1F4L,2CSX,1A8H,1RQG


Conserved Protein Domain Family
MetRS_core

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cd00814: MetRS_core 
Click on image for an interactive view with Cn3D
catalytic core domain of methioninyl-tRNA synthetases
Methionine tRNA synthetase (MetRS) catalytic core domain. This class I enzyme aminoacylates the 2'-OH of the nucleotide at the 3' of the appropriate tRNA. MetRS, which consists of the core domain and an anti-codon binding domain, functions as a monomer. However, in some species the anti-codon binding domain is followed by an EMAP domain. In this case, MetRS functions as a homodimer. The core domain is based on the Rossman fold and is responsible for the ATP-dependent formation of the enzyme bound aminoacyl-adenylate. It contains the characteristic class I HIGH and KMSKS motifs, which are involved in ATP binding. As a result of a deletion event, MetRS has a significantly shorter core domain insertion than IleRS, ValRS, and LeuR. Consequently, the MetRS insertion lacks the editing function.
Statistics
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PSSM-Id: 173907
View PSSM: cd00814
Aligned: 54 rows
Threshold Bit Score: 349.138
Threshold Setting Gi: 23483588
Created: 24-Sep-2002
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 10 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1           ## #                                       #                              
1F4L_A      7 KILVTCALPYANGSIHLGHMLeHIQA-DVWVRYQRMRG--HEVNFICADDAHGTPIMLKAQQLGitPEQMIGEMSQEHQT 83  Escherichia coli
NP_586395   2 KKFITSALPYVNNQPHLGNIIgSVLSgDVYSRYCKKKG--EVSVYICGTDEYGTAIEMEAISQGvtPLEICEKNRKLHKQ 79  Encephalitozoon cu...
Q8ZU56      3 KYVIGSAWPYVQTVPHLGNMIgSVLSaDVYARYLRLKG--HEVVFVSGSDVHGTPVEVEAIQLGvdPAEYSMKMHAIVAE 80  Pyrobaculum aeroph...
Q9YCY3      3 KYVVTSAWPYVNHVPHLGTLIgSVLSaDIYARYLRLRG--RQVVFVSGSDEHGTPIELEARKKGvhPKELTDQVHEYDVK 80  Aeropyrum pernix
Q44951      4 MNLVTAALPYVNNIPHLGNLVqVLSA-DAFARYSKMSG--IETLYVCGTDEYGTATETKALIENttPLELCNKYYEIHKS 80  Lyme disease spiro...
Q9Z959      4 KVLITSALPYANGPLHFGHIAgVYLPaDVYARFRRLLG--DDVLYICGSDEFGIAITLNADREGlgYQEYVDMYHKLHKD 81  Chlamydophila pneu...
Q9UWW2      2 KVLVTAAWPYVNSVPHLGNLIgSILSaDVFARYARLRYgkENVLFVSGSDEHGTPIEIEAIKRKvnPKELTDQAHEYDRH 81  Sulfolobus solfata...
O83776      3 RKLITAALPYVNNVPHLGNLI-QGLSaDVFARFCRMRG--YHTCFVCGTDEYGTASETRAAEQGlsPAQLCAHYHALHRD 79  Treponema pallidum
AAO25636    2 SIFIGGAWPYANGSLHIGHAAaLLPG-DILARYYRQKG--EEVLYVSGSDCNGTPISIRAKKENksVKEIADFYHKEFKE 78  Streptococcus pneu...
NP_825580   3 RHLITSALPYINGIKHLGNMVgSMLPaDVYSRYLRQRG--HDVLYICATDEHGTPAELAAKERGlpVDEFCAQAHDAQKA 80  Streptomyces averm...
Feature 1                                                                                     
1F4L_A     84 DFAG-FNISYDNYHSTHSEENRQLSELIYSRLKenGFIKNRTISQLYDPEKGMFLPdrfvkgtcpkckspdqygdncev- 161 Escherichia coli
NP_586395  80 VYDW-FNIDFDYFGFTSSATHTGLVQDLFMKMYdnGHFSEVEIEQFYCEHCGLFLAdrfivgeckfcgdgrargdqcds- 157 Encephalitozoon cu...
Q8ZU56     81 LFKK-WNISFDLYTHTHSETHIKFVQDFYLKIYenGYIFTKEDEVPYCPRDKIYLPdrfiigkcpycgyerargdqcen- 158 Pyrobaculum aeroph...
Q9YCY3     81 MWRE-YRISFDNYSRTESPVHKEFVMEFMKKLEenGYIFSQEEVLPYCERDKIFLPdrfvegtcpycgyekargdqcde- 158 Aeropyrum pernix
Q44951     81 IYKW-FNIEFDIFGRTTNKNHQDIVQNFFLQLEknGYIKERETEQFYCNKDSMFLAdryvigecpecqsmakgdqcdn-- 157 Lyme disease spiro...
Q9Z959     82 TFEK-LGFALDFFSRTTNPFHAELVQDFYSQLKasGLIENRISEQLYSEQEQRFLAdryvegtcprcgfdhargdecqs- 159 Chlamydophila pneu...
Q9UWW2     82 LFLNvWKISFDNYTRTESEIHKKFVREFLLKLT--KYIKVSEDEIPYCENDKLYLPdrfvkgtcpycgfedargdqcdn- 158 Sulfolobus solfata...
O83776     80 IYQW-FDLSFDYFGRTTSDAHTELTQALFRHLDarGFISEHESAQAYCLHCARFLAdrylrgtcphcrnaearadqceh- 157 Treponema pallidum
AAO25636   79 TFEK-LGFTYDLYSRTDSPLHHEIVQELFLQLYekKFLYTKKIKQLYCTFDNQFLPdrfvegkcpncgthsrgdqcdn-- 155 Streptococcus pneu...
NP_825580  81 VYDG-FELAFDYFGRSSSEQNREITQHFARRLNenGFIEERAIRQVYSPADGRFLPdryvegtcphcgydkargdqcenc 159 Streptomyces averm...
Feature 1                                                                                     
1F4L_A    162 cgatyspteliepksvvsgatpVMRDSEHFFFDLPSFSEMLQAWTRSga----lQEQVANKMQEWFEsgLQQWDISRDAp 237 Escherichia coli
NP_586395 158 cghtynslellsprcsicssspVVRATTHLFFDLEAFRPSLEELYRTngh--lwSQNAQNIFRQWISmeFYPRCMTRDLk 235 Encephalitozoon cu...
Q8ZU56    159 cgrlldpkqliepkcavcgskpEWRITKHWYLDLRRLEDRIRKYVEEnp---hlPPNAKEMSLAMLKegLRPRAVTRDN- 234 Pyrobaculum aeroph...
Q9YCY3    159 cgrllhptelknprcalcgskpVYKSTRHWFIDLRRVQDRLLKWLEShg---elQDSVKKYSINWVAqgLKPRSVTRDL- 234 Aeropyrum pernix
Q44951    158 cskllnptdlinpkciicknkpILKKTNHLYLDLPKIKTKLEKWIKNpdtsknwNTNALKMTKAFLRdgLKERAITRDL- 236 Lyme disease spiro...
Q9Z959    160 cgadyeaidlidpkskisgvelVKKETEHSYFLLDRMKDALLSFIQGcy----lPDHVRKFVVDYIEh-VRSRAITRDL- 233 Chlamydophila pneu...
Q9UWW2    159 cgklltpsllvnpkcsicgktpVFKKTKHWFFDLSEFNDKIRGWISSsn---emPDNVKSVALSWVGegLKPRSITRDN- 234 Sulfolobus solfata...
O83776    158 cgvllepetllnarcvscgtapEFRPTRHLYLNLPALEKAYRSWFCTtnh--lwTKNAVRMTEGWLRtgLQERAITRDL- 234 Treponema pallidum
AAO25636  156 csaildpidlvdkrcsicsnepEVRETEHFYYVFSEFQNLLETYLNDaeetvrwRKNAINLTKRYLRegLPDRAVTRDL- 234 Streptococcus pneu...
NP_825580 160 trvldptdlinprsaisgstdlEVRETKHLFLLQSKLQHEVEEWVARhee--ewPQLASSIARKWLNegLHDRAITRDL- 236 Streptomyces averm...
Feature 1                             #  ##  #                                              # 
1F4L_A    238 -YFGFEIPn-------aPGKYFYVWLDAPIGYMGSFKnlcdkrgdsvs----------fdeywkkdstaELYHFIGKDIV 299 Escherichia coli
NP_586395 236 fNWGVPVPle-----kfKEKVFYVWFDAPIGYLTFLKelvgedf------------------gewckdaELVQFMGKDNV 292 Encephalitozoon cu...
Q8ZU56    235 -KWGIPAPfp-----gaEGKTIYVWFEAVLGYISAVVeyfkkigreee-----------wkrfwldpetKVVFFVGKDNV 297 Pyrobaculum aeroph...
Q9YCY3    235 -SWGVPAPfk-----gaEGKTIYVWFDALLGYVSATKelfimrrgdpe----------ewkswwwdsgtRTVYFIGKDNI 298 Aeropyrum pernix
Q44951    237 -KWGIPVPkk-----gfENKVFYVWFDAPIGYISITKniiknwes----------------wwknndqvNLVQFIGKDNI 294 Lyme disease spiro...
Q9Z959    234 -SWGIPVPd-------fPGKVFYVWFDAPIGYISGTMewaasqgnpde-----------wkrfwledgvEYVQFIGKDNL 294 Chlamydophila pneu...
Q9UWW2    235 -KWGIPAPfe-----gaQDKSIYVWFEALLGYISAVIeyferkgdqek-----------wkeywfgpniKSYYFIGKDNI 297 Sulfolobus solfata...
O83776    235 -RWGVPVPka-----gfEQKVFYVWFDAPVGYISITKcgteaassqegggtddgvkekwqswwldqqdvELVQFVGKDNI 308 Treponema pallidum
AAO25636  235 -PNGIPVPid-----gfRDKKIYVWFEAVAGYYTASVdwaqklqnn--------------itdfwnnrtKSYYVHGKDNI 294 Streptococcus pneu...
NP_825580 237 -DWGVPVPadtwpelaaEGKVFYVWFDAPIEYIGATKewsdldpenrdwk------swwydadsgenpvRYTEFMAKDNV 309 Streptomyces averm...
Feature 1       #                                                                            
1F4L_A    300 YFHSLFWPAMLEGSN--FRKPSNLFVHGYVTVN-GAKMSKSRGTFIKASTWLNHF--DADSLRYYYTAKlssrIDDIDL 373 Escherichia coli
NP_586395 293 AFHTVIFPAMLYATGekYPVVRRLSATEYLQFE-NEKFSKSRRHGIFGLDLVGGGlgKSCMWRYYLLKIrpesTKDSNF 370 Encephalitozoon cun...
Q8ZU56    298 PFHVIILPALLLANGggYVMPTTTASTEYLLYE-GDKFSKSRRWGVWIDEALQLL--PADYWRFVLIYIrp-eNRDTNF 372 Pyrobaculum aerophilum
Q9YCY3    299 PFHAIILPALFLASHdpYVLPWRISATEYLMYE-GQQFSKSRRIGVWIDEALEIA--PADYWRWALARMrp-eARDTNF 373 Aeropyrum pernix
Q44951    295 LFHTIIFPCIEIGSEenWTILNQLSSSEYLNYE-NLKFSKSEGTGIFGNDAITTGi-PSDIWRFYIYYNrp-eKSDFQF 370 Lyme disease spiroc...
Q9Z959    295 PFHSVVFPAMELGQKldYKKVDALVVSEFYLLE-GRQFSKSEGNYVDMDKFLSSY--SLDKLRYVLAATap-eTSDSEF 369 Chlamydophila pneum...
Q9UWW2    298 PFHAVILPAMLMASEeeYHLPDVIAATEYLLYE-GQKFSKSRKIGVWIDEAPELM--DVEYWRFVLIRLrp-eEKDTNF 372 Sulfolobus solfatar...
O83776    309 PFHTLFFPCMLIGSGq-RWTMLTRLSATEYLNYeGGKFSKSLGVGVFGSDAKESGi-PSDLWRFYLLYHrp-eKSDAHF 384 Treponema pallidum
AAO25636  295 PFHTIIWPAILSGLEi-EPLPEYIISSEYLTLE-NKKISTSNNWAIWLNDIIKKY--DADSIRYFLTINap-eMKDANF 368 Streptococcus pneum...
NP_825580 310 PFHTVMFPATELGVRepWKKVDYVKAFNWLTYY-GGKFSTSQKRGVFTDHALEIL--PADYWRYFLIANap-eSDDSSF 384 Streptomyces avermi...

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