1JEE,1I2D,1JHD,2GKS,1X6V,1V47,3CR8


Conserved Protein Domain Family
ATPS

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cd00517: ATPS 
Click on image for an interactive view with Cn3D
ATP-sulfurylase
ATP-sulfurylase (ATPS), also known as sulfate adenylate transferase, catalyzes the transfer of an adenylyl group from ATP to sulfate, forming adenosine 5'-phosphosulfate (APS). This reaction is generally accompanied by a further reaction, catalyzed by APS kinase, in which APS is phosphorylated to yield 3'-phospho-APS (PAPS). In some organisms the APS kinase is a separate protein, while in others it is incorporated with ATP sulfurylase in a bifunctional enzyme that catalyzes both reactions. In bifunctional proteins, the domain that performs the kinase activity can be attached at the N-terminal end of the sulfurylase unit or at the C-terminal end, depending on the organism. While the reaction is ubiquitous among organisms, the physiological role of the reaction varies. In some organisms it is used to generate APS from sulfate and ATP, while in others it proceeds in the opposite direction to generate ATP from APS and pyrophosphate. ATP sulfurylase can be a monomer, a homodimer, or a homo-oligomer, depending on the organism. ATPS belongs to a large superfamily of nucleotidyltransferases that includes pantothenate synthetase (PanC), phosphopantetheine adenylyltransferase (PPAT), and the amino-acyl tRNA synthetases. The enzymes of this family are structurally similar and share a dinucleotide-binding domain.
Statistics
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PSSM-Id: 173895
View PSSM: cd00517
Aligned: 29 rows
Threshold Bit Score: 393.922
Threshold Setting Gi: 223673943
Created: 7-Mar-2002
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 16 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1I2D_A; Penicillium chrysogenum ATPS binds APS, defined using 4.0 A contacts
    View structure with Cn3D
  • Structure:1JEE_A; Saccharomyces cerevisiae ATPS binds APS analog, defined using 4.0 A contacts
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                     
1JEE_A     31 ILVWNLTpRQLCDIELIlNGGFSPLTGFLNENDYSSVVTDSRLadg------tlWTIPITLDVDeafanq--ikpdtRIA 102 baker's yeast
1I2D_A     31 LPAVTLTeRQLCDLELImNGGFSPLEGFMNQADYDRVCEDNRLadg------nvFSMPITLDASqevidekklqagsRIT 104 Penicillium chryso...
2GKS_A      7 LKSIQISqRSVLDLELLaVGAFTPLDRFMGEEDYRNVVESMRLksg------tlFPIPITLPMEkeiakd--lkegeWIV 78  Aquifex aeolicus
1336213    82 NKRIELS-DRTCDVELLtVGAFSPLEGFMNKAEYDSVVANMRMtng------llFGLPIVLDTDsedi-----vpgdKLL 149 Chlamydomonas rein...
7388234    35 LPRVQLDeRATSDLVMIaIGGFSPLKGFMEQDDYELVVEEMKLsng------lpWSVPVTLSVTeevaap--levgsWVR 106 Synechocystis sp. ...
7388238    25 ACQAELDqLALSDLELIaIGGYSPLTGFLGEKDYHSVVKEMRLang------lpWSLPITLPVGektarq--lsagdHVK 96  Bacillus subtilis
AAL92174   31 LNDIFLTeRQLCDLELIlNGGFSPLEGFMDEQDYTSVVETLRLapfnghkyghvFPIPITLDVSqedintlglkqgaRVA 110 Cryptococcus neofo...
P78937     27 LPKIVLSeRQFCDVELIlNGGFSPLDGFMNQKDYLNVVENLRLstg------evFPIPITLDLNesqads--lkagdRVA 98  fission yeast
AAP97122   28 MPKIVVNgTDYTTLHRIaDGTLSPLTGPMTKTDYDNVLVKKGIerdg---kmwaWTIPLSLPVTaeeaks--lkagePAL 102 Porphyra purpurea
NP_896399  40 TKSLECSdRNACDVELLvVGGFSPLRGFMHQEDYDAVVSGHRLvag------qlFGLPIVMDTDredv-----vvgdTVL 108 Synechococcus sp. ...
Feature 1                                                                                     
1JEE_A    103 LFQdd--eIPIAILTVQDVYKPnktieaekvfrgd-pehpaisylfnvaGDYYVGGSLEAIQLpqhydypgLRKTPAQLR 179 baker's yeast
1I2D_A    105 LRDfr-ddRNLAILTIDDIYRPdktkeaklvfggd-pehpaivylnntvKEFYIGGKIEAVNKlnhydyvaLRYTPAELR 182 Penicillium chryso...
2GKS_A     79 LRDpk--nVPLAIMRVEEVYKWnleyeaknvlgtt-dprhplvaemhtwGEYYISGELKVIQLpkyydfpeYRKTPKQVR 155 Aquifex aeolicus
1336213   150 LTYq---gQDLAVMTVDSKFTPnkpleclkcygtsslehpavqmvamerGKYYIGGPIKGLALptr---vfPCASPADVR 223 Chlamydomonas rein...
7388234   107 LDNsa--gKFIGVLELTQKYHYnkaheaknvyrtd-dqahpgvkviydqGPVNLAGPIWLLERephplfpkYQIDPAASR 183 Synechocystis sp. ...
7388238    97 LVKd---gVTYGMITVTDIYQPdktqealsvfktn-dpahpgvkkllarPDYYIGGPITVSSLpdks-feqFYATPAETR 171 Bacillus subtilis
AAL92174  111 LRDpr-ddAALAILTVSDIYRPnkateaekvmgaddiahpsvaylrnnvKEFYVGGKVQAIQApthfdyvpLRYTPAELR 189 Cryptococcus neofo...
P78937     99 LLDprdgqTVIAILTVEDKYTPdkaneaekvfgandrahpavdylfgraGNVYVGGKLQAVTPirhfdfveYRYSPAQLR 178 fission yeast
AAP97122  103 LVNea--gDAFGTLDVDATFSWdkpaflkavygtdrtdhpgarlwlddaRVDLVGGAISMLAHdenrpfkdRIMYPTSMR 180 Porphyra purpurea
NP_896399 109 LTYk---gQNLAVLQVEAKWEPnkvveakgcygttsiehpavrmitmerKRFYLGGSLNGLELpqr---vfPCKTPAEVR 182 Synechococcus sp. ...
Feature 1                  #####     #  #                                                     
1JEE_A    180 LEFQSRqwdRVVAFQTRNPMHRAHRELTVRAAREan--------aKVLIHPVVGLTKPGDIDHHTRVRVYQEIIKRYP-- 249 baker's yeast
1I2D_A    183 VHFDKLgwsRVVAFQTRNPMHRAHRELTVRAARSrq--------aNVLIHPVVGLTKPGDIDHFTRVRAYQALLPRYP-- 252 Penicillium chryso...
2GKS_A    156 EEIKSLgldKIVAFQTRNPMHRVHEELTKRAMEKvg--------gGLLLHPVVGLTKPGDVDVYTRMRIYKVLYEKYYd- 226 Aquifex aeolicus
1336213   224 STLPANq--DVLAFQCRNPIHKAHYELFIRALDRnvrn----pgaVCLVHPTCGPTQDDDIPGVVRFRTYEVLKEETK-- 295 Chlamydomonas rein...
7388234   184 QLFAERgwkTIVGFQTRNPIHRAHEYIQKCALEVv---------dGLFLHPLVGATKSDDIPADVRMRCYEIMVDNYFp- 253 Synechocystis sp. ...
7388238   172 AAFQKLgwkTIVGFQTRNPVHRAHEYIQKTALETv---------dGLLLHPLVGETKSDDIPSDIRMESYQALLNHYYp- 241 Bacillus subtilis
AAL92174  190 AHFHKLawrKVVAFQTRNPMHRAHRELTVRAARQrr--------aNVLIHPVVGLTKPGDVDHYTRVRAYQALMPSYP-- 259 Cryptococcus neofo...
P78937    179 SDFQRNnwnRVVAFQTRNPMHRAHRELTVRAAKQhg--------aRVLIHPVVGMTKPGDIDHFTRVRVYEAILQRYP-- 248 fission yeast
AAP97122  181 ALLQEQgygASVAFQTRNPLHRAHEYALVYGAEKllkevgdtakvGVFLNPLVGQLKGDDVPAATRMETYIKLIDGGVlg 260 Porphyra purpurea
NP_896399 183 AGLPDGe--DVVAFQCRNPIHRAHYELFTRALHAqnvs----enaVVLVHPTCGPTQQDDIPGAVRFQTYERLAAEVN-- 254 Synechococcus sp. ...
Feature 1                                 #                        ### ##                     
1JEE_A    250 ----------------NGIAFLSLLPLAMRMSGDREAVWHAIIRKNYGASHFIVGRDHAGPGknskgvDFYGPYDAQELV 313 baker's yeast
1I2D_A    253 ----------------NGMAVLGLLGLAMRMGGPREAIWHAIIRKNHGATHFIVGRDHAGPGsnskgeDFYGPYDAQHAV 316 Penicillium chryso...
2GKS_A    227 ----------------KKKTILAFLPLAMRMAGPREALWHGIIRRNYGATHFIVGRDHASPGkdskgkPFYDPYEAQELF 290 Aquifex aeolicus
1336213   296 ----------------NPRLRWAYLPYSMHMAGPREAIQHMIIRKNYGCTHFIIGRDMAGCKssisgqDFYRAYDAQDLA 359 Chlamydomonas rein...
7388234   254 ----------------KERVILGINPSAMRYAGPREAIFHALIRKNYGCTHFIVGRDHAGVG------DYYGTYDAQEIF 311 Synechocystis sp. ...
7388238   242 ----------------KDRVMLSVFPAAMRYAGPREAIFHALVRKNYGCTHFIVGRDHAGVG------SYYGTYDAQNIF 299 Bacillus subtilis
AAL92174  260 ----------------EGMAHLALLPLAMRMAGPREAVWHAVIRKNFGATHFIVGRDHAGPGknsqgkDFYGPYDAQELV 323 Cryptococcus neofo...
P78937    249 ----------------KGSAKLSLLPLAMRMAGPREALWHAIIRKNYGASHFIIGRDHAGPGknsqgeDFYGPYDAQYLV 312 fission yeast
AAP97122  261 egdmdealwkskgqslREQVRLAGLHMRMFYGGPSEAVMHAIYRQNMGITHFIIGRKHADAPydd-ksAIWGDFDAQEIF 339 Porphyra purpurea
NP_896399 255 ----------------NDRIRWAYLPYAMHMAGPREALQHMIIRRNYGCTHFIIGRDMAGCKssltgdDFYGPYDAQNFA 318 Synechococcus sp. ...
Feature 1                    ###                                                         
1JEE_A    314 ESYkhe-ldIEVVPFRMVTYLPdedRYAPIDqidttktRTLNISGTELRRRLR-VGGEIPEWFSYPEVVKILRES 386 baker's yeast
1I2D_A    317 EKYkde-lgIEVVEFQMVTYLPdtdEYRPVDqvp-agvKTLNISGTELRRRLR-SGAHIPEWFSYPEVVKILRES 388 Penicillium chrysogenum
2GKS_A    291 KKYede-igIKMVPFEELVYVPeldQYVEINeakkrnlKYINISGTEIRENFLkQGRKLPEWFTRPEVAEILAET 364 Aquifex aeolicus
1336213   360 NKHaae-lnMQTVASLNIAYTEe-kGYVTADiakaenlHVLNLSGTKFRQMLR-AGDDIPEWFAFKSVVAVLREQ 431 Chlamydomonas reinhardtii
7388234   312 DEFapealgIVPMKFEHAFYCKktlQMATTKtspsgpeDRIHLSGTKVRALLR-DGQLPPPEFSRPEVAQELIRA 385 Synechocystis sp. PCC 6803
7388238   300 QSFteeelgIKPLFFEHSFYCRkcgNMGTSKtcphsprDHIHLSGTKVRELLR-QGKKPPKEFSRPEVAAVLIKG 373 Bacillus subtilis
AAL92174  324 TQFkde-lqIEMVPFQAMTYLPgsdEYQPVDevp-kgtPTADISGTELRKRLR-TGASIPDWFSYTGVVKVLRES 395 Cryptococcus neoformans...
P78937    313 EQYaqe-igITIVPFQMMTYLPdedIYKPVDkve-pgtRTLNISGTELRRRLR-VGANIPEWFSYPEVVAILRQS 384 fission yeast
AAP97122  340 EKLegd-lqIKTVNVGFAAYFEe-lGKVSLCsen-kgkTTVSISGSKMREMLN-SGTMPDSRVMRPATAQVLMDY 410 Porphyra purpurea
NP_896399 319 KECape-ltMETVPSLNLVYTEe-eGYVTAEhaearglHVKKLSGTQFRKMLR-GGEEIPEWFAFKSVVEVLRAA 390 Synechococcus sp. WH 8102

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