1G59,2O5R,2CFO,2JA2,2CV2


Conserved Protein Domain Family
GluRS_core

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cd00808: GluRS_core 
Click on image for an interactive view with Cn3D
catalytic core domain of discriminating glutamyl-tRNA synthetase
Discriminating Glutamyl-tRNA synthetase (GluRS) catalytic core domain . The discriminating form of GluRS is only found in bacteria and cellular organelles. GluRS is a monomer that attaches Glu to the appropriate tRNA. Like other class I tRNA synthetases, GluRS aminoacylates the 2'-OH of the nucleotide at the 3' end of the tRNA. The core domain is based on the Rossman fold and is responsible for the ATP-dependent formation of the enzyme bound aminoacyl-adenylate. It contains the characteristic class I HIGH and KMSKS motifs, which are involved in ATP binding.
Statistics
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PSSM-Id: 173905
View PSSM: cd00808
Aligned: 34 rows
Threshold Bit Score: 282.168
Threshold Setting Gi: 6685993
Created: 6-Mar-2002
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 19 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:2CV2_A; Thermus thermophilus GluRS binds Glu-AMS, defined using 4.0 A contacts.
    View structure with Cn3D
  • Comment:tRNA is required for amino acid activation

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1         # ###       ## ##                   #                                       
1G59_A      1 MVVTRIAPSPTGDPHVGTAYIALFNYAWARRNGGRFIVRIEDTDRARYVPGAEERILAALKWLGLSYdegpdvaaptgpY 80  Thermus thermophilus
2O5R_A     13 XVRVRFAPSPTGFLHVGGARTALFNFLFARKEKGKFILRIEDTDLERSEREYEEKLXESLRWLGLLWdegpdvggdhgpY 92  Thermotoga maritima
2CV2_A      1 MVVTRIAPSPTGDPHVGTAYIALFNYAWARRNGGRFIVRIEDTDRARYVPGAEERILAALKWLGLSYdegpdvggphgpY 80  Thermus thermophilus
P22250      4 EVRVRYAPSPTGHLHIGNARTALFNYLFARNQGGKFIIRVEDTDKKRNIEGGEQSQLNYLKWLGIDWdesvdvggeygpY 83  Bacillus subtilis
P47700      3 KIRTRYAPSPTGYLHVGGTRTAIFNFLLAKHFNGEFIIRIEDTDTERNIKEGINSQFDNLRWLGVIAdesvynpgnygpY 82  Mycoplasma genitalium
P96551      3 LIVTRFAPSPTGYLHIGGLRTAIFNYLFARANQGKFFLRIEDTDLSRNSIEAANAIIEAFKWVGLEYdg--------eiL 74  Helicobacter pylori
O67271      3 KVKTRFAPSPTGYLHLGNARTAIFSYLFARHNNGGFVLRIEDTDPERSKKEYEEMLIEDLKWLGIDWde---------fY 73  Aquifex aeolicus
Q9ZCT8      3 NIITRFAPSPTGFLHIGSARTALFNYLFARHNNGKFFLRIEDTDKKRSTKEAVEAIFSGLKWLGLNWdg--------evI 74  Rickettsia prowazekii
Q8RB93      4 EVRVRFAPSPTGSLHIGGARTALFNWLFARHHGGKFILRVDDTDLERSTEESMRGILEGLQWLGIDWde--------gpI 75  Thermoanaerobacter...
Q8EYM3      6 EVRTRFAPSPSGFLHVGGARTALFNYLYAKSQGGKFILRIEDTDQNRSTEDSFKIILESLKWLGVNWdegpevggeygpY 85  Leptospira interro...
Feature 1                                                                                     
1G59_A     81 RQSERLPLYQKYAEELLKRGwayrafetpeeleqirkekggydgrarnippeeaeerarrg-------ephvirlkvprp 153 Thermus thermophilus
2O5R_A     93 RQSERVEIYREHAERLVKEGkayyvyaypeeieexrekllsegkaphysqexfekfdtperrreyeekglrpavffkxpr 172 Thermotoga maritima
2CV2_A     81 RQSERLPLYQKYAEELLKRGwayrafetpeeleqirkekggydgrarnippeeaeerarrg-------ephvirlkvprp 153 Thermus thermophilus
P22250     84 RQSERNDIYKVYYEELLEKGlaykcycteeelekereeqiargemprysgkhrdltqeeqekfi-aegrkpsirfrvpeg 162 Bacillus subtilis
P47700     83 LQSQKLAVYKKLAFDLIEKNlayrcfcskeklesdrkqainnhktpkylghcrnlhskkitnhl-ekndpftirlkinne 161 Mycoplasma genitalium
P96551     75 YQSKRFEIYKEYIQKLLDEDkayycymskeeldalreeqkarketprydnryrdfkgtppk------giepvvrikvpqn 148 Helicobacter pylori
O67271     74 RQSERFDIYREYVNKLLESGhaypcfctpeelekereearkkgipyrysgkcrhltpeevekfk-kegkpfairfkvpen 152 Aquifex aeolicus
Q9ZCT8     75 FQSKRNSLYKEAALKLLKEGkayycftrqeeiakqrqqalkdkqhfifnsewrdkgpstypa-----dikpvirlkvpre 149 Rickettsia prowazekii
Q8RB93     76 YQSQRLDEYRKFANKLLEEGkayycfctkeeleemrkqaeregrpfmytgkcrnltkeqienyl-kegkkpvirlktpre 154 Thermoanaerobacter...
Q8EYM3     86 IQSQRLNIYKEYTEKLLKEKkayrcfctqeeleakkkqseamgvpyvydglhanmsdeevqek--lkqgipysvrfktps 163 Leptospira interro...
Feature 1                                      #   #             ## ##                        
1G59_A    154 gttevkdelrgvvvydnqeipdvvllksDGYPTYHLANVVDDHLMGVTDVIRAEEWLVSTPIHVLLYRAFGw-EAPRFYH 232 Thermus thermophilus
2O5R_A    173 kdyvlndvvkgevvfktgaigdfvixrsNGLPTYNFACVVDDXLXEITHVIRGDDHLSNTLRQLALYEAFEk-APPVFAH 251 Thermotoga maritima
2CV2_A    154 gttevkdelrgvvvydnqeipdvvllksDGYPTYHLANVVDDHLMGVTDVIRAEEWLVSTPIHVLLYRAFGw-EAPRFYH 232 Thermus thermophilus
P22250    163 kviafndivkgeisfesdgigdfvivkkDGTPTYNFAVAIDDYLMKMTHVLRGEDHISNTPKQIMIYQAFGw-DIPQFGH 241 Bacillus subtilis
P47700    162 aeyswndlvrgqitipgsaltdivilkaNGVATYNFAVVIDDYDMEITDVLRGAEHISNTAYQLAIYQALGfkRIPRFGH 241 Mycoplasma genitalium
P96551    149 evigfndgvkgevkvntnelddfiiarsDGTPTYNFVVTIDDALMGITDVIRGDDHLSNTPKQIVLYKALNf-KIPNFFH 227 Helicobacter pylori
O67271    153 rtvvfedlikghiaintddfgdfvivrsDGSPTYNFVVVVDDALMGITHVIRGEDHIPNTPKQILIYEALGf-PVPKFAH 231 Aquifex aeolicus
Q9ZCT8    150 gsitihdtlqgeivienshiddmilirtDGTATYMLAVIVDDHDMGITHIIRGDDHLTNAARQIAIYHAFGy-EVPNMTH 228 Rickettsia prowazekii
Q8RB93    155 gktvvhdiirgdvefdnstiddfiimksDGMPTYNFATVVDDYQMGITHIIRAEEHLSNTPKQILIYEALGv-PLPQFAH 233 Thermoanaerobacter...
Q8EYM3    164 ktliindiiqgkvkfetkligdfiivksDGFPSYNYAVVVDDALMKITHVIRGVGHLSNTPRQILLYEALGy-NIPEFAH 242 Leptospira interro...
Feature 1      ##       ##                                                                    
1G59_A    233 MPLLRNpdktKISKRKsh--tSLDWYKAEGFLPEALRNYLCLMGfsmpdgreiftlee-fiqaftwervslgGPVFDLEK 309 Thermus thermophilus
2O5R_A    252 VSTILGpdgkKLSKRHga--tSVEAFRDXGYLPEALVNYLALLGwshpegkelltlee-lissfsldrlspnPAIFDPQK 328 Thermotoga maritima
2CV2_A    233 MPLLRNpdktKISKRKsh--tSLDWYKAEGFLPEALRNYLCLMGfsmpdgreiftlee-fiqaftwervslgGPVFDLEK 309 Thermus thermophilus
P22250    242 MTLIVNesrkKLSKRDesiiqFIEQYKELGYLPEALFNFIGLLGwspvgeeelftkeq-fieifdvnrlsksPALFDMHK 320 Bacillus subtilis
P47700    242 LSVIVDesgkKLSKRDekttqFIEQFKQQGYLPEALLNFLALLGwhpqynqeffnlkq-lienfslsrvvsaPAFFDIKK 320 Mycoplasma genitalium
P96551    228 VPMILNeegqKLSKRHga--tNVMDYQEMGYLKEALVNFLARLGwsyqdkevfsmqe--llelfdpkdlnssPSCFSWHK 303 Helicobacter pylori
O67271    232 LPVILGedrsKLSKRHga--vSVRAYREEGYMPEALFNYLCLLGwsppeegreifskeelikifdlkdvndsPAVFNKEK 309 Aquifex aeolicus
Q9ZCT8    229 IPLIHGadgtKLSKRHga--lGVEAYKDMGYLPESLCNYLLRLGwshgddeiismnq--aiewfnlaslgksPSKLDFAK 304 Rickettsia prowazekii
Q8RB93    234 VSMVLApdrtKLSKRHga--tSVQEFRDQGYLPEAIVNYITLLGwapldgeeifdvrk-sirefslervsknPAVYDVQK 310 Thermoanaerobacter...
Q8EYM3    243 ASEIVGmdgkKLSKRAga--tSILAFRDLGYLPETFRNYMALLGwtstdgreflpgde-lekifdvhrcsksPSTFDVFK 319 Leptospira interro...
Feature 1                
1G59_A    310 LRWMNGKYIRE 320 Thermus thermophilus
2O5R_A    329 LKWXNGYYLRN 339 Thermotoga maritima
2CV2_A    310 LRWMNGKYIRE 320 Thermus thermophilus
P22250    321 LKWVNNQYVKK 331 Bacillus subtilis
P47700    321 LQWINANYIKQ 331 Mycoplasma genitalium
P96551    304 LNWLNAHYLKN 314 Helicobacter pylori
O67271    310 LKWMNGVYIRE 320 Aquifex aeolicus
Q9ZCT8    305 MNSINSHYLRM 315 Rickettsia prowazekii
Q8RB93    311 LTWINGHYIRS 321 Thermoanaerobacter tengcongensis
Q8EYM3    320 KPKGSDEEVVT 330 Leptospira interrogans

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