2J2C


Conserved Protein Domain Family
HAD-IG-Ncltidse
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TIGR02244: HAD-IG-Ncltidse 
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HAD superfamily (subfamily IG) hydrolase, 5'-nucleotidase
This model includes a 5'-nucleotidase specific for purines (IMP and GMP). These enzymes are members of the Haloacid Dehalogenase (HAD) superfamily. HAD members are recognized by three short motifs {hhhhDxDx(T/V)}, {hhhh(T/S)}, and either {hhhh(D/E)(D/E)x(3-4)(G/N)} or {hhhh(G/N)(D/E)x(3-4)(D/E)} (where "h" stands for a hydrophobic residue). Crystal structures of many HAD enzymes has verified PSI-PRED predictions of secondary structural elements which show each of the "hhhh" sequences of the motifs as part of beta sheets. This subfamily of enzymes is part of "Subfamily I" of the HAD superfamily by virtue of a "cap" domain in between motifs 1 and 2. This subfamily's cap domain has a different predicted secondary structure than all other known HAD enzymes and thus has been designated "subfamily IG". This domain appears to consist of a mixed alpha/beta fold. A Pfam model (pfam05761) detects an identical range of sequences above the trusted cutoff, but does not model the N-terminal motif 1 region. A TIGRFAMs model (TIGR01993) represents a (putative) family of _pyrimidine_ 5'-nucleotidases which are also subfamily I HAD's, which should not be confused with the current model.
Links
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Statistics
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PSSM-Id: 274052
Aligned: 11 rows
Threshold Bit Score: 459.479
Created: 8-Oct-2014
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
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PubMed ReferencesClick to see Conserved Features Help
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
2J2C_A             54 VFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLVSI-GYPQELLSFAYDSTFPTRGLVFDTLYGNLLKVD 132 human
AAK93139          101 TSYHKELLLKDYGIVGFDLDGTLLRYNLHEMSALIYQVLKQFLVEQkGYDRDLLLQSLNLDFLQKGLFLDGPRGNVLKLS 180 fruit fly
AAP68896           83 IFCNRSLNMRNITAVGFDMDYTLAQYKPETFEALAYHGTIEKLVKDlGYPEELLTWQFDWKYMVRGLVLDKKRGNILKMD 162 Japanese rice
CAE78341            5 VFVNRTLNLKKIRYIGVDMDHTLVRYNSENFERLSHTTMIDKLVKR-GYPETLRKLVFDYNFAIRGLVIDRKMGNLLKLN 83  Bdellovibr...
AAC68740           13 VFVNRSLRLEKITHFGFDMDYTLVQYKAPDMEYLAFNMAVQRLVDT-GYPEEIKSFKYDPIFPVRGLWFEFQFGNLLKVD 91  Caenorhabd...
BAB10319          142 IFCSRTLNLRSISAIGYDMDYTLMHYNVMAWEGKAYDYCMENLKSM-GFP--VDGLAFDPELVIRGLMIDKEKGNLVKAD 218 thale cress
BAB14763           56 IYANNEISLRDVEVYGFDYDYTLAQYAD-ALHPEIFSTARDILIEHyKYPEGIRKYDYNPSFAIRGLHYDIQKSLLMKID 134 human
FlyBase:CG1814-PC  14 VFACNELDLSEVQVYGFDYDYTLACYKP-ILEDLLYNLAREMLVKRfRYPEDILQLEYEPNFAVRGLHYDVEKGLLVKLD 92  fruit fly
AAS38802          148 VFINSELKLEEIDVFGFDYDYTLANYGD-QVQHLIYDLAMSHLVDEqKYPMALKEIKYDPTFAIRGLHFDVNHGLLMKLD 226 Dictyostel...
AAC63660           99 IYVNKNLRLDNIQVYGFDYDYTLAHYSS-HLQSLIYDLAKKHMVNEfRYPDVCTQFEYDPTFPIRGLYYDKLKGCLMKLD 177 thale cress
P49902             35 VFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLVSI-GYPQELLSFAYDSTFPTRGLVFDTLYGNLLKVD 113 human

2J2C_A            133 AYGNLLVCa--HGFNFIRGPETREQYPN------------KFIQRDDTe-------RFYILNtLFNLPETYLLACLVDFF 191 human
AAK93139          181 SEAQILRAt--HGTRLLSDTEIQAIYGRdrrweittafyrDPLSTWNGdas---tqMRSLLD-YFDMPSALVFAQAVDLV 254 fruit fly
AAP68896          163 RHKYVKVAy--HGFREMSKEEKVSAYGS------------TLIRDSFDe------pDYALIDtLFSLGEAYLFAQLVDFM 222 Japanese rice
CAE78341           84 RYTAIRASy--HGLKPLDFKSHQKLYKS------------TYIDLSNS--------DYLAVDtSFSISLANLIAQIVELK 141 Bdellovibr...
AAC68740           92 GFGNILGGi--HGLRFLKNHEIEEMYPN------------KYLPLSDs--------RVYVLNtLFNMPETHLICQLIDFF 149 Caenorhabd...
BAB10319          219 RFGYVKRAm--HGTKMLSNKAVSEIYGR------------ELVDLRNQs-------RWEFLNtFFSVSEALAYAQMVDRL 277 thale cress
BAB14763          135 AFHYVQLGtayRGLQPVPDEEVIELYGGtqh------iplYQMSGFYGkg-----pSIKQFMdIFSLPEMALLSCVVDYF 203 human
FlyBase:CG1814-PC  93 SFLQLQLGsvyRGRTKVEADEVLKLYHNrll-------piAYVEGPNNsyrhntnsKMVQLAdLFSVPEMCLLCNVIEYF 165 fruit fly
AAS38802          227 YLNNIQAGaiyHGRRPLTKEEVIQIYGS------------MQLKRLYCd------sFLKPMSdIFCLPEACLIANTIQYL 288 Dictyostel...
AAC63660          178 FFGSIEPDgcyFGRRKLSRKEIESMYGT------------RHIGRDQAr-------GLVGLMdFFCFSEACLIADMVQYF 238 thale cress
P49902            114 AYGNLLVCa--HGFNFIRGPETREQYPN------------KFIQRDDTe-------RFYILNtLFNLPETYLLACLVDFF 172 human

2J2C_A            192 TNCPrytsce--tgfkdgDLFMSYRSMFQDVRDAVDWVHYKGSLK-------EKTVENLEKYVVK-DGKLPLLLSRMKEV 261 human
AAK93139          255 DRGKk-------------PSAGEEYQVWQDVQAGLMNIYSRENFAndqslyfKSMRAEPERFVLRtDPSVMNWLGELRKS 321 fruit fly
AAP68896          223 DNNPgk-----------vPSGTDYPLMYRDVRSAVDLCHRDGTLK-------RMVAKDPSRYINE-DLAIVPMLEMIRKS 283 Japanese rice
CAE78341          142 DSDt-------------aNKYPEYAQIADDVLDALDEAHRDGSLK-------DVVKQNLDHFIIK-DPTLVHTLEKFRRH 200 Bdellovibr...
AAC68740          150 DKHPhysmltdktgvkggEVMMSYKSIFQDLRRVIDWVHVESTMK-------KQVMENPEKYVIR-DERAPRFLSQLREH 221 Caenorhabd...
BAB10319          278 DDGFis----------adLGTLDYKGLYKAVAKALFRAHVEGQLK-------SEIMSKPELFVEP-DPELPLALLDQKEA 339 thale cress
BAB14763          204 LGH---------------SLEFDQAHLYKDVTDAIRDVHVKGLMY-------QWIEQDMEKYILR-GDETFAVLSRLVAH 260 human
FlyBase:CG1814-PC 166 ERN---------------RIDYNPEIVFHDTRTAMGSCHP--IMH-------GKVMRNTEKYIER-NPKLVKFFEKLQQA 220 fruit fly
AAS38802          289 TDH---------------NLAFEPRIIHEDVTAAVGKVHLGGGLH-------NKIISDFPLYLNK-HPLLGEFLLKLKSH 345 Dictyostel...
AAC63660          239 VDA---------------KLEFDASNIYNDVNRAIQHVHRSGL------------------------GQLLRFLRMLKDK 279 thale cress
P49902            173 TNCPrytsce--tgfkdgDLFMSYRSMFQDVRDAVDWVHYKGSLK-------EKTVENLEKYVVK-DGKLPLLLSRMKEV 242 human

2J2C_A            262 Gk-VFLATNSDYKYTDKIMTYLFDfph------gpKPgsshrpWQSYFDLILVDARKPLFFG-EGTVLRQVDTKTGKLKI 333 human
AAK93139          322 GkkLFLLTGSNVDFANLTATQALG-----------KD------WRKYFDFVVTYAKKPGFFT-MQRPFMQVDVEKLAELP 383 fruit fly
AAP68896          284 GrsTFLVTNSLWDYTDVVMNYLCRpytsdvssshnHK------WLGYFDVVITGSAKPGFFH-DGNRAGLFEVEPDSGKL 356 Japanese rice
CAE78341          201 GkkIFVLTNSDFHYTKLLLDYAIQpflk-----------ehksWEDLFEFVITFASKPKFFY-ENQ--KYLRVNPADGTM 266 Bdellovibr...
AAC68740          222 GkkTFLLTNSDWTYTNVMMKFILG-----------DD------WKTYFNIIVVDSCKPRWFA-EGTVFREVNTDTGSYKL 283 Caenorhabd...
BAB10319          340 GkkLLLITNSDYHYTDKMMKHSFNkfl-----pndMD------WRDLFDMVIVSARKPEFFQ-MSHPLYEVVTGEGLMRP 407 thale cress
BAB14763          261 GkqLFLITNSPFSFVDKGMRHMVG-----------PD------WRQLFDVVIVQADKPSFFTdRRKPFRKLDE-KGSLQW 322 human
FlyBase:CG1814-PC 221 GknLFLVTNSPYSFVNCGMSFLVG-----------AN------WRDFFDVVIVQARKPKFFTdESRPIRLFDERTQSHLW 283 fruit fly
AAS38802          346 GkkLFLLTNNSYFYANHGMKYLLNdqln----gkyED------WTDVFDVIITKCDKPSFFG-KGRPFRMYHPDSDRYDW 414 Dictyostel...
AAC63660          280 GkkLFLLTNSPYNFVDGGMRFLMEesf-----gfgDS------WRELFDVVIAKANKPEFYT-SEHPFRCYDSERDNLAF 347 thale cress
P49902            243 Gk-VFLATNSDYKYTDKIMTYLFDfphgpkpgsshRP------WQSYFDLILVDARKPLFFG-EGTVLRQVDTKTGKLKI 314 human

2J2C_A            334 GTYTGpLQHG-------------IVYSGGSSDTICDLLG-----AKG-KDILYIGDHIFGDILKSKKRQGWRTFLVIPEL 394 human
AAK93139          384 QEADN-IKAG-------------EVYSQGNWHQLHTAMAgmlqkKPSeAKALYFGDNIIQDVYTPVKHRDFDAVAIAEEL 449 fruit fly
AAP68896          357 LNAD--LHIGsprsgqqpsrpihKIYQGGNVGHLHRLLS-----VASsSQILYVGDHIYGDILRSKKVLGWRTMLVIPEL 429 Japanese rice
CAE78341          267 TNMEGkLTPG--------------IYQGGNAKKFTADLD-----LAG-DDILYVGDHIYGDILRLKKDCNWRTAMVIEEL 326 Bdellovibr...
AAC68740          284 GIHTGpLREG-------------VVYSGGSCDAFQKMMK-----CRG-KEVLYVGDHIFGDVLRSKKSRGWRTFLVVPEL 344 Caenorhabd...
BAB10319          408 CFKAE--TGG--------------LYSGGSAQMIESSLN-----VHG-DEILYVGDHIYTDVSVSKVHLRWRTALICREL 465 thale cress
BAB14763          323 DRITR-LEKG-------------KIYRQGNLFDFLRLTE-----WRG-PRVLYFGDHLYSDLADLMLRHGWRTGAIIPEL 382 human
FlyBase:CG1814-PC 284 DRVFK-LEKG-------------KIYYEGSVRQLQELKG-----WRG-HSVLYFGDHPYSDLADVTLKHSWRTGAIISEL 343 fruit fly
AAS38802          415 NEVNH-FEPK-------------KVYVGGSLKQFTNVSK-----WRG-RSVMYFGDHLYSDLVEPSQKEGWKTGVIIKEL 474 Dictyostel...
AAC63660          348 TKVDA-FDPK-------------KVYYHGCLKSFLEITK-----WHG-PEVIYFGDHLFSDLRGPS-KAGWRTAAIIHEL 406 thale cress
P49902            315 GTYTGpLQHG-------------IVYSGGSSDTICDLLG-----AKG-KDILYIGDHIFGDILKSKKRQGWRTFLVIPEL 375 human

2J2C_A            395 AQELHVWTDKSSLFEEL 411 human
AAK93139          450 LLEDGKYPYAAELDSQL 466 fruit fly
AAP68896          430 EQEVKLLSESKSTRKEL 446 Japanese rice
CAE78341          327 DVEVENNKQAEPINQEI 343 Bdellovibrio bacteriovorus HD100
AAC68740          345 VNELTVWTDSKPLFDEM 361 Caenorhabditis elegans
BAB10319          466 EEEYMALIGSRGHREEL 482 thale cress
BAB14763          383 EREIRII-NTEQYMHSL 398 human
FlyBase:CG1814-PC 344 AHEIKTL-NRKDFKMSA 359 fruit fly
AAS38802          475 EVEVGIQ-NSPKYREQL 490 Dictyostelium discoideum
AAC63660          407 EREIQIQ-NDDSYRFEQ 422 thale cress
P49902            376 AQELHVWTDKSSLFEEL 392 human
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