1BFD,1QGD,1RP7,1POW,1JSC,2C31,1OZH,1PVD,1DTW,2PDA,1UPC


Conserved Protein Domain Family
TPP_enzymes
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cd00568: TPP_enzymes 
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Thiamine pyrophosphate (TPP) enzyme family, TPP-binding module; found in many key metabolic enzymes which use TPP (also known as thiamine diphosphate) as a cofactor. These enzymes include, among others, the E1 components of the pyruvate, the acetoin and the branched chain alpha-keto acid dehydrogenase complexes.
Links
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Statistics
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PSSM-Id: 238318
Aligned: 132 rows
Threshold Bit Score: 43.013
Created: 7-Mar-2002
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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TPP-binding
Conserved site includes 7 residues -Click on image for an interactive view with Cn3D
Feature 1:TPP-binding site [chemical binding site]
Evidence:
  • Comment:TPP = thiamine pyrophosphate or thiamine diphosphate
  • Structure:1BFD; Pseudomonas putida Benzoylformate Decarboxylase binds TPP and divalent metal ion; contacts at 3.5A
  • Structure:1QGD; Escherichia coli Transketolase binds TPP and divalent metal ion; contacts at 3.5A
  • Structure:1RP7; Escherichia coli Pyruvate Dehydrogenase binds TPP and divalent metal ion; contacts at 3.5A
  • Structure:1JSC; Saccharomyces cerevisiae Acetohydroxyacid Synthase binds TPP and divalent metal ion; contacts at 3.5A
  • Citation:PMID 9665697
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                                                      
1BFD       357 TVFDTLNdma-----------penAIYLNEststtaqmwqrlnm---------------------------rnpgsyyfc 398  Pseudomonas putida
2PDA_A     818 PYVRVITqlf-----------gerMFIANAtgcssiwgasapsmpy-----------------------ktnrlgqgpaw 863  Desulfovibrio af...
P57848      54 ELTVALHyvyk----------tpfDQLIWDvghqayphkiltgrrdqmstirq---------kngihpfpwreesefdvl 114  Pasteurella mult...
NP_126757  370 SYRALLDalrelg------ygrfdIPIHGDigcyalsllp----------------------------------pleaiw 409  Pyrococcus abyss...
CAE78267    69 ELMSALHghvfdvaarenkqwydlFHIVNDaghcenglyalkanykmagltldslkkfrsiesgltghgevhcfpegvfi 148  Bdellovibrio bac...
EAL21013   105 EVVILLSflladq-----dkskyvDILFDEaghrvatqyllsaldghipvehllhy---reansklpghpelgltpgvkf 176  Cryptococcus neo...
EAA61471    69 EVCILLGfinan-------pdafyDAIFDEaghrvatqyllaaidgkiepdhllny---rdansklpghpelgltpgvkf 138  Aspergillus nidu...
YP_064411   80 ELLIVDGfmkgs--------dsivPVYYDEaghrvaiqymmsvlngyndaealfhy---refgkgfyghperderdgiff 148  Desulfotalea psy...
BAC91405    76 MILEALFrgnp---------dkfvPIFFDEaghrvatqylmaalhgelpfeqllqy---raadshlpghpelgltpgvkf 143  Gloeobacter viol...
CAI07892    31 AVLAQIQkllptl-----giprenFAFISGigcssrfpyy-----------------------------------mdtyg 70   Azoarcus sp. EbN1

Feature 1          #                                                                           
1BFD       399 aaggLGFALPAAIGVQLaep------------------------------------------------------------ 418  Pseudomonas putida
2PDA_A     864 gnslFEDAAEYGFGMNMsmfarrthladlaakalesdasgdvkealqgwlagkndpikskeygdklkkllagqkdgllgq 943  Desulfovibrio af...
P57848     115 svghSSTSISAGLGIAVaaqre---------------------------------------------------------- 136  Pasteurella mult...
NP_126757  410 tefvMGASISLANGQSVvm------------------------------------------------------------- 428  Pyrococcus abyss...
CAE78267   149 sngpLGSAFPQTQGVAMgeais---------------------------------------------------------- 170  Bdellovibrio bac...
EAL21013   177 ssgrLGHMWPLVNGVALaek------------------------------------------------------------ 196  Cryptococcus neo...
EAA61471   139 ssgrLGHMWPLVNGIAMahk------------------------------------------------------------ 158  Aspergillus nidu...
YP_064411  149 ssgrLGHMWSHVNGVAEfdr------------------------------------------------------------ 168  Desulfotalea psy...
BAC91405   144 ssgrLGHMWPYVNGVAManp------------------------------------------------------------ 163  Gloeobacter viol...
CAI07892    71 mhsiHGRAPAIASGLKLarp------------------------------------------------------------ 90   Azoarcus sp. EbN1

Feature 1                       ####                                  # #                      
1BFD       419 ---------erQVIAVIGDGSAny----sISALWTAaqy------niPTIFVIMNNGTygalrwfagvle---------- 469  Pseudomonas putida
2PDA_A     944 iaamsdlytkkSVWIFGGDGWAyd---igYGGLDHVlas------geDVNVFVMDTEVysntggqsskatptg------- 1007 Desulfovibrio af...
P57848     137 -------nagrKTVCVIGDGAItag--maFEAMNHAgal------htDMLVILNDNEMsisenvgalnnhlarlltgsfy 201  Pasteurella mult...
NP_126757  429 ---------nkKIVATIGDSTFfh---ngIQPLIDAvyk------dlDVLVIILDNRTtamtghqphpgt---------- 480  Pyrococcus abyss...
CAE78267   171 -------gknrVTVTTISDGACme---geAKEAFAAipglaktgkmgPYVLIISDNNTklsgrida-------------- 226  Bdellovibrio bac...
EAL21013   197 ---------nkAVFILGSDGSQqegddaeAARLAVAqg--------lNVKLFVDDNDVtiaghps--------------- 244  Cryptococcus neo...
EAA61471   159 ---------dkKVFMLGSDGSQq-----eGNDAEAAriav---annlNVKLFLDNNDVtiaghps--------------- 206  Aspergillus nidu...
YP_064411  169 ---------dkTLVMFGSDGSQm-----eGDDAEAAryav---arnlNVKLFIDDNNVtiaghps--------------- 216  Desulfotalea psy...
BAC91405   164 ---------dkVVFCLGSDGSQq-----eGNDAEAArlav---aqglNVKLIVDDNDVtiaghps--------------- 211  Gloeobacter viol...
CAI07892    91 ---------elSVWVVTGDGDAla---igGNHFIHAmrr------nvDLKIILLNNRIygltkgqysptsemg------- 145  Azoarcus sp. EbN1

Feature 1                                                                                      
1BFD       470 ---------------------aenvpgldvpgiDFRALAKg-yGVQALKadn-----------leQLKGSLqeal---sa 513  Pseudomonas putida
2PDA_A    1008 ------------------avakfaaagkrtgkkDLARMVMtygYVYVATvsmg--------yskqQFLKVLkeae---sf 1058 Desulfovibrio af...
P57848     202 ssireggkkilsgmppikefvkkteehvkgfvsPVGTMFEq-lGFNYIGpidg--------hnieELISTLknmr---al 269  Pasteurella mult...
NP_126757  481 ---------------------ggsetgrkfreiDIEALVRa-iGVKYVKtvd-----------pyDLKATKeaikeamqv 527  Pyrococcus abyss...
CAE78267   227 --------------------------esfsmtpTFNSLKDl--GWNVINlaegndlqkcydtivsAIEQAKanp------ 272  Bdellovibrio bac...
EAL21013   245 ---------------------------eylkgySVARTLEg-hGLKVVEan------------geDLDSLYsaivevmnh 284  Cryptococcus neo...
EAA61471   207 ---------------------------vyqkgyELERTLTg-hGMKVVRaq------------geDIDSLYnamvevvst 246  Aspergillus nidu...
YP_064411  217 ---------------------------eymkgyDLNKTLSg-hGLTVSEgd------------geELDGLFariqqaftq 256  Desulfotalea psy...
BAC91405   212 ---------------------------kylpgfSVAKTLAg-hGLAIDEgd------------geDLDGLYarictaita 251  Gloeobacter viol...
CAI07892   146 ------------------kktkstplgsidrpfSPVALALgagATFVARsvdt---------dlpHLASVLaraa---ah 195  Azoarcus sp. EbN1

Feature 1                
1BFD       514 kgPVLIEVST 523  Pseudomonas putida
2PDA_A    1059 pgPSLVIAYA 1068 Desulfovibrio africanus
P57848     270 kgPQFLHIMT 279  Pasteurella multocida
NP_126757  528 kgPAVIIAKR 537  Pyrococcus abyssi GE5
CAE78267   273 iaPVIIHAKT 282  Bdellovibrio bacteriovorus HD100
EAL21013   285 kgPAAVVTHR 294  Cryptococcus neoformans var. neoformans B-3501A
EAA61471   247 dgPAAVVVDR 256  Aspergillus nidulans FGSC A4
YP_064411  257 sgPVAVINKR 266  Desulfotalea psychrophila LSv54
BAC91405   252 agPVAVVNKR 261  Gloeobacter violaceus PCC 7421
CAI07892   196 rgTAFVEILQ 205  Azoarcus sp. EbN1

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