1N8P,1E5E,1CL2,1IBJ,1CS1,1I41,1IBJ,1Y4I,2CTZ,1PG8


Conserved Protein Domain Family
CGS_like

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cd00614: CGS_like 
Click on image for an interactive view with Cn3D
CGS_like: Cystathionine gamma-synthase is a PLP dependent enzyme and catalyzes the committed step of methionine biosynthesis. This pathway is unique to microorganisms and plants, rendering the enzyme an attractive target for the development of antimicrobials and herbicides. This subgroup also includes cystathionine gamma-lyases (CGL), O-acetylhomoserine sulfhydrylases and O-acetylhomoserine thiol lyases. CGL's are very similar to CGS's. Members of this group are widely distributed among all three forms of life.
Statistics
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PSSM-Id: 99738
View PSSM: cd00614
Aligned: 43 rows
Threshold Bit Score: 379.624
Threshold Setting Gi: 31077042
Created: 6-Mar-2002
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site include 1 residue -Click on image for an interactive view with Cn3D
Feature 1:catalytic residue [active site]
Evidence:
  • Comment:pyridoxal 5'-phosphate (PLP) forms an internal aldimine bond (Schiff base linkage) with the catalytic residue lysine
  • Structure:1N8P; catalytic residue (lys) and cofactor (PLP)
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                     
1N8P_A     23 GSVIEPISLSTTFKQSspan----------pigtYEYSRSQNPNRENLERAVAALEn----AQYGLAFSSGSATTATILQ 88  baker's yeast
1CL2_A     22 GAVNSVIQRASSLVFDsveakkha--trnrangeLFYGRRGTLTHFSLQQAMCELEg----GAGCVLFPCGAAAVANSIL 95  Escherichia coli
Q59829     30 AGLPEPVKHEPTLPGPvfaahfhl---pgdptgpYTYGRDENPTWTRLESAIGELEapgeaGVETLVFASGMAAISSVLF 106 Streptomyces coeli...
NP_103124  24 DPIPLPLTMASIFHTPgve------------tgvDQYGRYDNPTWRAVEHVLGHLE-----DAQCVSFPSGMGAISSVFF 86  Mesorhizobium loti
Q07703     28 APVNLPSVRASTVRFQslakledaqrrkaageraSTYGRMGMDTHAALEQVFAELEg----GTHCYLASSGLAGISMVFL 103 Bordetella avium
NP_126586  28 KPLHDPLYITAVFKQVgdaypt-------drgteLKYSREENPTVRKLEDKLAELEg----SDNALAFNSGMSAISTLYF 96  Pyrococcus abyssi
NP_559999  18 GALQPPIYLTALFRMEgealks-------drgfdLKYSREENPTLRPLEEVVANLEg----GVDSLAFNSGMAALSTVFF 86  Pyrobaculum aeroph...
NP_148359  21 EPVVPPVNVSAIYVHPpgsfere-----yprvgdLKYGRENNPTVLGLEEALTSLEe----ARWTLAFNSGMAALSTLVL 91  Aeropyrum pernix
NP_816881  22 HPMVPELVQTSSFYFPtyeefmaa---sideknnYVYTRGTNPTTEILEKKIARLEr----GEKCKVFASGMGAISATLF 94  Enterococcus faeca...
NP_229075  18 KALSFPIFETTNFYFDsfdemska---lrngdyeFVYKRGSNPTTRLVEKKLAALEe----CEDARLVASGMSAISLSIL 90  Thermotoga maritima
Feature 1                                                                                     
1N8P_A     89 SLp-qGSHAVSIg-DVYGGTHRYFTkvanahgVETSFTNdll-ndLPQLIke-nTKLVWIETPTNPTLKVTDIQKVADLI 164 baker's yeast
1CL2_A     96 AFieqGDHVLMTn-TAYEPSQDFCSkilsklgVTTSWFDpligadIVKHLqp-nTKIVFLESPGSITMEVHDVPAIVAAV 173 Escherichia coli
Q59829    107 SQlraGDTAVLPd-DGYQALPLVRAqle-aygIEVRTAPtgr-daQLDVLd--gAKLLWIETPSNPGLDVCDVRRLVEAA 181 Streptomyces coeli...
NP_103124  87 ALlksGDRVLLPa-DGYHATRAMAErflkplgITCDTRPtp--tfLDGGFk--gYSLVFVETPSNPRLDICDISAIAKAV 161 Mesorhizobium loti
Q07703    104 SLlsaGEHALVAd-CAYGPVHELHEavlsrlgIDVTFFDak--adLASLVrp-tTRLIFAEAPGSLLFEMLDMPALARFA 179 Bordetella avium
NP_126586  97 SLlegGDKVVLSm-EGYGTTIELAKllr-kfgVDVKLAYpsa-dsLIETIde-eTKLVLVETMTNPTLKVIDVPEVAKRC 172 Pyrococcus abyssi
NP_559999  87 SLlsaDASVLTPm-EAYGATLRLLNale-kygVRMRKAYpdt-etYIEALkavkPNLAFFETITNPMLRVLDGPEIIKAA 163 Pyrobaculum aeroph...
NP_148359  92 HEvsrGAKKVLLerLTYGSTRSLLEmlssitgIEVRLAGppw-edLLDLVc--wADLIIVESMANPTLRVPPLSGIYREA 168 Aeropyrum pernix
NP_816881  95 TLlqqGDHVLMVn-TIYGESVSFVQyle-kfgVSLTKVDvaeteeLFQFVqe-nTKVIYFESPSSQKFELLDLEKITALA 171 Enterococcus faeca...
NP_229075  91 HFlssGDHVVCVd-EAYSWAKKFFNylskkfdIEVSYVPpda-erIVEAItk-kTKLIYLESPTSMRMKVIDIRKVTEAA 167 Thermotoga maritima
Feature 1                                           #                                         
1N8P_A    165 KkhaagqdVILVVDNTFLSPyisnPLNFGADIVVHSATKYINGHsDVVLGVLATNnkpLYERLQFLqnaigaIPSPFDAW 244 baker's yeast
1CL2_A    174 Rsvv--pdAIIMIDNTWAAGvlfkALDFGIDVSIQAATKYLVGHsDAMIGTAVCNa-rCWEQLRENaylmgqMVDADTAY 250 Escherichia coli
Q59829    182 Hag----gALVAVDNTLATPlgqrPLELGADFSVASGTKQLTGHgDVLLGYVAGRdagAMAAVRRWrkivgaIPGPMEAW 257 Streptomyces coeli...
NP_103124 162 Heq----gGALVVDNTTMTPfgqrPLDFGADIVVSADTKAVNGHsDVLFGHVASRdadIISKVTNWramaggIPGPFEAW 237 Mesorhizobium loti
Q07703    180 Kqh----dLILATDNTWGSGyiyrPLTLGAQVSVIAGTKYVGGHsDLMLGAVVTNdeaIAKRLNRTqyalgySVSADDAW 255 Bordetella avium
NP_126586 173 Rev----gAILVVDNTFVTPllykPLEDGASYVVHSLTKYIAGHnDVLGGAVLWNg-eFSRDLWEWrrrlgtIIQPFDAW 247 Pyrococcus abyssi
NP_559999 164 Kdl----gAVVVVDNTFATPvlttPISYGADVVVHSATKYLAGHnDVVGGIAVVArgdLLEELWIWramlgsIMQPFEAF 239 Pyrobaculum aeroph...
NP_148359 169 Gsc----gVRVVVDNTFATPiayrPLERGAHYSLESLTKYIAGHnDVVGGSLSGRveeDLEPLWNMrkilgtIMQPIDAY 244 Aeropyrum pernix
NP_816881 172 Qki----gAYTVIDNTWASPlfqhPLCHNVDLVIHSCSKYIGGHsDIVAGAVIGKaalVDQIFEHGhqalgaVNSPFNSW 247 Enterococcus faeca...
NP_229075 168 Gel----kIKTVIDNTWASPifqkPKLLGVDVVVHSATKYISGHgDVMAGVIAGDvedMKNIFVDEyknigpVLSPIEAW 243 Thermotoga maritima
Feature 1                                                                                     
1N8P_A    245 LTHRGLKTlhLRVRQAALSANKIAEFLAAdkenvvAVNYpglkthpnYDVVLKqhrdalgGGMISFRikg--gaEAASKF 322 baker's yeast
1CL2_A    251 ITSRGLRTlgVRLRQHHESSLKVAEWLAEhpq-vaRVNHpalpgskgHEFWKRdft--gsSGLFSFVlkkklnnEELANY 327 Escherichia coli
Q59829    258 LAHRSIATlqLRVDRQDSTALKVAEALRTrpe-itGLRYpglpddpsHKVASQqml--ryGCVVSFTlp---srARADRF 331 Streptomyces coeli...
NP_103124 238 LVHRGLETleVRFDRMCSSAETIAQRLKEhra-vrGLRFpglngdpsHNLSRAqme--rfGFLISFEla---seDKAEDF 311 Mesorhizobium loti
Q07703    256 LALRGVRTmpVRMAQHARHALEVCEFLQNrpe-vvRLYHpawpadpgHALWQRdcs--gsNGMLAVQlgl--spQAARDF 330 Bordetella avium
NP_126586 248 MIERGMRTleVRFERQSENAMAIAEFLKDhpk-vrEVHYpglkddpyYKVARRlfkkdlyGGVVAFDlgs--gdNALAFL 324 Pyrococcus abyssi
NP_559999 240 LTLRGLKTlfVRFEKQCKTAMAVAQFLEGhgk-vaEVIYpglsshphHATAKRlfg-dkfGAVVSFRikg--grDAVVRF 315 Pyrobaculum aeroph...
NP_148359 245 LAWRGMKTlkARFEAQSRAAVEVAEWLEShrk-vkKVYYpglsshpdHELARReln-glyGAVVSFEieg--grEEVFKL 320 Aeropyrum pernix
NP_816881 248 LALRGIRTmpVRLAHQSQAIQTVLLALQKdpr-isRLYHpfvgnqqqQALAEKylt--gyGSLFAIDlkd-tdfERLKTF 323 Enterococcus faeca...
NP_229075 244 LILRGLRTleLRMKKHYENALVVSDFLMDhpk-vlEVNYpmnprspqYELASSqms--ggSGLMSFRlkt-dsaEKVKEF 319 Thermotoga maritima
Feature 1                                                                        
1N8P_A    323 ASSTRLFTLAESLGgiESLLEVPavmthggipkeareasgvfddLVRISVGIEDTDDLLEDIKQALK 389 baker's yeast
1CL2_A    328 LDNFSLFSMAYSWGgyESLILANqpehiaairp--qgeidfsgtLIRLHIGLEDVDDLIADLDAGFA 392 Escherichia coli
Q59829    332 LDALRLVEGATSFGgvRSTAERRgrwggd----------avpegFIRLSVGAEDPDDLVADLLRALD 388 Streptomyces coelicolor
NP_103124 312 INNCPLIEAATSFGgvHTSAERRskrgd-----------avppgFVRLSVGCEPVEELWKAIETSLN 367 Mesorhizobium loti
Q07703    331 VNALTLFGIGFSWGgfESLVQLVtpgelarhq----ywqggsdaLVRLHIGLESPADLIADLAQALD 393 Bordetella avium
NP_126586 325 RGLKRIFPSPSLGGv-ESIATYPvksaaktldeeireilgitdgLIRLSVGLEPLDELIEDIDNALG 390 Pyrococcus abyssi
NP_559999 316 FKSLRLITPGPSLGgvESNATYPvasaaspipederkilgitedLVRLSIGLEEAEDLIEDLDRALN 382 Pyrobaculum aerophilum str. IM2
NP_148359 321 LSRLKLVTPSPSFGgvETIISYPaisshsnldplerselgvtdgLLRLSVGLEDVSDIIDDLSQALS 387 Aeropyrum pernix
NP_816881 324 VNALTLVTIGVSWGgfESLALPVfkgnnlaav----qkrglspaHIRMYVGLEEPTSIIEDIQQALD 386 Enterococcus faecalis V583
NP_229075 320 VESLRVFRMAVSWGshENLVVPRvaygdc---------pkkdvnLIRIHVGLGDPEKLVEDLDQALK 377 Thermotoga maritima

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