Conserved Protein Domain Family
Cut8

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pfam08559: Cut8 
Cut8, nuclear proteasome tether protein
In Schizosaccharomyces pombe, Cut8 is a nuclear envelope protein that physically interacts with and tethers 26S proteasome in the nucleus resulting in the nuclear accumulation of proteasome. Cut8 comprises three functional domains. An N-terminal lysine-rich segment which binds to the proteasome when ubiquitinated, a central dimerization domain and a C-terminal nine-helix, Structure 3q5w, bundle which shows structural similarity to 14-3-3 phosphoprotein-binding domains. The helical bundle is necessary for liposome and cholesterol binding. Cut8 is a proteasome substrate and the N-terminal segment is polyubiquitinated and functions as a degron tag. Ubiquitination of the amino N-terminal segment is essential for the function of Cut8. Lysine residues in the N-terminal segment of Cut8 are required for physical interaction with proteasome. In fission yeast the function of Cut8 has been demonstrated to be regulated by ubiquitin-conjugating Rhp6/Ubc2/Rad6 and ligating enzymes Ubr1. Cut8 homologs have been identified in Drosophila melanogaster, Anopheles gambiae and Dictyostelium discoideum.
Statistics
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PSSM-Id: 400736
View PSSM: pfam08559
Aligned: 83 rows
Threshold Bit Score: 151.558
Threshold Setting Gi: 74856897
Created: 21-May-2020
Updated: 7-Aug-2020
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Q6BWL0        48 GKKRRYsedqeskvvkptgnrkhytt-----------------pstyykskksrtpkimgqklPINRLVEALDHTSLQRL 110 Debaryomyces ha...
EFX77859     104 cSPRKRlqlnetgetismsppekmr--------------------klssssnrkpikleddpvPLDLVLNGLSRSQLISV 163 common water flea
XP_002432926 119 SSPRKRlllndpkelclsspekltkkvsptkklr-sdkssnslfsgnqnnnnnnnnnmpgpssTFQSALKGLSQEQLIKI 197 human body louse
XP_001944112 114 STPRKRlllndplelsspdkykigs--------------------psckklrtevavelpednCLLSALKSLSPDQLIGI 173 pea aphid
XP_001119908 118 STPRKRlsladnsesqfttpdkkk----------------------klqnlldtnssqkyftgNLLNGLRGLSHNQLVHM 175 honey bee
XP_011138388 113 STPRKRlslidicqsefttpekkr---------------------ktyslmaevntsqtqpidDLMNGLQGLSHEQLVQM 171 Jerdon's jumpin...
EFN66760     109 daARKRlslgdtnqskfptpkskki-------------------lssrmsldaanmlrqrskdNLVNGLRNLSHEQLVQL 169 Florida carpent...
OWR49691     108 STPRKRlllgdnervpltpekidfsdistpqkf---kitspfkdsppikrsrlertfdgefkgPLDTALKGLSPTQLIHM 184 Danaus plexippu...
XP_012547683 112 sTPRKRllmgeteripltpekidfsdistpqkfklnnqecsikntppmkrsrvekvldkeyrgPIDTALKGLSPTQLIHI 191 domestic silkworm
XP_011660675 116 SSPRKRnitqtsdyyshlgsaeesilq---------------esvswlrrakkarkerresalDLVKRLRALSVDQLATV 180 purple sea urchin
Q6BWL0       111 LESLIH--------EHPEISNTIDKISPKPTLTDSIELIKQKFNSIMHHLPYk--CDTESDYSYLRIKTHLNEFLNCLSD 180 Debaryomyces ha...
EFX77859     164 IQNLAT--------INEEVKQKLESLLPEPDLQPMLDELSYLKKNIFKSLPNcrlSSKTDSAAYNKTSVHLSAFKKRLLD 235 common water flea
XP_002432926 198 FENVLM--------KHPHLES------VTPDIKPLEDKLIYLKKNIFKSLPNsrlTSKTDSPAYSRAATHVVAFKKCVLE 263 human body louse
XP_001944112 174 IGQIVI--------DHPSIEKEIRSHFPVADLKPLEERIYYLRRNIYKALPSsrlISKTDPTAYNRVSTHVLAFKKCVVD 245 pea aphid
XP_001119908 176 IMDLVSmqedgllhNNDKIRNILLKKMPMADIQSLIDILNNLKQNIQVSIGFsdlNGLSN--------IHLDAFHKAIID 247 honey bee
XP_011138388 172 IMDMVHmqesgllcKSTTLRNILLKKMPVADIQQLIQKLRVLRQNIYASLIC----SNEDNSAYNRAYIHLNTFEKTLID 247 Jerdon's jumpin...
EFN66760     170 IMELVYaqedgtlcENEKLRYILSKKISDGDIQPFIEKLILLKQNIYANPVFs--PNLNDELAYSCAYMHLDAYQKALID 247 Florida carpent...
OWR49691     185 IKRITH--------KHPEVEKEIRSDMPIPDLTPLEEKLSYLKSNIFKSLPTsrlTSKTDSPAYSRVATHLTAFKKCLVD 256 Danaus plexippu...
XP_012547683 192 IKNITY--------KHPEVEQELRREMPLPDLSPLDEKLSYLKSNIFKSLPTsrlTSKTDSPAYSRVSTHLAAFKKCVID 263 domestic silkworm
XP_011660675 181 IGDLIK--------QHPDLEEDLQDLVPEPDISSCEEEVCELLHNIYRAMPRsrlSSSRGAYSYRQVRLHIIKFKKECIQ 252 purple sea urchin
Q6BWL0       181 FILNFLPPIEtnVFNSLKFLDEVTDLIHTLPNFSNSEFQYTKSMAYEQISNTWLIVLNHkmrpddgtnnnntsntneals 260 Debaryomyces ha...
EFX77859     236 YCKQLVESRQ--WKSVVEFTLNAWNLVKATPIWDNAQHNSVRKQCFKSLSTNCLKALK---------------------- 291 common water flea
XP_002432926 264 QGKTLLESQH--WNSVLEYVLLAWGYVKATPLWDNPPHNASRKQCFKSLAAMAISALK---------------------- 319 human body louse
XP_001944112 246 QGRRLVESNQ--WPIVMDYVFMAWKHVRNTPIWDNPAHNAARRQCFKSLSAQCITALKH--------------------- 302 pea aphid
XP_001119908 248 QGKRLVESQH--WISVMHYVYAAWNITKQLNEKEHQSICNLTHKCFKNLTHFCSQALK---------------------- 303 honey bee
XP_011138388 248 QGRTLQDSQH--WISLVHYALAAWEITKELPEWENQGHHNTTRKCFKSLSQFCAEAIR---------------------- 303 Jerdon's jumpin...
EFN66760     248 QGRILQESQH--WISLMHYVLEAWKITRELPKWENQDC-NITHKCFQSLSQFCAETIR---------------------- 302 Florida carpent...
OWR49691     257 QGKVLVESQH--WESVIKYVFLAWNYVRATPLWDNQPHNAQRKQCFKTLINFCMTALK---------------------- 312 Danaus plexippu...
XP_012547683 264 QGKVLVESQH--WESVLHYVFLSWNYVKSTPVWDNQPHNAHRKQCFKALTNFCLTALK---------------------- 319 domestic silkworm
XP_011660675 253 HGRHFVKCKA--WDAAIRYVLVAWQQVHKLPVWECPAHNRLRTTSYLGLAGVCVEALR---------------------- 308 purple sea urchin
Q6BWL0       261 sssspspvaqcnmenSAQLikIIDELNLQQKLAKHDNMSIGkFKLVVEFVNSEIE 315 Debaryomyces hansenii CBS767
EFX77859     292 ----------------SADgeLEWRHEIKKRLERLRGDSDD-LDMCIAYLDTAdg 329 common water flea
XP_002432926 320 ----------------KGSwcNEELEDIYIRFKKCDSESDD-IQACLKQISSLrk 357 human body louse
XP_001944112 303 ---------------MKDTmnQQSCDNYKNQLKLLVDDSED-IEWCLHFLNire- 340 pea aphid
XP_001119908 304 ----------------KGNftSTVLDMFADRLDAMVEDYED-LKVCLQLINEVKN 341 honey bee
XP_011138388 304 ----------------RGNfeISDLETYIARFKTISEECEE-FKICLQIAKEAKQ 341 Jerdon's jumping ant
EFN66760     303 ----------------RGNfdISTLEIYIESLKIIVEECED-FQICLQIAKEAKH 340 Florida carpenter ant
OWR49691     313 ----------------KGTfdKDYMIDIHDKLQGIESDSED-VQSCLKYVNGQLq 350 Danaus plexippus plexippus
XP_012547683 320 ----------------KGNfeKDLLIDVQDKLQEMICDSDE-LHSCLKIVQGql- 356 domestic silkworm
XP_011660675 309 ----------------KGAfsLQTLQEFRPKIEAMVSSAPE-MAACLQRLDIMte 346 purple sea urchin
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