1GEF,1HH1,1OB8,2WIZ,2WJ0,2WJ0


Conserved Protein Domain Family
Holliday_junction_resolvase

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cd00523: Holliday_junction_resolvase 
Click on image for an interactive view with Cn3D
Holliday junction resolvase
Holliday junction resolvases (HJRs) are endonucleases that specifically resolve Holliday junction DNA intermediates during homologous recombination. HJRs occur in archaea, bacteria, and in the mitochondria of certain eukaryotes; however, this CD includes only the archeal HJRs. The bacterial and archeal HJRs perform a similar function but differ in both sequence and structure. Structural similarity does however, exist between the archeal HJRs and type II restriction endonucleases, such as EcoRV, BglII, and Fok, and this similarity includes their active site configurations.
Statistics
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PSSM-Id: 411703
Aligned: 17 rows
Threshold Bit Score: 123.216
Created: 7-Mar-2002
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 4 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:2WJ0: Archaeoglobus fulgidus Holliday junction resolvase active site binds DNA substrate and divalent cation; contacts at 4A
    View structure with Cn3D
  • Structure:2WIZ: Archaeoglobus fulgidus Holliday junction resolvase active site contacts substrate DNA; contacts at 4A
    View structure with Cn3D
  • Comment:consensus E-X(1,)-VD-X(1,)-EXK binds Mg2+

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                #                              #             # #                        
1GEF_A         1 MYRKGAQAERELIKLLEKHGFAVVRSAGSK-------KVDLVAGNGk-kYLCIEVKVTKK--DHLYVGKRDMGRLIEFSR 70  Pyrococcus furi...
1HH1_A         4 KKRKGSAVERNIVSRLRDKGFAVVRAPASGskr-kdpIPDIIALKNg-vIILIEMKSRKDieGKIYVRREQAEGIIEFAR 81  Sulfolobus solf...
1OB8_A         2 NRDIGKNAERELVSILRGEGFNAVRIPTSNssp--npLPDIFATKGn-tLLSIECKSTWE--NKVKVKEHQVRKLLDFLS 76  Sulfolobus solf...
2WJ0_A         4 GKSKGTRFERDLLVELWKAGFAAIRVAGSGvsp--fpCPDIVAGNGr-tYLAIEVKMRKE--LPLYLSADEVEQLVTFAR 78  Archaeoglobus f...
Q57920         4 KYRKGSSFERELKRLLEKEGFAVIRSAGSK-------GVDLIAGRKg-eVLIFECKTSSK--TKFYINKEDIEKLISFSE 73  Methanocaldococ...
BAA79424       3 MPRRGVGYERELAKILWERGWAVIRGPASGggsrsrvQPDLVAVRGg-vVLVFEIKKARG--ETVYLDPGQVLGLLEWAR 79  Aeropyrum perni...
Q5JGD9         2 RYRRGASAERELVKLLESKGFAVLRSAGSH-------KIDLVAGNGk-eYLCIEVKSTRS--RKLYLPIEDVEKLVEFAG 71  Thermococcus ko...
ABO35636       4 KYQKGSAFERELKKKLENHGFAVIRSAGSH-------GVDLVAGKKgkkPIVIECKSTSK--DKYYIPNEDVEKLLEFSE 74  Methanococcus m...
ACL10961       3 NRSRGFSHERDLVRRLWDHGFAVIRAPASGsrarhvkYPDIVAIYHg-kIVAMEVKTIKEe-RTIYVRREQVEKLQEFSR 80  Desulfurococcus...
WP_011008462   3 VKRKGSAKERELANFFWERGCAVLRGCSSGggvrkryVPDVVAICKg-kVLVFEVKYRSKy-TPIKIEREKLEKLAVFAK 80  Pyrobaculum aer...
Feature 1                                                                
1GEF_A        71 RF--GGIPVLAVKFLn-VGWRFIEVSpkiekf--------vftpsSGVSLEVLLGI 115 Pyrococcus furiosus
1HH1_A        82 KS--GGSLFLGVKKP--GVLKFIPFEklrrtetg---nyvadseiEGLDLEDLVRL 130 Sulfolobus solfataricus
1OB8_A        77 MFtmKGVPLIAIKFKqvHEWRVLVPEkaedi---------ivtidNSIPIEDLFKI 123 Sulfolobus solfataricus
2WJ0_A        79 GF--GAEAYVALKLPr-KKWRFFPVQmlerteknf--kidesvypLGLEIAEVAGK 129 Archaeoglobus fulgidus
Q57920        74 IF--GGKPYLAIKFN--GEMLFINPFllstngkny--viderikaIAIDFYEVIGR 123 Methanocaldococcus jannaschii DSM 2661
BAA79424      80 RA--GGDAWIALRLVg-KGWRFHRADslehtrrggfkisrpggglKLRDLLTLYGG 132 Aeropyrum pernix K1
Q5JGD9        72 RF--GGRPVLAVKFVn-VGWRFYGPNrleh-----------geksYKIDLETPFMT 113 Thermococcus kodakarensis KOD1
ABO35636      75 TF--DGIPYIALKIN--RKCLFINPYlltsagkny-aldyeklcpIALDIKDVAED 125 Methanococcus maripaludis C5
ACL10961      81 RA--GATPFIAVKFVgsGEWFLIPLEklagseggt-fkipvetvkNSLRLKALISM 133 Desulfurococcus kamchatkensis 1221n
WP_011008462  81 RA--GGEAYLLVKYGr-NPWKVLEIR-------------------DKIDREEYEKA 114 Pyrobaculum aerophilum str. IM2

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