1TVM,2R48,1VKR,1H9C,1E2B,1IIB,1VRV


Conserved Protein Domain Family
PTS_IIB

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cd00133: PTS_IIB 
Click on image for an interactive view with Cn3D
PTS_IIB: subunit IIB of enzyme II (EII) is the central energy-coupling domain of the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS). In the multienzyme PTS complex, EII is a carbohydrate-specific permease consisting of two cytoplasmic domains (IIA and IIB) and a transmembrane channel IIC domain. The IIB domain fold includes a central four-stranded parallel open twisted beta-sheet flanked by alpha-helices on both sides. The seven major PTS systems with this IIB fold include chitobiose/lichenan, ascorbate, lactose, galactitol, mannitol, fructose, and a sensory system with similarity to the bacterial bgl system. The PTS is found only in bacteria, where it catalyzes the transport and phosphorylation of numerous monosaccharides, disaccharides, polyols, amino sugars, and other sugar derivatives. The four proteins (domains) forming the PTS phosphorylation cascade (EI, HPr, EIIA, and EIIB), can phosphorylate or interact with numerous non-PTS proteins thereby regulating their activity.
Statistics
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PSSM-Id: 99904
View PSSM: cd00133
Aligned: 454 rows
Threshold Bit Score: 30.687
Threshold Setting Gi: 150017562
Created: 2-May-2007
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 5 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:most of the active site residues are contained within a P-loop which forms part of a binding pocket for the phosphoryl group
  • Comment:for the E coli chitobiose transporter IIB subunit, in addition to the P-loop residues, two conserved residues are likely to be important in catalysis: a tyrosine whose side-chain points towards the phosphorylation site and a Gln residue whose side-chain lies close to the sulfur atom of the catalytic cysteine.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1             # ##  ##                                                                   
1TVM_A        23 KIIVACGGAvATSTMAAEEIKELCQSHNIp-VELIQCRvneietymDGVHLICTTarvdrsfgDIPLVHGmpfvsgvgie 101 Escherichia coli
NP_621994    412 NVLVVCSTGiGTAKLLSSRLQSVFDVHII--DTIAFHQikev-lkdKKIDLIVSTipl--kcdEVKVVEVnpll----td 482 Thermoanaerobac...
YP_001254646 407 NVLIVCATGiGTSKFISNKLKSIFDINII--DTISSHTmekilkynKNIDLIVTTipl--kvkGIKCIEVntfl----te 478 Clostridium bot...
NP_622264    416 NILVVCASGiGTSRMLMSKLQMFPQLNVV--EVASSVKlkel-ekrKDIDLIVSTipl--kltSKKVVVVnpll----le 486 Thermoanaerobac...
YP_001485473 405 KAIIVCGSGlGTARLLEIKLKTAFQHELDivELYSYQDylm--sttLPCDVIITTvpl--askGKPVIQVsaff----eq 476 Bacillus pumilu...
EBA32867     409 SALIVCGSGfGTARIVEAKVKSAVSNLDIt-KVVSIQEynr--fihIEEDIILSTvri--pekNKPVIKIsnip----tn 479 Listeria monocy...
EDP68758     411 RAVVVCTTGlGTSVLLKAKLQSKTNLNIKvvGVYALYQldd--iefTDIDIIISTvpls-kkySIPDVVItpiv----sn 483 Carnobacterium ...
ZP_01801941  401 KICIVCHYGiGTGQLISEKLKQNISDLSVv-GVYPVRYldm--aisQDVDLIVSTvel--kgyEKPVLYIenif----dd 471 Clostridium dif...
CAL13801     407 QVMLVCDTGnSTTRMIQAQISRKYPQIVMt-QTISLRDyen--ldhIDEDFIISNsrl--tekNKPVVVMspfp----te 477 Yersinia entero...
YP_858357    402 QALLLCDAGnATFRVLEARIKREYPQLQLt-MLESVRDyeg--larIEQDFVISTvka--gekNVPVVQVapfp----tq 472 Aeromonas hydro...
Feature 1                  
1TVM_A       102 alQNKILTIL 111 Escherichia coli
NP_621994    483 rdIEKLSKFL 492 Thermoanaerobacter tengcongensis MB4
YP_001254646 479 knISELGLYF 488 Clostridium botulinum A str. ATCC 3502
NP_622264    487 edVEKLKKAL 496 Thermoanaerobacter tengcongensis MB4
YP_001485473 477 hdVQQVNEVI 486 Bacillus pumilus SAFR-032
EBA32867     480 eeLKMLGAII 489 Listeria monocytogenes FSL N3-165
EDP68758     484 ndIEKIHEFI 493 Carnobacterium sp. AT7
ZP_01801941  472 slIENVNKAF 481 Clostridium difficile QCD-32g58
CAL13801     478 yqLEQLGKLV 487 Yersinia enterocolitica subsp. enterocolitica 8081
YP_858357    473 yqLEQLGKLV 482 Aeromonas hydrophila subsp. hydrophila ATCC 7966

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