6PFK,1PFK,1PFK,1KZH


Conserved Protein Domain Family
PFK
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cd00363: PFK 
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Phosphofructokinase, a key regulatory enzyme in glycolysis, catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-biphosphate. The members belong to PFK family that includes ATP- and pyrophosphate (PPi)- dependent phosphofructokinases. Some members evolved by gene duplication and thus have a large C-terminal/N-terminal extension comprising a second PFK domain. Generally, ATP-PFKs are allosteric homotetramers, and PPi-PFKs are dimeric and nonallosteric except for plant PPi-PFKs which are allosteric heterotetramers.
Links
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Statistics
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PSSM-Id: 238216
Aligned: 16 rows
Threshold Bit Score: 421.707
Created: 6-Mar-2002
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 17 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1PFK_A; closed active subunit conformation; Mg2+ bridges the two substrates, Fru-1,6-P and ADP
  • Structure:1PFK_B; open subunit conformation
  • Citation:PMID 2975709
Scroll to Sequence Alignment Display
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1              #                               #                               #      
6PFK_D      2 KRIGVLTSGGDSPGMNAAIRSVVRKAIYHG--VEVYGVYHGYAGLIAGNIKKLEVGDVGDIIHRGGT-ILYTARCpefkt 78  Geobacillus stearo...
1PFK_A      3 KKIGVLTSGGDAPGMNAAIRGVVRSALTEG--LEVMGIYDGYLGLYEDRMVQLDRYSVSDMINRGGT-FLGSARFpefrd 79  Escherichia coli
1PFK_B      3 KKIGVLTSGGDAPGMNAAIRGVVRSALTEG--LEVMGIYDGYLGLYEDRMVQLDRYSVSDMINRGGT-FLGSARFpefrd 79  Escherichia coli
1KZH_A     73 LNIGIILSGGPAPGGHNVISGVFDAIKKFNpnSKLFGFKGGPLGLLENDKIELTESLINSYRNTGGFdIVSSGRTki-et 151 Borrelia burgdorferi
NP_218981  81 LRVAIVLSGGQAPGGHNVIAGLFDGLKRWHadSVLIGFLGGPAGVLSGDHIEICADRVDAYRNTGGFdLIGSGRTki-es 159 Treponema pallidum
547755      2 KRIGILTSGGDAPGMNAAVRAVTRVAIANG--LEVFGIRYGFAGLVAGDIFPLESEDVAHLINVSGT-FLYSARYpefae 78  Lactobacillus delb...
585336      2 KRIAVLTSGGDAPGMNAAIRAVVRKAISEG--IEVYGINHGYAGMVAGDIFPLTSASVGDKIGRGGT-FLYSARYpefaq 78  Lactococcus lactis...
1170602     3 KKIAVLTSGGDAPGMNAAIRGVVRSALAEG--LEVFGIYDGYQGLYNNKIKQLNRYSVSDVINRGGT-FLGSARFpefkd 79  Haemophilus influe...
125120      2 KRIGVFTSGGDAPGMNAAIRAVVRQAHALG--VEVIGIRRGYAGMIQGEMVPLGVRDVANIIQRGGT-ILLTARSqeflt 78  Thermus thermophilus
1346354     3 KKIGILTSGGDSQGMNAAIAGVIKTAHAKG--LETYIIRDGYLGLINNWIEVVDNNFADSIMLLGGT-VIGSARLpefkd 79  Spiroplasma citri

Feature 1                             ### ##                      # # #                       
6PFK_D     79 EEGQKKGIEQLKKHGIEGLVVIGGDGSYQGAKKLTEH------GFPCVGVPGTIDNDIp--gTDFTIGFDTALNTVIDAI 150 Geobacillus stearo...
1PFK_A     80 ENIRAVAIENLKKRGIDALVVIGGDGSYMGAMRLTEM------GFPCIGLPGTIDNDIk--gTDYTIGFFTALSTVVEAI 151 Escherichia coli
1PFK_B     80 ENIRAVAIENLKKRGIDALVVIGGDGSYMGAMRLTEM------GFPCIGLPGTIDNDIk--gTDYTIGFFTALSTVVEAI 151 Escherichia coli
1KZH_A    152 EEHYNKALFVAKENNLNAIIIIGGDDSNTNAAILAEYfkkngeNIQVIGVPKTIDADLrndhIEISFGFDSATKIYSELI 231 Borrelia burgdorferi
NP_218981 160 ESQFAAAAQTVTRMALDALVVVGGDDSNTNAALLAEHfvnsgiSTKVIGVPKTIDGDLkneaIETSFGFDTATKTYSELI 239 Treponema pallidum
547755     79 EEGQLAGIEQLKKHGIDAVVVIGGDGSYHGALQLTRH------GFNSIGLPGTIDNDIp--yTDATIGYDTACMTAMDAI 150 Lactobacillus delb...
585336     79 VEGQLAGIEQLKKFGIEGVVVIGGDGSYHGAMRLTEH------GFPAVGLPGTIDNDIv--gTDFTIGFDTAVSTVVDAL 150 Lactococcus lactis...
1170602    80 PNIRAKCAEILRSHGIDALVVIGGDGSYMGAKLLTEEh-----SFPCVGLPGTIDNDVa--gTDYTIGYQTALQTAVDAI 152 Haemophilus influe...
125120     79 EEGRAKAYAKLQAAGIEGLVAIGGDGTFRGALFLVEEh-----GMPVVGVPGTIDNDLy--gTDYTIGFDTAVNTALEAI 151 Thermus thermophilus
1346354    80 PEVQKKAVDILKKQEIAALVVIGGDGSYQGAQRLTEL------GINCIALPGTIDNDIt--sSDYTIGFDTAINIVVEAI 151 Spiroplasma citri

Feature 1                        ###                                                          
6PFK_D    151 DKIRDTATS-HERTYVIEVMGRHAGDIALWSGLAGGAETILIPEAdy--------dMNDVIARLKRGHERGKKHSIIIVA 221 Geobacillus stearo...
1PFK_A    152 DRLRDTSSS-HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVef--------sREDLVNEIKAGIAKGKKHAIVAIT 222 Escherichia coli
1PFK_B    152 DRLRDTSSS-HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVef--------sREDLVNEIKAGIAKGKKHAIVAIT 222 Escherichia coli
1KZH_A    232 GNLCRDAMStKKYWHFVKLMGRSASHVALECALKTHPNICIVSEEvlakkktlseiIDEMVSVILKRSLNGDNFGVVIVP 311 Borrelia burgdorferi
NP_218981 240 GNIARDACSaRKYWHFIKLMGRSASHIALECALKTQPNVCLISEEvaaqsltlaqiVQSLCDTIATRAQHGEHFGIVLVP 319 Treponema pallidum
547755    151 DKIRDTASS-HHRVFIVNVMGRNCGDIAMRVGVACGADAIVIPERpy--------dVEEIANRLKQAQESGKDHGLVVVA 221 Lactobacillus delb...
585336    151 DKIRDTSSS-HNRTFVVEVMGRNAGDIALNAGIAAGADDICIPEKef--------kFENVVNNINKGYEKGKNHHIIVLA 221 Lactococcus lactis...
1170602   153 DRLRDTSSS-HQRISIVEIMGRHCSDLTISAGIAGGCEYIVASEIef--------nREELIQQIERSIIRGKRHAIIAIT 223 Haemophilus influe...
125120    152 DRIRDTAAS-HERVFFIEVMGRHAGFIALDVGLAGGAEVIAVPEEpv--------dPKAVAEVLEASQRRGKKSSIVVVA 222 Thermus thermophilus
1346354   152 DRLRDTMQS-HNRCSIVEVMGHACGDIALYAGIAGGADIISINEAal--------sETEIADRVAMLHQAQKRSVIVVVS 222 Spiroplasma citri

Feature 1     #                                                                               
6PFK_D    222 EGVg---------------------------------------------------------------------------- 225 Geobacillus stearo...
1PFK_A    223 EHMc---------------------------------------------------------------------------- 226 Escherichia coli
1PFK_B    223 EHMc---------------------------------------------------------------------------- 226 Escherichia coli
1KZH_A    312 EGLiefipevkslmlelcdifdknegefkglniekmkeifvaklsdymkgvylslplfiqfeliksilerdphgnfnvsr 391 Borrelia burgdorferi
NP_218981 320 EGLiefipemkalitelnevmarraqefealdtpdaqrvwie--qalsasaravfnalpaeistqlladrdphgnvqvsr 397 Treponema pallidum
547755    222 EGVm---------------------------------------------------------------------------- 225 Lactobacillus delb...
585336    222 EGVm---------------------------------------------------------------------------- 225 Lactococcus lactis...
1170602   224 ELLt---------------------------------------------------------------------------- 227 Haemophilus influe...
125120    223 EGAyp--------------------------------------------------------------------------- 227 Thermus thermophilus
1346354   223 EMIyp--------------------------------------------------------------------------- 227 Spiroplasma citri

Feature 1                                         #  #                                        
6PFK_D    226 --sGVDFGRQIQEAt-----------gFETRVTVLGHVQRGGSPTAFDRVLASRLGARAVELLLEGKGGRCVGIQNn--- 289 Geobacillus stearo...
1PFK_A    227 --dVDELAHFIEKEt-----------gRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLLLAGYGGRCVGIQNe--- 290 Escherichia coli
1PFK_B    227 --dVDELAHFIEKEt-----------gRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLLLAGYGGRCVGIQNe--- 290 Escherichia coli
1KZH_A    392 vptEKLFIEMIQSRlndmkkrgeykgsFTPVDHFFGYEGRSAFPSNFDSDYCYSLGYNAVVLILNGLTGYMSCIKNlnlk 471 Borrelia burgdorferi
NP_218981 398 idtERLLILQVTERlaqmkqegtytgvFSSIAHFFGYEGRCAFPSNFDADYCYTLGLTACLLAVHRFTGYVASVRNltss 477 Treponema pallidum
547755    226 --tADQFMAELKKYg-----------dFDVRANVLGHMQRGGTPTVSDRVLASKLGSEAVHLLLEGKGGLAVGIENg--- 289 Lactobacillus delb...
585336    226 --tGEEFATKLKEAgy----------kGDLRVSVLGHIQRGGSPTARDRVLASRMGARAVELLRDGIGGVAVGIRNe--- 290 Lactococcus lactis...
1170602   228 --dVHSLAKEIEARv-----------gHETRATVLGHIQRGGSPCAFDRILASRMGAYAVDLLLQGKGGYCVGIQNe--- 291 Haemophilus influe...
125120    228 -ggAAGLLAAIREHl-----------qVEARVTVLGHIQRGGSPTAKDRILASRLGAPAVEALVGGASGVMVGEVEg--- 292 Thermus thermophilus
1346354   228 --dVHKLAKLVESKs-----------gYITRATVLGHTQRGGNPTAMDRYRAFQMAQFAVEQIIAGVGGLAIGNQGd--- 291 Spiroplasma citri

Feature 1                                                  
6PFK_D    290 ----QLVDHDIAEALAnkht--------------idqRMYALSKE 316 Geobacillus stearothermophilus
1PFK_A    291 ----QLVHHDIIDAIEnmkr-------------pfkgDWLDCAKK 318 Escherichia coli
1PFK_B    291 ----QLVHHDIIDAIEnmkr-------------pfkgDWLDCAKK 318 Escherichia coli
1KZH_A    472 ptdwIAGGVPLTMLMNmeerygekkpvikkalvdlegRPFKEFVK 516 Borrelia burgdorferi
NP_218981 478 vaewAVGGVPLTMLMNmerrhgsqkpvikkalvdlegMPFRVFSR 522 Treponema pallidum
547755    290 ----KVTSHDILDLFDeshr--------------gdyDLLKLNAD 316 Lactobacillus delbrueckii subsp. bulgaricus
585336    291 ----ELVESPILGTAEegalfsl-------tteggikVNNPHKAG 324 Lactococcus lactis subsp. lactis
1170602   292 ----QLVHHDIIDAINnmqr-------------vfkaDWLKVAKR 319 Haemophilus influenzae
125120    293 ----EVDLTPLKEAVErrkd--------------inrALLRLSQV 319 Thermus thermophilus
1346354   292 ----QIIARPIMEALSiprs--------------srkEIWAKFDQ 318 Spiroplasma citri

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