1CJY


Conserved Protein Domain Family
cPLA2_like

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cd00147: cPLA2_like 
Click on image for an interactive view with Cn3D
Cytosolic phospholipase A2, catalytic domain; hydrolyses arachidonyl phospholipids
Catalytic domain of cytosolic phospholipase A2 (PLA2; EC 3.1.1.4) hydrolyzes the sn-2-acyl ester bond of phospholipids to release arachidonic acid. At the active site, cPLA2 contains a serine nucleophile through which the catalytic mechanism is initiated. The active site is partially covered by a solvent-accessible flexible lid. cPLA2 displays interfacial activation as it exists in both "closed lid" and "open lid" forms. Movement of the cPLA2 lid possibly exposes a greater hydrophobic surface and the active site. cPLA2 belongs to the alpha-beta hydrolase family which is identified by a characteristic nucleophile elbow with a consensus sequence of Sm-X-Nu-Sm (Sm = small residue, X = any residue and Nu = nucleophile). Calcium is required for cPLA2 to bind with membranes or phospholipids. Group IV cPLA2 includes six intercellular enzymes: cPLA2alpha, cPLA2beta, cPLA2gamma, cPLA2delta, cPLA2epsilon, and cPLA2zeta.
Statistics
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PSSM-Id: 132835
View PSSM: cd00147
Aligned: 40 rows
Threshold Bit Score: 293.768
Threshold Setting Gi: 164660412
Created: 1-Nov-2000
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 5 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:catalytic mechanism proceeds through a serine-acyl intermediate using Ser-228 (1CJY_A) as the nucleophilic residue
  • Comment:catalytic dyad is formed by Ser and Asp
  • Comment:an oxyanion hole is formed by two Gly residues that polarizes the sn-2 ester
  • Comment:Arg may play a role in substrate stabilization in the cleft

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                  ## 
1CJY_A    144 LRFSMA------------LCDQEKTFRQQRKEHIRESMKKLLgpknse------------glhsaRDVPVVAILGSGGGF 199 human
Q9UVX1    122 IRTASE------------LSQQEKDYIHKRQETTNKNLIDFLskranlsdfd---aksfindnapNHNITIGLSFSGGGY 186 Candida albicans
P39457     32 VRSAAK------------LSTNETSWLEVRRGKTLSALKDFFghvkvgdydvg--ayldkhsgnsSSLPNIGIAVSGGGW 97  Penicillium chryso...
Q08108     47 VREATS------------ISQNESAWLEKRNKVTSVALKDFLtratanfsdssevlsklfndgnsENLPKIAVAVSGGGY 114 baker's yeast
Q9P327     81 LRPASEg-----------LNEGEQSYINKRISKVNSELRSFIsktglnvd--------ldkvvnsSDGPRLGIAFSGGGL 141 fission yeast
Q9UTH5     51 IRSCDSseimvnslprgeLPDLENDFIEKRLSNANEALTTFLqsknttad-------ldlssivgDNGPRLGIAVSGGGW 123 fission yeast
Q8TG06     36 IRNAADg-----------LSPAEKEWLKKRDPITRDALQTFLrrafanvste----itsalfndtENVPKLGIAVAGGGY 100 Candida glabrata C...
P78854     63 ESLSSNe-----------IPSAESEYLSTRSTITNTAMKDFLrnanlpgl--------nadtlsgSEGPSIGIALSGGGL 123 fission yeast
Q9Y7N6     74 LRPASDg-----------LSTGEQEFVDKRVSKVNSALESFIsktglkid--------tksvlndTDGPRLGIAISGGGF 134 fission yeast
O42790     65 LRKAVD------------LSNEEKNWLSIRRKNTIQPMRDLLkranitgfdse--tfmneaanniSQLPNVAIAISGGGY 130 Neurospora crassa ...
Feature 1     #                                      #                                        
1CJY_A    200 RAMVGFSGVMKALYE-----------SGILDCATYVAGLSGSTWYMSTLYSHpdfpe-----kgpeeINEELMKNvshnp 263 human
Q9UVX1    187 RAMLAGAGQILGLDGryeda-nkhglGGLLDSSTYVVGLSGGNWLVGSLALNdwlsvg---divngkSTIWQLQDsilnp 262 Candida albicans
P39457     98 RALMNGAGAVKAFDSrtdnatatghlGGLLQSATYISGLSGGSWLLGSIYINnfttvdk--lqtheaGSVWQFGNsiieg 175 Penicillium chryso...
Q08108    115 RSMLTGAGVLAAMDNrtega-yehglGGLLQSTTYLSGASGGNWLVGTLALNnwtsvqdilnnmqndDSIWDLSDsivtp 193 baker's yeast
Q9P327    142 RAMVNGGGAFNAFDSrfes---dsplSGLLQSAMYISGLSGGSWLVGSVAINnftni------tylrDNVWNLEHsvfap 212 fission yeast
Q9UTH5    124 RSMLFGGGALAALDSrsne----ttlGGLLQSAHYITGADGGSWLLSSLAVNefrti------qnisKSIWYTRLgiffi 193 fission yeast
Q8TG06    101 RAMFVGAGAFAAMDNrtdga-nehglGGLLQAATYMAGLSGGNWLTGTLAYNnftsvqqileegdkaDAIWNITNsflnp 179 Candida glabrata C...
P78854    124 RAMILGSGALSAMDArhdn---htvlTGLLQASDYLVGTDGSAWTVGGIALNnfst-------indfSKLWAFNHplmyp 193 fission yeast
Q9Y7N6    135 PAMLTGAGAINAFDArngn---ttslGGILQSSMYLTGLSGGSWLVGSVAVNnfani------tflhDDVWNLDHslfap 205 fission yeast
O42790    131 RALMNGAGFVAAADNriqnttgaggiGGLLQSSTYLAGLSGGGWLVGSLFSNnfssie----tllseNKVWDFENsifkg 206 Neurospora crassa ...
Feature 1                                                                                     
1CJY_A    264 ------llllTPQKVKRYVESLWKKKSSGQPVTFTDIFGMLIGETLIH---------NRMNTTLSSLKekvntAQCPLPL 328 human
Q9UVX1    263 s---gmridkTIAYYYGLAQAVQAKEDAGFQTSVTDTWGRALSYQFFEeddsgtggaNITWSSIRNLSsf-qdHSMPYPI 338 Candida albicans
P39457    176 pdaggiqlldSAGYYKDLADAVDGKKKAGFDTTLTDIWGRALSYQMFNasn---gglSYTWSSIADTPef-qdGDYPMPF 251 Penicillium chryso...
Q08108    194 g---ginifkTAKRWDHISNAVESKQNADYNTSLADIWGRALAYNFFPslnr--ggiGLTWSSIRDFPvf-qnAEMPFPI 267 baker's yeast
Q9P327    213 h---gdnvieNLNYYNDLRKEIDQKKHAGFDCSLTDLWGRALSRKLVDaer---ggpGITYSSMRNQSwf-qnADYPYPI 285 fission yeast
Q9UTH5    194 e---ethfgdLKNYYTNVVDEVNQKAAAGFNVSLTDYWGRAIARHFVGqlr---ggpNLTYSSVQNASwf-qtAEYPYPL 266 fission yeast
Q8TG06    180 y---dkdfskTLARWTAIGSQVQGKRDAGFNVTITDLWSRALAYGWFPtlpn--agaGLTWSSLRDNEif-mnGEMPMPI 253 Candida glabrata C...
P78854    194 k----saivfNAHFYSSIMNEVAEKANAGFNISLSDYWGRVISRTLGDtty---gfpNVSLSSITSQEwy-rnANFPYPI 265 fission yeast
Q9Y7N6    206 y----ddafeNFYIYQEWFEQVLQKKNAGFNVSITDLWGRALALKLVNplt---ggaNTTFSSVTNETwf-qdGEFPFPI 277 fission yeast
O42790    207 pkeaglstvnRIQYWSEVAKEVAKKKDAGFETSITDYWGRALSYQLIGadm---ggpAYTFSSIAQTDnf-qkAETPFPI 282 Neurospora crassa ...
Feature 1                                                                                     
1CJY_A    329 FTCLHVkpdv-----selmFADWVEFSPYEIGMAK--YGTFMAPDLFGSKFFMGTVvk----kyEENPLHFLMGVWGSAF 397 human
Q9UVX1    339 VVANGRtpgty----iineNSTIFEISPYELGSWDpsLKSFSNIQYLGSSVNNGNPnntdicvnNFDNAGFIMGTSSSLF 414 Candida albicans
P39457    252 VVADGRnpgel----vigsNSTVYEFNPWEFGTFDptIFGFVPLEYLGSKFEGGSLpsnescirGFDSAGFVIGTSSSLF 327 Penicillium chryso...
Q08108    268 SVADGRypgtk----vinlNATVFEFNPFEMGSWDpsLNSFANVKYLGTNVSNGVPlergkctaGFDNAGFIMGTSSTLF 343 baker's yeast
Q9P327    286 IVADSRleeet----aipaNTSIFEFTAYEFGTWDngIKAFIPMEYVGTHLLDGVPpdks-cihNYDNAGFVMGTSATLF 360 fission yeast
Q9UTH5    267 IVTQGLtgglpdgsngtatNSSIYEISPYYLTSFDnnVRSYTPTQYLGTNYSNGTAvdgk-cvtQFDNVGFLVGTSSTRY 345 fission yeast
Q8TG06    254 SVADGRypgtt----vinlNATVFEMTPFEIGSWDpsLNAFSDIKYLGTQVTDGKPeter-cinGFDDASFIMGTSSSLF 328 Candida glabrata C...
P78854    266 ITFATQnyged----isnvNTTFFEASPNVFGTFDhgINSFIPTEYLGTTLNNGASsngs-cviNYDNFGFMMGASSTYF 340 fission yeast
Q9Y7N6    278 IIADNVieget----viplNDTVFEFTPIEFGTWDtgVESFIPMEYTGTHLINGIPlnes-cvrNFDNAGFLMGTSSNVF 352 fission yeast
O42790    283 LVADGRapgdt----iislNATNYEFNPFETGSWDptVYGFAPTKYLGANFSNGVIpsggkcveGLDQAGFVMGTSSTLF 358 Neurospora crassa ...
Feature 1                                                                                     
1CJY_A    398 SILFNRvlgvsgsqsrgstmeeelenittkhivsndssdsddeshepkgtenedagsdyqsdnqaswihrmimalvsdsa 477 human
Q9UVX1    415 NQILLQldnysinsiikmil------------------------------------------------------ekvltd 440 Candida albicans
P39457    328 NQFLLQinttslpsfikdvf------------------------------------------------------ngilfd 353 Penicillium chryso...
Q08108    344 NQFLLRinsthlpsfitrla------------------------------------------------------rhflkd 369 baker's yeast
Q9P327    361 NSFLLDwnenvkkndtyydi------------------------------------------------------lhaile 386 fission yeast
Q9UTH5    346 NEALIDvslrqsrmsrrlg---------------------------------------------------------ftlr 368 fission yeast
Q8TG06    329 NEFTMSndsavaytylntls------------------------------------------------------stlvkg 354 Candida glabrata C...
P78854    341 NKIMRNfndsstkngriiq--------------------------------------------------------qylkg 364 fission yeast
Q9Y7N6    353 SGILPAtnasltasnntfnna-----------------------------------------------------vlsfle 379 fission yeast
O42790    359 NQFLLAnissydgvpdvliea----------------------------------------------------vtsvlke 386 Neurospora crassa ...
Feature 1                                                                            #        
1CJY_A    478 lfntregrAGKVHNFMLGlnlntsyplsplsdfatqdsfdddeldaavadpdeferiyepldVKSKKIHVVDSGLTFN-L 556 human
Q9UVX1    441 vsdeeydiAVYEPNPFFGadsag-----------------------------------iksiTTNDTLYLCDGGEDLQnV 485 Candida albicans
P39457    354 ldksqndiASYDPNPFYKyneh------------------------------------sspyAAQKLLDVVDGGEDGQnV 397 Penicillium chryso...
Q08108    370 lsqdfndiAVYSPNPFKDtkflds--------------------------------dyttsiVDSDSLFLVDGGEDDEnV 417 baker's yeast
Q9P327    387 dlskhqddIAPYPNPYQNyttsnt--------------------------------svvnafEPYDTIDLVDGGEDREnI 434 fission yeast
Q9UTH5    369 hmringssVSFYPNPYTDatdiagna-----------------------------tavsediVDTPYLDLFDGGYDGQnI 419 fission yeast
Q8TG06    355 idkenndiAMYAPNPFKGskyvds--------------------------------nyttsiVDSDSLFLVDGGEDLQgI 402 Candida glabrata C...
P78854    365 nfsengqqIISIPNPFQGvesans--------------------------------daannlGSSSSLNLVDTFLTGEkI 412 fission yeast
Q9Y7N6    380 mlaedqldVGLYPNPYQGygnasnt-------------------------------tttnplEPYPIIELIDGGSDSEgI 428 fission yeast
O42790    387 igakrddvSQIIPNPFLDwnnr------------------------------------tnpnADTLELDLVDGGEDLQnI 430 Neurospora crassa ...
Feature 1                                                                                     
1CJY_A    557 PYPlilrPQRGVDLIISFDFSarpsd--ssppfKELLLAEKWAkm------nKLPFPKIDpyvf-dreGLKECYVFKPKn 627 human
Q9UVX1    486 PFYpliqNKRGVDVIFAFDNSadtnss-wpngtSIQETYKRQFskq----gkGTPFPFAPdyktfldkNMGDKPVFFGCn 560 Candida albicans
P39457    398 PLHpliqPERHVDVIFAVDSSadtdyf-wpngtSLVATYERSLnssg--ianGTAFPAVPdqntfinlGLSTRPSFFGCd 474 Penicillium chryso...
Q08108    418 PVLpliqKERDVDIIFAVDNSadmrla-wpdgsSLVHTYERQFvkq----gqGMSFPYVPdtntfvnlGLNKKPTFFGCd 492 baker's yeast
Q9P327    435 PLWpllhPQRFVDVVFAIDSTyndpyg-wplgsSIVATYERVVtfnanksvdVRGFPYIPdentiislGLNTRPTFFGCd 513 fission yeast
Q9UTH5    420 PIWpllqPERKLDVVFAFDSSgdtsnf-wpngsSLVATYERVTqrasdavydVEDFVHVPtpetfvnlGLNANPTFFGCd 498 fission yeast
Q8TG06    403 PFVpllkQERDLDIIFAIDVDtetsdn-ypaggPMMKTYERQFskq----gkGMAFPYVPdmttfvnlGLGGKPSFYGCd 477 Candida glabrata C...
P78854    413 PLWpllqKGRDVDVIVAVDNGddsewl-wpngnSLVQTYERVVaaqaagntnVKGFPYVPsqqsfvslHFNDRPVFFGCd 491 fission yeast
Q9Y7N6    429 PFWpllhPQRDVDVIFAIDGGyqsatsgwpdgsSLVSTYERVLatn---ssgVRGFPYIPdtntflalGLNTHPTFFGCd 505 fission yeast
O42790    431 PLNpltqPVRAVDVIFAVDSSadvtn--wpngtALRATYERTFgsi----snGTLFPSIPddwtfinlGLNNRPSFFGCd 504 Neurospora crassa ...
Feature 1                                                                                     
1CJY_A    628 pdm--------------ekdCPTIIHFVLAninfrkykapgvpreteeekeiadfdifddpeSPFSTFNFQYPNQAFKRL 693 human
Q9UVX1    561 ssdledlvawhendkinvtdVPLVVYTSNTrms---------------------------ynSNFSTFKLSYSDQEKFGA 613 Candida albicans
P39457    475 ssn-------------qtgpSPLVVYIPNApys---------------------------yhSNISTFQLSTDDAERDNI 514 Penicillium chryso...
Q08108    493 annlt----------dlqyiPPLVVYLPNAeys---------------------------fnSNQSAFKLSYSESQRRSM 535 baker's yeast
Q9P327    514 gknttag-----nhdvdnntPPLLVYFPNYpwt---------------------------yySNISTFTMSMDDKMANGI 561 fission yeast
Q9UTH5    499 grnttrg-----dvpvdhntPPLVVYMPNTpwt---------------------------mkSNLVDHRYRIANSEIQAL 546 fission yeast
Q8TG06    478 annlt----------dleyiPPLIVYIPNSyhs---------------------------feSNVSTFKLNYNYSERVGM 520 Candida glabrata C...
P78854    492 grnttag-----nhtvtrdtPPLVIYLPNVpyn---------------------------yfTNISTDRTYYTEDMIQQL 539 fission yeast
Q9Y7N6    506 grnttag-----nhtvnddtPPLVVYFPNYpwt---------------------------myANVTTYTVQLEDTLSSGM 553 fission yeast
O42790    505 vknftl--------nanqkvPPLIVYVPNApyt---------------------------alSNVSTFDPSYTMSQRNDI 549 Neurospora crassa ...
Feature 1                             
1CJY_A    694 HDLMHFNTLn---NIDVIKEAMVE 714 human
Q9UVX1    614 IRNGFETVTrnnlTDDENWSTCVG 637 Candida albicans
P39457    515 ILNGYEVATmansTLDDNWTACVA 538 Penicillium chrysogenum
Q08108    536 IQNGFEIATrnnfTDDPEFMGCVG 559 baker's yeast
Q9P327    562 LENAFMSTTq---NNNESFAVCLA 582 fission yeast
Q9UTH5    547 IQNGFVATTq---DNSTDFASCLA 567 fission yeast
Q8TG06    521 IRNAFEATTrnnlTEDADYVTCVG 544 Candida glabrata CBS 138
P78854    540 LTNGLISSTv---DNDTYFGQCFA 560 fission yeast
Q9Y7N6    554 IENAAVAATq---NNSDSFAVCVA 574 fission yeast
O42790    550 IGNGWNSATqgngTLDSEWPTCVA 573 Neurospora crassa OR74A

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