1DQV,1W16,2R83


Conserved Protein Domain Family
C2B_Synaptotagmin

?
cd00276: C2B_Synaptotagmin 
Click on image for an interactive view with Cn3D
C2 domain second repeat present in Synaptotagmin
Synaptotagmin is a membrane-trafficking protein characterized by a N-terminal transmembrane region, a linker, and 2 C-terminal C2 domains. There are several classes of Synaptotagmins. Previously all synaptotagmins were thought to be calcium sensors in the regulation of neurotransmitter release and hormone secretion, but it has been shown that not all of them bind calcium. Of the 17 identified synaptotagmins only 8 bind calcium (1-3, 5-7, 9, 10). The function of the two C2 domains that bind calcium are: regulating the fusion step of synaptic vesicle exocytosis (C2A) and binding to phosphatidyl-inositol-3,4,5-triphosphate (PIP3) in the absence of calcium ions and to phosphatidylinositol bisphosphate (PIP2) in their presence (C2B). C2B also regulates also the recycling step of synaptic vesicles. C2 domains fold into an 8-standed beta-sandwich that can adopt 2 structural arrangements: Type I and Type II, distinguished by a circular permutation involving their N- and C-terminal beta strands. Many C2 domains are Ca2+-dependent membrane-targeting modules that bind a wide variety of substances including bind phospholipids, inositol polyphosphates, and intracellular proteins. Most C2 domain proteins are either signal transduction enzymes that contain a single C2 domain, such as protein kinase C, or membrane trafficking proteins which contain at least two C2 domains, such as synaptotagmin 1. However, there are a few exceptions to this including RIM isoforms and some splice variants of piccolo/aczonin and intersectin which only have a single C2 domain. C2 domains with a calcium binding region have negatively charged residues, primarily aspartates, that serve as ligands for calcium ions. This cd contains the second C2 repeat, C2B, and has a type-I topology.
Statistics
?
PSSM-Id: 175975
View PSSM: cd00276
Aligned: 57 rows
Threshold Bit Score: 103.432
Threshold Setting Gi: 221132417
Created: 1-Nov-2000
Updated: 2-Oct-2020
Structure
?
Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
1DQV_A       139 GELNFSLCYLPtaGLLTVTIIKASNLKamd-------ltgFSDPYVKASLISeg---rRLKKRKTSIKKNt-LNPTYNEA 207 black rat
XP_787138    165 GILNVSICYRSriRKLCITVIRAQNLSktgf------cktSPDCRIKVAVTQvkdnalSTVVKRTRVRRHt-RQPVFKES 237 purple urchin
XP_001637842   1 GDICLVLQYYReeGQLTVTVKQIRGLKpfi-------grkTTSAYVKVHVVQgs---kELDERRTKVKRKn-FEPEYNET 69  starlet sea ane...
XP_002587241  85 GEILFTLNYLPtaEKLTVVVIKVKDMTnqsi-----ifyfLAAMFVKVCLVQh----hKQVKKKTRLHKIa-PEIVLNDS 154 Florida lancelet
XP_002121935 438 PHLQLSLCYHAmlGFVTVEVIAARNIPkgs-------lgrSQDCYVVLAEEPps---gKSAKKQTKISHRn-QDPVFQES 506 Ciona intestinalis
XP_786691     60 GRLRLVLCYHRekRRLLVTVVRATNLPsr---------viPADSYVVVELRGsd--neVLAQVRTKERHFs-NNPVYKEV 127 purple urchin
XP_421322    352 TELQIGTCFQAinRRIQLQILEAQNLPiss-------tplSLSFFVKVGMFSte---gLIYKKKTRLVKStnGQVKWREM 421 chicken
BAC86538     289 AELELGTCFQAvnSRIQLQILEARYLPsss-------tplTLSFFVKVGMFSsg---eLIYKKKTRLLKAsnGRVKWGET 358 human
XP_001633552 155 GRVYVSLSYDAvtQRLKVTLLKAKNLSckrtegkdkcginCSDSFVKFTLLKge---kEVKSHESAIKKGy-SEVLYYEN 230 starlet sea ane...
XP_002155428 250 AEVYIHTTYLAsaQRFSVVLSQAKNLNid---------pfNKEIQGRVTLIHlh---sERKTYSTDLLQAk-KHVNFNEE 316 green hydra
1DQV_A       208 LVFDVApesv------------envGLSIAVVDYDci------gHNEVIGVCRVgpea--------adphGREHWAEMla 261 black rat
XP_787138    238 MSFEINdlnnl----------rdgmTVVCSLSGKSlt------gKPQTLGQVRFglga--------sretEKLQWANVlr 293 purple urchin
XP_001637842  70 FKFNIPqnii------------eevNLIIRVKDSPyv------gSKTLLGEVCVggda---------vgtYLHHWRLIle 122 starlet sea ane...
XP_002587241 155 ISFDLPhehld----------dvraILFLCIPQYTsrs---gsiRYDVLGQVMVgsgc---------rgpGQEHWIQMtr 212 Florida lancelet
XP_002121935 507 FIFSMPnd---------------qlVILATLFVKDkvkgnwvscGQVKLGQAVStw-------------sGQLQWAQAld 558 Ciona intestinalis
XP_786691    128 FSFDLPsrpiedwdftniqhpldnvSLTITVHRRKlf------fGDDTIGCVTLrsev--------rtngYSHLWKCKrs 193 purple urchin
XP_421322    422 MIFPVSqae-------------qgiGFLIKLYSRSsv------rRKHFLGQVWIssds--------nnseAVEQWEDTia 474 chicken
BAC86538     359 MIFPLIqse-------------keiVFLIKLYSRSsv------rRKHFVGQIWIseds--------nnieAVNQWKETvi 411 human
XP_001633552 231 HAFDIEitgl------------qtlSLIITAVHCCrph---lihHNHEIGRVVIpadlnnesvvesqlmeALTHPGQV-- 293 starlet sea ane...
XP_002155428 317 FVFQLTdldiq---------ttegiDFKIEILCRIkkt----fsKPIVIGNFFIgtsis-------cgrfGNEHWKAMms 376 green hydra
1DQV_A       262 nprkpVEHWHQL 273 black rat
XP_787138    294 npglsITQWHTL 305 purple urchin
XP_001637842 123 k-gheIEMWHAL 133 starlet sea anemone
XP_002587241 213 sprrpVARWHLV 224 Florida lancelet
XP_002121935 559 regqpVTFWHLL 570 Ciona intestinalis
XP_786691    194 p-yqpITEWHDL 204 purple urchin
XP_421322    475 npekvVIKWYSL 486 chicken
BAC86538     412 npekvVIRWHKL 423 human
XP_001633552 294 -----IYEWHTI 300 starlet sea anemone
XP_002155428 377 s-nstISRWHQL 387 green hydra
| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap