1Z7L,4II2


Conserved Protein Domain Family
UBA_e1_thiolCys

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pfam10585: UBA_e1_thiolCys 
Ubiquitin-activating enzyme active site
Ubiquitin-activating enzyme (E1 enzyme) activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thiolester and free AMP. Later the ubiquitin moiety is transferred to a cysteine residue on one of the many forms of ubiquitin-conjugating enzymes (E2). This domain carries the last of five conserved cysteines that is part of the active site of the enzyme, responsible for ubiquitin thiolester complex formation, the active site being represented by the sequence motif PICTLKNFP.
Statistics
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PSSM-Id: 402288
View PSSM: pfam10585
Aligned: 216 rows
Threshold Bit Score: 66.8592
Threshold Setting Gi: 599385205
Created: 21-May-2020
Updated: 7-Aug-2020
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
1Z7L_A         23 PNAIEHTLQWAR-DEFEGLFKQPAENVNQYLTDs-KFVERTLRLAGTQP--LEV----------LEAVQR---SL----- 80  
CBJ32384      658 PSLIEHCIEWAR-AQFEDLFVGPFAEAKKFCEDkeAYLKQVREATLDCDnrGKAasatakaledLAKLRT---TL----- 728  Ectocarpus si...
XP_002289358  607 PYLPKHCIEFAKqAYFADYFEFGPEVYESFRKDpmSFFEQLDTMEPGDQ----------------SRSLR---MIkafid 667  Thalassiosira...
XP_004990513  619 PHLIDHCIEWAR-AKFTDLFVSPASQLQQFLEDpeGFISGLETKIEQ-HvgGERig-------aLERGVD---TLkaikd 686  Salpingoeca r...
CAM43514      657 PYIYDHCIEWAR-AQFDDMFVSPMQTAQQIIEDpaAFTQRIYHEVASGSsaGERrs-------lIEKNMG---PLkllkr 725  Leishmania br...
Q38DE8        716 PYTSDHCTEWAR-AQFDDLFVSPMQTVRQLLENpaAFTERIKNEVNNAQsaGERls-------lVEKNLG---ILqgiqk 784  Trypanosoma b...
EPR77755      578 PHLIDHTIEWAL-SEFNAQFNKTILSIKEYLSKneDNVDVLSDEIGSD----------------IQEVIK---------- 630  Spraguea loph...
EHY66166      588 PYLPVHCVEWAL-ADFKTLFFERIIEAKRSIse-----------------------------agVDALSEaacDL----- 632  Nematocida sp...
KAE9042437    642 PSLIEHCIEWSR-AQFEDLFVVPSAEAKKFVEDraAYLDQVKKATLENPnpKLVsa-------aIVQELE---RLrslr- 709  Phytophthora ...
KAE9356203    642 PSLIEHCIEWSR-AQFEDLFVVPSTEAKKFVEDraAYLDQVKKATLENPnpKLVsa-------aIVQELE---RLrslr- 709  Phytophthora ...
1Z7L_A         81 ---VLQRPQTWGDCVTWACHHWHTQYCNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLT-FDVNn----TLHLDYVMA 152 
CBJ32384      729 ---AFADGATFESCIQEACGRFYALFRDKVLQLTHNFPEDHVLESGEKFWTGAKRFPRSVD-LEMDs----EQHAAFVLA 800  Ectocarpus si...
XP_002289358  668 lqnEAGGNIDFKGCIRIAFNRMMKDFRTSILDLCYS-ADEMEKSSGKKFWTGTKRRPRAID-WTDPi----PLLMEYLYS 741  Thalassiosira...
XP_004990513  687 laaQLQEKPTMETCVSLAWRDFHAFFRDVILDLIATFPADAKTKSGEPFWSGHKIFPEALV-FDPQn----PLHKEFLIA 761  Salpingoeca r...
CAM43514      726 tltILADGPTMDKCAALGWEQLFKMFRDRILDLQAAFPRGAKRKNGEDFWSGHRKYPSALE-TSTAgisknLDAKNFLVA 804  Leishmania br...
Q38DE8        785 tvtTLSAGVSMEKCVQCAWETMFHLFRDRILDLQRSFPKDAKKKNGEKFWSGHRKYPTPLE-VNIKalssdPDVVEFLIS 863  Trypanosoma b...
EPR77755      631 -----KAPFTKNECIKAGVALFVKLFHTSIRNLISTFPPDHLTEEGTPFWEAPRVAPSPIS-FEYTd----DLHLTFVES 700  Spraguea loph...
EHY66166      633 ---INNIPRSPKEAAGYAVRLFVERFIVGPMKLCESFPRDHITEEGTPFWVPPKKMPRAET-LSLTd----PWHMGYIRS 704  Nematocida sp...
KAE9042437    710 ttlqTAKDITFEKCVTLAFELMTSRFRDRILQLIHNFPEDHLTNSGEKFWSGAKRFPQAVDkFDPEn----PLHLNFVRA 785  Phytophthora ...
KAE9356203    710 ttlqTAKDITFEKCVTLAFELMTSRFRDRILQLIHNFPEDHLTNSGEKFWSGAKRFPQAVDkFDPEn----PLHLNFVRA 785  Phytophthora ...
1Z7L_A        153 AANLFAQTYGLt---------------gS---QDRAAVASLLQ--SvQVPE-FTPKSGVKIH----VSDQELQSANAs-- 205 
CBJ32384      801 TANLLAAGCGLspqee-------gllplEhpqRNTEAVRRVAS--AmDVPM-WENTGEKTDLsegnEPKPGDEKTE---- 866  Ectocarpus si...
XP_002289358  742 TANLYASVWKVegv------------rdR------DEFQAIVDelKlEQPQwEASGEKVDLSegdnEESGSGDVAED--- 800  Thalassiosira...
XP_004990513  762 AANLYACVFKVhptkypseenklhtkrwMaeyRDESWLLSTVG--GrDPPP-YVR-HKVGDLdddsKAAATTDGSGGsdd 837  Salpingoeca r...
CAM43514      805 TINLYACMFGVhppkhear-fnyeksrwMqeyRTDEWIQAEVS--KlTIPA-YVA-GSVDNLdddlAADVQEG--KQ--- 874  Leishmania br...
Q38DE8        864 AANLFACMYGIhpqkhepr-lndpkkrwMqqyRTLDWLNGVMK--NcTVPE-YKP-GSVEGLdddlLQSMEKQ--EV--- 933  Trypanosoma b...
EPR77755      701 AANLYAQSFNV---------------------RDM-----------------NTSTDSLCTAveniKTEDNIIVTINkdd 742  Spraguea loph...
EHY66166      705 TYDLVLRTFSVegslsf-----eaalleY-----------------------FQGKNTSSSAeegnTPDISQIKNEDvs- 755  Nematocida sp...
KAE9042437    786 TANILAVAYGIqpppeq-----klvpadSk-wRDSTTYEELGS--KyVPPT-WKPSNEKIAAdsdeIKRLEQE--KI--- 851  Phytophthora ...
KAE9356203    786 TANILAVAYGIqpppeq-----klvpadSk-wRDSTTYEELGS--KyVPPT-WKPSNEKIAAdsdeIKRLEQE--KI--- 851  Phytophthora ...
1Z7L_A        206 -----------V-------D-------DSRLEELKATLPSpdklPGf---------kMYPIDFEKDDdsNFHMDFIVAAS 251 
CBJ32384      867 -----------Ep-----eDpmdlegaSTELSKLLVELSAvnvsKFr----------FEPADFEKDQdlNFHIDFISATS 920  Ectocarpus si...
XP_002289358  801 -----------D-------------------EELKGELYKidssKLq---------pAQPQEFEKDDdlNFHIDFLTAST 841  Thalassiosira...
XP_004990513  838 dagd-gdddgaAmdeaedeDp------QAAFDALKGEVLTiaknVGsa--------tVEPLDFEKDDddNFHIDFIAAAA 902  Salpingoeca r...
CAM43514      875 -----------Tst----eEa------EAELHGLLADVAAlaskCKgs--------kAAALEFEKDDddNFQIDFVAAAS 925  Leishmania br...
Q38DE8        934 -----------Skd----eTt-----kEQTLNNLLSSVVAlaqkCHnm--------nTVPLDFEKDDddNFHIDFVAATS 985  Trypanosoma b...
EPR77755      743 vvkylneevyfDnyetidfDd---------lENSKKKLKPitvpEDqkii---dpdlLTIIEFEKDDdtNFHVNFIYAAA 810  Spraguea loph...
EHY66166      756 ---------rvV-------------------DKLRTEMMGradpARdtsgvdlssikLEEEEFEKDSevNGHVEYVACAS 807  Nematocida sp...
KAE9042437    852 -----------Kss----nDs-----dKNELIQLLHELETf--dLSgl--------sFEPADFEKDQdmNFHIDFIYAAS 901  Phytophthora ...
KAE9356203    852 -----------Kss----nDs-----dKNELIQLLHELETf--dLSgl--------sFEPADFEKDQdmNFHIDFIYAAS 901  Phytophthora ...
1Z7L_A        252 NLRAENYDISPADRHKSK 269 
CBJ32384      921 NMRAWNYRIKEASRHKIK 938  Ectocarpus siliculosus
XP_002289358  842 NLRSWNYDIKASARHTVK 859  Thalassiosira pseudonana CCMP1335
XP_004990513  903 NLRASNYRIPTATRHKCK 920  Salpingoeca rosetta
CAM43514      926 NLRAENYGIPTQDRMKVK 943  Leishmania braziliensis MHOM/BR/75/M2904
Q38DE8        986 NLRARNYDIPTQDRFKVK 1003 Trypanosoma brucei
EPR77755      811 NLRAMNYKIKTTDRLTVK 828  Spraguea lophii 42_110
EHY66166      808 NIRARMYGIDTLDRLEVK 825  Nematocida sp. 1 ERTm2
KAE9042437    902 NLRAFNYRIHDASRHKCK 919  Phytophthora rubi
KAE9356203    902 NLRAFNYRIHDASRHKCK 919  Phytophthora fragariae
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