1BK9,1POE,1A2A,2NOT,1BUN,1PSH,1OZY,1LE6


Conserved Protein Domain Family
PLA2c

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cd00125: PLA2c 
Click on image for an interactive view with Cn3D
PLA2c: Phospholipase A2, a family of secretory and cytosolic enzymes; the latter are either Ca dependent or Ca independent. PLA2 cleaves the sn-2 position of the glycerol backbone of phospholipids (PC or phosphatidylethanolamine), usually in a metal-dependent reaction, to generate lysophospholipid (LysoPL) and a free fatty acid (FA). The resulting products are either dietary or used in synthetic pathways for leukotrienes and prostaglandins. Often, arachidonic acid is released as a free fatty acid and acts as second messenger in signaling networks. Secreted PLA2s have also been found to specifically bind to a variety of soluble and membrane proteins in mammals, including receptors. As a toxin, PLA2 is a potent presynaptic neurotoxin which blocks nerve terminals by binding to the nerve membrane and hydrolyzing stable membrane lipids. The products of the hydrolysis (LysoPL and FA) cannot form bilayers leading to a change in membrane conformation and ultimately to a block in the release of neurotransmitters. PLA2 may form dimers or oligomers.
Statistics
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PSSM-Id: 153091
View PSSM: cd00125
Aligned: 59 rows
Threshold Bit Score: 83.068
Threshold Setting Gi: 115727506
Created: 1-Nov-2000
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 6 residues -Click on image for an interactive view with Cn3D
Feature 1:catalytic network [active site]
Evidence:
  • Structure:1POE; human secretory phospholipase A2 bound with transition-state analog (1-o-octyl-2-heptylphosphonyl-sn-glycero-3-phosphoethanolamine), contacts at 3.5 A.
    View structure with Cn3D
  • Comment:a key bound water molecule is part of the catalytic network
  • Citation:PMID 1948070
  • Citation:PMID 9276469
  • Citation:PMID 9013608

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                         #                    ##  #                   # 
1BK9           1 SLIQFETLIMKVAk----kSGMf-WYSNYGCYCGWGG--QGRP-QDATDRCCFVHDCCYGKVt---GCD----PKMDVYS 65  halys viper
1POE_A         1 NLVNFHRMIKLTTg----kEAAl-SYGFYGCHCGVGG--RGSP-KDATDRCCVTHDCCYKRLek-rGCG----TKFLSYK 67  human
1BUN_A         1 NLINFMEMIRYTIpc--ekTWG--EYADYGCYCGAGG--SGRP-IDALDRCCYVHDNCYGDAekkhKCN----PKTQSYS 69  many-banded krait
AAZ98832      33 TIFQLCTLINYYTn----rTCT--DYNDYGCYCGYGQa-VGKP-RDYADACCRKHDNCYGRAv--eSCYl--fPQIVSIG 100 edible mussel
ABA43328      17 SALNFQSMISYTTg----rSAL--AFNGYGNWCGLGPyfSTPPtVDSVDACCKAHDNCYSDT----GCNllqwEVLNPYD 86  Trichoplax sp. ...
NP_651686     47 DVARLYSMIKCSTg----cDPL--IYKGYGCYCGFGG--HGVP-ADGIDRCCRVHDKCYGQS----NCIsy-lEYFVPYV 112 fruit fly
XP_001177297  25 NVISLGSMLGWLDpklnsiFGAygRFNRYGCYCGIGG--SGLP-VDQIDCCCQLHDACYDSLtddgTCTrg-gTFSHLYQ 100 purple urchin
NP_496228     25 ALWNLEEVAECELh----yNAL--HYNNYGCWCGIGG--SHEP-VDGIDECCMHHDKCYDAAvdnkVCMdveiEYVDDYS 95  nematode
EAT45131      45 AVYNLYSMIKCATg----cDPI--IYKGYGCYCGFLG--SGQT-VDGIDRCCKMHDYCYSKA----KCLmf-lEYFVPYL 110 yellow fever mo...
NP_493719     19 FVLEIQFMTRCMTh----hDAW--IYNGYGCYCGIGG--SGDI-LDDIDECCANHDKCYEDLdlkkTCWttpfEYFPIYS 89  nematode
Feature 1                                             #                             
1BK9          66 FSEE----NGDIVCGgd----------dPCkKEICECDRAAAICFRDNltlYNDk-KYWAfg-akNC 116 halys viper
1POE_A        68 FSNS----GSRITCAkq----------dSCrSQLCECDKAAATCFARNkttYNK--KYQYys-nkHC 117 human
1BUN_A        70 YKLT----KRTIICYga---------agTCaRIVCDCDRTAALCFGNSe--YIE--GHKNidtarFC 119 many-banded krait
AAZ98832     101 YTCD----DNTRQCRctd-------avnTCeRTVCECETSLAHCLSRAp--YHNtyYHMDr---sEC 151 edible mussel
ABA43328      87 WHKSs---TGRITCDda---------pgSCdRANCECDRIAAECFARYk--YNC--DYAPlsyklFC 137 Trichoplax sp. BZ10101
NP_651686    113 WKCY----RGKPLCAvdhge---fggpdSCaARLCQCDLRLSRCLKRY---YCP--HRRS-----IC 162 fruit fly
XP_001177297 101 YTKYkedgRPQIKCKes---------ddPCaYKLCQCDRKVAVCLSTHeskYNMkyKNYDrn--tKC 156 purple urchin
NP_496228     96 WSCM----NSTAICSdk---------nmGCkAALCDCDKIVVECWKKF---PKP--EKKA-----KC 139 nematode
EAT45131     111 WKCY----KGRPLCAidhge---wngpnACaSRLCHCDLSLSKCLRRY---HCP--RKRN-----VC 160 yellow fever mosquito
NP_493719     90 WKCQ----NQTIHCDtlseasdhpvlahECsNQLCECDRKLVECWAKY---PEP--TGQL-----KC 142 nematode

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