1H7O,1GZG,1YLV,1I8J,1B4K,1W1Z,1B4E,1L6S


Conserved Protein Domain Family
ALAD_PBGS
?
cd00384: ALAD_PBGS 
Click on image for an interactive view with Cn3D
Porphobilinogen synthase (PBGS), which is also called delta-aminolevulinic acid dehydratase (ALAD), catalyzes the condensation of two 5-aminolevulinic acid (ALA) molecules to form the pyrrole porphobilinogen (PBG), which is the second step in the biosynthesis of tetrapyrroles, such as heme, vitamin B12 and chlorophyll. This reaction involves the formation of a Schiff base link between the substrate and the enzyme. PBGSs are metalloenzymes, some of which have a second, allosteric metal binding site, beside the metal ion binding site in their active site. Although PBGS is a family of homologous enzymes, its metal ion utilization at catalytic site varies between zinc and magnesium and/or potassium. PBGS can be classified into two groups based on differences in their active site metal binding site. They either contain a cysteine-rich zinc binding site (consensus DXCXCX(Y/F)X3G(H/Q)CG) or an aspartate-rich magnesium binding site (consensus DXALDX(Y/F)X3G(H/Q)DG). The cysteine-rich zinc binding site appears more common. Most members represented by this model also have a second allosteric magnesium binding site (consensus RX~164DX~65EXXXD, missing in a eukaryotic subfamily with cysteine-rich zinc binding site).
Links
?
Statistics
?
PSSM-Id: 238226
Aligned: 73 rows
Threshold Bit Score: 454.259
Created: 6-Mar-2002
Updated: 2-Oct-2020
Structure
?
Program:
Drawing:
Aligned Rows:
Download Cn3D
 
Conserved site includes 16 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:The active site of ALAD contains A-site and P-site, each binding one molecule of 5-aminolevulinic acid (ALA); the A-site contains the metal ion binding site
  • Structure:1H7O, Saccharomyces cerevisiae ALAD containing a cysteine-rich zinc binding site with bound 5-aminolevulinic acid
  • Structure:1GZG; Pseudomonas porphobilinogen synthase containing an aspartate-rich metal binding site with bound substrate 5-fluorolevulinic acid
Scroll to Sequence Alignment Display
Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                                                       
1H7O_A       19 AGGYNHPLLRQWQseRQLTKNMLIFPLFISdnpdDFTEIDSLPNINRIGv-nRLKDYLKPLVAKGLRSVILFGVPlipgt 97  Saccharomyces ce...
1YLV_A       19 AGGYNHPLLRQWQseRQLTKNMLIFPLFISdnpdDFTEIDSLPNINRIGv-nRLKDYLKPLVAKGLRSVILFGVPlipgt 97  baker's yeast
NP_789665     8 RRLRLSEAMRRLAseHVLVPHKLVLPIFVGeg-lQPRPIPSMPGVVQHSi-dSFKRELQKAACAKLGGVMLFGIPdsrse 85  Tropheryma whipp...
NP_341737    15 RRLRKSKLVRDLVseTSISQNDLVLPVFIKeninEPEQIASMPGIYRYPpndKLINYLQDSYENGIRSVILFGIPsykdd 94  Sulfolobus solfa...
NP_394415     8 RRYRKDQNFRDLFseTSIRPEKLVMPIFVEegisKPVEISSMPGILRYPl-gDLKAYVKHLEDIGVKSVLLFGVPshkds 86  Thermoplasma aci...
YP_055017    30 RRLRTTPAMRRMVaeTRVAPAQLMLPAFVKegidSPVEITSLPGQFQHSt-eSIKELATQAAEAGIGGIDLFGIPehkde 108 Propionibacteriu...
YP_024089     8 RRYRINENIRDIFkeTVIRTEKLIMPVFVDetekDKREIDSMPGIYRYSl-dSYEDYIKYLEEIGVKNVILFGIPahkds 86  Picrophilus torr...
ZP_00884571   7 RRLRQSKALRELFyeTRLYSKEFIFPLFVDdgknVFERMPQLDGIVKVSv-dRLHEILDEIKKVDIGGVILFGVTsqkde 85  Caldicellulosiru...
P77923       14 RRLRRTPAIRRMVaeTRLDPADLVLPMFAVegltEPREIATMPGVWQHTk-eSLQKAVHDAADAGVGAVMLFGTPlerdp 92  Propionibacteriu...
EAX12399     80 RRLRSTVAMRRLVaqTSLEPRHLVLPMFVAdgidEPRPITSMPGVVQHTr-dSLRRAAAAAVAAGVGGLMLFGVPrdqdk 158 Mycobacterium tu...

Feature 1                                         # # #       #                                 
1H7O_A       98 kdpvgtaaddpaGPVIQGIKFIREYfp-eLYIICDVCLCEYTSHGHCGVLyddg---tinRERSVSRLAAVAVNYAKAGA 173 Saccharomyces ce...
1YLV_A       98 kdpvgtaaddpaGPVIQGIKFIREKfp-eLYIICDVCLCEYTSHGHCGVLyddg---tinRERSVSRLAAVAVNYAKAGA 173 baker's yeast
NP_789665    86 ga---edafrpdGILNKACSIAVREvgdsLLIQTDLCLDEFTQHGHCGVVskdg---sidNDATLEMYAEMALAQAAAGS 159 Tropheryma whipp...
NP_341737    95 ia---ssaydknGIIQRAVRIIKDSfkdkIIVITDECTDEYMANGHCGLVkytngcyvvdNDESLKIHAKIALSQAEAGA 171 Sulfolobus solfa...
NP_394415    87 mg---saaydknGVIQKAISVIKDNt--nIITIADLCLCEYTDTGQCGLLkdg----yvdNDSTLEVYRKIAKSYAEAGV 157 Thermoplasma aci...
YP_055017   109 ig---sqawdlkGILNAGISAVREAvgddLVICADTCLDEFTDHGHCGALtpdg---rvdNDSTVPLYAAMAVSQARAGA 182 Propionibacteriu...
YP_024089    87 tg---sssydrnGIIQRAIRIAKDNt--rLNVIADLCLCEYTDTGHCGIIkdn----yidNDATLSIYQKEALSYADAGV 157 Picrophilus torr...
ZP_00884571  86 mg---syatkddGAVQQAIRKIKEYse-dILVFADVCLCEYTSHGHCGILqgd----kidNDKTIEVLSEIALSYAKAGA 157 Caldicellulosiru...
P77923       93 ig---sqawnphGILANAVRWAVEAep-eLPVIADVCLDEFTDHGHCGVLapdg---tvdNDATLPLYAKMSVVLADAGA 165 Propionibacteriu...
EAX12399    159 dgv-gsagidpdGILNVALRDLAKDlgeaTVLMADTCLDEFTDHGHCGVLddrg---rvdNDATVARYVELAVAQAESGA 234 Mycobacterium tu...

Feature 1            #                              #     #  ##    #      #   #                 
1H7O_A      174 HCVAPSDMIDGRIRDIKRGLINANlahKTFVLSYAAKFSGNLYGPFRDAacSApsnGDRkcYQLPpagrgLARRALERDM 253 Saccharomyces ce...
1YLV_A      174 HCVAPSDMIDGRIRDIKRGLINANlahKTFVLSYAAKFSGNLYGPFRDAacSApsnGDRkcYQLPpagrgLARRALERDM 253 baker's yeast
NP_789665   160 QMLGLSGCMDGQTGFVREVLDSNGn-hDVSLVAYSAKYASALYGPFRHAtnSPf-sGSRktYQLNpanlrEAIRKIEVDI 237 Tropheryma whipp...
NP_341737   172 DVIAPSSMMDGVVRAIRDALDSAGy-iDTLILSYSVKYASVMYGPFRDAaySKpafGDRrgYQMDprnafEAIKEARLDI 250 Sulfolobus solfa...
NP_394415   158 DIVAPSGMMDGQVSAIRDELDNDGf-eNVMIMAYSSKFSSNLYGPFREAaeSApkvGDRksYQMDyrnqrEALREIDLDV 236 Thermoplasma aci...
YP_055017   183 HMVSPSGMMDGQIAVIRDTLDREGf-tDVSIMAYSAKYASAFFGPFRDAvnCSl-kGDRktYQQDppnrrEGLRETLLDL 260 Propionibacteriu...
YP_024089   158 DIIAPSGMMDGQVKAIREILDKNDy-kNTIIMAYSAKFASNLYGPFRDAadSTpqfGDRksYQMDyhngnEAIREVDLDV 236 Picrophilus torr...
ZP_00884571 158 DIICPSDMMDGRVDAIRKKLDSHGf-vYTPIIPYSAKFASSLYAPFRDVanSRpafGDRksYQMPyqnkrEALREIEADI 236 Caldicellulosiru...
P77923      166 AMLGPSGMMDGQIAVIRKALDDAGh-tDTVLMAYSAKYASGFFGPFREAvdSQl-kGDRraYQQDpanaiESLREIELDL 243 Propionibacteriu...
EAX12399    235 HVVGPSGMMDGQVAAIRDGLDAAGy-iDVVILAYAAKFASAFYGPFREAvsSSl-sGDRrtYQQEpgnaaEALREIELDL 312 Mycobacterium tu...

Feature 1                #                       #  #                                      #    
1H7O_A      254 SEGADGIIVKPSTFYLDIMRDASEickdLPICAYHVSGEYAMLHAAAekgvvdlkTIAFESHQGFLRAGARLIITYLAPE 333 Saccharomyces ce...
1YLV_A      254 SEGADGIIVKPSTFYLDIMRDASEickdLPICAYHVSGEYAMLHAAAekgvvdlkTIAFESHTGFLRAGARLIITYLAPE 333 baker's yeast
NP_789665   238 SEGADALIVKPAGYYLDVLRCASEis-rIPVMAYQVSGEYAMIKSAAawt--dtkQLVIESLLSIHRAGADAILTYWALD 314 Tropheryma whipp...
NP_341737   251 EEGADILMVKPAHTYLDVIRLVKDhfpeYPLAAYHVSGEYSMIKAAAingwidekTAVLEITTAIKRAGADLIITYYAND 330 Sulfolobus solfa...
NP_394415   237 YEGADIIMVKPAIFYLDIIAKARErf-dLPLAAYSVSGEYNMIYNAVkngy-lseDAIRESLVSIFRAGADIVITYFTEQ 314 Thermoplasma aci...
YP_055017   261 AEGADLVMVKPASHYLDVLSDVAEvs-qVPVAAYQVSGEYAMLEAAAangwidrrRCIGESLTSIVRAGADLVLTYWAIE 339 Propionibacteriu...
YP_024089   237 KEGADIVMVKPGLFYLDIINRVKSiy-kMPVATYSVSGEYSMIKNAIksgl-lnaDARDEALTALFRAGSDLVITYFAEE 314 Picrophilus torr...
ZP_00884571 237 LEGADAVIIKPALTSLDVISAAKEkf-nIPIIAYNVSGEYAMVKSAGklgllneeEVIIEILTAIKRAGADAIITYHALE 315 Caldicellulosiru...
P77923      244 EQGADFVMVKPAMAYLDVLAAARAis-nVPVAAYVVSGEYSMIEFAAkagaldrkRCIMEALTSVKRAGASTIVTYWATE 322 Propionibacteriu...
EAX12399    313 DEGADIVMVKPAMGYLDVVAAAADvs-pVPVAAYQVSGEYAMIRAAAannwiderAAVLESLTGIRRAGADIVLTYWAVD 391 Mycobacterium tu...

Feature 1            
1H7O_A      334 FLDWL 338 Saccharomyces cerevisiae
1YLV_A      334 FLDWL 338 baker's yeast
NP_789665   315 AASWL 319 Tropheryma whipplei TW08/27
NP_341737   331 IARWI 335 Sulfolobus solfataricus P2
NP_394415   315 ILTNS 319 Thermoplasma acidophilum DSM 1728
YP_055017   340 AAQMF 344 Propionibacterium acnes KPA171202
YP_024089   315 YIKDH 319 Picrophilus torridus DSM 9790
ZP_00884571 316 AARIL 320 Caldicellulosiruptor saccharolyticus DSM 8903
P77923      323 VAIWL 327 Propionibacterium freudenreichii subsp. freudenreichii
EAX12399    392 AAGWL 396 Mycobacterium tuberculosis F11

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap