Conserved Protein Domain Family
AOX

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cd01053: AOX 
Alternative oxidase, ferritin-like diiron-binding domain
Alternative oxidase (AOX) is a mitochondrial ubiquinol oxidase found in plants and some fungi and protists. AOX is a member of the ferritin-like diiron-carboxylate superfamily. The plant mitochondrial protein alternative oxidase catalyses dioxygen dependent ubiquinol oxidation to yield ubiquinone and water. AOX is a cyanide-resistant, salicylhydroxamic acid-sensitive oxidase that transfers electrons from ubiquinol to oxygen, bypassing the cytochrome chain. AOX has been proposed to contain a hydroxo-bridged diiron center within a four-helix bundle and a proximal redox-active tyrosine residue. AOX is proposed to be peripherally associated with the matrix side of the inner mitochondrial membrane. Fungal and protozoan AOXs generally exist as monomers. In plants, AOX is dimeric. Pyruvate is an allosteric activator of plant AOX involved in the reversible inactivation of the enzyme though the formation of an intermolecular disulfide bridge between monomeric subunits. The enzyme is non-proton-motive and does not contribute to the conservation of energy. The heat that dissipates from AOX activity is used in thermogenic plants to volatilize primary amines to attract pollinating insects. Other functions have been proposed: i) that the alternative oxidase allows Krebs-cycle turnover when the energy charge of the cell is high, and ii) that the enzyme protects against oxidative stress. The expression of AOX is induced when plants are exposed to a variety of stresses including chilling, pathogen attack, senescence and fruit ripening.
Statistics
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PSSM-Id: 153112
View PSSM: cd01053
Aligned: 31 rows
Threshold Bit Score: 245.58
Threshold Setting Gi: 116055422
Created: 24-Sep-2002
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
diiron binding
Feature 1:diiron binding motif [ion binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                    #                                       #  #                        
P22185       166 FQRRYACRAMMLETVAAVPGMVGGVLLHLKSLRRFEHSg-gWIRALLEEAENERMHLMTFMEVAQPRWYERALVLAVQGV 244 voodoo lily
NP_486136     17 YRDRPYPRFYVLETVARVPYFSYLSVLHLYETLGWWRKa-dWLKVHFAESWNELHHLLIMESLGGAGFWGDRFLAKTAAL 95  Nostoc sp. PCC ...
AAP79178      69 FDGRPLERFWFLETVARMPYLSYVTMLHLYESFGWWRRaaaVKRVHFAEEWNEFHHLLTFEALGGDRSWATRFLAQHAAI 148 Bigelowiella na...
AAM12876     266 YENRPIQRFWFLETVARMPYFSYISMLHLYESLGWWRAgaeLRKIHFAEEWNELHHLQIMESLGGDQLWFDRFAAQHAAI 345 Chlamydomonas r...
XP_001774094  32 YKNRDYARFYVLETIARVPYFAFVSVLHMYESFGWWRRs-dYIKVHFAESWNELHHLLTMEALGGDERWVDRFLAQHIAV 110 Physcomitrella ...
XP_001777033   6 FNDRPIQRFWFLETVARMPYFSYISMLHLYETLGWWRIgaeVRKVHFAEEWNEMHHLRIMESLGGDLEWGDRFFAQHAAF 85  Physcomitrella ...
CAL55767      17 YAGRSYARFHALETVARVPYFSFMSVLHLYETFGWWRRa-dYLKVHFAETMNEYHHLLIMESLGGAELWRDRFFAQHVAV 95  Ostreococcus tauri
XP_002182610 119 TMDRAFARFYALETIARMPYFSYLSVLHLWETLGMWRRa-eYLQVHFAESWNELHHLLIMEELGGNGRWGDRFVAQHIAF 197 Phaeodactylum t...
XP_002293901  92 GEDRAYARFYALETIARVPYFSYLSVLHLYETLGKWRRv-kYLKLHFAESWNEMHHLLIMEELGGSERFFDRFLAQHCAF 170 Thalassiosira p...
CAL58090     247 DETKPVQRFWFLETVARMPYYSYTAALTFYEILGWYRGgaeLRKIHFAEEWNEYHHLLIMESLGGDVSWRDRFLGQHAAL 326 Ostreococcus tauri
Feature 1                                #                                                       
P22185       245 -FFNAYFLGYLLSPKFAHRVVGYLEEEAIHSYTEFLKDIDSGaiqdcPAPAIALDYWRlp-------------------- 303 voodoo lily
NP_486136     96 iYYWIIIAVYFVSPHSAYNFMEQVEQHAYSSYDKFLTTHEAElk-tqPAPEVAKTYYRdgdlymfdefqt--------ah 166 Nostoc sp. PCC ...
AAP79178     149 vYYWVLVLMWLLSPTLAYNFSELIEAHAVDTYGEFADANEELmk-elPAPGIAIQYWMggdmylydefqt-------err 220 Bigelowiella na...
AAM12876     346 lYYWILLGLYVFSPRLAYNFSELIEYHAVDTYGEFWDANEELlk-slPPPLVAAVYYRsqdlymfdsfqt-------sqp 417 Chlamydomonas r...
XP_001774094 111 gYYFMTVVMYLLSPRMAYHFSECVEKHAFHTYDEFIKLHGEElk-klPAPEVAVKYYTsgdlylfdefqt--------dr 181 Physcomitrella ...
XP_001777033  86 fYYWVLNLMFLISPKVAYNFSELIEMHAVDTYGEFADANEELlk-tlPPPPAALEYYEsedlymydefqt--------sq 156 Physcomitrella ...
CAL55767      96 aYYWICVAQYLVSPRWAYNLLEQVESHAYATYDKFLEANEEVlk-ktPAPRVAVAYYTkgdlylfdefq---------tg 165 Ostreococcus tauri
XP_002182610 198 fYYWIVVTLYAVNPTMAYNLNQAVEEEAYETYDGFLQTHAEYlq-sqPAPQAAIRYYTgddlylfhamhwdtrqpkepes 276 Phaeodactylum t...
XP_002293901 171 gYFLIVITLYLINPVQAYNLNQDVEEHAFATYDTFLKENAEMlk-tkPAPKVAIEYYRhgdmymfdefq----------t 239 Thalassiosira p...
CAL58090     327 vYYGVLILLWFMSPALAYNFSELIEAHAVDTYAQFVDQNAELlk-tmPAPRIAVEYYEgadlylfdefqt--------ar 397 Ostreococcus tauri
Feature 1                              #  #           
P22185       304 -------qgSTLRDVVTVVRADEAHHRDVNHFASDVH 333 voodoo lily
NP_486136    167 spserrpniDNLYDVFVAIRDDEMEHVKTMVACQQTN 203 Nostoc sp. PCC 7120
AAP79178     221 lgderrpniTNLYDVICAIRDDEAEHVATMAACQKKG 257 Bigelowiella natans
AAM12876     418 mqnprrpscKTLYDVFKNICDDEMEHVKTMKACQDET 454 Chlamydomonas reinhardtii
XP_001774094 182 tpntrrpkiDNLYDVFYNIREDEAEHCKTMLACQTRG 218 Physcomitrella patens subsp. patens
XP_001777033 157 apetrrpkiNNLYDVFKAISGDELEHVKTMSACQTLD 193 Physcomitrella patens subsp. patens
CAL55767     166 ekgarrpkiDNLYDVFVNVRNDEAEHMKTMEFCQRPG 202 Ostreococcus tauri
XP_002182610 277 pveqrrptcETLYDTIRNIRDDELEHVKTMACLQDES 313 Phaeodactylum tricornutum CCAP 1055/1
XP_002293901 240 ecelrrpeiNNLYDVFVAIRDDEMAHVKTMEKLQTEL 276 Thalassiosira pseudonana CCMP1335
CAL58090     398 evrtrrpriRTLYDVFSNIRDDEGEHVSTMNACQKED 434 Ostreococcus tauri

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