5EAT,1DGP,1DI1,1JFA,1JFG,1KIZ,1N1B,1N1Z,1N24,1PS1


Conserved Protein Domain Family
Terpene_cyclase_C1

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cd00868: Terpene_cyclase_C1 
Click on image for an interactive view with Cn3D
Terpene cyclases, Class 1
Terpene cyclases, Class 1 (C1) of the class 1 family of isoprenoid biosynthesis enzymes, which share the 'isoprenoid synthase fold' and convert linear, all-trans, isoprenoids, geranyl (C10)-, farnesyl (C15)-, or geranylgeranyl (C20)-diphosphate into numerous cyclic forms of monoterpenes, diterpenes, and sesquiterpenes. Also included in this CD are the cis-trans terpene cyclases such as trichodiene synthase. The class I terpene cyclization reactions proceed via electrophilic alkylations in which a new carbon-carbon single bond is generated through interaction between a highly reactive electron-deficient allylic carbocation and an electron-rich carbon-carbon double bond. The catalytic site consists of a large central cavity formed by mostly antiparallel alpha helices with two aspartate-rich regions located on opposite walls. These residues mediate binding of prenyl phosphates via bridging Mg2+ ions, inducing proposed conformational changes that close the active site to solvent, stabilizing reactive carbocation intermediates. Mechanistically and structurally distinct, class II terpene cyclases and cis-IPPS are not included in this CD. Taxonomic distribution includes bacteria, fungi and plants.
Statistics
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PSSM-Id: 173837
View PSSM: cd00868
Aligned: 91 rows
Threshold Bit Score: 92.4288
Threshold Setting Gi: 15235504
Created: 6-Mar-2002
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 6 residues -Click on image for an interactive view with Cn3D
Feature 1:substrate binding pocket [chemical binding site]
Evidence:
  • Comment:serves as a template to channel and stabilize the conformations of reactive carbocation intermediates through a complex cyclization cascade
  • Structure:5EAT; Nicotiana tabacum Terpene cyclase, 5-epi-aristolochene synthase, with Mg2+ and substrate analog Farnesyl hydroxyphosphonate.
    View structure with Cn3D
  • Citation:PMID 9295271
  • Structure:1N24; Salvia officinalis Terpene cyclase, (+)-Bornyl diphosphate synthase with Mg2+ and product.
    View structure with Cn3D
  • Structure:1JFG; Fusarium sporotrichioides Trichodiene synthase-Diphosphate complex
    View structure with Cn3D
  • Structure:1DGP; Penicillium roquefortii Aristolochene synthase-Farnesol complex
    View structure with Cn3D
  • Citation:PMID 9295272

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                #                           #  #       
5EAT        239 LHKQELAQVSRWWKdldfvttlp-------yardrvveCYFWALGVYFEpq---ySQARVMLVKTISMISIVDDTFDAyg 308 common tobacco
1JFA_B       35 NLHYAYNKAAHHFAqprqqqllkvd---pkrlqaslqtIVGMVVYSWAKv----sKECMADLSIHYTYTLVLDDSKDDpy 107 Fusarium sporotr...
1JFG_B       35 NLHYAYNKAAHHFAqprqqqllkvd---pkrlqaslqtIVGMVVYSWAKv----sKECMADLSIHYTYTLVLDDSKDDpy 107 Fusarium sporotr...
1KIZ_B       35 NLHYAYNKAAHHFAqprqqqllkvd---pkrlqaslqtIVGMVVYSWAKv----sKECMADLSIHYTYTLVLEDSKDDpy 107 Fusarium sporotr...
AAC49957     35 NLSYANNKAAAHFAqerqqrilkvn---pkrleaslrtIVGMVVYSWVKv----sKELMADLSIHYTYTLILDDSEDDph 107 Myrothecium roridum
BAB39207     16 NSAEAESAYSSIIAaldlqesdy-------avisghsrIVGAAALVYPDa----dAETLLAASLWTACLIVNDDRWDYvq 84  Kitasatospora gr...
ZP_00085244  67 LGQEVNERLMEWISnigifagk--------eekirasdFGRYAMLCHADtn---nPDRLLLVAQCFAALFAVDDHYCDdq 135 Pseudomonas fluo...
AAO50995     18 ETKPSKIEYMQFVYqeaidmkvwrk--dneidilthhhVTDLSRFFWPNa----dFEGLVLGAELMVWFFAFDDLFDGgf 91  Dictyostelium di...
AAO51481     31 LKEESFTTFKPIVYekylnwtqsieesilktngtinksIFEEIFSYCGYigefleYEPFIVFLKFTTFGIILDDYIFEki 110 Dictyostelium di...
AAO52111     29 NYKHIKDTYNEGVScgifeasne-------khfayangVLNCVTWFYPKy----dYEQCMVAASIMLWIFVLDDFLERdh 97  Dictyostelium di...
Feature 1                                                                                       
5EAT        309 tvkeleaytdaiq-------rwdineidrlpdymkISYKAILDLYKDYEkelssag----rshivcHAIERMKEVVRNYN 377 common tobacco
1JFA_B      108 ptmvnyfd-----------------dlqagreqahPWWALVNEHFPNVLrhfgp--------fcslNLIRSTLDFFEGCW 162 Fusarium sporotr...
1JFG_B      108 ptmvnyfd-----------------dlqagreqahPWWALVNEHFPNVLrhfgp--------fcslNLIRSTLDFFEGCW 162 Fusarium sporotr...
1KIZ_B      108 ptmvnyfd-----------------dlqagreqahPWWALVNEHFPNVLrhfgp--------fcslNLIRSTLDFFEGCW 162 Fusarium sporotr...
AAC49957    108 nnmltffd-----------------dlqagreqkhPWWMLVNEHFPNVLrhfgp--------fcslNLIRSTLDFFEGCW 162 Myrothecium roridum
BAB39207     85 edggrlapgewfd--------gvtevvdtwrtagpRLPDPFFELVRTTMsrldaal----gaeaadEIGHEIKRAITAMK 152 Kitasatospora gr...
ZP_00085244 136 slggrpetvaeslsf---altaidpvylpspfdkeLLKQQMCDPVIRGLlaymkrvaqfctpsqvaRVRQITIAMFVTMA 212 Pseudomonas fluo...
AAO50995     92 iddneneqyrlvnrm---nkvflegtieddstgaeRMGYYLRNKVRAICgekrq--------stfhRFNSSCVQWVDSII 160 Dictyostelium di...
AAO51481    111 nslnmklnekek---------linsliyynnknenKIGFEFWEIINEFQnfthk--------esfqRIINANSLWIKSSI 173 Dictyostelium di...
AAO52111     98 mtdekqqycvkkyedilifgkdssyisnieyndltPLDKYSLILRKRLLnptihr------iesfnIFIHYLREWFFSII 171 Dictyostelium di...
Feature 1                                                                             ##  #     
5EAT        378 VEstwfie--gytppvSEYLSNALAtttyYYLATTSYlgmksateq--dfewlsknpKILEASVIICRVIDDTATYEVEk 453 common tobacco
1JFA_B      163 IEqynfgg---fpgshDYPQFLRRMnglgHCVGASLWpkeqfner-----slfleitSAIAQMENWMVWVNDLMSFYKEf 234 Fusarium sporotr...
1JFG_B      163 IEqynfgg---fpgshDYPQFLRRMnglgHCVGASLWpkeqfner-----slfleitSAIAQMENWMVWVNDLMSFYKEf 234 Fusarium sporotr...
1KIZ_B      163 IEqynfgg---fpgshDYPQFLRRMnglgHCVGASLWpkeqfner-----slfleitSAIAQMENWMVWVNDLMSFYKEf 234 Fusarium sporotr...
AAC49957    163 IEqynfhg---fpgsyDFPGFLRRMnglgHCVGGSLWpkelfdeq-----khfleitSAVAQMENWMVWVNDLMSFYKEf 234 Myrothecium roridum
BAB39207    153 WEgvwney--tkktslATYLSFRRGyctmDVQVVLDKwinggrsfa--alrddpvrrAIDDVVVRFGCLSNDYYSWGREk 228 Kitasatospora gr...
ZP_00085244 213 AEgpwrly--gtqptvAEYLASRQVns-fWPCLVLIDliggyevpa--ntysrpdihHVTALASLATTLVNDLYSAYKEh 287 Pseudomonas fluo...
AAO50995    161 PFiklkrn--qksldfNLYIHHRKFnigaIPCFLVSEiildpmsniecfiwldsrwiKMSEIICEIIALVNDCVSYEKEi 238 Dictyostelium di...
AAO51481    174 DSrnisnspinsrcsfNEYFEKRSSdasgDFILTISMigimdnyien-siieskefqTINYHAKSFFLLINDLYSFNREi 252 Dictyostelium di...
AAO52111    172 PLkkskgnn-hdsvpiEVYQFIRTIniglFFVVGVNSvavrtnvhg--sfwlnpiwnRMVRSASRQIIIFNDCVSYAKEl 248 Dictyostelium di...
Feature 1                                                                                       
5EAT        454 --SRGQi-aTGIECCMRdygistKEAMAKFQNMAETAWKDINEGLlrpt-----------pvsTEFLTPILNLARIVEVT 519 common tobacco
1JFA_B      235 --DDERdqiSLVKNYVVsdeislHEALEKLTQDTLHSSKQMVAVFsd--------------kdPQVMDTIECFMHGYVTW 298 Fusarium sporotr...
1JFG_B      235 --DDERdqiSLVKNYVVsdeislHEALEKLTQDTLHSSKQMVAVFsd--------------kdPQVMDTIECFMHGYVTW 298 Fusarium sporotr...
1KIZ_B      235 --DDERdqiSLVKNYVVsdeislHEALEKLTQDTLHSSKQMVAVFsd--------------kdPQVMDTIECFMHGYVTW 298 Fusarium sporotr...
AAC49957    235 --DDPRdqtSLVKNYVVcdeislTQALEKLTVDTLTSSEQMMEVFsd--------------kdAKLMETIECFMHGYITW 298 Myrothecium roridum
BAB39207    229 -kAVDKs--NAVRILMDhagydeSTALAHVRDDCVQAITDLDCIEesikrsgh--lgshaqelLDYLACHRPLIYAAATW 303 Kitasatospora gr...
ZP_00085244 288 lnETGDf--KLPYLLAArhncslQEAIDLAADIHDAVMEEYERLHatlmkg------trspvlRRYLTGLSTWIGGNLEW 359 Pseudomonas fluo...
AAO50995    239 -kENGAp-lNSLKFIQIeknlnlQESFEYISNYLNELINQYIELEtsfiksykpitsnynsnfIAIVEHLHNMSFANVSW 316 Dictyostelium di...
AAO51481    253 --NENDl-lNYIKILAIqlnsiqLSIDKTIELIIDHYLKFLSSIEtilklyqn--dqstyqllKQVFQNSNKILSGIYFA 327 Dictyostelium di...
AAO52111    249 -nHDCAg-eNCLYILQKklnlpfEKVYSELIAEAKQCIFDIQNDEkllikslshiseeemngvIYLVEQLHEIICGNRDW 326 Dictyostelium di...
Feature 1        
5EAT        520 Y 520 common tobacco
1JFA_B      299 H 299 Fusarium sporotrichioides
1JFG_B      299 H 299 Fusarium sporotrichioides
1KIZ_B      299 H 299 Fusarium sporotrichioides
AAC49957    299 H 299 Myrothecium roridum
BAB39207    304 P 304 Kitasatospora griseola
ZP_00085244 360 H 360 Pseudomonas fluorescens PfO-1
AAO50995    317 S 317 Dictyostelium discoideum
AAO51481    328 H 328 Dictyostelium discoideum
AAO52111    327 A 327 Dictyostelium discoideum

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