1EZF


Conserved Protein Domain Family
Trans_IPPS_HH

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cd00683: Trans_IPPS_HH 
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Trans-Isoprenyl Diphosphate Synthases, head-to-head
These trans-Isoprenyl Diphosphate Synthases (Trans_IPPS) catalyze a head-to-head (HH) (1'-1) condensation reaction. This CD includes squalene and phytoene synthases which catalyze the 1'-1 condensation of two 15-carbon (farnesyl) and 20-carbon (geranylgeranyl) isoprenyl diphosphates, respectively. The catalytic site consists of a large central cavity formed by mostly antiparallel alpha helices with two aspartate-rich regions (DXXXD) located on opposite walls. These residues mediate binding of prenyl phosphates. A two-step reaction has been proposed for squalene synthase (farnesyl-diphosphate farnesyltransferase) in which, two molecules of FPP react to form a stable cyclopropylcarbinyl diphosphate intermediate, and then the intermediate undergoes heterolysis, isomerization, and reduction with NADPH to form squalene, a precursor of cholestrol. The carotenoid biosynthesis enzyme, phytoene synthase (CrtB), catalyzes the condensation reaction of two molecules of geranylgeranyl diphosphate to produce phytoene, a precursor of beta-carotene. These enzymes produce the triterpene and tetraterpene precursors for many diverse sterol and carotenoid end products and are widely distributed among eukareya, bacteria, and archaea.
Statistics
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PSSM-Id: 173831
View PSSM: cd00683
Aligned: 78 rows
Threshold Bit Score: 171.264
Threshold Setting Gi: 11362526
Created: 6-Mar-2002
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 18 residues -Click on image for an interactive view with Cn3D
Feature 1:substrate binding pocket [chemical binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                  # #                  #   #  #   #                                    
1EZF_A       11 KTCYKYLnqTSRSFAAVIQALDGEMRNAVCIFYLVLRALDTLEDDMtisvekKVPLLHNFHSFLYQpdwrfmesk----- 85  human
P36596       36 RRCYQLLdmTSRSFAVVIKELPNGIREAVMIFYLVLRGLDTVEDDMtlpldkKLPILRDFYKTIEVegwtfnesgp---- 111 fission yeast
P29704       40 LHCFELLnlTSRSFAAVIRELHPELRNCVTLFYLILRALDTIEDDMsiehdlKIDLLRHFHEKLLLtkwsfdgnap---- 115 baker's yeast
Q92459       48 RDCWGFLdlTSRSFAAVIKELKGDVSRVICLFYLVLRALDTVEDDMtipaqrKIPLLVNFYKYLEQpgwnftesgp---- 123 Ustilago maydis
AAD22408     40 RRCYELLdvCSRSFAAVIRELHPEVRDAVMLFYLILRALDTIEDDMtlsrdiKIPILRDFTKCMKTpgwkftdsdp---- 115 Yarrowia lipolytica
T45141       19 EFQAHFLdgVSRTFALTIPRLPEGLARPVSNGYLLCRIVDTIEDEValtstqKRRYCEHFARVVAGtapaapladelfpl 98  Methylococcus ca...
NP_279693    58 EWCHSAVqdVSRTFALTIDALEEPMATRICVGYLLCRVADTVEDATamppteQAQLLETYDAALDPdcgttvaefqaavg 137 Halobacterium sp...
NP_681887    25 CFCQEILpqVSRTFALSIRFLPGNLGRAVLVAYLICRIADTVEDDPvasiaaKTALLDHLLQCFDSpalansygetarg- 103 Thermosynechococ...
ZP_00003596  15 RYQEHILqgVSRTFALTIPQLPPALREVVGNAYLLCRIVDTVEDDSaltleqTRELAEMFIEVVSGtveaetfaralypl 94  Nitrosomonas eur...
ZP_00089878  19 AYQNAILskVSRTFALTIPQLPPLLRRAVTNAYLLCRIADTIEDEPafsaeeKRRYEDAFIDAVTGriapqyfstelasr 98  Azotobacter vine...
Feature 1                                                                              #    #   
1EZF_A       86 -------ekdrqvleDFPTISLEFRNLAekyQTVIADICRRMGIGMAEFLd-------KHVTSe--QEWDKYCHYVAGLV 149 human
P36596      112 ------nekdrqllvEFDVVIKEYLNLSegyRNVISNITKEMGDGMAYYAslaekndgFSVETi--EDFNKYCHYVAGLV 183 fission yeast
P29704      116 ------dvkdravltDFESILIEFHKLKpeyQEVIKEITEKMGNGMADYIlden-ynlNGLQTv--HDYDVYCHYVAGLV 186 baker's yeast
Q92459      124 ------nekdrqllvEFDKVIAEYQLLDvgyKTVISDITAKMGAGMASYIelsa-kgpLKVAMw--KHFDLYCHFVAGLV 194 Ustilago maydis
AAD22408    116 ------nerdrvvlqEFPVVMTEFNKLKpkyQEVIYDITDRMGNGMADYViddd-fnnNGVDTi--AAYDLYCHHVAGIV 186 Yarrowia lipolytica
T45141       99 -lsdqtlaaereliaAIPRVISITHGFAapqQEALAECVATMSRGMAEFQdkd---lrHGLEDl--RQMGDYCYYVAGVV 172 Methylococcus ca...
NP_279693   138 dhvpsepnadwrvvaNTTRVVSAFESLDdaaKAAIRPTVREMATGMASFVqry--adaGGLRIqspTELEEYCWYVAGTV 215 Halobacterium sp...
NP_681887   104 ---vqgepahvqlvkHTGIVFTLYRSLPrtsQQHVQRWVSEMVHGMKKFInly--pngIRIQTl--AEYKEYCYYVAGTV 176 Thermosynechococ...
ZP_00003596  95 -lsehtipaehdlikHTPSVIRMTHSFNpvqRQALERCIRIMGQGMAAYQese---slSGLENl--AEMDRYCYHVAGVV 168 Nitrosomonas eur...
ZP_00089878  99 -fstetseaerdlvsQLPLVLQVTNSLKpaqRMAIVNCLKVMSHGMHDFQrnv---gqHGLETl--CDMDCYCYCVAGVV 172 Azotobacter vine...
Feature 1       #                             #  #      ##  ##     #                            
1EZF_A      150 GIGLSRLFSasef--edplVGEDTERANSMGLFLQKTNIIRDYLEDQ-QGGREFWPQEVWSRYVkklgdfa--------- 217 human
P36596      184 GIGLSRLFAqskl--edpdLAHSQAISNSLGLFLQKVNIIRDYREDF-DDNRHFWPREIWSKYTssfgdlc--------- 251 fission yeast
P29704      187 GDGLTRLIViakf--anesLYSNEQLYESMGLFLQKTNIIRDYNEDL-VDGRSFWPKEIWSQYApqlkdfm--------- 254 baker's yeast
Q92459      195 GEGLSRLFSeskl--erpwLGHQLELSNHMGLFLQKTNIIRDYAEDC-EEGRYFWPQQCWGDDFakfesqpdvakgiiei 271 Ustilago maydis
AAD22408    187 GEGLTRITIlagf--gtdvLHENPRLQESMGLFLQKVNIIRDYREDI-DVNRAFWPREIWHKYAeemrdfk--------- 254 Yarrowia lipolytica
T45141      173 GEMLTRLFChyspe-iaahRSRLMELACPFGQGLQMTNILKDLWDDH-ARGVCWLPQEVFTECGfsltelr--------- 241 Methylococcus ca...
NP_279693   216 GELVTELLArdap---sdaAAAMRENARSFALLLQLVNVAKDVRPDYeEENNVYLPQEWLDDHDlapedva--------- 283 Halobacterium sp...
NP_681887   177 GYLLTDLWHehspsigadeYQVLLKRCAAFGEALQTVNILKDIAWDAeHENSIYIPNESLILQGsshqtll--------- 247 Thermosynechococ...
ZP_00003596 169 GEMLTELFCdyspe-inqhKPALMQLSVSFGQGLQMTNILKDIWEDR-KRGACWLPRDIFLQEGfdlndlq--------- 237 Nitrosomonas eur...
ZP_00089878 173 GEMLTELLIdfdpa-lasqRDPLMRLAISFGQGLQMTNILKDQWEDY-RRGVCWLPQDVFARYGvrleelq--------- 241 Azotobacter vine...
Feature 1                                                         ##                            
1EZF_A      218 --------kpenidlAVQCLNELITNALHHIPDVITYlsrlrn--qsvfnFCAIPQVMAIATLAa-CYNNQQVFKg---a 283 human
P36596      252 --------lpdnsekALECLSDMTANALTHATDALVYlsqlkt--qeifnFCAIPQVMAIATLAa-VFRNPDVFQt---n 317 fission yeast
P29704      255 --------kpeneqlGLDCINHLVLNALSHVIDVLTYlagihe--qstfqFCAIPQVMAIATLAl-VFNNREVLHg---n 320 baker's yeast
Q92459      272 kpghfrpadnelgqrSMYVLSSMLLDAMSHATHALDYlallke--qsvfnFCAIPQVMAIATLEl-MFNNPDVFKk---n 345 Ustilago maydis
AAD22408    255 --------dpkyskkALHCTSDLVANALGHATDCLDYldnvtd--pstftFCAIPQVMAIATLDl-VYRNPDVFQk---n 320 Yarrowia lipolytica
T45141      242 --------phhanpdFVRGFERLIGVAHAHLRNALEYtlliprhetgireFCLWALGMAVLTLR--KIHRHPYFSds-aq 310 Methylococcus ca...
NP_279693   284 --------dpehagrVASVVERVTDRARGYVADAQRWlevmpt--sqgntLAAWAVPFLLAVGTmrELGNRPADVvregd 353 Halobacterium sp...
NP_681887   248 --------saehlqqNHAAIKELIALAWHDLDEAQAYllsvpkaaipirlFCVLPLLFAYATLRe-LTHSTAMLQpg-gg 317 Thermosynechococ...
ZP_00003596 238 --------pgcpergFHAGLGTLIGIAKTHLQNALAYtclipsheagvrrFCLWAIGMAVLTLN--KINHKRDFItg-re 306 Nitrosomonas eur...
ZP_00089878 242 --------agrqdanYMSALTELIGVAHAHLRDALEYtlmipnrhsgfrrFCLWSIGLAVLTLR--KLQQNPHFSag-eq 310 Azotobacter vine...
Feature 1                 
1EZF_A      284 VKIRKGQAVT 293 human
P36596      318 VKIRKGQAVQ 327 fission yeast
P29704      321 VKIRKGTTCY 330 baker's yeast
Q92459      346 VKIRKGVAVG 355 Ustilago maydis
AAD22408    321 VKLRKGTTVS 330 Yarrowia lipolytica
T45141      311 VKITRQAVKA 320 Methylococcus capsulatus
NP_279693   354 VKVDREEVHA 363 Halobacterium sp. NRC-1
NP_681887   318 VKISRAEVKS 327 Thermosynechococcus elongatus BP-1
ZP_00003596 307 VKISRRSVRA 316 Nitrosomonas europaea
ZP_00089878 311 VKVSRKAVAY 320 Azotobacter vinelandii

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