1ECC,1AO0


Conserved Protein Domain Family
GPATase_N
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cd00715: GPATase_N 
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Glutamine amidotransferases class-II (GN-AT)_GPAT- type. This domain is found at the N-terminus of glutamine phosphoribosylpyrophosphate (Prpp) amidotransferase (GPATase) . The glutaminase domain catalyzes amide nitrogen transfer from glutamine to the appropriate substrate. In this process, glutamine is hydrolyzed to glutamic acid and ammonia. GPATase catalyzes the first step in purine biosynthesis, an amide transfer from glutamine to PRPP, resulting in phosphoribosylamine, pyrophosphate and glutamate. GPATase crystalizes as a homotetramer, but can also exist as a homdimer.
Links
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Statistics
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PSSM-Id: 238367
Aligned: 88 rows
Threshold Bit Score: 323.643
Created: 6-Mar-2002
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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active sitetetramer
Conserved site includes 7 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1ECC_B, E.coli GPAT bound to glutamine analog 6-diazo-5-oxo-L-nor-leucine using 3.5A
  • Citation:PMID 9514258
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1       #                            ##                                                #
1ECC_B        1 CGIVGIAGv----MPVNQSIYDALTVLQHRGQDAAGIITIdanncFRLRKANGLVSDVFEAr---hMQRLQGNMGIGHVR 73  Escherichia coli
P41390        2 CGILALMLad-phQQACPEIYEGLYSLQHRGQDAAGIVTAgnkgrLYQCKGSGMVADVFSQh---qLRQLVGSMGIGHLR 77  fission yeast
P04046        2 CGILGIVLan-qtTPVAPELCDGCIFLQHRGQDAAGIATCgsrgrIYQCKGNGMARDVFTQq---rVSGLAGSMGIAHLR 77  baker's yeast
NP_293944    19 CGVFGIYSaq--pNDLAWLTYLGLFALQHRGQEAAGMCVSdg-drFHVEKDLGLVTQVFDErrldsVRLAGAQVSIGHVR 95  Deinococcus radi...
NP_636339     2 CGIVGIVGn----QNVAGQLYDGLTVLQHRGQDAAGIATAdg-trLRVQKANGLVRDVFDEk---kMAVLEGRVGIAHCR 73  Xanthomonas camp...
CAD36991      2 CGILALILadtdaSDASADLHESLYYLQHRGQDACGIATCgsggrIFQCKGNGMASKVFDDgk--rTVDLPGYMGIAHLR 79  Neurospora crassa
XP_323556     2 CGILALILadtdaSDASADLHESLYYLQHRGQDACGIATCgsggrIFQCKGNGMASKVFDDgk--rTVDLPGYMGIAHLR 79  Neurospora crassa
YP_170623     2 CGVIGVAGp----DQVSYALFYGLSLLQHRGQDAAGIATMdh-ghFFIRKNTGLVSDVFTDe---kLEKSKGNMGIGHVR 73  Francisella tula...
ZP_01056130   2 CGILGIASr---qDEVFAELYDGLLMLQHRGQDASGIVTYtg-efFRERKANGLVKDVFNAs---dAETLRGKVGIGHVR 74  Roseobacter sp. ...
XP_762182     2 CGITALLLas-teQSAAPEINEALSLLQHRGQDAAGIVTCgskgrFHQCKANGMVRDVFDVn---aIARLQGSMGVGHVR 77  Ustilago maydis 521

Feature 1                                   ##                        #                         
1ECC_B       74 YPTAGSSsasEAQPFYVNsp-yGITLAHNGNLTNAHELRKKLfeekrrhiNTTSDSEILLNIFASELdnfrhy---plea 149 Escherichia coli
P41390       78 YPTAGSCahsEAQPFYVNsp-yGLVLGHNGNLINGPELRRFLdteahrhvNTGSDSELLLNIFAYELqrldkf---rine 153 fission yeast
P04046       78 YPTAGSSansEAQPFYVNsp-yGINLAHNGNLVNTASLKRYMdedvhrhiNTDSDSELLLNIFAAELekhnky---rvnn 153 baker's yeast
NP_293944    96 YSTTGSNlrfNAQPLTTRtnkgILGMAHNGNFVNALEVRREMleeg-alfATTNDSEVMLNLIARESrm----------- 163 Deinococcus radi...
NP_636339    74 YPTAGSEgmdEAQPFYVNsp-yGIALAHNGNLINTEALRQQVfeadrrniNTDSDSEVLLNVFAYELdaqrm-----ltp 147 Xanthomonas camp...
CAD36991     80 YPTAGTSssaESQPFYVNsp-yGICFAHNGNLINAPALRSYLdqeahrhvNTDSDSELMLNVFANALnetgka---rvnt 155 Neurospora crassa
XP_323556    80 YPTAGTSssaESQPFYVNsp-yGICFAHNGNLINAPALRSYLdqeahrhvNTDSDSELMLNVFANALnetgka---rvnt 155 Neurospora crassa
YP_170623    74 YPTAGSLgaaDSQPFYVNnp-hGIVFVHNGNLTNVPELAQMLhdierrhlNTSSDSELLLNFFACGMnkskgs----ats 148 Francisella tula...
ZP_01056130  75 YPTAGSLsasEAQPFFVNap-yGIYLVHNGNITNTAEQRDKVtakysrhlRTTSDSEILLNVLADKIsdsikvngtvdpi 153 Roseobacter sp. ...
XP_762182    78 YPTAGSSahsEAQPFYVNsp-yGITFAHNGNLINTDELRRFLdadahrhiNTDSDSEVLLNIFANNLqktgkf---rine 153 Ustilago maydis 521

Feature 1                                                                                       
1ECC_B      150 dnIFAAIAATNRLIRGAYACVAMIIgHGMVAFRDPNGIRPLVLGKrdid--------enrtEYMVASESVALDtlGFDFL 221 Escherichia coli
P41390      154 ndIFEALRNVYDRVNGGYACVAMIAgLGVLGFRDPNGIRPLVIGErdtp---------egkDYMLASESVVLTqfGYRTF 224 fission yeast
P04046      154 edVFHALEGVYRLCRGGYACVGLLAgFALFGFRDPNGIRPLLFGErenp--------dgtkDYMLASESVVFKahNFTKY 225 baker's yeast
NP_293944   164 -dLVEATAAAMQRLRGGYACVLMSR-SQLLGFRDPHGVRPLVIGQre------------dgAWVLASEPCALYaiGARLI 229 Deinococcus radi...
NP_636339   148 eaAIRAVAGVHRRCKGGYAVVSVVLgLGLVAFRDPHGIRPLVLGKreha---------egtEYIVSSESAALDilGYQRV 218 Xanthomonas camp...
CAD36991    156 ddIFSALKKTYERCQGGWAVTAMIAgFGIFAFRDQYGIRPLIMGSrpsst------leggiDYMFASESIALRqlGFSNF 229 Neurospora crassa
XP_323556   156 ddIFSALKKTYERCQGGWAVTAMIAgFGIFAFRDQYGIRPLIMGSrpsst------leggiDYMFASESIALRqlGFSNF 229 Neurospora crassa
YP_170623   149 eaVYKACKFVFEHVKGGYACTAMIAnFGLIAFRDPYGIRPLVLGFkeyd--------dgekAYMVASESVALDisGFKVL 220 Francisella tula...
ZP_01056130 154 rnLFAGVKMTMERIQGAYSVICMVAgVGMLAFRDPHGIRPLSVARreae--------gdgdDYAFASEDVAFGinGFEKL 225 Roseobacter sp. ...
XP_762182   154 edIFTAIRDLMKQCKGGYTCAAMIAgFGIIAFRDPNGIRPLGMCSrpssatnteyleghakDYLFTSESVVCDalGFEDF 233 Ustilago maydis 521

Feature 1                                                                
1ECC_B      222 RDVAPGEAIYITEEGqlftr-----------qcadNPVSNPCLFEYVYFARPDSFID 267 Escherichia coli
P41390      225 RDIRPGECVFIRRSNredilagfrgprlfsrqilpCLRFTPDIFEYVYFARPDSVID 281 fission yeast
P04046      226 RDLKPGEAVIIPKNCskgepe--------fkqvvpINSYRPDLFEYVYFARPDSVLD 274 baker's yeast
NP_293944   230 RDVAPGELVWIDREGlhsl-------------mveARQPTPCSFEWIYFARSDSQLD 273 Deinococcus radiodurans
NP_636339   219 RDVRPGEALVITARGelfse-----------icaaPTDHAPCIFEYVYFARPDSMID 264 Xanthomonas campestris pv. campestris s...
CAD36991    230 QDILPGQAVFIQKNGqpqfh-----------qvveQKAYSPDIFEYVYFARPDTIMD 275 Neurospora crassa
XP_323556   230 QDILPGQAVFIQKNGqpqfh-----------qvveQKAYSPDIFEYVYFARPDTIMD 275 Neurospora crassa
YP_170623   221 RDVEPGEVIIITEDRkvhsk-----------icakNPVLAPCLFEYVYFARPDSIMN 266 Francisella tularensis subsp. tularensi...
ZP_01056130 226 RDVNPGEAILIDLDGnmhs------------fqavEGTLTPCIFEYVYLARPDSMLD 270 Roseobacter sp. MED193
XP_762182   234 EDIKAGEAVIITRQAvsrrilt------ppadsddAFAFSPDIFEYVYFARPDSVMD 284 Ustilago maydis 521

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