1FOK,2FOK


Conserved Protein Domain Family
FokI_N

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cd00941: FokI_N 
N-terminal DNA recognition domain of restriction endonuclease FokI and similar proteins
Restriction endonuclease FokI (EC3.1.21.4), also called R.FokI, or endonuclease FokI, is a type IIS restriction enzyme that require only divalent metals (such as Mg2+ or Mn2+) as cofactors to catalyze the hydrolysis of DNA. FokI recognizes the double-stranded sequence 5'-GGATG-3'/3'-CATCC-5' and cleaves 14 bases after G-1 and 13 bases before C-1, respectively. It contains an N-terminal DNA recognition domain and a C-terminal endonuclease domain. This model describes the DNA recognition domain. The family also includes endonuclease StsI, a type IIS restriction endonuclease found in Streptococcus sanguinis 54. It recognizes the same sequence as FokI but cleaves at different positions.
Statistics
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PSSM-Id: 411993
Aligned: 46 rows
Threshold Bit Score: 400.794
Created: 14-Jul-2003
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
 
DNA binding
Conserved site includes 34 residues -Click on image for an interactive view with Cn3D
Feature 1:DNA binding site [nucleic acid binding site]
Evidence:
  • Structure:1FOK; Planomicrobium okeanokoites restriction endonuclease FokI in complex with DNA, contact at 4A.
    View structure with Cn3D
  • Citation:PMID 9214510

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1        #    #####   #                                                     ##    #      
1FOK_A         4 TFGWVQNPGKf-eNLKRVVQVFdRNSKVHNEVKNikiptLVKESKIQKELVAIMNqh-----dlIYTYKELVGTGTsirs 77  Planomicrobium ...
AQQ70389       3 PRGWIQDSGSf-eNLIKVVELFdKNSTTNKLLTNkfirdKVLNLDCQEYLVKSLLnedgyknnpLIEYKALVGSRTnk-- 79  Phycisphaerae b...
P29346        15 AFGLGQDVSQdlkELVKVASIFmPDSKIHKWLIDtrleeVVTDLNLRYELKSVITnt-----piSVTWKQLTGTRTk--- 86  Streptococcus s...
KRN20543       1 MNSKIQTEIKesfEWRFIDDDVlKEKLFNKLTTNp----VTLTVDDLSAEQKKEPgkpaswrikSILPAILKGQKPa--- 73  Pediococcus cla...
WP_126656953  21 TFGLGQDVSTdllDIVRVPALFiKNSKIHKELEEtrlkqVTLDAGAPKEIYDKLLnal---skdDISDSELVSLGSkygr 97  Bifidobacterium...
PKP60790       5 STGWIQDPGRl-eNLIKVVELFdHNSQTHQDLLKylilhKVLPQDGMQTLINELWsrngynnypRITYKSLVGTAFtprs 83  Candidatus Atri...
WP_086440817   4 PRGWIQDSGSl-eNLIKVVELFdKNSSTYFDILEnqilqKIDNVTKQNQLLEVLQningfngnpLIDYNSLVGSRTen-- 80  Flavobacterium ...
WP_054680412  16 STGLGQDVGSl-rSMIDMLQVFiIDSPIQNKVKEkmnwqFGDNPELKETILSKLSttpvqlttdELASEYPKKTGIpa-- 92  Lactobacillus s...
WP_135469683  16 SYGLGQDASTdllKIARVPALFvRDSAINSDLRNgr-mlRVTNDAAVTQKLLELIdrd----eiSLAPNDLIGSRTp--- 87  Weissella confusa
WP_152700119  14 TFGLGQDVAHdltKIVRVPALFiNNSQVVNELRHgkleeLTTKSGAPTEIYEELNevl---gkdKLTKKQLVSLKDgr-- 88  Lactobacillus r...
Feature 1                     ##  #                   ## ##   # #                                
1FOK_A        78 eaPCDAIIQATIADQGNKkg----------------yIDNWSSDGFLRWAHALGFIEYINksDSFVITDVGLAYSKSad- 140 Planomicrobium ...
AQQ70389      80 -eEVDGLIQVLIPGQSRLg------------------IVDWACDNFIRLAYTFNYLQYSEknDSFSITEVGLKLANAn-- 138 Phycisphaerae b...
P29346        87 -rEANSLVQAVFPGQCSRl-----------------aIVDWAAKNYVSVAVAFGLLKFHRadKTFTISEIGIQAVKLyds 148 Streptococcus s...
KRN20543      74 -rNQDNTLATDNKGESFKs-----------------sMRQWPIRNFIGEAVGLGLLDWDRktGNVTITTIGKKLANAphn 135 Pediococcus cla...
WP_126656953  98 kpYASGLIRAIFDGQRGPa------------------IKDWPAKNYLAVAVGMGLVDLDYttDTYHITDLGKEAVALldk 159 Bifidobacterium...
PKP60790      84 saRCNGIVQALIPGQRRAf------------------ISDWPANNFIRWAETLGFIQYYPvdDAYSITSFGLRLTQDn-- 143 Candidatus Atri...
WP_086440817  81 -tIVDSLVQVLLPGQNRIg------------------IVDWACDNFIRFAYTLNFVSFNEqtDSFTITEFGLELSQSe-- 139 Flavobacterium ...
WP_054680412  93 twKIKSVLPAIMDGQSIAknedgstptnpdgdtfktpMRAWPIRNFFSSAIGLGLLNWNRinGNISITTLGEQLATAshs 172 Lactobacillus s...
WP_135469683  88 -rYADSLVQAVFDGQGQRf-----------------aIVNWAAENYVSLAVTFGLLDLDRttDLYFITELGRQAVSLldn 149 Weissella confusa
WP_152700119  89 kpYATGLIRALYQGQRSPv------------------IVDWAATNYLSVAVGLGLVDYDYaaDIYTVTNLGREAVTLldq 150 Lactobacillus r...
Feature 1                                                                 ##                     
1FOK_A       141 ----gSAIEKEILIEAISSYPPAIRILTLLed-------gqHLTKFDLGKNLGFSGESGFTSLpEGILLDTLANam---- 205 Planomicrobium ...
AQQ70389     139 ----nLEEKFEIIKHSLLSYPPVTRILELLnvqyq-nsqepSLTKYEIGRELGFKGEAGFTSYsQKTVVHALSCaes--- 210 Phycisphaerae b...
P29346       149 ---eeLAELDKFLYERLLEYPYAAWLIRLLgnq-----pskQFSKFDLGEHFGFIDELGFETApIEIFLNGLAQaeidgd 220 Streptococcus s...
KRN20543     136 ddeelTLKETEIFQQTFISYPYAVGFLKALqg-------tkGLNKFQLGERFGFAGEAGFTSFgEELYINGMIEayennd 208 Pediococcus cla...
WP_126656953 160 ---ndLDNLRNFMLERLYEYPYAAWLIRVVntd-----rnkWYTKFDLGDNFGFIDEPGFSSLpEDLYVDAMFEaeyskn 231 Bifidobacterium...
PKP60790     144 ----nVNAKFNVLKEALLQYPPVTRILELLyieynkipknpLLTKFELGRELGFRGEDGFTNYpQHLVVDAIVTnl---- 215 Candidatus Atri...
WP_086440817 140 ----nDDDKFEIIKTAFKKYPPIVRVLDLLysqyenepqtpNLTKFEIGRELGFKGEDGFSTYsQNVFIQALNSaet--- 212 Flavobacterium ...
WP_054680412 173 eseelTNEELTILQLSFMSYPYAVAFLKSLeds------keGLNKFQLGESFGFAGEAGFTSFgEELYVSALTEayedgd 246 Lactobacillus s...
WP_135469683 150 ---edLDSLTNFMQERLLEYPYAAWFMRFAkkkld--egqgSFSKFDAGAEFGFLDARGFGSLpVRTFEMALARatlagd 224 Weissella confusa
WP_152700119 151 ---nkEDILNKFMLERLYEYPYAAWLIRLMnqn-----rkkKFSKFDLGANFGFIDEPGFISLpEDLYVDAMLDakanqd 222 Lactobacillus r...
Feature 1                #### #  #  ##      #       #                    #                       
1FOK_A       206 pkDKGEIRNNWEGSSDKYARMIGGWLDKL--GLVKQGKKEFIIptlgkpdnkEFISHAFKITGEGLKVLRRAKGSTKFTR 283 Planomicrobium ...
AQQ70389     211 npERTKIKNNWEGSSDKYARMISKWLCHNqvGWVQTARKKITVqige--kkfTSQLKSYQITLEGIKIFKLSRAHSRHPG 288 Phycisphaerae b...
P29346       221 ktAAQKIKSNFESTSDKYMRWLAGVLVTA--GLATSTTKKVTHtykn-rkfeLTLGTVYQITAKGLTALKEVNGKSRYPR 297 Streptococcus s...
KRN20543     209 qkLISQIRSNWESTSDKYIRGLANVLKKL--NLVNIDAQVIPYtnkqtnekeEFAIPIYKITGIGRQQLGIALGKSSHSR 286 Pediococcus cla...
WP_126656953 232 kkLKSQIKSNYESTADKYMRWLAGVLVKY--NLLERKKKVYTRiidg--keySVSLTSYKVTLQGVRSLNRVNGGSRFSR 307 Bifidobacterium...
PKP60790     216 -rQKNQILTDWEGSSDKYARMICGWLKHQkiNWVEQSVKTVSVnigg-qafaTDIPQSYKITIDGINAFRMSRARSRHRR 293 Candidatus Atri...
WP_086440817 213 tfEKSKIRQNWEGSSDKYARMICGWLSNSqiGWVVKTRKTVTIqign--erfTEILQAYQISISGITTFRSCRAYSRNAG 290 Flavobacterium ...
WP_054680412 247 raRIKKIKSNWESTSDKYIRGIASLLSKL--KLISIVDQLYSYespktgrtsHFSLPAYKLTGYGRENLGIALGRSKHKR 324 Lactobacillus s...
WP_135469683 225 fdKVKTIRSNYESTGDKYMRWIAGVLTNY--GYLKKIQNVKKTvqyygesveIGVGLGYEITDEGLTVLRQFNGGSRYRR 302 Weissella confusa
WP_152700119 223 kkEMSRIRSNYESTADKYMRWLAGVLLEY--KLIKKDSKTYHRiing--ksvEIKVPSYSLTLNGVRALNKVDGGSRFKR 298 Lactobacillus r...
Feature 1                   ##                                                                   
1FOK_A       284 VPKRVYWEMLATNltDKEYVRTRRALILEILIKag-slKIEQIQDNLKKLGFd-----------eVIETIENDIKGLINT 351 Planomicrobium ...
AQQ70389     289 VEKSVGFEMLSTKenARNFLRLRRAYILTSIKNt---kNLAQIQDYLKANSMna----------vSCETIKDDLDNFARI 355 Phycisphaerae b...
P29346       298 SRKRVMWEFLATKdkEAIAKKTSRSLMLKHLTEkk--nPIQAEVIATLINTDypt-------leiTPEEVIDDCIGLNRI 368 Streptococcus s...
KRN20543     287 STKNVTWDMLATKg-NITYIRTVRALILKALSEs---nKLTSIELADKVNNNrvnknqvldgitvSPEEIEDHIAGLNGI 362 Pediococcus cla...
WP_126656953 308 SIKRVRWEYLAPKveNAPKRKTARALMLKFLSEap--nGMTAEDMSVKINAInss-------lnsIADQVLDDAKGLNRL 378 Bifidobacterium...
PKP60790     294 KTKRVFFEMLATKgtDKGYLRTRRALIIQYLKSw---hTPYQIQTHLENRGFnn----------tSINIIKYDIHNFTRI 360 Candidatus Atri...
WP_086440817 291 TIKNVSFEMLATKglDKDFLRIRRTYILQVINQp---kSIEQIIRYLESNNVpn----------iSEETIKDDIKNFKRL 357 Flavobacterium ...
WP_054680412 325 TTKNVTWDMLATKg-NVVYTRTVRALILMELDNs---sGLTADQIATRINENkvnkmpifegatvTPEEISDHINGLNGI 400 Lactobacillus s...
WP_135469683 303 SIKRVRWEFFGPK--VDSTVKTRRALLLKYLQEaptglTTAILLEKIQADYPdl---------lvNPEMLDDDIAGFNNI 371 Weissella confusa
WP_152700119 299 STKRVMWEYLAPKaeNAQKKKTSRALMLKFLSEss--nGLSIEKLADKINAVeps-------iavIPEQVKDDAIGLNRI 369 Lactobacillus r...
Feature 1                                    
1FOK_A       352 GIFIEIKg---rFYQLKDHILQFVIPNR 376 Planomicrobium okeanokoites
AQQ70389     356 GLDIAFSn---nKYKIRDKIINLEIPQD 380 Phycisphaerae bacterium SM-Chi-D1
P29346       369 GIEILIDg---dKLTLNDKLFDFEIPVQ 393 Streptococcus sanguinis
KRN20543     363 GLQIDSDsskvkKYTLVDRLADFEIPIN 390 Pediococcus claussenii
WP_126656953 379 GIEISIDn---kVLKLKEKLYDFIIPVK 403 Bifidobacterium animalis
PKP60790     361 GLNVLSNkq--nQFRISDNIINLQIPTL 386 Candidatus Atribacteria bacterium HGW-Atribacteria-1
WP_086440817 358 GLEITFSa---nKFKLSDNIALLEIPTN 382 Flavobacterium psychrophilum
WP_054680412 401 GIQIDKNan--nKYVLKDPIQDFEIPIT 426 Lactobacillus sharpeae
WP_135469683 372 GIEIVYNva-sqKYELKDKVYDFDIPVL 398 Weissella confusa
WP_152700119 370 GIEITIDg---nHLVLKESLYDFVIPVT 394 Lactobacillus reuteri

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