1BK9,1POC,1A2A,1POE,1LE6,1BUN,1PSH,2NOT,1OZY


Conserved Protein Domain Family
PLA2_like

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cd00618: PLA2_like 
Click on image for an interactive view with Cn3D
PLA2_like: Phospholipase A2, a super-family of secretory and cytosolic enzymes; the latter are either Ca dependent or Ca independent. PLA2 cleaves the sn-2 position of the glycerol backbone of phospholipids (PC or phosphatidylethanolamine), usually in a metal-dependent reaction, to generate lysophospholipid (LysoPL) and a free fatty acid (FA). The resulting products are either dietary or used in synthetic pathways for leukotrienes and prostaglandins. Often, arachidonic acid is released as a free fatty acid and acts as second messenger in signaling networks. Secreted PLA2s have also been found to specifically bind to a variety of soluble and membrane proteins in mammals, including receptors. As a toxin, PLA2 is a potent presynaptic neurotoxin which blocks nerve terminals by binding to the nerve membrane and hydrolyzing stable membrane lipids. The products of the hydrolysis (LysoPL and FA) cannot form bilayers leading to a change in membrane conformation and ultimately to a block in the release of neurotransmitters. PLA2 may form dimers or oligomers.
Statistics
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PSSM-Id: 153092
View PSSM: cd00618
Aligned: 101 rows
Threshold Bit Score: 43.3225
Threshold Setting Gi: 7657126
Created: 1-Nov-2000
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
catalyticprimary metal
Conserved site includes 2 residues -Click on image for an interactive view with Cn3D
Feature 1:catalytic residues [active site]
Evidence:
  • Structure:1POC: Bee venom phospholipase A2 bound with a transition state analogue, contacts at 3.5 A.
    View structure with Cn3D
  • Citation:PMID 2274788
  • Structure:1POE; human secretory phospholipase A2 bound with transition-state analog (1-o-octyl-2-heptylphosphonyl-sn-glycero-3-phosphoethanolamine), contacts at 3.5 A.
    View structure with Cn3D
  • Citation:PMID 1948070
  • Citation:PMID 9276469
  • Citation:PMID 9013608

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                             #                          
1BK9          22 SNYGCYCGWGgq--------------------------gRPq--DATDRCCFVHDc-CYgkvtgcdpkmdvysfseengd 72  halys viper
1POC           3 YPGTLWCGHGnkss----------------------gpnELgrfKHTDACCRTHDm-CPdvmsageskhg---------- 49  honeybee
ABI52736      30 FPDTKWCGAGnvsn----------------------gtgDYgaaRRTDMCCEIHDn-ATdyilagksyanh--------- 77  Argas monolakensis
XP_868232      9 LPSWERGHLLagvasstd--------------astfsseGEf--KDPDRCCWKHKq-CTghiihpftsdy---------- 61  dog
EAW51126       9 LPSWERGHLLagvasst---------------dvstfseGDc--KEPDKCCWRHKq-CTghiiypfasdc---------- 60  human
NP_001020929  32 LPSWKRGYPAsvesssd----------------mssfseGEn--KETERCCWKHQq-CPvhiihsfsdcg---------- 82  Norway rat
XP_587287      7 LRIERWTKEKteddtss---------------wdesrvrGEf--KNTDRCCWKHK--CAghivrpfspdc---------- 57  cattle
XP_001108990   7 LTIERWTKEKtepkvrs---------------wdesrccGDc--KEPDKCCWKHKq-CTghiiypfasdc---------- 58  rhesus monkey
BAC32640       3 WTAEGRLLYKvgiyellhsiqa------paplcslslhaGVf--HGPDLCCREHDq-CPqtisplqynyg---------- 63  house mouse
NP_056530    316 KGSKRPSKANttalqdpmvsprldvaptglqgpqgglkpQGa--RWVCRSFRRHLdqCEhqigpreiefq---------- 383 human
Feature 1                        #          
1BK9          73 ivcggddpckkeICECDRAAAICFRDN 99  halys viper
1POC          50 --ltntashtrlSCDCDDKFYDCLKNS 74  honeybee
ABI52736      78 sslrnprphtvtHCKDDMKLFDCLYND 104 Argas monolakensis
XP_868232     62 -ghhnlhlhsvsHCDCDSRLKDCSEKT 87  dog
EAW51126      61 -vrhslhlhsvnHCNCNSRLKDSSEDS 86  human
NP_001020929  83 --hhnrcmhavsHCNCESRCQSYRPIS 107 Norway rat
XP_587287     58 -vhhdvhlhslsHCDCDSRLKDCSEKT 83  cattle
XP_001108990  59 -vchslhlrsvnHCNCNSRLKDCSEDS 84  rhesus monkey
BAC32640      64 --irnfrfhtisHCDCDARCRAYGSTP 88  house mouse
NP_056530    384 --llnsaqeplfHCNCTRRLARFLRLH 408 human

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