12AS


Conserved Protein Domain Family
AsnA
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cd00645: AsnA 
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Asparagine synthetase (aspartate-ammonia ligase) (AsnA) catalyses the conversion of L-aspartate to L-asparagine in the presence of ATP and ammonia. AsnA is a homodimeric enzyme which is structurally similiar to the catalytic core domain of class II aminoacyl-tRNA synthetases. Ammonia-dependent AsnA is not homologous to the glutamine-dependent asparagine synthetase AsnB.
Links
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Statistics
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PSSM-Id: 238350
Aligned: 11 rows
Threshold Bit Score: 541.162
Created: 24-Sep-2002
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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active sitedimer interface
Conserved site includes 11 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:12AS_A; E.coli Asn_A bound to ADP and Asn
  • Citation:PMID 9437423
Scroll to Sequence Alignment Display
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                                        #  #           
12AS_A        9 QRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGTQDNLSGAEKAVQVKVKalpDAQFEVVHSLAKWKRQTLGQHDFSA 88  Escherichia coli
P54264       19 RKRQSATSGKPSSEFGTAMNLERISAPMFVKKSSGLNDNLSGWEKPVSFTLHdgnEGELQIVHSLAKWKRWALKHYGFSH 98  Lactobacillus de...
Q9PQC8       13 QKAIVEIKNSFQKHFAKNLNLSRVTAPLFVEGQSGLNDHLDHKQKAVSFYAKk-lDKTLEIVQSLAKWKRLALLDYGFSL 91  Ureaplasma parvum
Q97NQ0        9 QEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGMQDNLSGVENPVSVKVLqipDATYEVVHSLAKWKRHTLARFGFGE 88  Streptococcus pn...
O83567        9 QQGISFAKHTFTQKLMEHLGLIEVQGPLLSQVGDGIQDGLSGREKAVSVSVKqipGTAFEVVHSLAKWKRHTLARYGFQD 88  Treponema pallidum
NP_757535    10 QKAIKFIKDLFQVNLAHALKLHRVTAPLVLERNKGINDDLNGSENPVTFTSDg-nGISGEIPQSLAKWKRMMLGKYEIPL 88  Mycoplasma penet...
ZP_00046862  36 EAAIVFIRETFQDKIAEKLNVQRMSAPMFVEKSTGLNDNLNGVERPVSFDMKampNDTIEVVHSLAKWKRLALKRYGFGM 115 Lactobacillus ga...
NP_853398    12 ELAIKYIKDLFQNALSKELNLLRVSAPLIIAPDSGLNDNLNGWEAPVSFKSKt-nGVSSQVVQSLAKWKRYSIARYEIPL 90  Mycoplasma galli...
NP_844231    12 QIAIKEVKTFFEDQLAKRLELFRVSAPLFVTKKSGLNDHLNGVERPIEFDMLh-sGEELEIVHSLAKWKRFALHEYGYEA 90  Bacillus anthrac...
Q8XIY5       20 EVAIKELKDFFEDSLAKNLNLTRVSAPLFVNKGSGLNDDLNGIERPVSFDMKampEFNIQIVHSLAKWKRLALHRYEFEH 99  Clostridium perf...
NP_811042    20 ELGIKQIKEFFQLNLSSELRLRRVTAPLFVLKGMGINDDLNGIERPVSFPIKdlgDAQAEVVHSLAKWKRLTLADYNIEP 99  Bacteroides thet...

Feature 1                  #               # # #                                                
12AS_A       89 GEGLYTHMKALRPDEDrlSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEF---GLAPFLPDQI 165 Escherichia coli
P54264       99 GEGLFTNMNAIRKDEEvlDNLHSVYVDQWDWEKVIDKSERTEATLRQTVQRIFETIKGMEYHVRALYp--QAAYHLPEEI 176 Lactobacillus de...
Q9PQC8       92 YEGLYTDMNAIRADDDi-DEIHSIYVDQWDWEILINNQDCNLDFLKSIVNKIYSTIRIVQLEIDQLYn--PKQIILPDSI 168 Ureaplasma parvum
Q97NQ0       89 GEGLFVHMKALRPDEDslDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARY---DIESILPKQI 165 Streptococcus pn...
O83567       89 NEGLFVHMIALRPDEDflDQVRSVCVDQWDWEKVVPVGSRNLAYLKDTVRKVYGALRESEVLVSERF---GLRAFLPADI 165 Treponema pallidum
NP_757535    89 HEGIYADMNAIRKDESl-SSIHSIYVDQWDWELHIKKTERNLETLKVVVKKIYEIIRLCQKEVNKKYe-wFAENLLPEEI 166 Mycoplasma penet...
ZP_00046862 116 HEGLYTNMNAIRRDEDl-DNFHSIYVDQWDWEKIISKDERNIDTLKDTVKQIFKAIKETEKEVAARYp--SSTYRLPNEI 192 Lactobacillus ga...
NP_853398    91 YQGLYTDMNAIRMDETl-DATHSMYVDQWDWELRISENDRNVEFLKNTVNKIYKVLKEAQLKVNEKYgifEQKDLLPEHI 169 Mycoplasma galli...
NP_844231    91 GEGLYTNMNAIRRDEEl-DATHSIYVDQWDWEKIVQKEWRTVDYLQKTVLTIYGIFKDLEDHLFEKYp--FLGKYLPEEI 167 Bacillus anthrac...
Q8XIY5      100 GEGLYTDMNAIRRDEDl-DNIHSIYVDQWDWEKIIDKEERNLETLKETVKSIYGTFKATEDFIVAKYp--HIEKILPEDI 176 Clostridium perf...
NP_811042   100 GYGIYTDMNAIRSDEEl-GNLHSLYVDQWDWERVITNEDRNVEFLKEIVNRIYAAMIRTEYMVYEMYp--QIKPCLPQKL 176 Bacteroides thet...

Feature 1                                                                             #         
12AS_A      166 HFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHR-HDVRAPDYDDWstpselghagLNGDILVWNPVLE 244 Escherichia coli
P54264      177 SFVTSEELEARWPSLTPSEREDKICQEKGAVFLEHIGGALPLSKKpHDLRAPDYDDWt---------LNGDLLFWYEPLQ 247 Lactobacillus de...
Q9PQC8      169 TFISSQELEDLYPHLSPSRREYEFAKIHKAIFIYQIGYPLKSGYI-QSIRSPEYDNWn---------LNGDLIVYHKLND 238 Ureaplasma parvum
Q97NQ0      166 TFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKP-HDGRAPDYDDWtsesengykgLNGDILVWNESLG 244 Streptococcus pn...
O83567      166 VFVQSEELVRRYPHLDSKGREDAICKEHGAVFLIGIGGVLSDGKP-HDVRAPDYDDWttpsegeykgLNGDILVWNPVLG 244 Treponema pallidum
NP_757535   167 TFISSEDLLQRYPNKTPKERERLIASKYKAVFIIGIGDNLSDGKP-HDLRAPDYDDWk---------LNGDIIVWNETTK 236 Mycoplasma penet...
ZP_00046862 193 TFITTQELEDRWPDLTPDEREDKIAKEKKAVFLMKIGDKLKRSGKpHDGRAPDYDDWq---------LNGDLLFWYEPLQ 263 Lactobacillus ga...
NP_853398   170 HFVTTQELLDQYPDKNPSEREQLVCEKYKAVFVMQVGKKLSNNQV-HDGRSPDYDDWs---------LNGDIMVYNPRSK 239 Mycoplasma galli...
NP_844231   168 VFVTSQELEDKYPELTPKDREHAIAKEHGAVFIIGIGDALRSGEK-HDGRAADYDDWk---------LNGDILFWHPVLQ 237 Bacillus anthrac...
Q8XIY5      177 TFITSQELEDRYPDLTSKERETAICKEFGAVFIIGIGGKLASGEK-HDDRSPDYDDWt---------LNGDLLFYYPLFD 246 Clostridium perf...
NP_811042   177 HFIHSEELRQLYPNLEPKCREHAICQKYGAVFIIGIGCKLSDGKK-HDGRAPDYDDYtstglnnlpgLNGDLLLWDDVLQ 255 Bacteroides thet...

Feature 1          #  #   #                                            #                        
12AS_A      245 DAFELSSMGIRVDADTLKHQLALTGded-rleLEWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHiGQVQAGVWPAAVR 323 Escherichia coli
P54264      248 RAFEVSSMSIRVDEDRLQEQLKLAGaed-rldLPFHQALLKGDLPYSIGGGIGQSRLCMLLLQGPH-GEVQASIWPDEIV 325 Lactobacillus de...
Q9PQC8      239 QAIELSSMGIRVSKQDFIKQTNFANlkndqenNFYHQMILNNQLPQTIGGGIGQSRLCMFLLNKKHiGEVQVSVWPNEYK 318 Ureaplasma parvum
Q97NQ0      245 GAFELSSMGIRVDEETLRRQVEITGded-rleLEWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHiGEVQTSVWPQEVR 323 Streptococcus pn...
O83567      245 RAFEVSSMGIRVDEGALRTQLALTGded-slaCSWHQDLINGRLPQSIGGGIGQSRLAMLLLQRKHiGEVQASVWPRSVR 323 Treponema pallidum
NP_757535   237 SALELSSMGIRVDEVSLVEQLDKSNnns-rkeLDFHKKLINKEFPYSIGGGIGQSRLCYFLLHKQHiGEVQSSLWPKDIL 315 Mycoplasma penet...
ZP_00046862 264 RKLEISSMGIRVSEESLKTQLKKAHaee-raaLPFHKMLLNGELPYTIGGGIGQSRLCMLLLGKAHiGEVQASIWPPKMI 342 Lactobacillus ga...
NP_853398   240 KALELSSMGIRVNKEVLLKQLEESKqne-rleLMFHKKLVNGELHQTIGGGIGQSRLCYFLLQKDHiGEVQASHWSDEIV 318 Mycoplasma galli...
NP_844231   238 SSFELSSMGIRVDSKSLDEQLTKTGedf-kreYDFHKGILEDVLPLTIGGGIGQSRMCMYFLRKAHiGEVQSSVWPDDLR 316 Bacillus anthrac...
Q8XIY5      247 EAVELSSMGIRVDEESLLKQLKIAEcee-rkeLPFHQMLLEGKLPYTIGGGIGQSRICMFFLRKAHiGEVQASMWDEDMI 325 Clostridium perf...
NP_811042   256 RSIELSSMGVRVDREALQRQLKEENeee-rlkLYFHKRLMDDTLPLSIGGGIGQSRLCMFYLRKAHiGEIQASIWPEDMR 334 Bacteroides thet...

Feature 1          
12AS_A      324 ESV 326 Escherichia coli
P54264      326 EKC 328 Lactobacillus delbrueckii subsp. bulgaricus
Q9PQC8      319 DEL 321 Ureaplasma parvum
Q97NQ0      324 DTY 326 Streptococcus pneumoniae
O83567      324 EEF 326 Treponema pallidum
NP_757535   316 EEA 318 Mycoplasma penetrans
ZP_00046862 343 EEC 345 Lactobacillus gasseri
NP_853398   319 VEA 321 Mycoplasma gallisepticum R
NP_844231   317 EAC 319 Bacillus anthracis str. Ames
Q8XIY5      326 RTC 328 Clostridium perfringens
NP_811042   335 KEC 337 Bacteroides thetaiotaomicron VPI-5482

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