1DGP,1DI1,1PS1


Conserved Protein Domain Family
Terpene_cyclase_nonplant_C1

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cd00687: Terpene_cyclase_nonplant_C1 
Click on image for an interactive view with Cn3D
Non-plant Terpene Cyclases, Class 1
This CD includes terpenoid cyclases such as pentalenene synthase and aristolochene synthase which, using an all-trans pathway, catalyze the ionization of farnesyl diphosphate, followed by the formation of a macrocyclic intermediate by bond formation between C1 with either C10 (aristolochene synthase) or C11 (pentalenene synthase), resulting in production of tricyclic hydrocarbon pentalenene or bicyclic hydrocarbon aristolochene. As with other enzymes with the 'terpenoid synthase fold', they have two conserved metal binding motifs, proposed to coordinate Mg2+ ion-bridged binding of the diphosphate moiety of FPP to the enzymes. Metal-triggered substrate ionization initiates catalysis, and the alpha-barrel active site serves as a template to channel and stabilize the conformations of reactive carbocation intermediates through a complex cyclization cascade. These enzymes function in the monomeric form and are found in fungi, bacteria and Dictyostelium.
Statistics
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PSSM-Id: 173835
View PSSM: cd00687
Aligned: 14 rows
Threshold Bit Score: 281.175
Threshold Setting Gi: 13365759
Created: 6-Mar-2002
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 20 residues -Click on image for an interactive view with Cn3D
Feature 1:substrate binding pocket [chemical binding site]
Evidence:
  • Comment:serves as a template to channel and stabilize the conformations of reactive carbocation intermediates through a complex cyclization cascade
  • Structure:1DGP; Penicillium roquefortii Aristolochene synthase-Farnesol complex
    View structure with Cn3D
  • Citation:PMID 9295272

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                      #   #                #  ##  ##   
1DGP_A        4 PTQWSYLCHPRVKEVQDEVDGYFLen-wkFPSFKAVRTFLda-kFSEVTCLYFPLa-lDDRIHFACRLLTVLFLIDDVLE 80  Penicillium roqu...
1DI1_A        4 PTQWSYLCHPRVKEVQDEVDGYFLen-wkFPSFKAVRTFLda-kFSEVTCLYFPLa-lDDRIHFACRLLTVLFLIDDVLE 80  Penicillium roqu...
1PS1_A        8 HIPLPGRQSPDHARAEAEQLAWPRsl-glIRSDAAAERHLrg-gYADLASRFYPHa-tGADLDLGVDLMSWFFLFDDLFD 84  Streptomyces sp....
AAO52111     19 PSEWSYPINKNYKHIKDTYNEGVSc---gIFEASNEKHFAyangVLNCVTWFYPKy-dYEQCMVAASIMLWIFVLDDFLE 94  Dictyostelium di...
AAF13263     18 PSTFQPLCHPLVEEVSKEVDGYFLqh-wnFPNEKARKKFVaa-gFSRVTCLYFPKa-lDDRIHFACRLLTVLFLIDDLLE 94  Aspergillus terreus
ZP_00085244  57 CPPPTRIDEALGQEVNERLMEWISni--gIFAGKEEKIRAs--dFGRYAMLCHADtnnPDRLLLVAQCFAALFAVDDHYC 132 Pseudomonas fluo...
CAB94607     27 EAAFPRQLHPYWPKLQETTRTWLLek-rlMPADKVEEYADgl-cYTDLMAGYYLGa-pDEVLQAIADYSAWFFVWDDRHD 103 Streptomyces coe...
CAB41566    382 RMPYPLELSPALDGARRHLSEWCRem-giLSEGVWDEDKLescdLPLCAAGLDPDa-tQDQLDLASGWLAFGTYGDDYYP 459 Streptomyces coe...
BAB20433    124 HHPVPEPDPVRVEEVSRRIKRWAEde-vqLYPEEWEGQFDgf-sVGRYMVGCHPDaptVDHLMLATRLMVAENAVDDCYC 201 Streptomyces coe...
NP_630182    12 YLPHPARLNPHLDEARAHSTTWARemgmlEGSGVWEQSDLeahdYGLLCAYTHPDc-dGPALSLITDWYVWVFFFDDHFL 90  Streptomyces coe...
Feature 1                                                                                       
1DGP_A       81 Hm-----sFADGEAYNNRLIPISRGdvlp--------drtkPEEFILYDLWESMRAhd---------aeLANEVLEPTFV 138 Penicillium roqu...
1DI1_A       81 Hm-----sFADGEAYNNRLIPISRGdvlp--------drtkPEEFILYDLWESMRAhd---------aeLANEVLEPTFV 138 Penicillium roqu...
1PS1_A       85 Gprg--enPEDTKQLTDQVAAALDGplpd---------tapPIAHGFADIWRRTCEgmt--------paWCARSARHWRN 145 Streptomyces sp....
AAO52111     95 Rdh---mtDEKQQYCVKKYEDILIFgkdssyisnieyndltPLDKYSLILRKRLLNptih------rieSFNIFIHYLRE 165 Dictyostelium di...
AAF13263     95 Ym-----sFEEGSAYNEKLIPISRGdvlp--------drsiPVEYIIYDLWESMRAhd---------reMADEILEPVFL 152 Aspergillus terreus
ZP_00085244 133 Ddq---slGGRPETVAESLSFALTAidpvylp---spfdkeLLKQQMCDPVIRGLLaymkrvaqfctpsQVARVRQITIA 206 Pseudomonas fluo...
CAB94607    104 Rd------IVHGRAGAWRRLRGLLHtaldspg--dhlhhedTLVAGFADSVRRLYAflp--------atWNARFARHFHT 167 Streptomyces coe...
CAB41566    460 LvyghrrdLAAARLTTTRLSDCMPLdgepv------pppgnAMERSLIDLWVRTTAgmt--------peERRPLKKAVDD 525 Streptomyces coe...
BAB20433    202 Ed-----hGGSPVGLGGRLLLAHTAidhfhst---aeytptWQASLAADAPRRAYDsamgyfvraatpsQSDRYRHDMAR 273 Streptomyces coe...
NP_630182    91 Eky---krSQDRLAGKAHLDRLPLFmplddaa--gmpeprnPVEAGLADLWTRTVPams--------adWRRRFAVATEH 157 Streptomyces coe...
Feature 1       #          #           #   #    ##  #  #                                 ##  #  
1DGP_A      139 FMRAQTDR--ARLSIH---ELGHYLEYREKDvGKALLSALMRFSMglrLSADELQD--MKALEANCAKQLSVVNDIYSYD 211 Penicillium roqu...
1DI1_A      139 FMRAQTDR--ARLSIH---ELGHYLEYREKDvGKALLSALMRFSMglrLSADELQD--MKALEANCAKQLSVVNDIYSYD 211 Penicillium roqu...
1PS1_A      146 YFDGYVDEaeSRFWNApcdSAAQYLAMRRHTiGVQPTVDLAERAGrfeVPHRVFDSavMSAMLQIAVDVNLLLNDIASLE 225 Streptomyces sp....
AAO52111    166 WFFSIIPLkkSKGNNHdsvPIEVYQFIRTINiGLFFVVGVNSVAVrtnVHGSFWLNpiWNRMVRSASRQIIIFNDCVSYA 245 Dictyostelium di...
AAF13263    153 FMRAQTDR--TRARPM---GLGGYLEYRERDvGKELLAALMRFSMglkLSPSELQR--VREIDANCSKHLSVVNDIYSYE 225 Aspergillus terreus
ZP_00085244 207 MFVTMAAEgpWRLYGTq-pTVAEYLASRQVN-SFWPCLVLIDLIGgyeVPANTYSRpdIHHVTALASLATTLVNDLYSAY 284 Pseudomonas fluo...
CAB94607    168 VIEAYDREfhNRTRGIv-pGVEEYLELRRLTfAHWIWTDLLEPSSgceLPDAVRKHpaYRRAALLSQEFAAWYNDLCSLP 246 Streptomyces coe...
CAB41566    526 MTEAWLWElsNQIQNRv-pDPVDYLEMRRATfGSDLTLGLCRAGHgpaVPPEVYRSgpVRSLENAAIDYACLLNDVFSYQ 604 Streptomyces coe...
BAB20433    274 LHLGYLAEgaWAQTGHv-pEVWEYLAMRQFN-NFRPCPTITDTVGgyeLPADLHARpdMQRVIALAGNATTIVNDLYSYT 351 Streptomyces coe...
NP_630182   158 LLNESMWElsNINEGRv-aNPVEYIEMRRKVgGAPWSAGLVEYATa-eVPAAVAGTrpLRVLMETFSDAVHLRNDLFSYQ 235 Streptomyces coe...
Feature 1       #                                                                               
1DGP_A      212 KEE--EASRtghkegaflCSAVKVLAEESKLGIPATKRVLWSMTREWETVHDEIVAEKiaspd----------gcseaAK 279 Penicillium roqu...
1DI1_A      212 KEE--EASRtghkegaflCSAVKVLAEESKLGIPATKRVLWSMTREWETVHDEIVAEKiaspd----------gcseaAK 279 Penicillium roqu...
1PS1_A      226 KEEa-RGEQ---------NNMVMILRREHGWSKSRSVSHMQNEVRARLEQYLLLESCLpkvgeiyq----ldtaereaLE 291 Streptomyces sp....
AAO52111    246 KELn-HDCAg--------ENCLYILQKKLNLPFEKVYSELIAEAKQCIFDIQNDEKLLikslshi------seeemngVI 310 Dictyostelium di...
AAF13263    226 KEL--YTSKtahseggilCTSVQILAQEADVTAEAAKRVLFVMCREWELRHQLLVARLsaegl-----------etpgLA 292 Aspergillus terreus
ZP_00085244 285 KEHlnETGD---------FKLPYLLAARHNCSLQEAIDLAADIHDAVMEEYERLHATLmkgt------------rspvLR 343 Pseudomonas fluo...
CAB94607    247 KEI--AGDEv--------HNLGISLITHHSLTLEEAIGEVRRRVEECITEFLAVERDAlrfadeladgtvrgkelsgaVR 316 Streptomyces coe...
CAB41566    605 KEIe-YEGEi--------HNAVLVVQNFFGVDYPAALGVVQDLMNQRMRQFEHVVAHElpvvyddfq---lseeartvMR 672 Streptomyces coe...
BAB20433    352 KELn-SPGRh--------LNLPVVIAEREQLCERDAYLKAVEVHNELQHSFEAAAADLaeac------------plppVL 410 Streptomyces coe...
NP_630182   236 REVe-DEGEl--------SNGVLVLETFFGCTTQEAADLVNDVLTSRLHQFEHTAFTEvpavalekg---ltplevaaVG 303 Streptomyces coe...
Feature 1                       #     
1DGP_A      280 AYMK-GLEYQMSGNEQWSKTTR 300 Penicillium roquefortii
1DI1_A      280 AYMK-GLEYQMSGNEQWSKTTR 300 Penicillium roquefortii
1PS1_A      292 RYRTdAVRTVIRGSYDWHRSSG 313 Streptomyces sp. UC5319
AAO52111    311 YLVE-QLHEIICGNRDWALMTP 331 Dictyostelium discoideum
AAF13263    293 AYVE-GLEYQMSGNELWSQTTL 313 Aspergillus terreus
ZP_00085244 344 RYLT-GLSTWIGGNLEWHKHSA 364 Pseudomonas fluorescens PfO-1
CAB94607    317 ANVG-NMRNWFSSVYWFHHESG 337 Streptomyces coelicolor A3(2)
CAB41566    673 GYVT-DLQNWMAGILNWHRNVP 693 Streptomyces coelicolor A3(2)
BAB20433    411 RFLR-GVAAWVDGNHDWHRTNT 431 Streptomyces coelicolor
NP_630182   304 AYTK-GLQDWQSGGHEWHMRSS 324 Streptomyces coelicolor A3(2)

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