Conserved Protein Domain Family
ProRS_core_prok
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cd00779: ProRS_core_prok 
Prolyl-tRNA synthetase (ProRS) class II core catalytic domain. ProRS is a homodimer. It is responsible for the attachment of proline to the 3' OH group of ribose of the appropriate tRNA. This domain is primarily responsible for ATP-dependent formation of the enzyme bound aminoacyl-adenylate. Class II assignment is based upon its structure and the presence of three characteristic sequence motifs in the core domain. This subfamily contains the core domain of ProRS from prokaryotes and from the mitochondria of eukaryotes.
Links
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Statistics
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PSSM-Id: 238402
Aligned: 31 rows
Threshold Bit Score: 326.456
Created: 24-Sep-2002
Updated: 2-Oct-2020
Structure
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Aligned Rows:
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Feature 1:active site [active site]
Evidence:
  • Comment:based upons similarity to archaeal/eukaryotic-like ProRS subtype
  • Comment:3' end of tRNA does not enter the active site
Scroll to Sequence Alignment Display
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                                                     
Q9ZMJ6     17 DAVLKSPKHPGKAGYIYQiGSGIYNFLPLAKKVLDKIENVTHKRMQEHg-AQNILMSFVVLASLWEKSGRLDKyg----k 91  Helicobacter pylor...
P36431     17 EASVLSYELLQKAGYLFKtSKGIYSYTPLFQRVILKMTEIIREELNAIg-GQEVCLPLLQPAELWEKTGRWKAfls--ek 93  Chlamydia trachomatis
AAO51960   67 SDMLESQLLMQKGGLIRRlSAGTYSTLPLAQKVMENIIKIIDEEMEKVg-GQKMSMPKMLPRELWEKTGRWDSag----d 141 Dictyostelium disc...
P78600     27 SKQIPTHEVLTKLGYITYpRAGLVNWSKMGLLIQNKISQIIRQRMDEIq-FEEVSLSLISHKELWKLTNRWDQ------- 98  Candida albicans
P39965     24 LKSETTQELLQTVGFVRRsQVGLFQWLPLGLRSLNKVSNAIRNRMDSDggAIEVSLSAISSKALWQATDRWNNs------ 97  baker's yeast
AAH44273   56 EPTSKSQKLMIKAGLIRPsNPGCFHYLPYTVRAMEKLIRLIDKEMQDIg-GQKIDMPTLGSAALWRQSGRWDLmg----k 130 African clawed frog
XP_313346  24 DITSKSQRLMLEQGLIRQaGNGTFYLLPLLQRSLQKAVDLIDRHMEAVg-AQKMVLPLLTSAELWKKSGRLSVteagtpt 102 Anopheles gambiae ...
AAF47674   24 EQLSRSQKLLTELGLVKSgSNGTYQIMPMAQRSVDKCIDLVQSNMQQAg-GQKITLPILTPTGLWKKTGRLDGdi----s 98  fruit fly
NP_689481  65 DLTCKSQRLMLQVGLIYPaSPGCYHLLPYTVRAMEKLVRVIDQEMQAIg-GQKVNMPSLSPAELWQATNRWDLmg----k 139 human
CAA21147   22 NVPESANQLLIDMGFIQPsMPGIFQYLPLGLRVQNKICDLLHISMRSLg-ASAISLAHLSSKEIWEKSGRWQKtg----s 96  fission yeast

Feature 1                      # #                             # #       ###  # # #           
Q9ZMJ6     92 eLLVFKDRkdNDFVLSPTLEENITEIAANFI-KSYKQLPVHLYQIHTKFRDEIRPRFGLVRAREFIMKDGYSFHEDAESL 170 Helicobacter pylor...
P36431     94 lLYVLKDRenKAMCLAPTHEEVVSEFVAQWL-TGREQLPIHLYQIGTKFRDEIRPRFGLMRAKEFLMEDSYTFSDSPEQM 172 Chlamydia trachomatis
AAO51960  142 dLIKLKDRkgEEYCLAPTHEEVITTIVANES-LSNSSFPIKLYQIGEKYRDEVRPRFGLLRGREFTMKDMYSFDTSKEAA 220 Dictyostelium disc...
P78600     99 -EEIFKLVg-DEYLLVPTAEEEITNYVKKQFlESYKNFPLALYQINPKFRNEKRPRGGLLRGKEFLMKDAYSFDLNESEA 176 Candida albicans
P39965     98 eLFKLKDSkgKQYCLTATCEEDITDLMKNYI-ASYKDMPITIYQMTRKYRDEIRPRGGILRGREFLMKDAYSFASNEEDA 176 baker's yeast
AAH44273  131 eLFRLKDRhnQEYCLGPTHEELVTHLVASEGgINNKELPLLLYQITRKFRDEQRPCFGLLRGREFYMKDMYTFDISEESA 210 African clawed frog
XP_313346 103 eLLQTTDRhgKVQILGPTHEESITSLLAAIApVTYRQFPLRLYQISTKFRDEMKPRFGLMRAKEFLMKDLYTFDVDRAGC 182 Anopheles gambiae ...
AAF47674   99 eFYMVRDRsgKQFLMSPTHEEAVTAMLATTSpISYRQLPLRLFQIGPKFRDELKTRFGLMRAKEFLMKDMYSFDVSEETA 178 fruit fly
NP_689481 140 eLLRLRDRhgKEYCLGPTHEEAITALIASQKkLSYKQLPFLLYQVTRKFRDEPRPRFGLLRGREFYMKDMYTFDSSPEAA 219 human
CAA21147   97 eLFRLHDRndREMCLAPTHEEDVTRTMATII-DSQKQLPIRVYQIGRKFRDELRPRGGLLRGREFMMKDLYTFDIDKASA 175 fission yeast

Feature 1                                                                                     
Q9ZMJ6    171 DKEFLNTQSAYKEILSDlglDFRIVEADSGaiGGSKSREFVVLTEcgedtivvcqncdyaanieiakrskrteplmspsa 250 Helicobacter pylor...
P36431    173 EEQYAKLRLAYQRIFDRlnlKYVIVAADGGkiGKGKSEEFHVLCSlgedticvsgsyganveaaqaippsyvydsnllpv 252 Chlamydia trachomatis
AAO51960  221 EITYYQVKQAYHNILERlelPYACVEADSGniGGNMSHEFQVLANvgedsliyckncnyhaniekakglpstniknlkkq 300 Dictyostelium disc...
P78600    177 MKTYEKVVGAYHKIFQDlgiPYVKAEADSGdiGGSLSHEWHYLNSsgedtvfecnechnvsnmekalsypkeidetievs 256 Candida albicans
P39965    177 FASFQKLDDTYNKIFKDlkiPFVSAWADSGdiGGEFSKEFHLIHEsgedtlmsckhcgdistldmsqsypekdgqysgdv 256 baker's yeast
AAH44273  211 YETYRNVCDAYSNIFRAlgvHFVKVQADTGniGGKMSHEFHLPANigedrllvcgscdfsan------------------ 272 African clawed frog
XP_313346 183 QETYDSVNEAYGRLFREigvPFVKVAGDCGtmGGSLSHEYHFPSEvgedelirctrcgmhtnae---------------- 246 Anopheles gambiae ...
AAF47674  179 METYTLVNAAYDRLFKQlevPFVKVNAATGimGGSVSHEYHYVSPvgednllqcsscgfagn------------------ 240 fruit fly
NP_689481 220 QQTYSLVCDAYCSLFNKlglPFVKVQADVGtiGGTVSHEFQLPVDigedrlaicprcsfsan------------------ 281 human
CAA21147  176 MKTYEDVLQAYHTFFKEvglPFVMVKAATGniGGNLSHEFHYRHPvgedviytcpschystnse---------------- 239 fission yeast

Feature 1                                                                                     
Q9ZMJ6    251 lakfptpnttsapsvaeffktepyfvlkalvnkvihkdketlacffvrgddnleetkalnt------------------- 311 Helicobacter pylor...
P36431    253 eevatpnirtiedlevffntpkhkilktlvvktcqkdsekffaicirgdrqinltkva---------------------- 310 Chlamydia trachomatis
AAO51960  301 ksilnkqinqeinnnntndnqeinikikeieneiknfkniklsimkittlpsnsdkeieeialiinklndnfnqysikpn 380 Dictyostelium disc...
P78600    257 viyfttedkstlicayypsnrvlepkfiqneipdid-------------------------------------------- 292 Candida albicans
P39965    257 dckyaltkdhstlicfyypkdrqlnwnlalnamdkdidltl--------------------------------------- 297 baker's yeast
AAH44273      --------------------------------------------------------------------------------     African clawed frog
XP_313346     --------------------------------------------------------------------------------     Anopheles gambiae ...
AAF47674      --------------------------------------------------------------------------------     fruit fly
NP_689481     --------------------------------------------------------------------------------     human
CAA21147      --------------------------------------------------------------------------------     fission yeast

Feature 1                                                                                     
Q9ZMJ6    312 ------------------lnllganalelreaneedlnkagliagfigpyglkkhvcyiifdedlkegdclivganekdf 373 Helicobacter pylor...
P36431    311 ---------------------sflqvddcelaseeeilkhlhvekgfigplycpipcyadettrpmtnficannqkdvhc 369 Chlamydia trachomatis
AAO51960  381 yndikqtqvlspeeykkyrsiimkgiesknvdnnsdkllsfeeiikhklfidgsvefdeesiykplksllkkllkrdlsr 460 Dictyostelium disc...
P78600    293 -------------------------------------------ldsindlsefnhdistrivrimdsrlssrskfpdfpi 329 Candida albicans
P39965    298 --------------------------------------rnkpndhvlqvyekdnedimfskilrvmdcrlnsksnfpdfp 339 baker's yeast
AAH44273      --------------------------------------------------------------------------------     African clawed frog
XP_313346     --------------------------------------------------------------------------------     Anopheles gambiae ...
AAF47674      --------------------------------------------------------------------------------     fruit fly
NP_689481     --------------------------------------------------------------------------------     human
CAA21147      --------------------------------------------------------------------------------     fission yeast

Feature 1                                           # ## #                                  ##
Q9ZMJ6    374 havgvdlkgfenlvyadivqvkesdccpncqgaLKYHKSLEVGHIFKLGQSYAKsl-kASFLDKng----kERFFEMGCY 448 Helicobacter pylor...
P36431    370 khvnwgrdiplpafgdfllaeagdlcpqnggapYEIFQGVEVAHIFNLGTRYTEsf-sVGFQDKng----dKQLCWMGTY 444 Chlamydia trachomatis
AAO51960  461 kddlilsmnrgqnfreaikgdlcvqpvcdgkslLDTKRGIEVGHIFYLGTKYSSkl-gAYYTGAaq----qRIPLEMGCF 535 Dictyostelium disc...
P78600    330 snfinrslittltdipivlaqegeicghceegkLSASSAIEVGHTFYLGDKYSKpl-dLEVDVPtsnnsieKQRIMMGCY 408 Candida albicans
P39965    340 lkkylknnfgqisdvsivdaqeneicgkceegrLEPLKSIEVGHIFLLGNKYSKplnvKFVDKEnk----nETFVHMGCY 415 baker's yeast
AAH44273  273 ----------------velllhdekncpvctgqLKETKGIEIGHTFYLGTKYSHvf-kANCYDSqk----tPFLAEMGCY 331 African clawed frog
XP_313346 247 ---------------lfpalserckscetgsdtFERQAGIEVAHAFILEDRYTKal-gATCLAAng----kPVPLQMGCY 306 Anopheles gambiae ...
AAF47674  241 ----------------sevvkapascpscnssdLKEVRGVEVAHTFLLGDKYSKpl-gATFLNTtg----kPQSLVMGCY 299 fruit fly
NP_689481 282 ----------------metldlsqmncpacqgpLTKTKGIEVGHTFYLGTKYSSif-nAQFTNVcg----kPTLAEMGCY 340 human
CAA21147  240 --------------mldlsktssdiscpncndqLTSTTAIEVGHAFYLGKIYSSkf-nATVEVKn-----kQEVLHMGCY 299 fission yeast

Feature 1     # #  #        
Q9ZMJ6    449 GIGISRLLSVILEQ 462 Helicobacter pylori J99
P36431    445 GIGVGRTLAACIEQ 458 Chlamydia trachomatis
AAO51960  536 GIGVSRLLATIVET 549 Dictyostelium discoideum
P78600    409 GIGISRIIAAIAEI 422 Candida albicans
P39965    416 GIGVSRLVGAIAEL 429 baker's yeast
AAH44273  332 GIGVSRLLAASLEV 345 African clawed frog
XP_313346 307 GIGVTRLLAASIEV 320 Anopheles gambiae str. PEST
AAF47674  300 GIGITRVIAAALEV 313 fruit fly
NP_689481 341 GLGVTRILAAAIEV 354 human
CAA21147  300 GIGVSRLIAAVAHV 313 fission yeast

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