Conserved Protein Domain Family
SAE2

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pfam08573: SAE2 
DNA repair protein endonuclease SAE2/CtIP C-terminus
SAE2 is a protein involved in repairing meiotic and mitotic double-strand breaks in DNA. It has been shown to negatively regulate DNA damage checkpoint signalling. SAE2 is homologous to the CtIP proteins in mammals and an homologous protein in plants. Crucial sequence motifs that are highly conserved are the CxxC and the RHR motifs in this C-terminal part of the protein. It is now known to be an endonuclease. In budding yeast, genetic evidence suggests that the SAE2 protein is essential for the processing of hairpin DNA intermediates and meiotic double-strand breaks by Mre11/Rad50 complexes. SAE2 binds DNA and exhibits endonuclease activity on single-stranded DNA independently of Mre11/Rad50 complexes, but hairpin DNA structures are cleaved cooperatively in the presence of Mre11/Rad50 or Mre11/Rad50/Xrs2. Hairpin structures are not processed at the tip by SAE2 but rather at single-stranded DNA regions adjacent to the hairpin. The catalytic activities of SAE2 are important for its biological functions.
Statistics
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PSSM-Id: 400750
View PSSM: pfam08573
Aligned: 68 rows
Threshold Bit Score: 54.7177
Threshold Setting Gi: 74863551
Created: 21-May-2020
Updated: 7-Aug-2020
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
EKG11201     685 AYSEVVRSREARQCLPG--CTKpdcCGRDLRAMVEAGLNPPLPrsfwddsq------ptyaqtfVSDKEAQDHRYLQWFQ 756 Macrophomina ph...
XP_002910185 500 QYDEVVRGREARRQMDAgdCEC---CREYYNAVGPLPPRPPGLlwaspsst------pkrckhrAGKADEEDEEDHETP- 569 Coprinopsis cin...
XP_003026714  22 QFEDVVRGRESRKRLEAtdCEC---CREYYEAVGPLPARPQGPlwrspsrs------prkhrpeCQHHQDDRRQD----- 87  Schizophyllum c...
EMD37051     545 QFDDVVRNKQQRKNLHAddCEC---CRDYYEAIGPLPSRLRPPlwrspest------pskpssrPHKHLGGALSTTALSS 615 Gelatoporia sub...
EED78212      20 QFDEVVRNKGQRRHMHAfdCEC---CREYYEAVGPLPARLQAPlwkspqst------pskrtarSEHLTSVDLDIDADE- 89  Postia placenta...
EPT02363      19 pFDEVVRDKACRKRLRGadCEC---CREYYKAVGKLPPGPQPPlwrsphat------pmkatspGRRHLADASSVPNSPR 89  Fomitopsis pini...
EDR09259     594 QYDEVVRGKEQRRHMNAgdCEC---CRDYYEAVGPLPNRLQQPlwrspssk------pcarhsyENLSSSTSSARRDKGS 664 Laccaria bicolo...
EGN94222      55 dryETVRNKEQRKQLLGddCEC---CRDYYAGVGPLPPRLQQPmwrspspnsipttkplckhrqTDTYATNVGASGSME- 130 Serpula lacryma...
ELU44816     548 QFDSVVRDKNERKKLAAgdCIA---CKEYYEAVGAMPARPRAPlwkspppeg---agpslrphsCKHREGGADLSVSDDV 621 Rhizoctonia sol...
EKM55809       3 KYEEVVRNKRERRRLHGgdCEC---CRDYYEAIGPLPSRLQAPlwrtppss------paknhpsSSYHENNYSG------ 67  Phanerochaete c...
EKG11201     757 G---TSydRDRVHampsaMRDDLVVQAKVKllasQAGKHRHAHQRPRTPPGFWRADFP 811 Macrophomina phaseolina MS6
XP_002910185 570 ------------------TRRQKDIDAHKK----GISRHRHNWERAKTPPDYWNIGFP 605 Coprinopsis cinerea okayama7#130
XP_003026714  88 ------------------DHRDEQVQAHRQ----AISRHRQQWARAKTPPGYWEIGFP 123 Schizophyllum commune H4-8
EMD37051     616 TdsqDEppTTAD------AEQADAITEHRH----QISRHRHQWARAKTPPGYWDIGFP 663 Gelatoporia subvermispora B
EED78212      90 ------------------AEQEQAIAKHRQ----EISRHRQQWERPLTPPGYWEIGFP 125 Postia placenta Mad-698-R
EPT02363      90 KrrvRDedGDEDA-----DEDTAAIAAHVQ----QISRHRQQWERPKTPPGYWEIAFP 138 Fomitopsis pinicola FP-58527 SS1
EDR09259     665 S-----------------SSHEREVDTHKK----AISRHRHTWARANTPPGYWNIGFP 701 Laccaria bicolor S238N-H82
EGN94222     131 ------------------QVQEEEITRHKQ----AISRHRHHWYRPKTPPGYWDIGFP 166 Serpula lacrymans var. lacrymans S7.3
ELU44816     622 D-----------------MEDDVGLAAHKQ----AISRHRHKWVPASTPPDYWNIGFP 658 Rhizoctonia solani AG-1 IA
EKM55809      68 ------------------DEREADIQDHKQ----QVSRHRTRWEAPKTPPGYWNIGFP 103 Phanerochaete carnosa HHB-10118-sp
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