1DJX,2FJU,1QAS,1DJG


Conserved Protein Domain Family
C2_PLC_like

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cd00275: C2_PLC_like 
Click on image for an interactive view with Cn3D
C2 domain present in Phosphoinositide-specific phospholipases C (PLC)
PLCs are involved in the hydrolysis of phosphatidylinositol-4,5-bisphosphate (PIP2) to d-myo-inositol-1,4,5-trisphosphate (1,4,5-IP3) and sn-1,2-diacylglycerol (DAG). 1,4,5-IP3 and DAG are second messengers in eukaryotic signal transduction cascades. PLC is composed of a N-terminal PH domain followed by a series of EF hands, a catalytic TIM barrel and a C-terminal C2 domain. C2 domains fold into an 8-standed beta-sandwich that can adopt 2 structural arrangements: Type I and Type II, distinguished by a circular permutation involving their N- and C-terminal beta strands. Many C2 domains are Ca2+-dependent membrane-targeting modules that bind a wide variety of substances including bind phospholipids, inositol polyphosphates, and intracellular proteins. Most C2 domain proteins are either signal transduction enzymes that contain a single C2 domain, such as protein kinase C, or membrane trafficking proteins which contain at least two C2 domains, such as synaptotagmin 1. However, there are a few exceptions to this including RIM isoforms and some splice variants of piccolo/aczonin and intersectin which only have a single C2 domain. C2 domains with a calcium binding region have negatively charged residues, primarily aspartates, that serve as ligands for calcium ions. Members here have a type-II topology.
Statistics
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PSSM-Id: 175974
View PSSM: cd00275
Aligned: 163 rows
Threshold Bit Score: 71.0332
Threshold Setting Gi: 17566272
Created: 1-Nov-2000
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Ca2+ binding
Conserved site includes 6 residues -Click on image for an interactive view with Cn3D
Feature 1:Ca2+ binding pocket [ion binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                         #                                        #     
1DJX_B       496 PERLRVRIISGQQLPKvnk--------nknSIVDPKVIVEIHGVGr-----------------dTGSRQTAVITNNGfNP 550 Norway rat
EEU42658     473 EVKLSITFYSGQNIPLpedc-------tslQKFKPYVSVELHVEGpsdkhgd-----esesyerNEKYTDFTISHKGcDI 540 Nectria haemato...
EEU46167     481 TLHLTITVLAAQNIPLppgd-------tsaRGFEPYVKVELHVEGpdefhggp---ipndgherEGEYKARTRTHRGqAP 550 Nectria haemato...
EEU47360     578 TMNLRITVFAGQRIPVqagdaa--dhnrsaSTIRPLIKVELHVDKpddver--------dgqmqEYNYKGRTRSSKTdHP 647 Nectria haemato...
XP_001222548 539 TLDLQITILAGQFLPLpgdrkssgfgiksdRSFRPKVKVELHVEKaek-------------pqrSVDCARETVTARTeHP 605 Chaetomium glob...
XP_001545984 344 TLDLKITIFAGQQIPYpeg--------eteKGFHPYVKCELHVESqeelngqp---igndvkakEGEHKLKTAYKSGsKP 412 Botryotinia fuc...
EEH43765     525 TLNLSIEIYGGQNIPLpegd-------ehaRSFRPYVTCQLHVERprdsvhas---endkngneAAKYKQKTKSASGsDP 594 Paracoccidioide...
AAZ23804     552 TMDLTINVLAAQSLPLpprd-------nnvDSFRPYLKVELHVEEpgerhgtdelpqdgkekggEYKAKTKSVKGGC-DP 623 Neurospora crassa
EDP52946     504 QLDLSIEILAGQNLPLppgd-------tkeSGFRPYVSCYLHVECpdeengfpp-ggdnttdseKTSYKRSIKSATGrNP 575 Aspergillus fum...
EEU35876     369 ARDISITIFSGFDIPDdaddeqy-hirrdkSVLRPQVKVEFHCDQde---------------gdKVEHRFKTETGKTkDP 432 Nectria haemato...
Feature 1                                                # #      # #                            
1DJX_B       551 RW------DMEFEFEVt-----------vPDLALVRFMVEDYDsss-knDFIGQSTIPWnslkqGYRHVHLLSkngdqhp 612 Norway rat
EEU42658     541 DFq-----EDHLSFPHvne--------vlEELSWVRFQVMDEGfk--wdELAGWACIRLdrlgnGYRFVHLLDskgm-pt 604 Nectria haemato...
EEU46167     551 NFs-----GERLDFPPvph--------vvDELSWVRFTIRDDEig--kdDLAAWACVRLdrlgqGYRFVHLMDcegr-vt 614 Nectria haemato...
EEU47360     648 DFgp---sGSTLEFLNtpr--------vvEELSFVRFKIEDDSsklgssPLLAWACIRLdrlrpGYRLIRLMDstchavp 716 Nectria haemato...
XP_001222548 606 DWg-----REAKSLDFfgvk------qvvEELSFVRFKVSDGSgnf-ssDVVAWSCIRLdrlqqGYRCINLFHpakrspl 673 Chaetomium glob...
XP_001545984 413 DFe-----GETLHFEGirn--------vvEELSFVRFKIEDANys--kdELAAWACIRLdrlqqGYRLIPLKDikgn-st 476 Botryotinia fuc...
EEH43765     595 DFg-----VQVLQFLSvpv--------slEELSFLRVKVKDDEig--rdDLAAWACIRLdrlrqGYRFIRLFDtkgr-es 658 Paracoccidioide...
AAZ23804     624 NWegkfgaQTELKFENipg--------vvPELSFVRFLVLDDEig--rdSLAAWACVRLdrlreGYRFVHLLDakgm-es 692 Neurospora crassa
EDP52946     576 DFgaqmiqFPTLQGVI-------------DELTFVRFKVKDDElg--rdSLAAWACLKLsrlqqGYRLIHLHDcsga-ea 639 Aspergillus fum...
EEU35876     433 RFga---tGSTTSFDIgwsppdatqivyqELTCFIRFKVVDVQkslaapPLLGWACIRLdrlreGYRLIELSDargr-vt 508 Nectria haemato...
Feature 1                   
1DJX_B       613 sATLFVKISIQ 623 Norway rat
EEU42658     605 eGGILVKVEKI 615 Nectria haematococca mpVI 77-13-4
EEU46167     615 eGVVLVKIDKK 625 Nectria haematococca mpVI 77-13-4
EEU47360     717 gGKLFVKIDKT 727 Nectria haematococca mpVI 77-13-4
XP_001222548 674 eGKLFVKIKKV 684 Chaetomium globosum CBS 148.51
XP_001545984 477 eGLLLVKIEKI 487 Botryotinia fuckeliana B05.10
EEH43765     659 pGILLVKISKQ 669 Paracoccidioides brasiliensis Pb18
AAZ23804     693 dGAVLVKISKK 703 Neurospora crassa
EDP52946     640 gAVLLVRITKV 650 Aspergillus fumigatus A1163
EEU35876     509 sGVLLVKISID 519 Nectria haematococca mpVI 77-13-4

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