5M1U


Conserved Protein Domain Family
CsgF

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pfam10614: CsgF 
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Type VIII secretion system (T8SS), CsgF protein
The extracellular nucleation-precipitation (ENP) pathway or Type VIII secretion system (T8SS) in Gram-negative (diderm) bacteria is responsible for the secretion and assembly of prepilins for fimbiae biogenesis, the prototypical curli. Besides the T2SS that can be involved in the assembly of prototypical Type 4 pilus, the T4SS that can be involved in the biogenesis of the prototypical pilus T, the T3SS involved in the assembly of the injectisome and the T7SS involved in the formation of the prototypical Type 1 pilus, the T8SS differs in that fibre-growth occurs extracellularly. The curli, also called thin aggregative fimbriae (Tafi), are the only fimbriae dependent on the T8SS. Tafi were first identified in Salmonella spp and the controlling operon termed agf; however subsequent isolation of the homologous operon in E coli led to its being called csg. In the absence of extracellular polysaccharides Tafi appear curled, although when expressed with such polysaccharides their morphology appears as a tangled amorphous matrix. CsgF is one of three putative curli assembly factors appearing to act as a nucleator protein. Unlike eukaryotic amyloid formation, curli biogenesis is a productive pathway requiring a specific assembly machinery.
Statistics
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PSSM-Id: 402310
View PSSM: pfam10614
Aligned: 9 rows
Threshold Bit Score: 104.025
Threshold Setting Gi: 1272616090
Created: 11-May-2020
Updated: 7-Aug-2020
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
5M1U_A           2 A----GTMTFQFRNPNF-GGNPNNGAFLLNSAQAQNSYKDPSYn---------dDFGIETPSALDNFTQAIQSQILGGLL 67  Escherichia c...
jgi:M446_2414   23 Ae--aSSLVYQPVNPAF-GGPQLNGSWLQAEANAQNIPQAAAQrrqqitgaaasAARGGILTPGQAFAQQLQTQLYSSLA 99  Methylobacter...
Q6NAU5          19 Ad--aGSLVYTPTNPAF-GGSPLNGSWQMQQATAGNHFNRAAP-----------TSGPQQLTQSQIFAQQLQSQLYASLA 84  Rhodopseudomo...
WP_038561780    19 A----QDFVYTPKNPAF-GGSPYNYSWMLSSAESQNTIEAPEDds-------ydPYSSQSSSSVDDFAESLNRQILSRLS 86  Draconibacter...
jgi:Aeqsu_0406  21 A----QQFSYKPLNPAF-GGDTFNYNWLLSSATSQNSFTAPAN-------------QRDQRSELEKFGDQLNKQLLSQIS 82  Aequorivita s...
WP_044001456    16 GrahaQDFVYEPKNPSFgGGNTFNYSWLLSSAQSQNKIEDPSA-----------SRNNQNTDPLSEFQNNLNRQILSLLT 84  Hymenobacter ...
WP_013909708    21 Sk--aTELIFQFTNPAF-GGNPLYGSYYLQSAQMQNIFKEKKE------------SWFKSKSLIERFTDSFTSQLLYRMS 85  Thermodesulfo...
CCG98076        19 Ak--aQAFVYHPYNPAF-GGNTFNYQWMLSSAQAQDKTKDPAQrss------ssLTNRTSTSSLDDFSTSLQRQILNRLS 89  Fibrella aest...
jgi:Desaf_1459  19 StasaTELVFSFTNPSF-GGDPMIGNFLLNKADSQKRFAEDEY----------------EEDPLSDFEDGLNRRVLGLIA 81  Desulfocurvib...
5M1U_A          68 SNI--------NTGKPG--RMVTNDYIVDIANRDGQLQLNVTDRKTgqtSTIQVS 112 Escherichia coli K-12
jgi:M446_2414  100 NQItrai-fgeNAQPNG--QFSFQGTTISYTRVGSNVSITINDGSTv--TTVTVP 149 Methylobacterium sp. 4-46
Q6NAU5          85 NQVtqai-fgaNAQQSG--TFSFQGTTISFAKVDGQTNITINDGSTv--TQISLP 134 Rhodopseudomonas palustris
WP_038561780    87 RELvtrq-fgeDALEEG--SYVLGDYQIDIGDSDSGINITIVDNVTgstTTVEVP 138 Draconibacterium orientale
jgi:Aeqsu_0406  83 RGLlqqqfggiDFSQEG--TYTFGSLNIEIFQSDEGLVINILDISTgeeTQVIIP 135 Aequorivita sublithincola DSM 14238
WP_044001456    85 TRFvnsqfgngGEIREG--SYNIGSYQIQVTPSGSGVSILITDTTTggqTTVTIP 137 Hymenobacter swuensis
WP_013909708    86 DYIldqlfgedNTLPESpmTYTIGNFRIEYNPSGDYYTFTITDLSTgesSTIQVP 140 Thermodesulfobacterium geofontis
CCG98076        90 QKVignq-fseDTLKEG--TYQFGDFQVTITNATDGVLIRIVDGKGg-eTTVTIP 140 Fibrella aestuarina BUZ 2
jgi:Desaf_1459  82 DKIvqdafgdeGNLSNG--TYTIGSYVISIDNNGKVIKVAVTDSLSgdsTVVEVP 134 Desulfocurvibacter africanus subsp. af...
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