1LN0,2WSH,1YWL,1YD1,3NIC


Conserved Protein Domain Family
GIY-YIG_SF
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cd00719: GIY-YIG_SF 
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GIY-YIG nuclease domain superfamily
The GIY-YIG nuclease domain superfamily includes a large and diverse group of proteins involved in many cellular processes, such as class I homing GIY-YIG family endonucleases, prokaryotic nucleotide excision repair proteins UvrC and Cho, type II restriction enzymes, the endonuclease/reverse transcriptase of eukaryotic retrotransposable elements, and a family of eukaryotic enzymes that repair stalled replication forks. All of these members contain a conserved GIY-YIG nuclease domain that may serve as a scaffold for the coordination of a divalent metal ion required for catalysis of the phosphodiester bond cleavage. By combining with different specificity, targeting, or other domains, the GIY-YIG nucleases may perform different functions.
Links
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Statistics
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PSSM-Id: 198380
Aligned: 59 rows
Threshold Bit Score: 35.4183
Created: 24-Sep-2002
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 6 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:The last conserved motif (invariant Asn residue) of GIY-YIG homing endonuclease I-TevI (1LN0) and nucleotide excision repair endonucleases UvrC (1YD1) is completely absent in some family members.
  • Structure:1YD1; Thermotoga maritima nucleotide excision repair endonuclease UvrC contains an active site.
  • Comment:1LN0 is a R27A mutant
  • Structure:1LN0; Enterobacteria phage T4 homing endonuclease I-TevI contains a putative active site.
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1          #                    # #               #   #                                  
1LN0_A         4 GIYQIKNtln----------nkVYVGSAkd--------fEKAWKRHFKdlekgchssik--lqrsfnkhgnVFECSiLEE 63  Enterobacteria ...
CBK76336      53 AVYLITDtsn----------gkMYVGSAtaqy----gmlLQRWTNYIDnghggnvelkhivdtkgfdyikaNFQYS-VLE 117 Clostridium cf....
1YD1_A        17 GVYIFKNkg-----------vpIYIGKAkr--------lSNRLRSYLNpqtekv-----------frigeeADELE-TIV 65  Thermotoga mari...
Q8H7F6        88 GVYAVYDksd----------elQFVGISrn--------iAASVSAHLKsvpe----------------lcgSVKVG-IVE 132 thale cress
ABO50363      19 GVYQIKNtkn----------qkVLVIATpnlktmigrkvELRGGGHKNkqlqedwn--------tfgeeafVFEVLeVLE 80  Desulfotomaculu...
EAY56139      27 GIYCVYActhnaqekkvsirklLYIGESen--------vKDRVSGHELkekwe--------------rqlsKGEVL-CFN 83  Leptospirillum ...
CBL01361      26 GVYLVYAgkpvsdk-ivslrklFYIGESsn--------iHDRIVEHNRdkdwk--------------dglkEGELL-YYA 81  Faecalibacteriu...
ZP_05884610   26 GLYLVYTcefavdrntlrllnlIYVGCStd--------iHRAFSQHPLsldfl--------------aelePGQTL-CIA 82  Vibrio corallii...
YP_001798708  64 GIYFVYTldh----------qlLYIGKAdn--------lRTRWNSHHKyqyfi--------------essmDSRIG-YFT 110 Cyanothece sp. ...
ZP_07111669   26 GIYFVVGere----------qlFYIGQAknl------rkRWAGKSHHRykqfa-------------rkgldKIIIK-YIL 75  Oscillatoria sp...

Feature 1                                         #       
1LN0_A        64 IPye----------------------kdLIIERENFWIKEL 82  Enterobacteria phage T4
CBK76336     118 NYnarm-------------------ddnYILSREKWWKDTL 139 Clostridium cf. saccharolyticum K10
1YD1_A        66 VMne-----------------------rEAFILEANLIKKY 83  Thermotoga maritima
Q8H7F6       133 EPdk-----------------------aVLTQAWKLWIEEH 150 thale cress
ABO50363      81 EKeegff-----------------dkadELKKLEKKWLEKL 104 Desulfotomaculum reducens MI-1
EAY56139      84 MAeia--------------------pesARQRAEAAVIYHH 104 Leptospirillum sp. Group II UBA
CBL01361      82 YAlap---------------------kdERLLEEAALIYYY 101 Faecalibacterium prausnitzii SL3/3
ZP_05884610   83 TTpla---------------------pfERHLAKAALAQTF 102 Vibrio coralliilyticus ATCC BAA-450
YP_001798708 111 FDsvdnlnqtieefqseptetiqtkalvTSDQFEKLEQELT 151 Cyanothece sp. ATCC 51142
ZP_07111669   76 ASv------------------------sELNELECKYIKQF 92  Oscillatoria sp. PCC 6506

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