1QAX,1QAX,1QAY,1QAY,1DQA


Conserved Protein Domain Family
HMG-CoA_reductase

?
cd00365: HMG-CoA_reductase 
Click on image for an interactive view with Cn3D
Hydroxymethylglutaryl-coenzyme A (HMG-CoA) reductase (HMGR)
Hydroxymethylglutaryl-coenzyme A (HMG-CoA) reductase (HMGR) is a tightly regulated enzyme, which catalyzes the synthesis of coenzyme A and mevalonate in isoprenoid synthesis. In mammals, this is the rate limiting committed step in cholesterol biosynthesis. Bacteria, such as Pseudomonas mevalonii, which rely solely on mevalonate for their carbon source, catalyze the reverse reaction, using an NAD-dependent HMGR to deacetylate mevalonate into 3-hydroxy-3-methylglutaryl-CoA. There are two classes of HMGR: class I enzymes which are found predominantly in eukaryotes and contain N-terminal membrane regions and class II enzymes which are found primarily in prokaryotes and are soluble as they lack the membrane region. With the exception of Archaeoglobus fulgidus, most archeae are assigned to class I, based on sequence similarity of the active site, even though they lack membrane regions. Yeast and human HMGR are divergent in their N-terminal regions, but are conserved in their active site. In contrast, human and bacterial HMGR differ in their active site architecture. While the prokaryotic enzyme is a homodimer, the eukaryotic enzyme is a homotetramer.
Statistics
?
PSSM-Id: 153080
View PSSM: cd00365
Aligned: 6 rows
Threshold Bit Score: 472.933
Threshold Setting Gi: 7245361
Created: 6-Mar-2002
Updated: 2-Oct-2020
Structure
?
Program:
Drawing:
Aligned Rows:
 
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1:catalytic residues [active site]
Evidence:
  • Comment:In addition to the conserved Glu, Asp, and His, all HMGR contain a catalytic Lys whose position differs between enzyme classes

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                     
1QAX_A      8 PAFRNLsp--aARLDHIGqll---glsHDDVSLlanagalpMDIANGMi-ENVIGTFELPYAVASNFQINGRDVLVPLVV 81  Pseudomonas mevalonii
1QAX_B      8 PAFRNLsp--aARLDHIGqll---glsHDDVSLlanagalpMDIANGMi-ENVIGTFELPYAVASNFQINGRDVLVPLVV 81  Pseudomonas mevalonii
1QAY_A      8 PAFRNLsp--aARLDHIGqll---glsHDDVSLlanagalpMDIANGMi-ENVIGTFELPYAVASNFQINGRDVLVPLVV 81  Pseudomonas mevalonii
1QAY_B      8 PAFRNLsp--aARLDHIGqll---glsHDDVSLlanagalpMDIANGMi-ENVIGTFELPYAVASNFQINGRDVLVPLVV 81  Pseudomonas mevalonii
1DQA_A     58 YKLETLietheRGVSIRRqllskklsePSSLQYlpyr-dynYSLVMGAccENVIGYMPIPVGVAGPLCLDEKEFQVPMAT 136 human
EET00684  101 ALQITLaq-akVRSDHEV---------SKYTQR--------IQALDAMs-ENVIGVTMLPLSVVPDVPIDGTMYTVPIST 161 Giardia intestinal...
Feature 1      #                                                                              
1QAX_A     82 EEPSIVAAASYMAKLARAng-gFTTSSSAPLMHAQVQIVGiq--dPLNARLSLLRr--KDEIIELANRKdqlln-slggg 155 Pseudomonas mevalonii
1QAX_B     82 EEPSIVAAASYMAKLARAng-gFTTSSSAPLMHAQVQIVGiq--dPLNARLSLLRr--KDEIIELANRKdqlln-slggg 155 Pseudomonas mevalonii
1QAY_A     82 EEPSIVAAASYMAKLARAng-gFTTSSSAPLMHAQVQIVGiq--dPLNARLSLLRr--KDEIIELANRKdqlln-slggg 155 Pseudomonas mevalonii
1QAY_B     82 EEPSIVAAASYMAKLARAng-gFTTSSSAPLMHAQVQIVGiq--dPLNARLSLLRr--KDEIIELANRKdqlln-slggg 155 Pseudomonas mevalonii
1DQA_A    137 TEGCLVASTNRGCRAIGLgg-gASSRVLADGMTRGPVVRLpracdSAEVKAWLETsegFAVIKEAFDSTsrf------ar 209 human
EET00684  162 EEPSVVAALAHAAKIFRAgsgvRTLAPVSPFILGQLIFLLt----DEQWKHPMNLs-gLEQLLSDSLTRhlpnsrnygkg 236 Giardia intestinal...
Feature 1                                                                                     
1QAX_A    156 CRDIEVHTfadtprgPMLVAHLIVDVRDAMGANTVNTMAEAVAPLMEAITGg---QVRLRILsnl------------adl 220 Pseudomonas mevalonii
1QAX_B    156 CRDIEVHTfadtprgPMLVAHLIVDVRDAMGANTVNTMAEAVAPLMEAITGg---QVRLRILsnl------------adl 220 Pseudomonas mevalonii
1QAY_A    156 CRDIEVHTfadtprgPMLVAHLIVDVRDAMGANTVNTMAEAVAPLMEAITGg---QVRLRILsnl------------adl 220 Pseudomonas mevalonii
1QAY_B    156 CRDIEVHTfadtprgPMLVAHLIVDVRDAMGANTVNTMAEAVAPLMEAITGg---QVRLRILsnl------------adl 220 Pseudomonas mevalonii
1DQA_A    210 LQKLHTSIa-----gRNLYIRFQSRSGDAMGMNMISKGTEKALSKLHEYFPe--mQILAVSGnyctdkkpaainwiegrg 282 human
EET00684  237 IVSCYLRKl-----nEYIVLEIGLDPGEAMGANMINGFLEAIGPDVENYLSrfgsVERLTSIlsns-----------gvg 300 Giardia intestinal...
Feature 1                                                                          #          
1QAX_A    221 RLARAQVRITPQQLETaefsgeaVIEGILDAYAf----AAVDPyraa---thnkGIMNGIDPLIVATGNDWRAvEAGAHa 293 Pseudomonas mevalonii
1QAX_B    221 RLARAQVRITPQQLETaefsgeaVIEGILDAYAf----AAVDPyraa---thnkGIMNGIDPLIVATGNDWRAvEAGAHa 293 Pseudomonas mevalonii
1QAY_A    221 RLARAQVRITPQQLETaefsgeaVIEGILDAYAf----AAVDPyraa---thnkGIMNGIDPLIVATGNDWRAvEAGAHa 293 Pseudomonas mevalonii
1QAY_B    221 RLARAQVRITPQQLETaefsgeaVIEGILDAYAf----AAVDPyraa---thnkGIMNGIDPLIVATGNDWRAvEAGAHa 293 Pseudomonas mevalonii
1DQA_A    283 KSVVCEAVIPAKVVREvl---ktTTEAMIEVNIn---kNLVGSamagsiggynaHAANIVTAIYIACGQDAAQ-NVGSSn 355 human
EET00684  301 REYEASAKITVEEYAAmknitlkKAEDVLRRIEalsrlAEIDSnrri---thnkGVLNGVIGVALACGQDTRAvDVANCq 377 Giardia intestinal...
Feature 1                                                                                     
1QAX_A    294 yacrsghygsLTTWEKDnn--gHLVGTLEM-PMPVGLVGGATKt----HPLAQLSLRILGVkt---------aQALAEIA 357 Pseudomonas mevalonii
1QAX_B    294 yacrsghygsLTTWEKDnn--gHLVGTLEM-PMPVGLVGGATKt----HPLAQLSLRILGVkt---------aQALAEIA 357 Pseudomonas mevalonii
1QAY_A    294 yacrsghygsLTTWEKDnn--gHLVGTLEM-PMPVGLVGGATKt----HPLAQLSLRILGVkt---------aQALAEIA 357 Pseudomonas mevalonii
1QAY_B    294 yacrsghygsLTTWEKDnn--gHLVGTLEM-PMPVGLVGGATKt----HPLAQLSLRILGVkt---------aQALAEIA 357 Pseudomonas mevalonii
1DQA_A    356 ---------cITLMEASgptneDLYISCTMpSIEIGTVGGGTN-----LLPQQACLQMLGVqgackdnpgenaRQLARIV 421 human
EET00684  378 lghm--kplvTHRFNSNe---mFLRITARI-SAPVGVIGGSSKlfwglSILGQAHDNRISS------------SSLGRIL 439 Giardia intestinal...
Feature 1                            #     
1QAX_A    358 VAVGLAQNLGAMRALATEGIQRGHMALHA 386 Pseudomonas mevalonii
1QAX_B    358 VAVGLAQNLGAMRALATEGIQRGHMALHA 386 Pseudomonas mevalonii
1QAY_A    358 VAVGLAQNLGAMRALATEGIQRGHMALHA 386 Pseudomonas mevalonii
1QAY_B    358 VAVGLAQNLGAMRALATEGIQRGHMALHA 386 Pseudomonas mevalonii
1DQA_A    422 CGTVMAGELSLMAALAAGHLVKSHMIHNR 450 human
EET00684  440 AAVGLLQNFAALQALVTNGIQYGHMKLHS 468 Giardia intestinalis ATCC 50581

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap