1IRX


Conserved Protein Domain Family
LysRS_core_class_I

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cd00674: LysRS_core_class_I 
Click on image for an interactive view with Cn3D
catalytic core domain of class I lysyl tRNA synthetase
Class I lysyl tRNA synthetase (LysRS) catalytic core domain. This class I enzyme is a monomer which aminoacylates the 2'-OH of the nucleotide at the 3' of the appropriate tRNA. The core domain is based on the Rossman fold and is responsible for the ATP-dependent formation of the enzyme bound aminoacyl-adenylate. It contains the characteristic class I HIGH and KMSKS motifs, which are involved in ATP binding. The class I LysRS is found only in archaea and some bacteria and has evolved separately from class II LysRS, as the two do not share structural or sequence similarity.
Statistics
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PSSM-Id: 173900
View PSSM: cd00674
Aligned: 12 rows
Threshold Bit Score: 491.839
Threshold Setting Gi: 27805745
Created: 24-Sep-2002
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 12 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment: ATP and tRNA binding sites added based on similarity to GluRS
  • Comment:tRNA is required for amino acid activation

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                  # #     #  #  #                           #     #  
1IRX_B      2 VHWADYIADKIIRErge----kekYVVESGITPSGYVHVGNFRELFTAYIVGHALRDKG---YEVRHIHMWDDYDRFRkv 74  Pyrococcus horikoshii
NP_832294   1 MHWAYEVAHELIRKhpn----kemFVCASGISPSGSVHIGNFREIVTTYFVVRALQDLG---KKTRFIFSWDDYDRFRkv 73  Bacillus cereus AT...
Q57959      1 MHWADVIAEKLIEErka-----dkYIVASGITPSGHIHVGNARETLTADAIYKGLINKG---VEAELIFIADTYDPLRkl 72  Methanocaldococcus...
O83650      8 LHWADKVAHKIIKErad----cdqYTCASGITPSGTVHIGNFREIISVDLVVRALRDQG---KSVRFVHSWDDYDVFRri 80  Treponema pallidum
Q8TTA3      1 MHWADVIAEDVLNKsg-------kHLVATGITPSGHIHIGNMREVVTADAAYRALLDMG---ADARLIYIADNYDPLRkv 70  Methanosarcina ace...
O29052      1 MHWADVIAADLLKRsn-------sHRIATGISPSGHIHLGNLREMVTADAIRRALLDAG---GEAKIVYIADDFDPLRrr 70  Archaeoglobus fulg...
NP_787190  17 QDWVSKLAEQVIQErkg----dkkIVVASGISPSGQIHLGNLREVITGHFIADEISSRG---FDCEHILSWDDFDRLRkv 89  Tropheryma whipple...
O27585     22 KHWIERIADELKERdv------eeHVVASGTSISGSIHIGNSCDVFIASSIAKSLKKDG---FKSRTVWIADDHDPLRkv 92  Methanothermobacte...
Q9ZDF8     12 NAWPFIEAKKILDSlngkapekgyILFETGYGPSGLPHIGTFAENARMVMVQKAFEQLSd--IPTKLICFSDDMDGLRkv 89  Rickettsia prowazekii
O51603      4 AHWADFYAEKIKKEkgp----knlYTVASGITPSGTVHIGNFREVISVDLVARALRDSG---SKVRFIYSWDNYDVFRkv 76  Lyme disease spiro...
Q98BC8     21 KAWPFEEAKKIIERykgge-fpetILFETGYGPSGLPHIGTFGEVARTSMVRHAFRVLTqdkVATKLLCFSDDMDGMRki 99  Mesorhizobium loti
Q9X895     11 TDWVSRFADEVIAEserrapgkpgVVVASGLSPSGPIHLGNLREVMTPHLVADEVRRRG---HEVRHLISWDDYDRYRkv 87  Streptomyces coeli...
Feature 1                                                                                     
1IRX_B     75 prnv---pqewkdYLGMPISEVPDPWGCHe-sYAEHFMRKFEEEVEKLGIEVDLLYASELYKRGEYSEEIRLAFEKRDKI 150 Pyrococcus horikoshii
NP_832294  74 pkni---dpsfakYIGMPYCDIPDPYGCHn-sYAEHFEKEFEKSLQAFGIEVEFIYQHDEYRSGRYNKNILEALYKRKEI 149 Bacillus cereus AT...
Q57959     73 ypfl---pkefeqYIGMPLSEIPCPEGCCe-sYAEHFLRPYLESLDDLGVELTTYRADENYKKGLYDEKIKIALDNREKI 148 Methanocaldococcus...
O83650     81 pdnvp-aqdelkqYIRMPITSVPDPFQQEd-sYARHHEREIESALPEVGIYPEYVYQSKQYQAGVYAQEIKIALDNRHRI 158 Treponema pallidum
Q8TTA3     71 ypfl---pesyaeHVGKPISEVPCPCGDCa-nYAEHFLKPFIEALRRLGINPEVLRADEMYRAGLYTEAIKTALAKRDEI 146 Methanosarcina ace...
O29052     71 ypfl---peeyenYVGMPLCKIPDPEGCHd-sYSEHFLQPFLESLEILGIPVEVRRAYQMYSEGLYENNTRIALKRRDEI 146 Archaeoglobus fulg...
NP_787190  90 pani---dkgyeqYIGMPLCDVPPPAGSKknsWSEHFRGDFEKTMEILGIKVRHISQSQMYRSGAYTHNILLALEKRHEI 166 Tropheryma whipple...
O27585     93 pypl---pesyekYLGVPYSMIPCPEGCCe-sFVEHFQRPFLEALERFRIGVEHYSGARMYTEGLYNDYIRTSLERAPEI 168 Methanothermobacte...
Q9ZDF8     90 psnip-hpemvaqYMDMPLTSIPDPFGKCk-sYGHYMNAKLCAFLDKFGFKYEFYSSTNCYKAGMFDEMLIRVLEKYDEI 167 Rickettsia prowazekii
O51603     77 pknmp-eqellttYLRQAITRVPDTRSHKt-sYARANEIEFEKYLPVVGINPEFIDQSKQYTSNAYASQIKFALDHKKEL 154 Lyme disease spiro...
Q98BC8    100 pdnvp-draalepHLHKPLSSVPNPFGGDyasFADHNNAMLCRFLDTFGFDYEFASATQYYKSGRFDAMLLRAAERYDKI 178 Mesorhizobium loti
Q9X895     88 pagvpgvdeswaeHIGKPLTSVPAPKGSPhpnWAEHFKAAMVDSLAEMGVEFDGISQTAQYTSGVYREQILHAMKHRRDI 167 Streptomyces coeli...
Feature 1                                                                                     
1IRX_B    151 MEILNKYREIAkqpp---------------------------lpenWWPAMVYCPEHr--REAEIIEWDg-gWKVKYKCP 200 Pyrococcus horikoshii
NP_832294 150 YDILMGFKTREcsee---------------------------eresFYPATLYCERCg-kDTTTITHFDevlKTVRYECE 201 Bacillus cereus AT...
Q57959    149 MEILNKFRANPlp-------------------------------ddWWPINIVCENCg-kLKTKVIKYDsekEEITYRCE 196 Methanocaldococcus...
O83650    159 QAILNEYRDEQhki-----------------------------sgtYWPVSVFCTACh-kDCTTVDAWDs-hWCLQYHCE 207 Treponema pallidum
Q8TTA3    147 AKILEEVSGKTva-------------------------------edWSPFNPRCNECgkiTTTKVAGFDleaETVDYVCA 195 Methanosarcina ace...
O29052    147 ARIIAEVTGREle-------------------------------erWYPFMPLCENCgriNSTRVTSFDe--NWIYYECD 193 Archaeoglobus fulg...
NP_787190 167 QNIIGKYQSKKtqpae--------------------------spreYYPYVVYCTRCk-kDLTQIEDFAg--SELSYSCK 217 Tropheryma whipple...
O27585    169 REIFNRFRDRPlr-------------------------------ddWLPYNPICEKCg--RVNTTEAYDfsgDTVRYRCE 215 Methanothermobacte...
Q9ZDF8    168 MALMLPTFRDErk-------------------------------ttYAPFMPICPKTgkvLQVPIEKWDakaGTVSYKDE 216 Rickettsia prowazekii
O51603    155 SEALNEYRTSKle-------------------------------enWYPISVFCTKCn-rDTTTVNNYDn-hYSVEYSCE 201 Lyme disease spiro...
Q98BC8    179 MAVMLPTLGPErq-------------------------------atYSPFLPISPKSgrvLYVPMKHVDakaGTITFDDE 227 Mesorhizobium loti
Q9X895    168 DAILDQYRTKKapakksqkpldeaeleaaegsgaaaeddgssgsagYFPYKPYCGNCe-kDLTTVTAYDddsTELTYACT 246 Streptomyces coeli...
Feature 1                        #   #                 #                            #         
1IRX_B    201 e-GHEGWVDIr-SGNVKLRWRVDWPMRWSHFGVDFEPAGKDHLVag-ssYDTGKEIIKeVYGKEAPLSLMYEFVGIKGqk 277 Pyrococcus horikoshii
NP_832294 202 c-GNEKELSVlnTNKMKLNWKIDWPMRWMIEDVIFEPGGRDHSSet-gsYNVSKEIARkIFNREVPHYVAYDFIGIKGnh 279 Bacillus cereus AT...
Q57959    197 icGFENTVKPy-KGRAKLPWRVDWPARWSIFNVTIEPMGKDHAAag-gsYDTGVLIAKeIYNYIPPKKVVYEWIQLKVgd 274 Methanocaldococcus...
O83650    208 c-GHGEQVDLrqTSAVKLSWRVDWAMRWSKEHVVFEPAGKDHHSqg-gsFDTARLISDhIYHWPAPVSFRYDFIGLKGlp 285 Treponema pallidum
Q8TTA3    196 c-GHSGTVPM--AGGGKLTWRVDWPARWAVLGVTVEPFGKDHASkg-gsYDTGKRIVReIYGHEPPFPIVYEWIMLGKr- 270 Methanosarcina ace...
O29052    194 c-GHSGRVGY--VGGGKLTWRVDWAARWQILSITCEPFGKDHAAag-gsYDTGVRIAReIFDYEPPYPVPYEWIHLKGk- 268 Archaeoglobus fulg...
NP_787190 218 c-GHSETIGIgeFNNGKLPWKVDWPMRWMYESVDFEPGGVDHCSpgsasLSASSDIANqIFNWTPPLTVMYSFVGVQGh- 295 Tropheryma whipple...
O27585    216 c-GFDGEMDIk-SGLGKLTWRVEWAARWKILGVTCEPFGKDHAAsg-gsYDVSSIISEeIFDYPAPYPVPYEWITLRGe- 291 Methanothermobacte...
Q9ZDF8    217 d-GNDVEVPVt-GGHCKLQWKPDFGMRWAALKVDYEMYGKDHLAn----SRLYSEICR-ILGGKPPVQFCYELFLDANg- 288 Rickettsia prowazekii
O51603    202 c-GNQESLDIrtTWAIKLPWRIDWPMRWKYEKVDFEPAGKDHHSsg-gsFDTSKNIVK-IFQGSPPVTFQYDFISIKGrg 278 Lyme disease spiro...
Q98BC8    228 --GTETTLSIt-GGRVKLQWKPDFGMRWAALGVDFEMFGKDHQTn----AVVYDRICN-ILGGRAPEHFVYELFLDENg- 298 Mesorhizobium loti
Q9X895    247 acGFSETVRLseFNRGKLVWKVDWPMRWAYEGVVFEPSGVDHSSpg-ssFQVGGQIVG-IFGGEQPIGPMYAFVGISGm- 323 Streptomyces coeli...
Feature 1          #                                                                          
1IRX_B    278 --gKMSGSKGNVILLSDLYEVLEPGLVRFIYARhr--pNKEIKIDlglgILNLYDEFEKVERIYFgvegg-------kgd 346 Pyrococcus horikoshii
NP_832294 280 --eKMSSSSGNSITPSNLLKVYIPEVILFMFAKykpgaAFHIGLDe--dVIRNYTEYERLKDSYEnktlknedlfdaiki 355 Bacillus cereus AT...
Q57959    275 kaiPMSSSKGVVFAVKDWTNIAHPEILRFLLLRsk--pTKHIDFDlk-kIPDLVDEYDRLEDFYFnnkdkd----elsee 347 Methanocaldococcus...
O83650    286 --gKMSSSAGKVVGLRDVLEVYQPEVLRYLFVStrpntEFSISFDl--dVLKIYEDYDKSERVAWgihaak-----sehe 356 Treponema pallidum
Q8TTA3    271 --gAMSSSTGVVVSISDMLEVVPPEVLRYLIIRtk--pEKHIQFDpgqpLLNLVDEYERLRDKFRendp---------sl 337 Methanosarcina ace...
O29052    269 --gAMKSSKGIVLPVREMVEVIPPEIVRYITIRvk--pERHIEFDpglgLLDLVEEFEEKFKEKDr-------------- 330 Archaeoglobus fulg...
NP_787190 296 --aKMSSSTGNVLTPNDALSVMEPTLLRWMYARrkpnqSFTVGFDq--sLQRLYDEWDSLPNDLTgcelassy-tramst 370 Tropheryma whipple...
O27585    292 ---AMSKSRGVFFTPGQWLEIGPPESLNYFIFRskpmkHKDFNPDm--pFLDLMDQFDRTERIYYgmedaa-----seke 361 Methanothermobacte...
Q9ZDF8    289 --eKISKSRGNSISVDDWLKYASVESIALFMYKnp-arAKRLFFDl---IPKNVDEYITLNQKYHleed---------mv 353 Rickettsia prowazekii
O51603    279 --gKISSSSGDVISLKDVLEVYTPEVTRFLFAAtkpntEFSISFDl--dVIKIYEDYDKFERIYYgvedv------keek 348 Lyme disease spiro...
Q98BC8    299 --qKISKSKGNGLTIDEWLTYAPTESLGLYMYQrp-rqAKKLYFDv---IPRAVDEYYTFLGAYPrqd----------wk 362 Mesorhizobium loti
Q9X895    324 --aKMSSSKGGVPTPADALKIMEPQLLRWLYARrrpnqSFKIAFDq--eIQRLYDEWDRLDAKVAdgsalpa--daaaha 397 Streptomyces coeli...
Feature 1                
1IRX_B    347 dEELRRTYELS 357 Pyrococcus horikoshii
NP_832294 356 sRVDSEIKEYP 366 Bacillus cereus ATCC 14579
Q57959    348 eQEKIRIYELS 358 Methanocaldococcus jannaschii
O83650    357 fMRHKRIYELS 367 Treponema pallidum
Q8TTA3    338 gDFEKRIYELS 348 Methanosarcina acetivorans
O29052    331 sVELSLVGEVV 341 Archaeoglobus fulgidus
NP_787190 371 tNKRFNAPEIL 381 Tropheryma whipplei str. Twist
O27585    362 eQKLRNIYRVS 372 Methanothermobacter thermautotrophicus str. Delta H
Q9ZDF8    354 tRFANPVYHIH 364 Rickettsia prowazekii
O51603    349 kRAFKRIYELS 359 Lyme disease spirochete
Q98BC8    363 eRLGNPVWHMH 373 Mesorhizobium loti
Q9X895    398 rAVGTAAGELP 408 Streptomyces coelicolor

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