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phosphonate metabolism-associated iron-containing alcohol dehydrogenase This small clade of iron-containing alcohol dehydrogenases of the pfam00465 family is found in genomic contexts indicating a role in the metabolism of phosphonates. In Delftia acidovorans SPH-1, the gene ZP_01580650.1 is adjacent to and running in the same direction as ZP_01580649.1 encoding the enzyme phosphonatase (PhnX, TIGR01422). Upstream are also found genes encoding components of a phosphonate ABC transport complex. In Ralstonia eutropha H16 and Verminephrobacter eiseniae EF01-2 the dehydrogenase is followed by a homolog of the PhnB gene, a putative phosphonate-specific MFS-type transporter. In Azoarcus BH72 the gene is preceded by Phosphoenolpyruvate phosphomutase (aepX) and a putative phosphonopyruvate decarboxylase (aepY), two genes involved in the biosynthesis of phosphonoacetaldehyde (Pald). Ususally these two are accompanied by a specific transaminase, AepZ, which converts Pald to 2-aminoethylphosphonate (2-AEP). 2-hydroxyethylphosphonate (2-HEP), the presumed product of the reaction of Pald with an alcohol dehydrogenase, is a biologically novel but reasonable analog of 2-AEP and may be a constituent of as-yet undescribed natural products. In the case of Azoarcus, downstream of the dehydrogenase is a CDP-glycerol:glycerophosphate transferase homolog that may indicate the existence of a pathway for 2-HEP-derived phosphonolipid biosynthesis. |
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