1LI7,3C8Z


Conserved Protein Domain Family
CysRS_core

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cd00672: CysRS_core 
Click on image for an interactive view with Cn3D
catalytic core domain of cysteinyl tRNA synthetase
Cysteinyl tRNA synthetase (CysRS) catalytic core domain. This class I enzyme is a monomer which aminoacylates the 2'-OH of the nucleotide at the 3' of the appropriate tRNA. The core domain is based on the Rossman fold and is responsible for the ATP-dependent formation of the enzyme bound aminoacyl-adenylate. It contains the characteristic class I HIGH and KMSKS motifs, which are involved in ATP binding.
Statistics
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PSSM-Id: 173899
View PSSM: cd00672
Aligned: 42 rows
Threshold Bit Score: 242.87
Threshold Setting Gi: 28493283
Created: 24-Sep-2002
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 21 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1L17_A; E. coli CysRS bound to Cys and Zn2+ (based on associated journal article).
    View structure with Cn3D
  • Structure:3C8Z; Mycobacterium smegmatis Mshc with bound zinc and cysteinyl adenylate analogue, 5'-O-[N-(l-cysteinyl)-sulfamonyl]adenosine (CSA), defined using 4 A contacts.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                              ####     # ##  ##                     # #              
1LI7_A      3 KIFNTltRQKEEfkpihaGEVGMYVCGITVYDLCHIGHGRTFVAFDVVARYLRFLgYKLKYVRNITDIDDKIIKRANENG 82  Escherichia coli
3C8Z_A     22 RLFDSadRQVRPvt--pgPTATMYVCGITPYDATHLGHAATYLTFDLVHRLWLDAgHTVQYVQNVTDVDDPLFERAERDG 99  Mycobacterium smeg...
P41259      2 FIYDTklKQKVPfeplvqNKANIYVCGPTVYDDAHLGHARSAIAFDLLRRTLELSgYEVMLVRNFTDIDDKIINKALKEN 81  Helicobacter pylori
Q9HJ27      2 RFYNTltRDVDEfkemnsGRINMFVCGPTVQDHFHIGHARTYIFFDAVAKLLRNLgYSVFYLQNITDIDDKIINKAREMN 81  Thermoplasma acido...
Q9PPB8      2 RLLDSvtKEKIKld---kKDISIYLCGPTVYDDAHLGHARSSVCFDLLRRVLLAQgNRVKFARNYTDIDDKILKKMAQSG 78  Campylobacter jejuni
NP_787475   4 NLYDTlsRRIVTld--crDRVELYVCGPTVQSPPHIGHMRSGVVYDCLRRWLEYKgLPVLYVRNITDIDDKILASARSTE 81  Tropheryma whipple...
Q9PR21      2 KLYDSysNQLVEin---dELISIYNCGPTVYNHIHIGNARPLITMDVLYRFLKKHnIKTKYVLNITDIDDKIINYALANN 78  Ureaplasma parvum
Q8F525      5 QFYNSlsGRKEKfspsdpNRVTVYSCGPTVYNFAHIGNLRAFLFVDVLRRSLKLLgYGVDMTMNITDIDDKIIRDSIASK 84  Leptospira interro...
P47495      1 MIVDSvsQKPTTlv---qKTINIYLCGPTVYNDLHLGNTRPLIVFDVLNRVLKKAkYTVNFVQNITDIDDKIIKIAQQQE 77  Mycoplasma genitalium
NP_695505  32 NLYDTasHQVSHfvplkpGEVGIYVCGATVQSSPHIGHIRAAVAFDIVRRWFLKLgYKVTFVRNVTDIDDKILVKAAAAG 111 Bifidobacterium lo...
Feature 1                                                                                     
1LI7_A     83 ES--FVAMVDRMIAEMHKDFDALNILr-pDMEPRAthhiaeiielteqliakghayvadngdvmfdvptdptygvlsrqd 159 Escherichia coli
3C8Z_A    100 ID--WRTLGDRETQLFREDMAALRVLpphDYVAATdaiaevvemvekllasgaayivedaeypdvyfradataqfgyesg 177 Mycobacterium smeg...
P41259     82 KS--IQELSSIYIESYTRDLNALNVKk-pSLEPKAseyldamvgmietllekniayqvsngdiyldtskdkdygslsvhn 158 Helicobacter pylori
Q9HJ27     82 IQ--PQDVADMYLREFLEDMSALKVTs-vNYFAKStlyineiisqisrliekgyayetsdgvyfevskfadygqlsnqsl 158 Thermoplasma acido...
Q9PPB8     79 QT--LEEITEFYIKSYEEDMRVLNVLd-pDFKPRAthyitamldlikklakdgfvytledgiyfdtskdekylslsn--- 152 Campylobacter jejuni
NP_787475  82 TGetWWQIAYRVSGLFNEAYKALFVKp-pDYEPLVtahipdiikaieiliqknvayramdgsgnvffsidkhpsygeltd 160 Tropheryma whipple...
Q9PR21     79 LK--ELEVSEYYFNEYLKIKKALNTLe--MINPKVsthmdkiidyiqklidkqagyfigddvyfdtkkalnygqlskrd- 153 Ureaplasma parvum
Q8F525     85 KS--IIEFTAPWTKAFFEDLKTVSAEi-lEHYPKAtdsipemvdiiqklqkkglvykkdeslyfsiqkfqnygklskidt 161 Leptospira interro...
P47495     78 VS--ESVVTKQQITAYKSLLKKLNILp--IKHIQItekidkipdyidqlvnqnhayvstqnnvyfavnslkqygyla--- 150 Mycoplasma genitalium
NP_695505 112 QR--WWARAYYYEREFTEAYNTLGVLp-pTVEPRAtghmsdmidliqrildnghgyvvtdadgkptgnvyfdvaswphyg 188 Bifidobacterium lo...
Feature 1                                                                                  #  
1LI7_A    160 --------------------------------ldqlqagarvdvvddkrnpmdfvlwkmskegepswpspwgagrpgWHI 207 Escherichia coli
3C8Z_A    178 ydr--------------------------dtmltlfaerggdpdrpgksdqldallwraerpgepswpspfgrgrpgWHV 231 Mycobacterium smeg...
P41259    159 ---------------------------------ssiefgriglvqekrleqdfvlwksykgdndvgfdsplgkgrpgWHI 205 Helicobacter pylori
Q9HJ27    159 ---------------------------------dqiihgyrvavnenkrnpedfvlwkkrkpgepywdspwgpgrpgWHI 205 Thermoplasma acido...
Q9PPB8    153 -----------------------------------rnleenisrlsnevqkrnesdfvlwkfdenfyesefgkgrpgWHT 197 Campylobacter jejuni
NP_787475 161 q-------------------------------kdlliddcitpgkrdprdftlwkekkdtdpdlafwespwgpgrpgWHI 209 Tropheryma whipple...
Q9PR21    154 ---------------------------------lendivgmriesaankhnpndfilwkktnkgimwntpwgigrpgWHS 200 Ureaplasma parvum
Q8F525    162 s-------------------------------gmktgtrydtdeyekedvrdfvlwkspklegetswdtlvgtgrpgWHL 210 Leptospira interro...
P47495    151 ------------------------------------nrmvhleetdtdkknkldfvlwkittagikwnskwglgrpgWHV 194 Mycoplasma genitalium
NP_695505 189 elthqkqtsevdeaaavadrmgpsvdatgadkynpvdpadaspdkhdprdfalwkapkdtdpedarwstpfgvgrpgWHI 268 Bifidobacterium lo...
Feature 1      #                     ## ##   #                        ###                     
1LI7_A    208 ECSAMNCKQLG-----NHFDIHGGGSDLMFPHHENEIAQSTCAHDg-qYVNYWMHSGMVMVDREKMSKSLGNFFtvrd-v 280 Escherichia coli
3C8Z_A    232 ECSAIALTRIG-----TGLDIQGGGSDLIFPHHEYSAAHAESVTGerrFARHYVHTGMIGWDGHKMSKSRGNLVlvsqlr 306 Mycobacterium smeg...
P41259    206 ECSSMVFETLAltntpYQIDIHAGGADLLFPHHENEACQTRCAFGv-eLAKYWMHNGFVNINNEKMSKSLGNSFfvkd-a 283 Helicobacter pylori
Q9HJ27    206 EDTAITETYFG-----PEYDIHGGGSDLIFPHHEAEIAQMRAISGrkyLSHYWIHTGMINVNNEKMSKSLKNFVtire-v 279 Thermoplasma acido...
Q9PPB8    198 ECVAMIDSIFE-----NTLDIHAGGIDLLFPHHENEAAQCRCGCKr-kLANIWLHNGFVKIDGEKMSKSLNNSFfikd-a 270 Campylobacter jejuni
NP_787475 210 ECSVMATKYLG-----TRFAIHGGGVDLRFPHHENELAQARALGAh--FADIWMHTGAVNVEGIKMSKSFGNSVlvqd-a 281 Tropheryma whipple...
Q9PR21    201 ECSCLINTYIG-----EQVSIHGGGIDLKFPHHENENAQNQVLYNk-nLAKVWMHFGLVNINNEKMSKSLNNFIlvkd-l 273 Ureaplasma parvum
Q8F525    211 ECSAMIRKVYG-----SGVDIHTGGVDLLFPHHENEIAQSEGAFPeesFVKTWLHSEHLLVDGQKMSKSKGNFYtlrdli 285 Leptospira interro...
P47495    195 ECAFLIDYCFK-----NELTIHGGGVDLKFPHHENENALHMALYNq-pITKHWMHIGHLMIENQKMSKSLQNFLlavd-f 267 Mycoplasma genitalium
NP_695505 269 ECSAMSHRYLG-----DGFDIHGGGLDLRFPHHENEMAQTCAAGYp--SAARWMHSAWVTAKGEKMSKSLGTGLsvps-v 340 Bifidobacterium lo...
Feature 1                              
1LI7_A    281 lkyYDAETVRYFLMSGHYRSQLNYS 305 Escherichia coli
3C8Z_A    307 aqgVDPSAIRLGLFSGHYREDRFWS 331 Mycobacterium smegmatis
P41259    284 lknYDGEILRNYLLGVHYRSVLNFN 308 Helicobacter pylori
Q9HJ27    280 lkeYRPEDLRYALLNANYRTQLDFS 304 Thermoplasma acidophilum
Q9PPB8    271 lkeFMGEALRFYLLSSHYRSHFNYS 295 Campylobacter jejuni
NP_787475 282 lskVSPSALRYYFLTAHYRSTLNYT 306 Tropheryma whipplei str. Twist
Q9PR21    274 laeYDYQVVRWFFYQADYKQPIKFS 298 Ureaplasma parvum
Q8F525    286 qqgLDPKAIRFLLISTHYRSKLNFS 310 Leptospira interrogans
P47495    268 lnfHDFRVLRWIFYQKHYLHPIDLN 292 Mycoplasma genitalium
NP_695505 341 laeHSAWVVRYALGSVQYRSMLEWS 365 Bifidobacterium longum NCC2705

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