1T6T


Conserved Protein Domain Family
TOPRIM_RNase_M5_like

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cd01027: TOPRIM_RNase_M5_like 
Click on image for an interactive view with Cn3D
TOPRIM_ RNase M5_like: The topoisomerase-primase (TOPRIM) nucleotidyl transferase/hydrolase domain found in Ribonuclease M5: (RNase M5) and other small primase-like proteins from bacteria and archaea. RNase M5 catalyzes the maturation of 5S rRNA in low G+C Gram-positive bacteria. The TOPRIM domain has two conserved motifs, one of which centers at a conserved glutamate and the other one at two conserved aspartates (DxD). The conserved glutamate may act as a general base in nucleotide polymerization by primases. The DXD motif may co-ordinate Mg2+, a cofactor required for full catalytic function.
Statistics
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PSSM-Id: 173777
View PSSM: cd01027
Aligned: 29 rows
Threshold Bit Score: 63.0451
Threshold Setting Gi: 51247591
Created: 1-Nov-2000
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
putative activeputative metal
Conserved site includes 6 residues -Click on image for an interactive view with Cn3D
Feature 1:putative active site [active site]
Evidence:
  • Comment:By similarity to Escherichia coli DnaG primase
  • Comment:involved in nucleotide polymerization.
  • Comment:reaction is Mg2+ dependent

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1              ##  #                                                  # # #             
1T6T_2       20 REAVILVEGKNDKKALSKFSi-----kNVIDLSGKRYa----dVVDXLEGk---wEKVILLFDLDTHGERINQKXKELLs 87  Aquifex aeolicus
AAB99642     33 EEKPIIVEGKRDVESLEKLGve---gtFIIIAKTPIYl----iADELVRKr---vKEVILLTDFDRRGRMLAKAIIEEFr 102 Methanocaldococc...
NP_764153     4 VNKVIIVEGKSDKKRVQQVIae---paNIICTHGTMSidkidnMIETLYD-----KQVYVLADSDDEGEKIRKWFKRYLs 75  Staphylococcus e...
AAM07446     27 KGAVIIVEGKRDILSMKRLGid---gnFELATRHSLFn-----FSERIAKl---gCEVIILTDWDRRGDILATKLSEYFg 95  Methanosarcina a...
CAF30904     23 NGIPILVEGPNDILSLKNLKira--nfITVSNTSVFQi-----ADDLIAKn---iSEVILLTDFDRAGREYAKNIMEEFq 92  Methanococcus ma...
ZP_00560096   1 MAKVIIVEGKTDKERLQEVLae---pvEILCSYGTISyekteeWATQLED-----SEVYLLVDADDSGEKIRKSLNQALp 72  Desulfitobacteri...
ZP_00563829  44 NGAIILAEGKRDIISLKKLGi-----nGPIELVTQSPlt--eiSERVASEk----RRVIILTDWDRRGNILAAKLSEDLv 112 Methanococcoides...
BAA30813     19 FEGAIIVEGARDEASLRKLGv-----rAEIIKLSRLPl----pEVALIASe---yDEVMILTDLDRKGEELARKLAWYLe 86  Pyrococcus horik...
NP_275789     7 VDVRIIVEGASDVESVSRALqrvslgaKYHITISSIVpt---tSLEIALRavegaDIVLIATDVDQTGRELADKFREALr 83  Methanothermobac...
NP_877869     4 VDVRILVEGASDVEVISKALqglalgsEYNITISSIIpt---tNIEIAKSaaagaDLLIIATDADRVGRELAEKLFNELs 80  Pyrococcus horik...
Feature 1                       
1T6T_2       88 sqg--flvDENFRNFL 101 Aquifex aeolicus
AAB99642    103 hrgikvntKIRHEIFI 118 Methanocaldococcus jannaschii DSM 2661
NP_764153    76 e------sEHIYVDKT 85  Staphylococcus epidermidis ATCC 12228
AAM07446     96 nfg--ikpELQIRNKL 109 Methanosarcina acetivorans C2A
CAF30904     93 -------sRGIKVNNL 101 Methanococcus maripaludis S2
ZP_00560096  73 ni----hhLYTHRMYR 84  Desulfitobacterium hafniense DCB-2
ZP_00563829 113 ylg--advDMQLRDKL 126 Methanococcoides burtonii DSM 6242
BAA30813     87 gygckvdtETRRELKM 102 Pyrococcus horikoshii OT3
NP_275789    84 g-----hvGHIERMKL 94  Methanothermobacter thermautotrophicus str. Delta H
NP_877869    81 e-----mvGHIERMKL 91  Pyrococcus horikoshii OT3

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