Conserved Protein Domain Family
EgtA_Cys_ligase
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TIGR03444: EgtA_Cys_ligase 
ergothioneine biosynthesis glutamate--cysteine ligase EgtA
Members of this bacterial protein family, EgtA, resemble the glutamate--cysteine ligase of the two-step pathway for glutathione (GSH) biosynthesis, but instead are involved in the biosynthesis of the histidine-derived thiol, ergothioneine (EGT). Successful in vitro reconstitution of EGT biosynthesis using EgtBCDE and gamma-L-glutamyl-L-cysteine suggests that this enzyme is a bone fide glutamate--cysteine ligase. [Biosynthesis of cofactors, prosthetic groups, and carriers, Glutathione and analogs]
Links
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Statistics
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PSSM-Id: 274583
Aligned: 8 rows
Threshold Bit Score: 461.6
Created: 8-Oct-2014
Updated: 25-Oct-2021
Structure
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Aligned Rows:
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
WP_011607875  83 ETSAGPEPSVGIGTsVGIETEwfvv-------dtrvVHQPVPPERTRR-ALGRISGLITGAagPVLPGGSRLTFEPGGQL 154 Frankia alni
WP_031696365  32 YIADGCLVDGPLGR-VGLEME---------------AHCFDPADPFRRpSWEEITEVLEWL--SPLPGGSVVSVEPGGAV 93  Mycobacterium t...
WP_011438865  72 DTRGNGGPTVPGGC-VGIETEwliv-------dtqaVHCLIPPARTLA-SLARIPGTTPGPggPVLPGGSRITFEPGGQL 142 Frankia sp. CcI3
WP_014878601  28 HIGANSLQDGPIGR-VGLEIE---------------AHCFDLSNPTRRpSWDELSAVIADV--PPLPGGSRITVEPGGAV 89  Mycobacterium s...
WP_011562258  27 HITEHCLHDGPVGQ-VGLEVE---------------AHSFDLADPRRRpDWGEVTDAIAGVg--aLPGGSRITVEPGGAV 88  Mycobacterium
WP_011597826  22 YLGGVCFKLGPPQL-IGAELEwlta-------qlpeYVRPDLAALAEAlGPHTPRTIDPTSpaQPLPGGSAVTVEPGGQI 93  Rhodococcus jostii
WP_011027438  23 LVRGICFKTGPPRL-LGVEVEwl---------------vhELRAPRLPvSPERLQAVYTAL--RAVPLRSALTVEPGGQL 84  Streptomyces
WP_011211422  28 FVGGVCFKLGPPAL-IGAELEwltvqggpsasatvaAPRPQLSALAAAlGPHAPRSIAPDSpaLPLPGGSRITVEPGGQI 106 Nocardia farcinica

WP_011607875 155 ELSGPPA-GLAAAVQSTAADLHLVRAALAEDGLAIVGMGVDPLRAGVRQTTASRYAAMEEHF-R-TGGGTSGEVMMRSTA 231 Frankia alni
WP_031696365  94 ELSGPPAdGVLAAIGAMTRDQAVLRSALANAGLGLVFLGADPLRSPVRVNPGARYRAMEQFF-AaSHSGVPGAAMMTSTA 172 Mycobacterium t...
WP_011438865 143 ELSGPPA-ELTAAVRASSADLNLVRSALAVDGLALVGMGVDPLRVGMRQTTASRYAAMEEHF-R-TGGGASGTVMMYSTA 219 Frankia sp. CcI3
WP_014878601  90 ELSGPPYdGPLAAVAALQADRAVLRAEFARRNLGLVLLGTDPLRPTRRVNPGARYSAMEQFFtA-SGTAEAGAAMMTATA 168 Mycobacterium s...
WP_011562258  89 ELSGPPAdGSVAAIAAMTSDRAVLRTAFAQRGLGLVLVGADPLRRPRRVNPGGRYSAMEAFFtA-SGTGAAGAFMMTSTA 167 Mycobacterium
WP_011597826  94 ELSSAPYySARELCDYLAADQAVLTDLLSSRSILMSSEAADTRRPAQRLLQSPRYCAMESRF---TGIGPFGKLMMCNTA 170 Rhodococcus jostii
WP_011027438  85 ELSSLPAaSLTECVRSVSADLEAVRAVLREDGLALVGLGHDPWHPPRRYLRQPRYDAMESCLdR-TGPG--GRFMMCASA 161 Streptomyces
WP_011211422 107 ELSSAPYaEAAELCARLREDAIVLREMLAAAAIRPLALAADADRRAKRLLRSPRYRAMEQSF-A--GIGPFGKLMMCNTA 183 Nocardia farcinica

WP_011607875 232 SVQVNLDLGt---tPAQ-AAERFRLAHAVGPVLTAMFAASPALAGRRTG-WRSTRQAVWSGIDAGRT--GSVLDLPAqpa 304 Frankia alni
WP_031696365 173 AIQVNLDAG-----PQEgWAERVRLAHALGPTMIAIAANSPMLGGRFSG-WQSTRQRVWGQMDSARC--GPILGASG--- 241 Mycobacterium t...
WP_011438865 220 SVQVNLDLGg---sPAE-AARRFQLVHALGPVLTALFAASPAYAGRRTG-WRSTRQAVWDGIDASRT--RPVAAALT--- 289 Frankia sp. CcI3
WP_014878601 169 SVQVNLDAG-----PRDgWAERVRLAHALGPTMIAITANSPMLGGQFTG-WCSTRQRVWGQLDSARC--GPVLGVDG--- 237 Mycobacterium s...
WP_011562258 168 SIQLNLDAGp----rRG-WADRVRLAHALGPTMIAATANSPLLAGRFSG-WQSSRQWVWGELDEARC--GPVLGASG--- 236 Mycobacterium
WP_011597826 171 ATQISVDAGa---dPVA-VEKRWHLLHAIGPALIAAFACSPRLYGVPDGqWASQRMRTWLELDSPRTigTPHVAD----- 241 Rhodococcus jostii
WP_011027438 162 SVQVCVDAGheepgPLG-HVRRWWLAHHLGAVLLAAFANSPLSGGRPTG-WRSTRQLRWTQIGAGRAg-GPPLDS----- 233 Streptomyces
WP_011211422 184 ATQVSVDAGt---dPAE-VRARWTALYTMGPALVAAFACSPALRGAPPGrWASQRMRTWLRLDTLRT--RPPVADWA--- 254 Nocardia farcinica

WP_011607875 305 aggdagpgaaaarsG--------DLADRWARYLLAAKLMMIGDPlgdvgpgE--------DRRFVAATGGHTFGDWVAG- 367 Frankia alni
WP_031696365 242 --------------D--------HPGIDWAKYALKAPVMMVRSP-------D--------TQDTRAVTDYVPFTDWVDG- 283 Mycobacterium t...
WP_011438865 290 --------------ApgsgdtepDLVDLWARYLLDAKLMMVENParagnpeD--------DHHFVPVRDDSTFGDWLAG- 346 Frankia sp. CcI3
WP_014878601 238 --------------D--------DPASEWARYALRAPVMLVNSP-------D-----------AVPVTNWVPFADWADG- 276 Mycobacterium s...
WP_011562258 237 --------------D--------DPASDWARYALRAPVMLVHNP------------------EPVPVTDWVPFAEWADG- 275 Mycobacterium
WP_011597826 242 ----------------------------YAGWVLDVPLLCIRN-----------------DGTDWTAPDGATFADWVEGa 276 Rhodococcus jostii
WP_011027438 234 -----------------------DPRGTWARHVLDAPVMCVR---------R--------DGGPWDVPEGLTLREWSRE- 272 Streptomyces
WP_011211422 255 -----------------------DPITGYARWALDVPLLCVRSV-------DgtttcagdPGENWTAPPGATFADWMAGa 304 Nocardia farcinica

WP_011607875 368 RGPVE-RAPTLDDLSYHATTLFPPVRPRGWWELRYLDAQPA-EGWRVAVAVATALLDDPVAAAAAAQVCAPVARR----- 440 Frankia alni
WP_031696365 284 RVLLDgRRATVADLVYHLTTLFPPVRPRQWLEIRYLDSVPD-EVWPAVVFTLVTLLDDPVAADLAVDAVEPVATA----- 357 Mycobacterium t...
WP_011438865 347 RGPVT-RPPTREDLSYHATTVFPPVRPRGWWELRYLDAQPG-DGWQAAVAMPTVLLDDPVAADGAFEACLAVAHR----- 419 Frankia sp. CcI3
WP_014878601 277 RAVLGgRRPTEADLDYHLTTLFPPVRPRRWLEIRYLDSVPD-ALWPAAVFTLTTLLDDPVAAESAAEATRPVATA----- 350 Mycobacterium s...
WP_011562258 276 QTLLGgRRPTRSDLEYHLTTLFPPVRPRRWLEIRYLDAVSD-ALWPAVAFTLTTLLDDPAAADIAAEATESVATA----- 349 Mycobacterium
WP_011597826 277 LDDVIgR-PTPADLDYHLTTLFPPVRAAGHLEVRYLDAQPG-DQWRVPIAAIDALLSGPGAMAEALDAALPTAER----- 349 Rhodococcus jostii
WP_011027438 273 LVP---RPPTREDLDYHLTTLFPPVRPRGHLELRMIDAQPGdDGWIVPLAVTAALFDDPEATETAYRAVKPLAERtlglp 349 Streptomyces
WP_011211422 305 LDDEIgRRPDPADLAYHLTTLFPPVRPSGHLEVRYLDAQPG-EDWTVPIHTFDALLAHPAVVAEATALAAPLADQ----- 378 Nocardia farcinica

WP_011607875 441 ------WPAAAMLGMADEPLRLAAVrcleLAAAALR----R-----AGCADLADAVELFSeRYPQRGRCPADE 498 Frankia alni
WP_031696365 358 ------WDTAARIGLADRRLYLAAN----RCLAIAA----R-----RVPTELIGAMQRLV-DHVDRGVCPADD 410 Mycobacterium tubercul...
WP_011438865 420 ------WPAAAMLGMADEPLRRAAV----RCVDLAVsalrR-----GGATDLAETVESFAgRYLDRGRCPADE 477 Frankia sp. CcI3
WP_014878601 351 ------WDRAARMGLTDRHLHTAAL----TCVRLAA----E-----RAPAELEESMTLLM-RSVQQRRSPADD 403 Mycobacterium smegmatis
WP_011562258 350 ------WDRAARIGLGDRRLHAAAL----RCVETAA----E-----RAPAAVMESMQQLV-RSVEEGRSPADD 402 Mycobacterium
WP_011597826 350 ------WRDAAEFGLAELELRAAAT----ALLSLAA----A-----YSPDPEFVRLLDAAaERCCRGQAPSEE 403 Rhodococcus jostii
WP_011027438 350 aphnplYEDAARDALTDPELREAAV----TCFTAAL----AalprlGASTEVTDAVAAYReRYVLRGRCPADD 414 Streptomyces
WP_011211422 379 ------WSQAARCGLADGVVRTVAA----ELLDLAA----A-----HARTPEAGAELDRAaRRCRAGRTPTEE 432 Nocardia farcinica
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