNGEHAMPTAFLSIQSDESRHMANGYGTLMSVIqehdNLPF 247 Mycobacterium rhod...
BAF34294  168 ALETFfvGDPV-EGALNLQVVAETAYTNPIFVTLTEVAAANGDNVTPSVFLSVQSDEARHMANGYSTLAAVVsnedNLKY 246 Mycobacterium sp. ...
NP_770317 192 FGEGFitGDAItSANVYLTVVAETAFTNTLFVAMPSEAARNGDYALPTVFLSVQSDESRHIGNGHSMLMSMLkepeNHLL 271 Bradyrhizobium jap...
BAD03956  170 FGEGFitGDAItAANIYLTVVAETAFTNTLFVAMPDEAAANGDYLLPTVFHSVQSDESRHISNGYSILLMALaderNRPL 249 Gordonia sp. TY-5
YP_999132 181 FAEGFltGDAVtAANVFLQVVAETAFTNTLFVAMPSEAARNGDYALPTVFLSVQSDESRHIGNGHSLVMSIIndpdNHLL 260 Verminephrobacter ...
CAC10506  179 TGSFMt-GDPF-QAAVALQVVVETAFTNTILVAFPDVAVRNHDFALPTVMNSVQSDEARHINNGYATLLYLLqepeNAPL 256 Pseudonocardia sp. K1
BAA07114  168 EFQHFntGDPL-DVIIDLNIVAETAFTNILLVATPQVAVANGDNAMASVFLSIQSDEARHMANGYGSVMALLenedNLPL 246 Gordonia rubripert...
Feature 1                                                                                     
1MTY_D    253 LNTDLNNAFWTQQKYFTpVLGMLFEYGSK---FKVEPWVKTWDRWVYEDWggIWIGRLgkyGVES-PRSLKDAKQDA--Y 326 Methylococcus caps...
1MHZ_D    262 LNTDLNNAFWTQQKYFTpVLGYLFEYGSK---FKVEPWVKTWNRWVSEDWggIWIGRLgkyGVES-PRSLRDAKRDA--Y 335 Methylosinus trich...
AAM19727  264 LQTDLDNAFWTQHKFLTpFVGVALEYGSK---YKVEPWAKSWNRWVYEDWagIWLGRLqqfGVKT-PKCLPDAKKDA--V 337 Pseudomonas butano...
AAO48576  248 LQESLDRHFWHQHQSMDtLVGVLSEYFAV---ERPWAYKDVWEEWVVDDFvgSYMSRLspfGLKP-PARLGDVARYV--N 321 Mycobacterium rhod...
BAF34294  247 LQADFDRAFWRQHSFLDpFLGAVYDYFQK---ERGHSYLEKWTEWIEEDWvgSYISKMepyGLAV-PDCFHVAKEQM--R 320 Mycobacterium sp. ...
NP_770317 272 LERDMRYAFWQNHAIVDaAIGTFIEYGTTnrdKNKESYAEMWHRWIFEDYyrTYMLPLekyGIKIhHDDVQAAWKRL--T 349 Bradyrhizobium jap...
BAD03956  250 LERDLRYAWWNNHCVVDaAIGTFIEYGTKdrrKDRESYAEMWRRWIYDDYyrSYLLPLekyGLTIpHDLVEEAWNRIvdK 329 Gordonia sp. TY-5
YP_999132 261 LERDLRYAFWQNHAIVDaAIGTFIEYGTVdrdKNKESYAELWHRWIYEDYyrTYMLPLekyGIKIhHDDIVAAWDRIvkQ 340 Verminephrobacter ...
CAC10506  257 LEQDIQQMFWTVHAFVDaFMGILVEYAPTd-aTDPESWTTKWDRWVNDDYyrSYIVNLgklGLKI-PDSIFKRARERiaA 334 Pseudonocardia sp. K1
BAA07114  247 LNQSLDRHFWRAHKALDnAVGWCSEYGAR---KRPWSYKAQWEEWVVDDFvgGYIDRLsefGVQA-PACLGAAADEV--K 320 Gordonia rubripert...
Feature 1                                                                                     
1MTY_D    327 WAHHDLYLLAYALWPt--GFFRLALPDQEEMEWFEANYPGWYDHYGKIYEEWRARgcedpssgfiplmwfiennhpIYID 404 Methylococcus caps...
1MHZ_D    336 WAHHDLALAAYAMWPl--GFARLALPDEEDQAWFEANYPGWADHYGKIFNEWKKLgyedpksgfipyqwllanghdVYID 413 Methylosinus trich...
AAM19727  338 WAHHDLALLALALWPl--TGIRMELPDSLAMEWFEANYPGWYNHYGKIYEEWRAAgfedpksgfcgalwlmerghgIFVD 415 Pseudomonas butano...
AAO48576  322 DMHHSVAIALAAMWPl--NFWRTDPMGPADYEWFENHYPGWTKSYGGLWDAFRDMsdpssari-----llqelpslPPFC 394 Mycobacterium rhod...
BAF34294  321 WKHHTAAMLAAASWPl--HFWRWDPLTESDFEWFENKYPGWYEHYGPFWENFRQItkaedgvn-----pmaafealPPLC 393 Mycobacterium sp. ...
NP_770317 350 EKHYVHKVAQFFAVGwsaNFWRIEAQTEKDFEWFEHKYPGWYAQFGEFWKWYEKLshrgqtnil---fnsdvgyvyPHRC 426 Bradyrhizobium jap...
BAD03956  330 HYVHEVARFFATGWPv--NYWRIDAMTDTDFEWFEEKYPGWYNKFGKWWENYNRLaypgknkpi---afedvdyeyPHRC 404 Gordonia sp. TY-5
YP_999132 341 NYVHKVAQFFAVGWPv--NFWRIEAQTEKDFEWFEHKYPGWYAEFGDFWKWYAKKsvpgqtnml---fdqecgyvyPHRC 415 Verminephrobacter ...
CAC10506  335 DYHHKVAVGVWASWPf--HYYKYGNLEQKDYDWFESKYPGWNEKFGAFWRGYADVrypgsgplq----lpgllegaGPIC 408 Pseudonocardia sp. K1
BAA07114  321 WSHHTLGQVLSAVWPl--NFWRSDAMGPADFEWFENHYPGWSAAYQGYWEGYKALadpaggri-----mlqelpglPPMC 393 Gordonia rubripert...
Feature 1                                                                                     
1MTY_D    405 RVSQVPfcpslakgaSTLRVHEYNGEMHTFSDQWGERMWLA-EPERY-ECQNIFEQYeg-----------rELSEVIAEL 471 Methylococcus caps...
1MHZ_D    414 RVSQVPfipslakgtGSLRVHEFNGKKHSLTDDWGERQWLI-EPERY-ECHNVFEQYeg-----------rELSEVIAEG 480 Methylosinus trich...
AAM19727  416 HASGLPfcpslakssIKPRFTEYNGKRYAFAEPYGERQWLL-EPERY-EFQNFFEQFeg-----------wELSDLVKAA 482 Pseudomonas butano...
AAO48576  395 QVCHVPcvmp-tlhaPETRIVYGEGKKFAVCSEGCEWIFNL-NPTIYsGCANWWERFdg-----------mDLADVILAL 461 Mycobacterium rhod...
BAF34294  394 QVCQMPcifp-rldcSEVRFADYGGRTVPFCGPMCETLFFQ-EPIRYaQSRNFWQHHdg-----------vELADHLVEN 460 Mycobacterium sp. ...
NP_770317 427 WSCLVPcl-----irEDIVTDEIDGKLHTFAHELDRWTAVEaFAGEYqGRPTPAMGRfsgrrewesvyhnvDIADAIKDL 501 Bradyrhizobium jap...
BAD03956  405 WTCMVPcl-----irEDMVTDKVDGQWRTYCSETCAWTDKVaFRPEYeGRPTPNMGRltgfrewetlhhgkDLADIITDL 479 Gordonia sp. TY-5
YP_999132 416 WSCLVPcl-----irEDFVVDEVDGQIYTYCSEMCHWTHKVaFASEYeGRPTPAMGRfsgrreweecyhgwDLADCIKDL 490 Verminephrobacter ...
CAC10506  409 WTCQLGcl-----rpEEQCHRIVDEHTRFYCSPECKWIDMT-NPGRYvGDRVWFDRYhg-----------wEYSEIVRDL 471 Pseudonocardia sp. K1
BAA07114  394 QVCQVPcvmp-rldmNAARIIEFEGQKIALCSEPCQRIFTN-WPEAYrHRKQYWARYhg-----------wDLADVIVDL 460 Gordonia rubripert...
Feature 1                   
1MTY_D    472 hglrsdgktliAQP 485 Methylococcus capsulatus
1MHZ_D    481 hgvrsdgktliAQP 494 Methylosinus trichosporium
AAM19727  483 ggvrsdgktliAQP 496 Pseudomonas butanovora
AAO48576  462 gyvrpdgktliGQP 475 Mycobacterium rhodesiae
BAF34294  461 gllrsdgktliAQP 474 Mycobacterium sp. TY-6
NP_770317 502 gfvrtdgktlvAQP 515 Bradyrhizobium japonicum USDA 110
BAD03956  480 gyvrddgktliPQP 493 Gordonia sp. TY-5
YP_999132 491 gfvrsdgktlvPQP 504 Verminephrobacter eiseniae EF01-2
CAC10506  472 gflrpdgktltGQP 485 Pseudonocardia sp. K1
BAA07114  461 gyirpdgktliGQP 474 Gordonia rubripertinctus

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