1EGE,1IS2,1IVH,1BUC,3MDE


Conserved Protein Domain Family
ACAD

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cd00567: ACAD 
Click on image for an interactive view with Cn3D
Acyl-CoA dehydrogenase
Both mitochondrial acyl-CoA dehydrogenases (ACAD) and peroxisomal acyl-CoA oxidases (AXO) catalyze the alpha,beta dehydrogenation of the corresponding trans-enoyl-CoA by FAD, which becomes reduced. The reduced form of ACAD is reoxidized in the oxidative half-reaction by electron-transferring flavoprotein (ETF), from which the electrons are transferred to the mitochondrial respiratory chain coupled with ATP synthesis. In contrast, AXO catalyzes a different oxidative half-reaction, in which the reduced FAD is reoxidized by molecular oxygen. The ACAD family includes the eukaryotic beta-oxidation enzymes, short (SCAD), medium (MCAD), long (LCAD) and very-long (VLCAD) chain acyl-CoA dehydrogenases. These enzymes all share high sequence similarity, but differ in their substrate specificities. The ACAD family also includes amino acid catabolism enzymes such as Isovaleryl-CoA dehydrogenase (IVD), short/branched chain acyl-CoA dehydrogenases(SBCAD), Isobutyryl-CoA dehydrogenase (IBDH), glutaryl-CoA deydrogenase (GCD) and Crotonobetainyl-CoA dehydrogenase. The mitochondrial ACAD's are generally homotetramers, except for VLCAD, which is a homodimer. Related enzymes include the SOS adaptive reponse proten aidB, Naphthocyclinone hydroxylase (NcnH), and and Dibenzothiophene (DBT) desulfurization enzyme C (DszC)
Statistics
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PSSM-Id: 173838
View PSSM: cd00567
Aligned: 69 rows
Threshold Bit Score: 122.007
Threshold Setting Gi: 15889795
Created: 7-Mar-2002
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
active site
Conserved site includes 10 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:The catalytic glutamate/aspartate residue can occur at two different positions in ACAD's
  • Comment:Enzymes differ in their specificity for acyl-CoA's of various chain lengths
  • Structure:3MDE_A; Pig MCAD bound to octanoyl-CoA and FAD
    View structure with Cn3D
  • Citation:PMID 3413116

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                     
1EGE_A     18 EQQKEFQATARKfareeiipvaa----------eydktgeypvPLIRRAWELglmnthipencgglglgtfdac--lise 85  human
3MDE_A      8 EQQKEFQATARKfareeiipvaa----------eydrtgeypvPLLKRAWELglmnthipesfgglglgiidsc--lite 75  pig
NP_357176  20 AIAAALAEDFSQtaadhdks---------------gefaagnfEALFKSGLLglvtavkdggwgegmetaha-----vit 79  Agrobacterium tume...
NP_243044  14 TVTKKVASAFQEgarihdqe---------------stfpfahiEALKKIGYPslalprtfggleitllemmk-----hqe 73  Bacillus halodurans
BAC20182   20 EVARELTRKWQAtvverdka---------------ggsateerEDLRASGLLsvtvprhlggwgadwptale-----vvr 79  Bacillus subtilis
BAC90989   39 TRAGQVADFCAAnaarided---------------gavpieefRRLAAAGLLavpldaslggaglgvspgrtla--llsv 101 Gloeobacter violac...
NP_772724  39 RAREIGQSVFAGraatydle---------------atfptenyRDLHRVGLLgiavpkkhgglganyqtyal-----aaa 98  Bradyrhizobium jap...
NP_355476  48 SRVAGIADLFRSsarqteea---------------rrvpashiEALRGIGYFdivkprafngkggdfaelve------an 106 Agrobacterium tume...
NP_069334   9 YNGDHLDEKSREimlktieffenmgkaklkekaskrewyadflEFIKENKIFytlmtppeyadgdpnvrwdtwricdfse 88  Archaeoglobus fulg...
NP_488098  28 SFKQVIDLDEREefph------------------------eiiNWLYNWKLQhyyiptecggeftsfdefia------fv 77  Nostoc sp. PCC 7120
Feature 1                      #                                         # #                  
1EGE_A     86 elaygctgvqtaiegnsLGQMPIIia-----gnDQQKKKYLGRmte-------eplMCAYCVtepgags--dvagikTKA 151 human
3MDE_A     76 elaygctgvqtaieantLGQVPLIig-----gnYQQQKKYLGRmte-------eplMCAYCVtepgags--dvagikTKA 141 pig
NP_357176  80 aiakgdpstalilamhySVHAAIRrgk----wpAALSAKVLVAnnr-------epaLLNNAQaepgvgspahgglpeTTA 148 Agrobacterium tume...
NP_243044  74 tiarfdgatalsigwhvGQIFQWRataq--wkkPEYRLICEQIqnk--------qiLLNSAAtekatgsparggkptTTA 143 Bacillus halodurans
BAC20182   80 eiakvdgslghlfgyhlSTPAVIDlw-----gsPEQSERLLRQlae-------nnwWTGNASsennsh----ilewkVTA 143 Bacillus subtilis
BAC90989  102 leqvgrgnlavgriyegHVNALQLiqi---fgsREQQSACAAAvhg--------gkIYNVWNted---------edgTKI 161 Gloeobacter violac...
NP_772724  99 eigrycgataltwnmhvCSTLWSGpladdldmdAETRAEHERRravhykrivedgaIYSQPFseggaaa-aggvafgTEA 177 Bradyrhizobium jap...
NP_355476 107 ielsaacastgwvagllSAHQWLLam-----fpEEAQADVWGEnpd-------allCGSYAPv--------------KMA 160 Agrobacterium tume...
NP_069334  89 llafysldywytwqvtvLGLGPIWms-----knEKIKKEVAELlkn--------gaVFAFGLserehga-diyatetKLV 154 Archaeoglobus fulg...
NP_488098  78 rvlsrrdqtaaiafttmFWSYLTWma-----gtDEQKRTLASFmkd-------angAMCLAYsekahgs--dllasdVRA 143 Nostoc sp. PCC 7120
Feature 1                    # #                                                              
1EGE_A    152 EKKg-DEYIINGQKMWITNGg-KANWYFLLARSdpdpk----apankAFTGFIVEAdt--pgIQIGrkeln-mgqrcSDT 222 human
3MDE_A    142 EKKg-DEYIINGQKMWITNGg-KANWYFLLARSdpdpk----apaskAFTGFIVEAdt--pgVQIGrkein-mgqrcSDT 212 pig
NP_357176 149 RIEg-DVWVVNGRKSFVTGLp-GLKWAIVLAVTtee---------tpRLIQLLVPLda--agIAQEkawea-tgmyaTAS 214 Agrobacterium tume...
NP_243044 144 TKSg-EEWVIQGRKTFTSMAp-ALDYALVTATIag----------enEVGQFLVPMna--rgVTIDetwnm-vgmraTSS 208 Bacillus halodurans
BAC20182  144 TPTddGGYLLNGIKHFSSGAk-GSDLLLVFGVIpegs------pqqgAIVAAAIPTtr--egVQTNddwqa-lgmrrTDS 213 Bacillus subtilis
BAC90989  162 VPLpgDRYRLEGAKTFASGTglVERPIIGAARPd------------gGWQMVIVPMel--ytGRVDpdwwqplgmraSAS 227 Gloeobacter violac...
NP_772724 178 KPVk-GGWIVNGKKIFASLSg-HADYYGVLCTEiqegg----kasrrNTLYVAIPAks--qgVSVVgdwdp-lgmrgTVS 248 Bradyrhizobium jap...
NP_355476 161 EAAd-GGYRLSGKWAFASGCe-NAQWSLCAAILppkg-------egkPVPAFLLVPas---qYVVEdtwhv-vglagTGS 227 Agrobacterium tume...
NP_069334 155 PQPd-GTYKAYGRKYYIGNSn-VAAIISTFARIadpdgslpdkpdksRFAFFALRRde--kyYELIkniv----daqMFV 226 Archaeoglobus fulg...
NP_488098 144 TKAp-GGYILNGEKWPINRAt-ISGVTFVLAKTdeeg-------garSLSLFMIEKskidpaNYSHlpkifthgvrgSDM 214 Nostoc sp. PCC 7120
Feature 1                                                                                     
1EGE_A    223 RGIVFEDVKVPkenvligdgagfk-------------vamgafdktrpvVAAGAVGLAQRALDEATkyalerkt------ 283 human
3MDE_A    213 RGIVFEDVRVPkenvltgegagfk-------------iamgtfdktrppVAAGAVGLAQRALDEATkyalerkt------ 273 pig
NP_357176 215 HDLVLEDVRVPladivaeqpadepl-----------rrdeadgynffalLASVYHGVALSARDDLLrhltghapv----s 279 Agrobacterium tume...
NP_243044 209 HDLVLDHVRLPlealmeriepg-----------------mkkaagwllhIPACYLGISQAAYDEAIrfakqyspn----t 267 Bacillus halodurans
BAC20182  214 GTTEFHNVVVRpdevlgkpnavveafl-------asgrgslfgpivqlvFATVYLGIARGALETAReytrtqarpwt-pa 285 Bacillus subtilis
BAC90989  228 FKVDFSGIELGedallgvrgdyl---------------rqpwltagvvrFAAVQLGGAAALLDCTRehlrnr-------- 284 Gloeobacter violac...
NP_772724 249 RTLLFEDVFVPedsalmprgvyf--------------qaamrwphmfltLSPTYMGLAQAAYDFTVrylrgevpg----m 310 Bradyrhizobium jap...
NP_355476 228 KTLVLDDVFVPehrvltfpdatsgctpggryyaeeslfnmplltgipscLAAAGVGAAKGALAAYVdhvggrvtrgavag 307 Agrobacterium tume...
NP_069334 227 GDFLIHDYPVTeddilekgeeawd-------------aalntvnvgkfnLGWASIGICTHAFYEAInhaskrrl------ 287 Archaeoglobus fulg...
NP_488098 215 SGIKFENCFIPesmrlgaegagle-------------lalkgfqitralCAAFSQGAADTALRTTLkfalgrql------ 275 Nostoc sp. PCC 7120
Feature 1                                                                                     
1EGE_A    284 fgkllvehQAISFMLAEMAMKVELARMSYQRAAWEVDSgrr------------ntyYASIAKAFAGDIANQLATDAVQIL 351 human
3MDE_A    274 fgkllaehQGISFLLADMAMKVELARLSYQRAAWEIDSgrr------------ntyYASIAKAYAADIANQLATDAVQVF 341 pig
NP_357176 280 lgaplssiPRIQEGLGQIEVLLAANRRLLLSVARDIDAger------------vgtDALAVRHVVIDNAVTVTDLALELG 347 Agrobacterium tume...
NP_243044 268 ldgtistlPHVQQKIGEMAMLLMQSRTILHSVARQWDEssdler-------eemkrELAMAKVTVINNGLSVVDIAMRIV 340 Bacillus halodurans
BAC20182  286 gvtqavedPYTIRSYGEFGIELQAGDAAAREAAQLLQTawdkgdaltpqergelavQVAGVKAIATKAGLDVTSRIFEVI 365 Bacillus subtilis
BAC90989  285 ---gwqehPYQRERMARAAIAVEGGRLWLQGAAERIDRwldnpagp--aggeglvaYANMVRTAVEAACQEVVQLAAKSV 359 Gloeobacter violac...
NP_772724 311 ppvkrrmyPTKQIAVAQMQIKLEQIKSIWFQAVTEARAnpsk----------eqvlRAYAAQYSVMEGANELAALAIRTC 380 Bradyrhizobium jap...
NP_355476 308 gnnrmaefPTIQLRVAEAAASVDAACEILLRDVSRAQAlaqariegraeftvddrlLSRRGQSFAVSLSLRAVQALNDST 387 Agrobacterium tume...
NP_069334 288 ygmyvtdfPHIKRLFVDAYARLMAMKLFARRACDYMRVasred--------rryllYSAMVKSKVTREGEEVINLLWDVI 359 Archaeoglobus fulg...
NP_488098 276 ygktvydmPQPKRTLTDAFLDILICDCATIGVTRGFNTmpeq-----------isvWSAVVKYFVTTNLETVVQNVSVVL 344 Nostoc sp. PCC 7120
Feature 1                                    ##  # #  #        
1EGE_A    352 Ggngfn----teypVEKLMRDAKi-----yQIY--EGTS-QIQRLIVAR 388 human
3MDE_A    342 Ggngfn----teypVEKLMRDAKi-----yQIY--EGTA-QIQRIIIAR 378 pig
NP_357176 348 Gnrglr----rdynLERHHRDAItar-ahaPQS--HMIR-TILGRQSIK 388 Agrobacterium tumefaciens str. C58 (Cereon)
NP_243044 341 Gahslh----mdhdLQRYYRDMRagl--hnPPSedMTIA-MLAKEALRD 382 Bacillus halodurans
BAC20182  366 Gargth----pnygFDRFWRNIRtht-lhdPVS--YKIA-EVGNYVLNQ 406 Bacillus subtilis
BAC90989  360 Garcll----rphpVERIVRDLTlyl-rqpAPD--AALA-QLGTYVIEQ 400 Gloeobacter violaceus PCC 7421
NP_772724 381 Ggqaml----rslpLERIYRDSRcgslmlpWTA--ELCL-DRIGREALY 422 Bradyrhizobium japonicum USDA 110
NP_355476 388 Ggvgld----lanpVQRAWRDANavg-rhiSMNw-DAVG-TMIGQSMLG 429 Agrobacterium tumefaciens str. C58 (Cereon)
NP_069334 360 Aakgfe----kdtyFEMAARHIRa-----lPKL--EGTV-HVNMALILK 396 Archaeoglobus fulgidus DSM 4304
NP_488098 345 GsrfymreehdygiFQKVLRDNAi-----iSMF--DGSTvVNLHALLLQ 386 Nostoc sp. PCC 7120

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