Conserved Protein Domain Family
Atg14

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pfam10186: Atg14 
Vacuolar sorting 38 and autophagy-related subunit 14
The Atg14 or Apg14 proteins are hydrophilic proteins with a predicted molecular mass of 40.5 kDa, and have a coiled-coil motif at the N-terminus region. Yeast cells with mutant Atg14 are defective not only in autophagy but also in sorting of carboxypeptidase Y (CPY), a vacuolar-soluble hydrolase, to the vacuole. Subcellular fractionation indicate that Apg14p and Apg6p are peripherally associated with a membrane structure(s). Apg14p was co-immunoprecipitated with Apg6p, suggesting that they form a stable protein complex. These results imply that Apg6/Vps30p has two distinct functions: in the autophagic process and in the vacuolar protein sorting pathway. Apg14p may be a component specifically required for the function of Apg6/Vps30p through the autophagic pathway. There are 17 auto-phagosomal component proteins which are categorized into six functional units, one of which is the AS-PI3K complex (Vps30/Atg6 and Atg14). The AS-PI3K complex and the Atg2-Atg18 complex are essential for nucleation, and the specific function of the AS-PI3K apparently is to produce phosphatidylinositol 3-phosphate (PtdIns(3)P) at the pre-autophagosomal structure (PAS). The localization of this complex at the PAS is controlled by Atg14. Autophagy mediates the cellular response to nutrient deprivation, protein aggregation, and pathogen invasion in humans, and malfunction of autophagy has been implicated in multiple human diseases including cancer. This effect seems to be mediated through direct interaction of the human Atg14 with Beclin 1 in the human phosphatidylinositol 3-kinase class III complex.
Statistics
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PSSM-Id: 370867
View PSSM: pfam10186
Aligned: 28 rows
Threshold Bit Score: 136.032
Threshold Setting Gi: 74582122
Created: 21-May-2020
Updated: 7-Aug-2020
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Q4WCL3         3 CPICSRVSssrLRFYCPTC---ACN--------------QIYTLRIDNARALLEKETLGRQVEKALLHQTsptlsyhrfe 65  Aspergillus fum...
XP_002496378   3 CSICHKSG---RPMYCAHC---MSSsp-----------hMLLKLKLDLLILRETNGHLKSRVEDILEYGLgqi----agk 61  Zygosaccharomyc...
Q6FX35         5 CPICETQS---HVFYCAHC---INSsp-----------dLLIRLKFDLYILGKINNALRNKVDQYLEEVDdeln--tnir 65  [Candida] glabr...
Q5KCM1         9 CNCCQVNQ---KALYCPAC---LRE--------------GITLHKELLQNLQARLSTIRQHSQKLLTGTE---------- 58  Cryptococcus ne...
EDR01346       3 CKICEFKQ---RQFFCQTC---LKT--------------HLRDFQHQTQHFAADRDEQVLKASKALETIQ---------- 52  Laccaria bicolo...
Q17M47        35 CPLCNYSK---RHFHCKSC---IRKgdfihs--thhileRFSEKQLRHMNLKSAHATLEGKCVKLLERRR---------- 96  yellow fever mo...
Q6ZNE5        43 CPLCNTTR---RRLTCAKC---VQSgdfvyf--dgrdreRFIDKKERLSRLKSKQEEFQKEVLKAMEGKW---------- 104 human
EDO36555       3 CPLCLRRS---KYLTCCSC---LRSgsvtht---gkrpeSYCEKVKRLADLKHVKEAFQKRVTEEIANRQ---------- 63  starlet sea ane...
Q54QH5         7 CCSCNNRFn-fQESYCEPClqrIKNdq-----------sRVFELIQSIFDKKKANLIAKNKILESISQPK---------- 64  Dictyostelium d...
O42862         7 CQLCETR----RPSICESC---LKKqlydfyhkrdefsrDIESELEKVAKLKSESFSLKGKITQKEELTY---------- 69  Schizosaccharom...
Q4WCL3        66 ECSTQSPDKVPSPQDVLQIITAKAESSIR----------RKELALQIQQLKSEIKDRQLGISQRRLTLA----------- 124 Aspergillus fum...
XP_002496378  62 EPKSASTNVEGDILGRRLRKLDSVILRRK----------NNRVKYRISQLNRRIYEKRHRIEALRSKL------------ 119 Zygosaccharomyc...
Q6FX35        66 NGEQLESNIGVQILKERLGKVQVLRKERQ----------NNKIKHKISQQDRRIKEKSRYIAELRSLI------------ 123 [Candida] glabr...
Q5KCM1        59 --NETKDKADRGLNAWRELRSDVASSEAR----------CHQIKMTISQRQAAISACRKRIAENNIR------------- 113 Cryptococcus ne...
EDR01346      53 --------------AARLFRAHVADCQSR----------LEEVMAGLAQLRKENDKKRDRLRALRESLA----------- 97  Laccaria bicolo...
Q17M47        97 LSCQLRAEIKQRTENIDNIRKLMALKREN----------VGRLQALRKELYESNRLLRMKLPRCDDQVKrissyvlskle 166 yellow fever mo...
Q6ZNE5       105 ITDQLRWKIMSCKMRIEQLKQTICKGNEEmeknsegllkTKEKNQKLYSRAQRHQEKKEKIQRHNRKLGdl--------- 175 human
EDO36555      64 AVQQLNDAVILKREKISCIQSVIRLIQQH----------TSQDQQSLDSIRKSNAVKKVKLKSNADEIKtllevkkkhla 133 starlet sea ane...
Q54QH5        65 --NQLQEQIDIKKTYLESKKIQIEILKNK----------IQTLKTTVIKDNGLIDVKKKYLSTRRSSLK----------- 121 Dictyostelium d...
O42862        70 -------RLQNLAASLNEKKSLSCDLHSR----------KQLIEDDLSNRKKSINIASQKLAGLSHSTSdyfsk----ey 128 Schizosaccharom...
Q4WCL3       125 ---RRRSDAESSKFQLESREVALICGVQNTIK----------------RTERL------------WHSLHSKTVEA---- 169 Aspergillus fum...
XP_002496378 120 ---QLPSKIGIHEKELKAKRESQELTIS--------------------EGKQQ------------LTQIRKVLLKS---- 160 Zygosaccharomyc...
Q6FX35       124 -----------NGPPKNQNRFTDPQTILKAQK----------------QLQDK------------LEIAKRLSIQT---- 160 [Candida] glabr...
Q5KCM1       114 ---DRRES-----LSMLKSRPSPVSSLRDSIK----------------QVHSQ------------QQVITSRIIHA---- 153 Cryptococcus ne...
EDR01346      98 ---TRRRS--LSAAKLQPARHSPAPGLSHLTQ----------------RETQE------------LNALSMTIARA---- 140 Laccaria bicolo...
Q17M47       167 eseRRRASINELQDEIKQFRRDQIGKLIRYIF----------------PISRA------------ISNRSGFSSS----- 213 yellow fever mo...
Q6ZNE5       176 -veKKTIDLRSHYERLANLRRSHILELTSVIF----------------PIEEV------------KTGVRDPADVSse-s 225 human
EDO36555     134 tlsKRMRDLKSLQNELGQVRQRSMALVQKYLF----------------PIQAIpvypvmgtppppQHTPESLDLSRelsf 197 starlet sea ane...
Q54QH5       122 --------KNKISFEAVNNSKSGFSSLTDEIQ----------------KKKDQ------------LTAENTSLQIV---- 161 Dictyostelium d...
O42862       129 ltaYRRSE--LLQNKLHEYQKKLITRVLDMYQlhwqrdlvlntqgctqKSAQM------------GSEFNPDSIDSsl-a 193 Schizosaccharom...
Q4WCL3       170 -RIFLCREVANLYSLRQ----------WMKQDGSEMKETYVIGG--VPIVDLRDLNGksqncpllldm--------paps 228 Aspergillus fum...
XP_002496378 161 -QLSKIRSLVEWFVIRK---------------RESYEFPYTLAF--QPIVSLRNVYK----------------------- 199 Zygosaccharomyc...
Q6FX35       161 -RSEKLAMLNKWYSIRK---------------RDSHDIPFTISY--QPVISLKNFNR----------------------- 199 [Candida] glabr...
Q5KCM1       154 -RRVLVREAVNVFGVRQ-----------------RPSGDWEIAG--IPLPDPETFRV----------------------- 190 Cryptococcus ne...
EDR01346     141 -RSGLVQELVEVFNVVEvg------grPPIGGKAGTKGEWTIGD--LILPVPGDIRR----------------------- 188 Laccaria bicolo...
Q17M47       214 -DGESKHTISEISDATRtayv---rgkWVLQDS-FGEIQHIIVA--PALPGNGNYSAyndwatvnpatdvsstgrsedgv 286 yellow fever mo...
Q6ZNE5       226 dSAMTSSTVSKLAEARRttyl---sgrWVCDDH-NGDTSISITGpwISLPNNGDYSAyyswveekkt----------tqg 291 human
EDO36555     198 eDQADASVESALAEATQtsyv---qgkWV---NETATLELSIID--SSLPWSGDYSAyrhwvkahkng-------skgpd 262 starlet sea ane...
Q54QH5       162 -KRSKIEQLTNSMTRLQ--------------LNPTTPDHFFLFN--MVLNDQQLLK------------------------ 200 Dictyostelium d...
O42862       194 yRLSKLSMGNSKSDLSHsdnetghfysNFFSQVMELNASVTISG--IPVCIRSKEKM----------------------- 248 Schizosaccharom...
Q4WCL3       229 inaqigATAVQISTSFSNIAHLLVLVSHYLSLRLPAEITLPHKDHPVPTIHAPIASYISRESILTAtstq-phnLASSTT 307 Aspergillus fum...
XP_002496378 200 ------LPSSVVWGSISTMSRYLKLFAEILFFTLPHNELVAPNAP----------------------------------- 238 Zygosaccharomyc...
Q6FX35       200 ------LPLPLIEGSLRKLIQYMNLLEHIYCIKYPSAMFVHEDEM----------------------------------- 238 [Candida] glabr...
Q5KCM1       191 ------HPSSSINAALSHVIHLVSLITTYLSISLPFVPMPPPPFYHPHVGRPLMKANLPFVSTTKWrekhvlwmSSTASV 264 Cryptococcus ne...
EDR01346     189 ------YPPDHINAVITHTIHFLSLLTFYLGVKLPFEFVWTGGKLGVGQPWIGAIMGSESGGWARWstk--hplHLSSSP 260 Laccaria bicolo...
Q17M47       287 rnvasrNPAHTIAAALTYTAQLVHIVCFYLDIRAPYKVSYSDFCT----------------------------------- 331 yellow fever mo...
Q6ZNE5       292 pdmeqsNPAYTISAALCYATQLVNILSHILDVNLPKKLCNSEFCG----------------------------------- 336 human
EDO36555     263 ddvglrNPAYTISSALTHAAQLLELLASLLDVNLPSKLSYSEFCQ----------------------------------- 307 starlet sea ane...
Q54QH5       201 ------FPKDVIFTTLGYISKIVVLISGILGITLPYHLLSKGSKSTI--------------------------------- 241 Dictyostelium d...
O42862       249 ------FLNPDCALTLSFICIFLA---QYTSIPLPCPLQLPSPDQ----------------------------------- 284 Schizosaccharom...
Q4WCL3       308 HPLGFQSNHYQPRPLTIDRSLPKLAR--EDPESYVLFLEGVTLLAWNVSWLCRTQGLNI--------ASDSWEEI----- 372 Aspergillus fum...
XP_002496378 239 ----------------ELELEVSAKTadESSSDENTIVDHLTKIFINIIQISRHLNLISkd----piDLAWMLDQ----- 293 Zygosaccharomyc...
Q6FX35       239 --------------------LSLQGHsaKLKRNAPELLQVLIKLIQMILVVMTRSKLIDerk--qniDYAWILDQ----- 291 [Candida] glabr...
Q5KCM1       265 SSKIKPQSTPPISNLLAQPSICSIIA--KSTSKHKQFLVSFALLSFSIAYLAWSQDVPGigirgevdGDDNAGVL----- 337 Cryptococcus ne...
EDR01346     261 NPPQPDETPPTPTAPLSESYIDTTP----PPNTPSSFTTALAMLLYDVSYLAHTQNVDV-----------PLSQA----- 320 Laccaria bicolo...
Q17M47       332 --------------T---------------ALSEAQFNRKVARLNANILYLCYTQGCQLnel----rptHTLENI----- 373 yellow fever mo...
Q6ZNE5       337 --------------E---------------NLSKQKFTRAVKKLNANILYLCFSQHVNLdql----qplHTLRNL----- 378 human
EDO36555     308 --------------N---------------EMGRKEFQSALSRLNTNVLHLCFTQHVDPtll----hphRTLNNL----- 349 starlet sea ane...
Q54QH5       242 ------------HHNMKSKDYPLYYQ---QKTKSREFQKALHLLGENIIFLRYYHGIQTp-------KESNLLFL----- 294 Dictyostelium d...
O42862       285 ------------------------------KPMTSFSSEQMLYIMYNIVWISWNCGIFSfprgitqsQLFELMQYtgfwl 334 Schizosaccharom...
Q4WCL3       373 ----------------------CNIGKNMW----------------QLLVA 385 Aspergillus fumigatus
XP_002496378 294 ----------------------NDLDTLFY----------------NMAMR 306 Zygosaccharomyces rouxii
Q6FX35       292 ----------------------YDIDGLFY----------------HMAMS 304 [Candida] glabrata CBS 138
Q5KCM1       338 ----------------------FDEES-------------------KVTQG 347 Cryptococcus neoformans
EDR01346     321 ----------------------GDVLSNLW----------------SVCCS 333 Laccaria bicolor S238N-H82
Q17M47       374 -------------------------QRLMN----------------STHAD 383 yellow fever mosquito
Q6ZNE5       379 -------------------------MYLVS----------------PSSEH 388 human
EDO36555     350 -------------------------YALIN----------------TTCTG 359 starlet sea anemone
Q54QH5       295 --------------------------KNIY----------------ELIKS 303 Dictyostelium discoideum AX4
O42862       335 vqlrtkpltsqkpdwhykmgmdFDLFHRLYlarlpipinysslrsrSSSKP 385 Schizosaccharomyces pombe 972h-
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