1ECC,1AO0,2BPJ,1EA0,1EA0,1OFE,1OFE,1TE5,1MBZ,1Q15,1CT9


Conserved Protein Domain Family
Gn_AT_II
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cd00352: Gn_AT_II 
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Glutamine amidotransferases class-II (GATase). The glutaminase domain catalyzes an amide nitrogen transfer from glutamine to the appropriate substrate. In this process, glutamine is hydrolyzed to glutamic acid and ammonia. This domain is related to members of the Ntn (N-terminal nucleophile) hydrolase superfamily and is found at the N-terminus of enzymes such as glucosamine-fructose 6-phosphate synthase (GLMS or GFAT), glutamine phosphoribosylpyrophosphate (Prpp) amidotransferase (GPATase), asparagine synthetase B (AsnB), beta lactam synthetase (beta-LS) and glutamate synthase (GltS). GLMS catalyzes the formation of glucosamine 6-phosphate from fructose 6-phosphate and glutamine in amino sugar synthesis. GPATase catalyzes the first step in purine biosynthesis, an amide transfer from glutamine to PRPP, resulting in phosphoribosylamine, pyrophosphate and glutamate. Asparagine synthetase B synthesizes asparagine from aspartate and glutamine. Beta-LS catalyzes the formation of the beta-lactam ring in the beta-lactamase inhibitor clavulanic acid. GltS synthesizes L-glutamate from 2-oxoglutarate and L-glutamine. These enzymes are generally dimers, but GPATase also exists as a homotetramer.
Links
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Statistics
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PSSM-Id: 238212
Aligned: 637 rows
Threshold Bit Score: 59.3845
Created: 6-Mar-2002
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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active site
Conserved site includes 6 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1CT9_A, E.coli Asn_B bound to glutamine using 3.5A
Scroll to Sequence Alignment Display
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1     #                                                                               
1ECC_B      1 CGIVGIAgvmpvnqsiyd-------------------------------------------------------------- 18   Escherichia coli
AAL39601    2 CGIFCSVvnnvplnsfnis------------------------------------------------------------- 20   fruit fly
XP_380780   2 CGIHASIntspdqalsse-------------------------------------------------------------- 19   Gibberella zeae PH-1
XP_962154   2 CGIHAVLtppgvthivss-------------------------------------------------------------- 19   Neurospora crassa...
XP_759646   2 CGIGILIapvsaaqqgqhsthsddap------------------------------------------------------ 27   Ustilago maydis 521
NP_013613   2 CGILLHYcpnnnylndeliefpegtefgd--------------------------------------------------- 30   baker's yeast
CAG60032    2 CGILLCYgpsfkaendrllefyedevp----------------------------------------------------- 28   Candida glabrata ...
AAS51569    2 CGILVHYseveadsdrfvefperceppevq-------------------------------------------------- 31   Ashbya gossypii A...
XP_453519   2 CGILAYFgksfgstltdefqelyekdltgp-------------------------------------------------- 31   Kluyveromyces lac...
XP_714517   2 CGILLKVsnkpldipscipqplrqtdndqktnsnqhqqqhqqwetldkaiiesflnkgselkpltnqqiyklnnlshlre 81   Candida albicans ...

Feature 1                                                                                     
1ECC_B     19 ----------------------------------------------------------------altvlqhrgqDAAGII 34   Escherichia coli
AAL39601   21 --------------------------------------------------------------salkvilknrgpDVQDEV 38   fruit fly
XP_380780  20 ----------------------------------------------------------------lerclcnrgpDHTGTV 35   Gibberella zeae PH-1
XP_962154  20 ---------------------------------------------------------------dltrclcnrgpDYLGQV 36   Neurospora crassa...
XP_759646  28 -------------------------------------------------------splqrcwpsvlqnianrgpDCLNTV 52   Ustilago maydis 521
NP_013613  31 ----------------------------------------------------ttctnessifnkiipyiaargpNYSSLR 58   baker's yeast
CAG60032   29 ------------------------------------------------------rlgyddniesivklvrcrgpNYCSMR 54   Candida glabrata ...
AAS51569   32 ----------------------------------------------------khcdgasqeflrlvpyimargpDYASYR 59   Ashbya gossypii A...
XP_453519  32 ----------------------------------------------------iqscnsntlfnelipyivrrgpNYASLR 59   Kluyveromyces lac...
XP_714517  82 lnnqlsklknnvkrlssdleyqrqeqikqlqteidliskdyddddndgleidrqkgegeddldqliynisnrgpDYLNFS 161  Candida albicans ...

Feature 1                                           #                              ###        
1ECC_B     35 tidanncfrlrkanglvsdvfearhmqrlqgnmGIGHVRyptagsssaseaqPFYvnsp---ygiTLAHNGNLTNAHELr 111  Escherichia coli
AAL39601   39 vidyc-----------------------fgkilFAGFVLwqqge---svqkqPVVed------dfIFLFNGDIYNTSKPe 86   fruit fly
XP_380780  36 ktqldns-------------------hgelfitFTSTVLslrgd---hvarqPFVdpa----tgsVLCWNGEAWALRGEp 89   Gibberella zeae PH-1
XP_962154  37 errvtsstnd--------------dstdcwtlkLTSTVLalrgd---hvakqPLSdldt--dkgsVLCWNGEAWRINGEp 97   Neurospora crassa...
XP_759646  53 qhhfrindss--------------saaaswtlsFTSSVLslrgd---gvtqqPLCsid----grlLLAWNGQIFDWDAPe 111  Ustilago maydis 521
NP_013613  59 av-----------------------------kaYRISWFssvl-----slrqPFTkqsinvddryFLQFNGELYNKEISq 104  baker's yeast
CAG60032   55 qi-----------------------------kdVNSIWIssvl-----slrqPFTkqsivignkyVLQFNGELYNDEIIe 100  Candida glabrata ...
AAS51569   60 le-----------------------------agMGLAWFssvl-----slrqPLTpqsmavgdryILQFNGELFNEDIQh 105  Ashbya gossypii A...
XP_453519  60 slse-------------------------inamLFSSVLslrtp----ftkqSVMvqd-----ryLLQYNGELYNDDTVe 105  Kluyveromyces lac...
XP_714517 162 qfnttt----------------------nngdeFHFQTFsail-----slrqPFTrqpif-kdqfVLQFNGELYNQECLq 213  Candida albicans ...

Feature 1                           #                                                         
1ECC_B    112 kklfeek-------rrhinttSDSEILLNIFASEldnfrhypl----eadnifaaiAATNrlirGAYACVAMIig-hGMV 179  Escherichia coli
AAL39601   87 y-------------------mSDTTWIAERLAECrcke---------------qniLKILkrleGPHCLIIYDkreqILY 132  fruit fly
XP_380780  90 v------------------qgNDGEAILALLAEVskds---------------gdvMDVLrdidGPFAFIYLDkpakRLY 136  Gibberella zeae PH-1
XP_962154  98 v------------------sgNDGEEIWRMLRGVeanasvtttipilmeeekeehiLDVFraieGPFAFVYWHeasrKVF 159  Neurospora crassa...
XP_759646 112 desdgdrnratssrvrldsgeNDAIVLLDSIQRLletqhrdgs----qvcsvqhalNTALsqveGPYAFVLLDclesKLY 187  Ustilago maydis 521
NP_013613 105 g-------------------dNDSLYIASMLQNLkeg----------------mgvIDVIksleGEYAYTIYDvnssKLY 149  baker's yeast
CAG60032  101 s-------------------gNDTQFLSGLLKKCise----------------ddiFNILhgldGEFAFTIQDlennLIY 145  Candida glabrata ...
AAS51569  106 ---------------------NDTQYIADLLASWp--------------------vTAVIrqmyGEFAYTIFDrqdgLLY 144  Ashbya gossypii A...
XP_453519 106 nc-----------------ehSDTEYISSLLEKHs--------------------vEEVVrkcyGEFAYAITDlqerKVY 148  Kluyveromyces lac...
XP_714517 214 --------------------iNDTQFIIDKLHEHlqvndn-----------rnnaiLSTLkslnGEFAIILIDllenKIY 262  Candida albicans ...

Feature 1                                                                                     
1ECC_B    180 AFRdpngirPLVLGkrdidenrtEYMVASESVALdtlg-------------------------------fdflRDVAPGE 228  Escherichia coli
AAL39601  133 FSRdalgrnSLLIEric---ngfQLLSTSHFEDKki------------------------------------sLELPPLG 173  fruit fly
XP_380780 137 YGRdrlgrrSLLVKde------lPFSLSSIAETPvd-----------------------------------gwTEVEADG 175  Gibberella zeae PH-1
XP_962154 160 FGRdrlgrrSLMMKrde---rtgEVVLSSVAEGLsggda-----------------------------nangwKEVEADG 207  Neurospora crassa...
XP_759646 188 FGRdplgrrSLLLHrsp---qysTLSIVSVAAAPlms----------------------------------aaEEVGASL 230  Ustilago maydis 521
NP_013613 150 FGRdpigrrSLSYSvtp----dnELYVASVTGSAgs------------------------------------fQDCIGGV 189  baker's yeast
CAG60032  146 FGRdtigkrSLSYSlny---nisELIVTSVSGSDngs---------------------------------rifNDCVAGV 189  Candida glabrata ...
AAS51569  145 FGRdnvgkrSLCYRld-----grELHVASVSGKSqg------------------------------------fLNCDAGV 183  Ashbya gossypii A...
XP_453519 149 FGRdsigkrSLSYIll-----ddELYITSVSGRCdg------------------------------------fQNCLAGV 187  Kluyveromyces lac...
XP_714517 263 FGRdsigkrSLCYKlsgg-vknkQLIISSVSNKGfidceniiyeynlgyntlakhklhelpshaniesgaikdEEQLVKN 341  Candida albicans ...

Feature 1       
1ECC_B    229 AI 230  Escherichia coli
AAL39601  174 LF 175  fruit fly
XP_380780 176 CY 177  Gibberella zeae PH-1
XP_962154 208 IY 209  Neurospora crassa N150
XP_759646 231 TE 232  Ustilago maydis 521
NP_013613 190 IY 191  baker's yeast
CAG60032  190 IY 191  Candida glabrata CBS 138
AAS51569  184 IY 185  Ashbya gossypii ATCC 10895
XP_453519 188 IY 189  Kluyveromyces lactis NRRL Y-1140
XP_714517 342 LY 343  Candida albicans SC5314

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