3PUF,3P5J


Conserved Protein Domain Family
RNase_H2_suC

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pfam08615: RNase_H2_suC 
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Ribonuclease H2 non-catalytic subunit (Ylr154p-like)
This entry represents the non-catalytic subunit of RNase H2, which in S. cerevisiae is Ylr154p/Rnh203p. Whereas bacterial and archaeal RNases H2 are active as single polypeptides, the Saccharomyces cerevisiae homolog, Rnh2Ap, when expressed in Escherichia coli, fails to produce an active RNase H2. For RNase H2 activity three proteins are required [Rnh2Ap (Rnh201p), Ydr279p (Rnh202p) and Ylr154p (Rnh203p)]. Deletion of any one of the proteins or mutations in the catalytic site in Rnh2A leads to loss of RNase H2 activity. RNase H2 ia an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. It participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication.
Statistics
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PSSM-Id: 400782
View PSSM: pfam08615
Aligned: 19 rows
Threshold Bit Score: 72.3991
Threshold Setting Gi: 74687096
Created: 11-May-2020
Updated: 7-Aug-2020
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
3PUF_L        31 TLHLLPCEVAVDGPAP-VGRFFTP-------------------------------------AIRQGPEG----------- 61  human
EDK40448      14 TVSLLPLHIQYSGPAN-TKEYFSDtk----------------------------------sTEKVGNEEr---------- 48  Meyerozyma guil...
CDS46262     114 NTNIFPFHIKKNGKIN-SDIFFIPyisednkdvitkytynydyydiypqnveltnknfhanKRKDIDSSrhgnlvkckke 192 Plasmodium berg...
Q8I300       109 DTNIFSFHIKKNGKIN-SDIFFIPynsiddedviteytysynyydiypvnieqvnksfhsnKKRKIIKNknintnnqlli 187 Plasmodium falc...
Q6CE25        43 VCHVLPCHIHYTGPAN-VAEYFTIeggdnkngqqvevtkde----kadenpevakkaeendAAKEQGEEdekefglnnrd 117 Yarrowia lipoly...
XP_002881655  91 qVHQLPCCIRFDGPAD-VSHYFKPqssvrlm-----------------------wravefvDVEIDGVRt---------- 136 Arabidopsis lyr...
XP_001646912  16 LGHLVPCKIRYNGPSDeFTDNFNMdneem---------------------------evlnnDVADESKQv---------- 58  Vanderwaltozyma...
EDO06576       6 NVHVLPCLIKQPGDADiQGGFTDNiisldsvspe-----------------tplgglsisdICGSVSRDvnfgvsgterl 68  Babesia bovis
Q4D5R7        14 VVHSLPFKTTFRGTTDiEENFSRH-------------------------------------IEHTENGG----------- 45  Trypanosoma cruzi
Q4GYA5        23 VIHSLPVKTNFRGTTDvTVNFTQH-------------------------------------TVRGEGGE----------- 54  Trypanosoma brucei
3PUF_L        62 -------------------------------LEVSFRGRCLRGEEVAVPP-GLVGYVMVTEEKKVsmgkp---dplrDSG 106 human
EDK40448      49 -------------------------------EIAYFRGCRLVGHKTNWDG-KYCGYIMNKSETIAh----------aNPA 86  Meyerozyma guil...
CDS46262     193 hid---------------kdeneneksnfqkFLVHFRGRLFIGSNINYSFfNIQTFLATLSN----------------DD 241 Plasmodium berg...
Q8I300       188 kkeldednndinneknenyndiknkrsnfqkLLVHFRGRLFIGCHINYSYfKIKTFLAMIKN---------------NED 252 Plasmodium falc...
Q6CE25       118 s------------------kdpkdksassapLSTHFRGRKLHGVEQNLPE-TYRGYRFTEGTNFFensnpvsqlgdiTQD 178 Yarrowia lipoly...
XP_002881655 137 -------------------------------EEAHFRGRKLQGATISLPS-GYSGFVLGQASNLN------------ANG 172 Arabidopsis lyr...
XP_001646912  59 -------------------------------YVNYIRGRKIIGQDV-LPIfGEGSKAFLTENVIS------------SDA 94  Vanderwaltozyma...
EDO06576      69 scdv------------yssqygsdpflgkerLCTFLRGRCLRGSKLNFDAiGCRMSMVEVCNSTRq-----------RNA 125 Babesia bovis
Q4D5R7        46 -------------------------------LRNRLRGRTLIGREVVLPP-SYVLAVTSFMPRQQhplg-----tgaAGC 88  Trypanosoma cruzi
Q4GYA5        55 -------------------------------LRNALRGRTLMGREVILPS-SYVIACASFTNKPPiaeaggrksggaAAC 102 Trypanosoma brucei
3PUF_L       107 TDDQEEEPLERDFDRF-------IGATANFSRFTLWGLETIP--GPDAKVRGAlTWPSLAAAI 160 human
EDK40448      87 DTEGIANGTAEIKNVKv------YNPIAKFDSMTLYGHDNVI--ESTSQWRMVsEWSEISDAI 141 Meyerozyma guilliermondii ATCC 6260
CDS46262     242 HTTSETQNKLCYVNKK-------IKTYNHIPNATYWKQDEYPd-IFDSNIQKL-QMFFISQSI 295 Plasmodium berghei ANKA
Q8I300       253 IDDTENLPLFNYVNKN-------IQTYNLIENATYWKQDEYPd-YTDQNIQKL-FFFHI---L 303 Plasmodium falciparum 3D7
Q6CE25       179 FDQDGFERNPDDYRNNepiemrsWNAEGSFDHLVIWGHERVPdlASDQWACGVrDWHQVATAM 241 Yarrowia lipolytica
XP_002881655 173 KRKASMPAEEN---QC-------WEVKAKFDNLTYWNHDSLPs-KDDTFLRSF-HWFSIAEAI 223 Arabidopsis lyrata subsp. lyrata
XP_001646912  95 TMEEEFDNTESIN----------AKPIATLNKLINYERDGNEs-RLSSELQKFqEYIELNDII 146 Vanderwaltozyma polyspora DSM 70294
EDO06576     126 SSRSGLTPSYT------------LGELGPVESFTVWDRDSDIs-PLSPCLEGY-KHIAVARAL 174 Babesia bovis
Q4D5R7        89 VTSSAPSLAQVEAVASd------VRITAIEDRFCVWEHDKQP-----ERVDALaHWLELSRTL 140 Trypanosoma cruzi
Q4GYA5       103 FDTTQPSSAQVEAAAGd------MRITAVAESFCIWEHDKKP-----ERSEMVtQWLRLSNDL 154 Trypanosoma brucei
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