Conserved Protein Domain Family
tpt

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TIGR00817: tpt 
Tpt phosphate/phosphoenolpyruvate translocator
The 6-8 TMS Triose-phosphate Transporter (TPT) Family (TC 2.A.7.9)Functionally characterized members of the TPT family are derived from the inner envelope membranes of chloroplasts and nongreen plastids of plants. However,homologues are also present in yeast. Saccharomyces cerevisiae has three functionally uncharacterized TPT paralogues encoded within its genome. Under normal physiologicalconditions, chloroplast TPTs mediate a strict antiport of substrates, frequently exchanging an organic three carbon compound phosphate ester for inorganic phosphate (Pi).Normally, a triose-phosphate, 3-phosphoglycerate, or another phosphorylated C3 compound made in the chloroplast during photosynthesis, exits the organelle into thecytoplasm of the plant cell in exchange for Pi. However, experiments with reconstituted translocator in artificial membranes indicate that transport can also occur by achannel-like uniport mechanism with up to 10-fold higher transport rates. Channel opening may be induced by a membrane potential of large magnitude and/or by high substrateconcentrations. Nongreen plastid and chloroplast carriers, such as those from maize endosperm and root membranes, mediate transport of C3 compounds phosphorylated atcarbon atom 2, particularly phosphenolpyruvate, in exchange for Pi. These are the phosphoenolpyruvate:Pi antiporters (PPT). Glucose-6-P has also been shown to be asubstrate of some plastid translocators (GPT). The three types of proteins (TPT, PPT and GPT) are divergent in sequence as well as substrate specificity, but their substratespecificities overlap. [Hypothetical proteins, Conserved]
Statistics
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PSSM-Id: 129898
View PSSM: TIGR00817
Aligned: 10 rows
Threshold Bit Score: 297.793
Threshold Setting Gi: 731957
Created: 8-Oct-2014
Updated: 2-Oct-2020
Structure
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Aligned Rows:
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
P22215   112 ALTELQFLVSAVLCVGFAsIVNlFRLPRLKHTKFSKALNSFPDGILPEYLDgnfRSSILHKFLVPSKLVLMTTFPMGIFQ 191 Saccharomyces cerev...
AAB40647 108 LVLGSLFGLWYIFNIYFN-IYN-KQVLKTFHYPVTITLAQLAVGTILVIFM---WTSNLYKRPKISGAQLAAILPLAVVH 182 common tobacco
P52178   100 LELGLLFAMWYLFNIYFN-IYN-KQVLKALHAPMTVTLVQFAVGSVLITFM---WALNLYKRPKISAAQLAAILPLAVVH 174 Brassica oleracea v...
AAB40650  94 LQLGSLFGLWYLFNIYFN-IYN-KQVLKVLPYPINITTVQFAVGSAIALFM---WITGILKRPKISGAQLFAILPLAIVH 168 Zea mays
AAC28500  95 LKIGIYFATWWALNVVFN-IYN-KKVLNAFPYPWLTSTLSLACGSLMMLVS---WATRIADAPKTDLEFWKTLFPVAVAH 169 thale cress
AAC83815 106 LVTGFFFFMWYFLNVIFN-ILN-KKIYNYFPYPYFVSVIHLFVGVVYCLIS---WSVGLPKRAPIDSNLLKVLIPVAVCH 180 thale cress
P11869   100 LVTGSFFFMWYFLNVIFN-ILN-KKIYNYFPYPYFVSVIHLFVGVVYCLAS---WSVGLPKRAPMDSKLLKLLIPVAVCH 174 spinach
P29463   110 LTTGFFFFMWYFLNVIFN-ILN-KKIYNYFPYPYFVSVIHLAVGVVYCLVS---WGVGLPKRAPIDSTQLKLLTPVAFCH 184 potato
CAA38144 111 ALTELQFLVSAVLCVGFAsIVNlFRLPRLKHTKFSKALNSFPDGILPEYLDgnfRSSILHKFLVPSKLVLMTTFPMGIFQ 190 baker's yeast
P39542   112 LQTVLPLGLFQFVGKYFG--HTaTSLVPVSTVASIKTLSPMFILLLQKILK----ISTLKITLTLIFSLCTLVLGVWIIV 185 Saccharomyces cerev...
P22215   192 FIGHITSHKAVSMIPVSLVHSVKALSPIITVGYYKFFEHrYYNsMTYY--TLLLLIFGVMttcwsthgskRASDNKSGSS 269 Saccharomyces cerev...
AAB40647 183 TLGNLFTNMSLGKVSVSFTHTIKAMEPFFSVVLSAMFLG-EFP-TLWVisSLVPIVGGVG----------LASLTEASFN 250 common tobacco
P52178   175 TLGNLFTNMSLGKVSVSFTHTIKAMEPFFSVVLSAMFLG-EVP-TPWVigSIIPIVGGVA----------LASVTEVSFN 242 Brassica oleracea v...
AAB40650 169 TMGNLFTNMSLGKVAVSFTHTIKAMEPFFSVLLSAIFLG-ELP-TPWVvlSLLPIVGGVA----------LASLTEASFN 236 Zea mays
AAC28500 170 TIGHVAATVSMSKVAVSFTHIIKSGEPAFSVLVSRFFMGeTFPlPVYL--SLLPIIGGCA----------LAAITELNFN 237 thale cress
AAC83815 181 ALGHVTSNVSFAAVAVSFTHTIKALEPFFNAAASQFIMGqSIPiTLWL--SLAPVVLGVA----------MASLTELSFN 248 thale cress
P11869   175 AIGHVTSNVSFAAVAVSFTHTIKALEPFFNAAASQFVLGqSIPiTLWL--SLAPVVIGVS----------MASLTELSFN 242 spinach
P29463   185 ALGHVTSNVSFAAVRVSFTHTVKALEPFFNAAASQFILGqQIPlALWL--SLAPVVLGVS----------MASLTELSFN 252 potato
CAA38144 191 FIGHITSHKAVSMIPVSLVHSVKALSPIITVGYYKFFEHrYYNsMTYY--TLLLLIFGVMttcwsthgskRASDNKSGSS 268 baker's yeast
P39542   186 QEDNRSPASSNELREFSKYGVICAMISMFIFVLQNIYGKtVFT---------YR----------------SQTDESQSNS 240 Saccharomyces cerev...
P22215   270 LIGLLFAFISMIIFVAQNIFAKNILTIRrkvgilpssstddvtskegqpSLDKTRFSPLQVdkITILFYCSCIGFSLTLL 349 Saccharomyces cerev...
AAB40647 251 WAGFWSAMACNLTNQSRNVLSKKFMVRKe-------------------eSLDNITLFSIIT--IMSFILLAPFAFFMEGV 309 common tobacco
P52178   243 WAGFLSAMASNLTNQSRNVLSKKVMVKKd-------------------dSLDNITLFSIIT--LMSLFLMAPVTFFSEGI 301 Brassica oleracea v...
AAB40650 237 WAGFWSAMASNVTFQSRNVLSKKLMVKKe-------------------eSLDNINLFSIIT--VMSFFLLAPVTLLTEGV 295 Zea mays
AAC28500 238 ITGFMGAMISNLAFVFRNIFSKKGMKGK---------------------SVSGMNYYACLS--MMSLVILTPFSIAVEGP 294 thale cress
AAC83815 249 WLGFISAMISNISFTYRSIFSKKAMT-----------------------DMDSTNVYAYIS--IIALFVCIPPAIIVEGP 303 thale cress
P11869   243 WLGFISAMISNVSFTYRSLYSKKAMT-----------------------DMDSTNIYAYIS--IIALFVCLPPAIIVEGP 297 spinach
P29463   253 WLGFTSAMISNISFTYRSIYSKKAMT-----------------------DMDSTNVYAYIS--IIALIFCLPPAIFIEGP 307 potato
CAA38144 269 LIGLLFAFISMIIFVAQNIFAKNILTIRrkvgilpssstddvtskegqpSLDKTRFSPLQVdkITILFYSSCIGFSLTLL 348 baker's yeast
P39542   241 GFSRQESPLPLYEKLDEKLVAKKKPK-----------------------SYDKLTLMIYIS---LVGFCLSFGWFITLEF 294 Saccharomyces cerev...
P22215   350 PFLTgELMHGgSVINDLTLETVALVAIHgIAHFFQaMLAFQLIGLLSSINYSVANIMKRIVVISV-ALFWETKLNFFQVF 428 Saccharomyces cerev...
AAB40647 310 KFTP-AYLEA-SGLNVNQIYTRSLLAAL-CFHAYQ-QVSYMILERVSPVTHSVGNCVKRVVVIVTsVLFFRTPVSPINTI 385 common tobacco
P52178   302 KFTP-SYIQS-AGVNVQQIYTKSLIAAL-CFHAYQ-QVSYMILARVSPVTHSVGNCVKRVVVIVSsVIFFKTPVSPVNAF 377 Brassica oleracea v...
AAB40650 296 KVSP-AVLQS-AGLNLKQIYTRSLIAAC-CFHAYQ-QVSYMILARVSPVTHSVGNCVKRVVVIVTsVLFFRTPVSPINSL 371 Zea mays
AAC28500 295 QMWAaGWQNAvSQVGPNFVW--WVVAQSvFYHLYN-QVSYMSLDQISPLTFSIGNTMKRISVIVAsIIIFHTPIQPVNAL 371 thale cress
AAC83815 304 KLLNhGFADAiAKVGMTKFISDLFWVGM-FYHLYN-QLATNTLERVAPLTHAVGNVLKRVFVIGFsIVIFGNKISTQTGI 381 thale cress
P11869   298 QLMKhGFNDAiAKVGLTKFISDLFWVGM-FYHLYN-QLATNTLERVAPLTHAVGNVLKRVFVIGFsIIAFGNKISTQTAI 375 spinach
P29463   308 QLLQhGFNDAiAKVGLTKFVTDLFWVGM-FYHLYN-QVATNTLERVAPLTHAVGNVLKRVFVIGFsIVIFGNKISTQTGI 385 potato
CAA38144 349 PFLTgELMHGgSVINDLTLETVALVAIHgIAHFFQaMLAFQLIGLLSSINYSVANIMKRIVVISV-ALFWETKLNFFQVF 427 baker's yeast
P39542   295 PVLFrYFFQInSSSTVIKAFPVSLFLLNgTFHFIQaMITFHLLGEVSTLTYSIANLMKRFAIIAVsWVFIGRRITWLQVF 374 Saccharomyces cerev...
P22215   429 GVILTIAGLYGYDKWGLS----KKDGR 451 Saccharomyces cerevisiae S288c
AAB40647 386 GTGVALAGVFLYSRVKGI----KPKPK 408 common tobacco
P52178   378 GTGIALAGVFLYSRVKRI----KPKPK 400 Brassica oleracea var. botrytis
AAB40650 372 GTGIALAGVFLYSQLKRL----KPKPK 394 Zea mays
AAC28500 372 GAAIAIFGTFLYSQVNKQtyscLPTSS 398 thale cress
AAC83815 382 GTGIAIAGVAMYSIIKAK----IEEEK 404 thale cress
P11869   376 GTSIAIAGVALYSLIKAK----MEEEK 398 spinach
P29463   386 GTCIAIAGVAIYSFIKAK----MEEEK 408 potato
CAA38144 428 GVILTIAGLYGYDKWGLS----KKDGR 450 baker's yeast
P39542   375 GLVLNTLGLFLYERCTSQs---KIKAK 398 Saccharomyces cerevisiae S288c
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