1OQ9,1AFR,2UW1


Conserved Protein Domain Family
Acyl_ACP_Desat

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cd01050: Acyl_ACP_Desat 
Click on image for an interactive view with Cn3D
Acyl ACP desaturase, ferritin-like diiron-binding domain
Acyl-Acyl Carrier Protein Desaturase (Acyl_ACP_Desat) is a mu-oxo-bridged diiron-carboxylate enzyme, which belongs to a broad superfamily of ferritin-like proteins and catalyzes the NADPH and O2-dependent formation of a cis-double bond in acyl-ACPs. Acyl-ACP desaturases are found in higher plants and a few bacterial species (Mycobacterium tuberculosis, M. leprae, M. avium and Streptomyces avermitilis, S. coelicolor). In plants, Acyl-ACP desaturase is a plastid-localized, covalently ACP linked, soluble desaturase that introduces the first double bound into saturated fatty acids, resulting in the corresponding monounsaturated fatty acid. Members of this class of soluble desaturases are specific for a particular substrate chain length and introduce the double bond between specific carbon atoms. For example, delta 9 stearoyl-ACP is specific for stearic acid and introduces a double bond between carbon 9 and 10 to yield oleic acid in the ACP-bound form. The enzymatic reaction requires molecular oxygen, NAD(P)H, NAD(P)H ferredoxin oxido-reductase and ferredoxin. The enzyme is active in the homodimeric form; the monomer consists mainly of alpha-helices with the catalytic diiron center buried within a four-helix bundle. Integral membrane fatty acid desaturases that introduce double bonds into fatty acid chains, acyl-CoA desaturases of animals, yeasts, and fungi, and acyl-lipid desaturases of cyanobacteria and higher plants, are distinct from soluble acyl-ACP desaturases, lack diiron centers, and are not included in this CD.
Statistics
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PSSM-Id: 153109
View PSSM: cd01050
Aligned: 30 rows
Threshold Bit Score: 295.33
Threshold Setting Gi: 158315758
Created: 24-Sep-2002
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
diiron centerhomodimerputative
Conserved site includes 6 residues -Click on image for an interactive view with Cn3D
Feature 1:diiron center [ion binding site]
Evidence:
  • Structure:1AFR; Ricinus communis (castor bean) Stearoyl-ACP desaturase; putative diferrous form that results from interaction with ferredoxin.
    View structure with Cn3D
  • Citation:PMID 8861937
  • Structure:1OQ9; Ricinus communis (castor bean) Stearoyl-ACP desaturase complexed with acetate.
    View structure with Cn3D
  • Comment:The stearoyl-ACP desaturase - acetate complex is proposed to mimic a stable mu-1,2-peroxo intermediate closely related to the catalytically competent species present during productive turnover.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                 #     
1OQ9_A       35 QKIEIFKSLDNWAEENIl-VHLKPVEKCWQPQDFLPDPa---sDGFDEQVRELRERAKEIPDDYFVVLVGDMITEEALPT 110 Ricinus communis
1AFR_A       17 QKIEIFKSLDNWAEENIl-VHLKPVEKCWQPQDFLPDPa---sDGFDEQVRELRERAKEIPDDYFVVLVGDMITEEALPT 92  castor bean
AAA62984      6 TDLQLLHELEPVVEKYM--NRHERLHKNWNPHDYIPWSdg--kNFYALGGQDWDPEQSQLSDVAQVAMVQNLVTEDNLPS 81  Mycobacterium le...
CAB45591     19 TDARLLYALEEVVEQEL--NRHLKVAKDWMPHEYVPWTearnfPGIFEDGEAWGKEQSKVTEIGRIALVVNLLTEDNLPS 96  Streptomyces coe...
NP_854505     6 TDLQLLHELEPVVEKYL--NRHLSMHKPWNPHDYIPWSdg--kNYYALGGQDWDPDQSKLSDVAQVAMVQNLVTEDNLPS 81  Mycobacterium bo...
NP_335274     6 TDLQLLHELEPVVEKYL--NRHLSMHKPWNPHDYIPWSdg--kNYYALGGQDWDPDQSKLSDVAQVAMVQNLVTEDNLPS 81  Mycobacterium tu...
ZP_04025593   6 THLELLNELSGVAEANV--NRHLSMTKDWNPHDYVPWDeg--sNYAALGGQDWDPSQSQLSDVARAAMITNLLTEDNLPS 81  Tsukamurella pau...
ZP_00996314   7 TQTDLLRELSPVVEANL--NRHIGVAKTWYPHDFIPWSdg-rnFDGLMGGDPWSPDDAGISDVARSALIVNLLTEDNLPS 83  Janibacter sp. H...
ZP_04683645  12 DDARVLHDLEPVAADAL--DDHLRHCGDWHPHEYIPWSqg-rtFDGVLDGEPWRPEQARLDPAARDGLVHNLLSEDNLPS 88  Streptomyces gha...
ZP_04293860   2 LTNDLDFRLEPRLKELYeqHKIRAQKIDWGYHEFLPWD-----KGMDFKRVPWDESQVTLPSGVITAIETALLTEVNLPW 76  Bacillus cereus ...
Feature 1                                       #  #                                            
1OQ9_A      111 YQTMLNTLDGVrdetgasptSWAIWTRAWTAEENRHGDLLNKYLYLSGRVDMRQIEKTIQYLIGSGMDPRTENSp----- 185 Ricinus communis
1AFR_A       93 YQTMLNTLDGVrdetgasptSWAIWTRAWTAEENRHGDLLNKYLYLSGRVDMRQIEKTIQYLIGSGMDPRTENSp----- 167 castor bean
AAA62984     82 YHREIAMTMGMd-------gAWGQWVNRWTAEENRHGIALRDYLVVTRAVDPVELEKLRIEVVNRGFSPGQNHQgglfad 154 Mycobacterium le...
CAB45591     97 YHHEIASLFGRd-------gAWGTWVHRWTAEEGRHGIVMRDYLLASRAVDPDKLEQFRMAHMSEGFESDNRHSm----- 164 Streptomyces coe...
NP_854505    82 YHREIAMNMGMd-------gAWGQWVNRWTAEENRHGIALRDYLVVTRSVDPVELEKLRLEVVNRGFSPGQNHQghyfae 154 Mycobacterium bo...
NP_335274    82 YHREIAMNMGMd-------gAWGQWVNRWTAEENRHGIXLRDYLVVTRSVDPVELEKLRLEVVNRGFSPGQNHQghyfae 154 Mycobacterium tu...
ZP_04025593  82 YHREISENFSMd-------hAWGHWVGRWTAEENRHGIAMRDYLLVTRAVDPVKLEEFRMTHMGNGVSSQGHSGagpear 154 Tsukamurella pau...
ZP_00996314  84 YHHEIALIFGRd-------dAWGEWVHRWTAEEGRHGIAMRDYMLVKRMVDPNALEDFRMTHMSQGYESAHSGEl----- 151 Janibacter sp. H...
ZP_04683645  89 YHRVIARMFTLd-------gAWGTWVNRWTVEEGRHGMAIRDYLLVTRATDPAALERARSAHVQNGYELDQTDVl----- 156 Streptomyces gha...
ZP_04293860  77 FTTYLSATFKGsl------sVITDFIHTWTSEEDQHSNLLETYLLLTRSVNPKRIHDLRKSVVEGGFEPDFHTPi----- 145 Bacillus cereus ...
Feature 1                   #                                #  #                               
1OQ9_A      186 --yLGFIYTSFQERATFISHGNTARqakehgDIKLAQICGTIAADEKRHETAYTKIVEKLFEIDPDGTVLAFADMMRkkI 263 Ricinus communis
1AFR_A      168 --yLGFIYTSFQERATFISHGNTARqakehgDIKLAQICGTIAADEKRHETAYTKIVEKLFEIDPDGTVLAFADMMRkkI 245 castor bean
AAA62984    155 tlfDSILYVTFQELATRVSHRNTGKac---nETIADQLLAKISSDENLHMIFYRDVSEAGIELAPNLAIKALHKILHn-F 230 Mycobacterium le...
CAB45591    165 --lHSVAYVAFQELATRISHRNTGHqs---gDPVCDRMLARIATDENLHMVFYRNLLKAAFELAPDLTMQAVRDVVVd-F 238 Streptomyces coe...
NP_854505   155 sltDSVLYVSFQELATRISHRNTGKac---nDPVADQLMAKISADENLHMIFYRDVSEAAFDLVPNQAMKSLHLILSh-F 230 Mycobacterium bo...
NP_335274   155 sltDSVLYVSFQELATRISHRNTGKac---nDPVADQLMAKISADENLHMIFYRDVSEAAFDLVPNQAMKSLHLILSh-F 230 Mycobacterium tu...
ZP_04025593 155 silHLVSYVTFQELATRVSHRNTGKac---nDPIADKLLQRIAADENLHMIFYRNICGAAIDLVPDQALRAVTDILTh-F 230 Tsukamurella pau...
ZP_00996314 152 --mHSLAYVSFQELATRVSHRNTGRft---nDPMADQLLQRIAADENLHMIFYRNLMGAALELDPSSAMQAIRDVVRd-F 225 Janibacter sp. H...
ZP_04683645 157 ---SSLVYVTLQELGTRVSYTNIQRls---gDPVCVKLLQRIGQDENRHMLFYRTVLTAAMRMYPDDTLSAAARVLAs-A 229 Streptomyces gha...
ZP_04293860 146 ---EAMTYTTLQELATLVFYNNVAKvss-khDPDLATLLRRLAKDETLHYAFYRDVIRTHLELEPNYCYHIANVIKN--F 219 Bacillus cereus ...
Feature 1                                                                                      
1OQ9_A      264 SMPAHLmydgrddnLFDHFSAVAQRLGVYTAK-DYADILEFLVGRWKVDKLTGLSAEGQKAQDYVCRLPPRIRRLEERA 341 Ricinus communis
1AFR_A      246 SMPAHLmydgrddnLFDHFSAVAQRLGVYTAK-DYADILEFLVGRWKVDKLTGLSAEGQKAQDYVCRLPPRIRRLEERA 323 castor bean
AAA62984    231 KMPGFLv------pEFRRKAVIIAVGGVYDIRiHLDEVVMPVLKKWRILEREDFSGEGTRLRDEIGEHLTELEAACDKF 303 Mycobacterium leprae
CAB45591    239 RMPGHGm------pGFERAAAQMAIGEVYNLRiHHDDVIQPVLRFLKVMEIDGLGPEGQAAQEELGLYMGGLDAEAAKF 311 Streptomyces coel...
NP_854505   231 QMPGFQv------pEFRRKAVVIAVGGVYDPRiHLDEVVMPVLKKWRIFEREDFTGEGAKLRDELALVIKDLELACDKF 303 Mycobacterium bov...
NP_335274   231 QMPGFQv------pEFRRKAVVIAVGGVYDPRiHLDEVVMPVLKKWRIFEREDFTGEGAKLRDELALVIKDLELACDKF 303 Mycobacterium tub...
ZP_04025593 231 EMPGAGm------pDFRRNGVLMAKHGIYDLRqHKDEVVMPVLRKWKIFERDDFGPEGQKAREELAAYLEDLDKQASRF 303 Tsukamurella paur...
ZP_00996314 226 AMPGTDi------pGFQRKAVEMAVAGIYDLRqHADDVVAPVLRFWKVWDLENLSAEGEQARLELADFMAGLDTKATRF 298 Janibacter sp. HT...
ZP_04683645 230 RAPGHQa------pGYEKMAASMAWSGIYSPVtHHESVLLPLTRHLGALEATGLGPRGARAQEEIAALLDTSAQRAAKF 302 Streptomyces ghan...
ZP_04293860 220 KMPGAVm------pDFENRMAVIAKEANYGPLqYFDQVLDVVVDYWGVKDLRPIAPLAEKARIEILEYHTRLKKIRDRF 292 Bacillus cereus A...

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