2EXU


Conserved Protein Domain Family
Spt4

?
pfam06093: Spt4 
Click on image for an interactive view with Cn3D
Spt4/RpoE2 zinc finger
This family consists of several eukaryotic transcription elongation Spt4 proteins as well as archaebacterial RpoE2. Three transcription-elongation factors Spt4, Spt5, and Spt6 are conserved among eukaryotes and are essential for transcription via the modulation of chromatin structure. Spt4 and Spt5 are tightly associated in a complex, while the physical association of the Spt4-Spt5 complex with Spt6 is considerably weaker. It has been demonstrated that Spt4, Spt5, and Spt6 play roles in transcription elongation in both yeast and humans including a role in activation by Tat. It is known that Spt4, Spt5, and Spt6 are general transcription-elongation factors, controlling transcription both positively and negatively in important regulatory and developmental roles. RpoE2 is one of 13 subunits in the archaeal RNA polymerase. These proteins contain a C4-type zinc finger, and the structure has been solved in. The structure reveals that Spt4-Spt5 binding is governed by an acid-dipole interaction between Spt5 and Spt4, and the complex binds to and travels along the elongating RNA polymerase. The Spt4-Spt5 complex is likely to be an ancient, core component of the transcription elongation machinery.
Statistics
?
PSSM-Id: 399233
Aligned: 36 rows
Threshold Bit Score: 36.1609
Threshold Setting Gi: 74542395
Created: 21-May-2020
Updated: 7-Aug-2020
Structure
?
Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
2EXU_A   4 ERACMLCGIVQTTNEfnrDGCPNCqgifeeagvsTMECTSPSFEGLVGMCKPTKSWVAKWlsVDHSIAGMYAIKVD 79  baker's yeast
O26365   3 EKACTRCKRITSDER-----CPVC-----------NVPASTNWSGLLIIIDPDKSDIARE--LNITLPGEYALRVR 60  Methanothermobacter therm...
Q8PZ97   3 EKVCRHCLRVLEGQT-----CPVC----------GTSDLAEEWNGLVIILDTERSEIAKR--LGVDIPDRFALKVR 61  Methanosarcina mazei
Q9UY22   2 EKACRNCHYITTEDQ-----CPVC----------GSRDLSEEWFDLVIIIDVENSEIAKK--IGAKVPGKYAVRVR 60  Pyrococcus abyssi
O29149   3 ELACKNCRFINVDTN----ICRNC----------GSTDLTKEWYGYVVIVDPEKSEIAKR--LEIKIPGKYALRVG 62  Archaeoglobus fulgidus
Q9HNL2   5 RLACHDCHRIVEPDE---EMCPYC----------SSNSLTEDWAGYVVITHPDTSEIADK--MEVHEAGEFALKVR 65  Halobacterium salinarum
Q8TVE0   4 LKACVRCGYLVEEDT---EICPAC----------HGDEFTENWRGIAVILDTE-SQTADR--LNAKIPGKYALRVE 63  Methanopyrus kandleri
Q975P1   8 FKACKNCRALVPQDT---PKCPVC----------GSISFSDDWSGIVIILDPE-SETAKL--FGATVPWRYAIIVK 67  Sulfurisphaera tokodaii
Q980A2   7 FKACKNCKALVDTDK---ETCPVC----------GSTSFTDEWDGMIIIINSE-SEIAKI--TEAPKPWKYAIIIK 66  Saccharolobus solfataricus
Q97BH1   5 YRACKKCKRLTPEKT-----CPIH----------PDEKTTTEWFGFLIITEPEFSAIAKR--AGISEPGMYAIKVR 63  Thermoplasma volcanium
| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap