1EG9,2B1X,2B24,1ULJ,3EN1,2DE7,2DE7,3GCF,1Z03,3GKE,3GB4,3GOB,3GTE


Conserved Protein Domain Family
RHO_alpha_C

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cd00680: RHO_alpha_C 
Click on image for an interactive view with Cn3D
C-terminal catalytic domain of the oxygenase alpha subunit of Rieske-type non-heme iron aromatic ring-hydroxylating oxygenases
C-terminal catalytic domain of the oxygenase alpha subunit of Rieske-type non-heme iron aromatic ring-hydroxylating oxygenase (RHO) family. RHOs, also known as aromatic ring hydroxylating dioxygenases, utilize non-heme Fe(II) to catalyze the addition of hydroxyl groups to the aromatic ring, an initial step in the oxidative degradation of aromatic compounds. RHOs are composed of either two or three protein components, and are comprised of an electron transport chain (ETC), and an oxygenase. The ETC transfers reducing equivalents from the electron donor to the oxygenase component, which in turn transfers electrons to the oxygen molecules. The oxygenase components are oligomers, either (alpha)n or (alpha)n(beta)n. The alpha subunits are the catalytic components and have an N-terminal domain, which binds a Rieske-like 2Fe-2S cluster, and a C-terminal domain which binds the non-heme Fe(II). The Fe(II) is co-ordinated by conserved His and Asp residues. Oxygenases belonging to this family include the alpha subunits of Pseudomonas resinovorans strain CA10 anthranilate 1,2-dioxygenase, Stenotrophomonas maltophilia dicamba O-demethylase, Ralstonia sp. U2 salicylate-5-hydroxylase, Cycloclasticus sp. strain A5 polycyclic aromatic hydrocarbon dioxygenase, toluene 2,3-dioxygenase from Pseudomonas putida F1, dioxin dioxygenase of Sphingomonas sp. Strain RW1, plant choline monooxygenase, and the polycyclic aromatic hydrocarbon (PAH)-degrading ring-hydroxylating dioxygenase from Sphingomonas CHY-1. This group also includes the C-terminal catalytic domains of MupW, part of the mupirocin biosynthetic gene cluster in Pseudomonas fluorescens, and Pseudomonas aeruginosa GbcA (glycine betaine catabolism A). This family belongs to the SRPBCC (START/RHO_alpha_C/PITP/Bet_v1/CoxG/CalC) domain superfamily of proteins that bind hydrophobic ligands. SRPBCC domains have a deep hydrophobic ligand-binding pocket.
Statistics
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PSSM-Id: 176852
View PSSM: cd00680
Aligned: 98 rows
Threshold Bit Score: 56.4204
Threshold Setting Gi: 254574850
Created: 24-Sep-2002
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 18 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:based on substrate and Fe(II) binding sites of various RHO_alpha_C domains
  • Structure:1EG9_A; Pseudomonas putida oxygenase component of naphthalene 1,2-dioxygenase binds indole (contacts at 4A) and Fe (II).
    View structure with Cn3D
  • Structure:3EN1_A; Pseudomonas putida oxygenase component of toluene 2,3-dioxygenase binds toluene (contacts at 4A) and Fe(II).
    View structure with Cn3D
  • Structure:2DE7_C; Janthinobacterium oxygenase component of carbazole 1,9a-dioxygenase binds carbazole (contacts at 4A) and Fe (II).
    View structure with Cn3D
  • Structure:1Z03_A; Pseudomonas putida oxygenase component of 2-oxoquinoline 8-monooxygenase binds 2-oxoquinoline (contacts at 4A) and Fe(II).
    View structure with Cn3D
  • Structure:3GOB_A; Stenotrophomonas maltophilia oxygenase component of dicamba monooxygenase binds 3,6-dichlorosalicylic acid (the reaction product, contacts at 4A) and Co(II).
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                     ##  ## ##   #                                                   
1EG9_A    185 PPGKVVIKANWKAPAENFVgDAYHVgWTHASslrsgesifsslagnaalppegaGLQMTSky------------------ 246 Pseudomonas putida
2DE7_C    161 LGKNQIIKSNWRLAVENGF-DPSHI-YIHKDsilvkdndlal-plgfapggdrkQQTRVVdddv---------------- 221 Janthinobacterium
2DE7_A    161 LGKNQIIKSNWRLAVENGF-DPSHI-YIHKDsilvkdndlal-plgfapggdrkQQTRVVdddv---------------- 221 Janthinobacterium
3GCF_A    167 LGIRRTVQSNWRLGVENGF-DTTHI-FMHRNspwvsgnrla---fpygfvpadrDAMQVYden----------------- 224 Nocardioides aroma...
1Z03_A    199 HGMHRTGFGNWRIACENGF-DNAHI-LVHKDntivhamdwv---lplgllptsdDCIAVVeddd---------------- 257 Pseudomonas putida
3GKE_A    138 VGGYGHVDCNYKLLVDNLM-DLGHAqYVHRAnaqtda------------fdrleREVIVGd------------------- 185 Stenotrophomonas m...
3GB4_A    138 VGGYGHVDCNYKLLVDNLM-DLGHAqYVHRAnaqtda------------fdrleREVIVGd------------------- 185 Stenotrophomonas m...
3GOB_A    138 VGGYGHVDCNYKLLVDNLM-DLGHAqYVHRAnaqtda------------fdrleREVIVGd------------------- 185 Stenotrophomonas m...
3GTE_A    138 VGGYGHVDCNYKLLVDNLM-DLGHAqYVHRAnaqtda------------fdrleREVIVGd------------------- 185 Stenotrophomonas m...
BAC65426  194 GDQRQYVHGNWKLYAENTR-DPYHAsLLHLFhntfglyr-------stqtgkslMDSEKRhsllysiaasnddaadkqay 265 Sphingomonas sp. P2
Feature 1                                                               ## #       # #        
1EG9_A    247 -----------gSGMGVLWDgysgvhsadlvpelmafggakqerlnkeigdvrariyrSHLNCTVFPNNSMLTc------ 309 Pseudomonas putida
2DE7_C    222 --------vgrkGVYDLIGEhgvpvfegtigge-------------vvregaygekivANDISIWLPGVLKVNpfpn--- 277 Janthinobacterium
2DE7_A    222 --------vgrkGVYDLIGEhgvpvfegtigge-------------vvregaygekivANDISIWLPGVLKVNpfpn--- 277 Janthinobacterium
3GCF_A    225 ---------wpkGVLDRLSEnympvfeatldget-----------vlsaeltgeekkvAAQVSVWLPGVLKVDpfpd--- 281 Nocardioides aroma...
1Z03_A    258 ---------gpkGMMQWLFTdkwapvlenqe----------------lglkveglkgrHYRTSVVLPGVLMVEnwpe--- 309 Pseudomonas putida
3GKE_A    186 -----------gEIQALMKIpggtpsvlmak----------------flrgantpvdaWNDIRWNKVSAMLNFiavapeg 238 Stenotrophomonas m...
3GB4_A    186 -----------gEIQALMKIpggtpsvlmak----------------flrgantpvdaWNDIRWNKVSAMLNFiavapeg 238 Stenotrophomonas m...
3GOB_A    186 -----------gEIQALMKIpggtpsvlmak----------------flrgantpvdaWNDIRWNKVSAMLNFiavapeg 238 Stenotrophomonas m...
3GTE_A    186 -----------gEIQALMKIpggtpsvlmak----------------flrgantpvdaWNDIRWNKVSAMLNFiavapeg 238 Stenotrophomonas m...
BAC65426  266 gdsrtfdtefklQDMSLLKGrqef------------------------------ddgiTLVILAVFPNLVLQQiq----- 310 Sphingomonas sp. P2
Feature 1               # #                                    #         ##   #               
1EG9_A    310 --------sGVFKVWNPIDANTTEVWTYAIVEkdmpe---dlkrrLADSVQRtfgpaGFWE-SDDNDNMETASQNgkkyq 377 Pseudomonas putida
2DE7_C    278 ------pdmMQFEWYVPIDENTHYYFQTLGKPcandeerkkyeqeFESKWKPma--lEGFN-NDDIWAREAMVDFyadd- 347 Janthinobacterium
2DE7_A    278 ------pdmMQFEWYVPIDENTHYYFQTLGKPcandeerkkyeqeFESKWKPma--lEGFN-NDDIWAREAMVDFyadd- 347 Janthinobacterium
3GCF_A    282 ------ptlIQYEFYVPISETQHEYFQVLQRKvegpedvktfeveFEERWRDda--lHGFN-DDDVWAREAQQEFyger- 351 Nocardioides aroma...
1Z03_A    310 ------ehvVQYEWYVPITDDTHEYWEILVRVcptdedrkkfqyrYDHMYKPlc--lHGFN-DSDLYAREAMQNFyydg- 379 Pseudomonas putida
3GKE_A    239 tpkeqsihsRGTHILTPETEASCHYFFGSSRNfgid------dpeMDGVLRSw---qAQALvKEDKVVVEAIERRrayve 309 Stenotrophomonas m...
3GB4_A    239 tpkeqsihsRGTHILTPETEASCHYFFGSSRNfgid------dpeMDGVLRSw---qAQALvKEDKVVVEAIERRrayve 309 Stenotrophomonas m...
3GOB_A    239 tpkeqsihsRGTHILTPETEASCHYFFGSSRNfgid------dpeMDGVLRSw---qAQALvKEDKVVVEAIERRrayve 309 Stenotrophomonas m...
3GTE_A    239 tpkeqsihsRGTHILTPETEASCHYFFGSSRNfgid------dpeMDGVLRSw---qAQALvKEDKVVVEAIERRrayve 309 Stenotrophomonas m...
BAC65426  311 -------ntLAVRQIVPKGQEAFELVWTHFGYaddda----emqaIRRKQTNligpaGLIS-MEDGEAVEIVQNAivgeq 378 Sphingomonas sp. P2
Feature 1                                                  
1EG9_A    378 srdsDLLSNlgfgedvygdavypgvvgksaiGETSYRGFYRAYQA 422 Pseudomonas putida
2DE7_C    348 kgwvNEILFe---------------------SDEAIVAWRKLASE 371 Janthinobacterium
2DE7_A    348 kgwvNEILFe---------------------SDEAIVAWRKLASE 371 Janthinobacterium
3GCF_A    352 dgwsKEQLFp---------------------PDMCIVKWRTLASE 375 Nocardioides aromaticivorans
1Z03_A    380 tgwdDEQLVa---------------------TDISPITWRKLASR 403 Pseudomonas putida
3GKE_A    310 angiRPAMLs---------------------CDEAAVRVSREIEK 333 Stenotrophomonas maltophilia
3GB4_A    310 angiRPAMLs---------------------CDEAAVRVSREIEK 333 Stenotrophomonas maltophilia
3GOB_A    310 angiRPAMLs---------------------CDEAAVRVSREIEK 333 Stenotrophomonas maltophilia
3GTE_A    310 angiRPAMLs---------------------CDEAAVRVSREIEK 333 Stenotrophomonas maltophilia
BAC65426  379 qatsFIAMG----------------------GGRAEDADHLVTEG 401 Sphingomonas sp. P2

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