1RTU,1FUT,1RDS,4GSP,1CH0,1JBS,1DE3


Conserved Protein Domain Family
fungal_RNase
?
cd00606: fungal_RNase 
Click on image for an interactive view with Cn3D
fungal type ribonuclease. Ribonucleases (RNAses) cleave phosphodiester bonds in RNA and are essential for both non-specific RNA degradation and for numerous forms of RNA processing. The members of this CD belong to the superfamily of microbial ribonucleases which are predominantly guanyl specific nucleases. Guanyl specific RNAses are endonucleases which split RNA phosphodiester bonds at the 3' oxygen end of guanosine residues to yield oligonucleotides with the guanosine-2',3'-cyclophosphate at the 3' end and the hydroxyl group at the 5' end. The terminal guanosine-2,3'-cyclophosphate is hydrolysed by guanyl RNAses to give guanosine-3'-phosphate. The alignment also contains ribotoxins, a fungal group of cytotoxins, specifically cleaving the sarcin/ricin loop (SRL) structure of the 23-28S rRNA and therefore being very potent inhibitors of protein synthesis.
Links
?
Statistics
?
PSSM-Id: 238339
Aligned: 10 rows
Threshold Bit Score: 114.466
Created: 6-Mar-2002
Updated: 2-Oct-2020
Structure
?
Program:
Drawing:
Aligned Rows:
Download Cn3D
 
active site
Conserved site includes 5 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1RTU; RNAse U2 with sulphate ion bound at active site
  • Citation:PMID 7492561
  • Structure:1FUT:structure of RNAse F1-inhibitor complex (2'-GMP)
  • Comment:RNAse T1 is very similar to RNAse F1
  • Citation:PMID 8386773
  • Structure:1JBS_A; restrictocin with bound inhibitor that is mimicing target sarcin/ricin loop of 28S rRNA
Scroll to Sequence Alignment Display
Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                      # #                            
1RTU        6 STNCG--------------GNVYSNDDINTAIQGAlddva--ngdrpDNYPHQYYXeasedit----------------- 52  Ustilago sphaerogena
1FUT        3 ATTCG--------------STNYSASQVRAAANAAcqyyqnddtagsSTYPHTYNNyegfd------------------- 49  Gibberella fujikuroi
1RDS        4 EYTCG--------------STCYWSSDVSAAKAKGyslye--sgdtiDDYPHEYHDyegfd------------------- 48  Aspergillus phoenicis
4GSP        3 DYTCG--------------SNCYSSSDVSTAQAAGyklhedgetvgsNSYPHKYNNyegfd------------------- 49  Aspergillus oryzae
1CH0_A      3 DYTCG--------------SNCYSSSDVSTAQAAGyklhedgetvgsNSYPHEFRNwqgfd------------------- 49  Aspergillus oryzae
P07736      5 SVNCG--------------GTYYSSTQVNRAINNAksgq-----yssTGYPHTYNNyegfdf------------------ 47  Ustilago sphaerogena
P09646      3 MYICG--------------SVCYSSSAISAALNKGysyyedgatagsSSYPHRYNNyegfd------------------- 49  Neurospora crassa
P81762      6 SCNCA--------------GRSFTGTDVTNAIRSArag-------gsGNYPHVYNNfegfs------------------- 45  oyster mushroom
1JBS_A      2 TWTCInqqlnpktnkwedkRLLYSQAKAESNSHHAplsd----gktgSSYPHWFTNgydgngklikgrtpikfgkadcdr 77  Aspergillus restri...
1DE3_A      3 TWTCLndqknpktnkyetkRLLYNQNKAESNSHHAplsd----gktgSSYPHWFTNgydgdgklpkgrtpikfgksdcdr 78  Aspergillus giganteus

Feature 1                      #                        #               #             
1RTU       53 --------lccgsgPWSEFPLVY-NGPYYSsrd-nyVSPGPDRVIYQTnTGEFCATVTHTGaasydGFTQCS 114 Ustilago sphaerogena
1FUT       50 ---------fpvdgPYQEFPIKS-GGVYTG------GSPGADRVVINT-NCEYAGAITHTGas-gnNFVGCS 103 Gibberella fujikuroi
1RDS       49 ---------fpvsgTYYEYPIMSdYDVYTG------GSPGADRVIFNG-DDELAGVITHTGas-gdDFVACS 103 Aspergillus phoenicis
4GSP       50 ---------fsvssPYYEWPILSsGDVYSG------GSPGADRVVFNE-NNQLAGVITHTGas-gnNFVECT 104 Aspergillus oryzae
1CH0_A     50 ---------fsvssPYYEWPILSsGDVYSG------GSPGADRVVFNE-NNQLAGVITHTGas-gnNFVECT 104 Aspergillus oryzae
P07736     48 --------sdycdgPYKEYPLKTsSSGYTG------GSPGADRVVYDSnDGTFCGAITHTGas-gnNFVQCS 104 Ustilago sphaerogena
P09646     50 ---------fptakPWYEFPILSsGRVYTG------GSPGADRVIFDS-HGNLDMLITHNGas-gnNFVACN 104 Neurospora crassa
P81762     46 ---------fsctpTFFEFPVFR-GSVYSG------GSPGADRVIYDQ-SGRFCACLTHTGapstnGFVECR 100 oyster mushroom
1JBS_A     78 ppkhsqngmgkddhYLLEFPTFPdGHDYKFdskkpkEDPGPARVIYTYpNKVFCGIVAHQRgn-qgDLRLCS 148 Aspergillus restrictus
1DE3_A     79 ppkhskdgngktdhYLLEFPTFPdGHDYKFdskkpkENPGPARVIYTYpNKVFCGIIAHTKen-qgELKLCS 149 Aspergillus giganteus

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap