Conserved Protein Domain Family
Rep_4

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pfam05797: Rep_4 
Yeast trans-acting factor (REP1/REP2)
This family consists of the yeast trans-acting factor B and C (REP1 and 2) proteins. The yeast plasmid stability system consists of two plasmid-coded proteins, Rep1 and Rep2, and a cis-acting locus, STB. The Rep proteins show both self- and cross-interactions in vivo and in vitro, and bind to the STB DNA with assistance from host factor(s). Within the yeast nucleus, the Rep1 and Rep2 proteins tightly associate with STB-containing plasmids into well organized plasmid foci that form a cohesive unit in partitioning. It is generally accepted that the protein-protein and DNA-protein interactions engendered by the Rep-STB system are central to plasmid partitioning. Point mutations in Rep1 that knock out interaction with Rep2 or with STB simultaneously block the ability of these Rep1 variants to support plasmid stability.
Statistics
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PSSM-Id: 283459
Aligned: 2 rows
Threshold Bit Score: 592.899
Created: 22-Mar-2022
Updated: 17-Oct-2022
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
CAR26932  11 RPDRELRMMNDVLMTYPYTVIHLPAQNMLSTPKGMVNIAENYRDYPILAIFYVKYLMKKSPYQVIPVNLEWPEPYVVLNT 90 
P03871     2 NGERLLACIKQCIMQHFQPMVYDESRCVIETTRGTFPVPDNYKKYKTLAFAFVGHVLNTDDTPVIEKELDWPDPALVYNT 81  Saccharomyces cerev...
CAR26932  91 ILKRLKEH----KF----FANKDKEDFAERLhkliapDVSIPESRKDEILGQQKKERVVTKTISENFLDpvnarPRLQRF 162
P03871    82 IVDRIINHpelsQFisvaFISQLKATIGEGL------DINVKGTLNRRGKGIRRPKGVFFRYMESPFVN-----TKVTAF 150 Saccharomyces cerev...
CAR26932 163 FEKL----------HNGTlvenlevglcKVEILVSSKAMLGQPLKLQFMAANVRELWVGEMVCNMITNETDYGfdeRGGD 232
P03871   151 FSYLrdynkiaseyHNNT----------KFILTFSCQAYWASGPNFSALKNVIRCSIIHEYISKFVEREQDKG---HIGD 217 Saccharomyces cerev...
CAR26932 233 D----DEGSSVEVQNSQ-SASPGQDQEAqrapEAPETSSQLFDKIVSALQDDP-DSAKAQLgqcrkLANYLLSHKREQED 306
P03871   218 QelppEEDPSRELNNVQhEVNSLTEQDA----EADEGLWGEIDSLCEKWQSEAeDQTEAEI-----IADRIIGNSQRMAN 288 Saccharomyces cerev...
CAR26932 307 FFMQTKDTRARLYLDLKGCLGSSWKFSIYRGVRCKQNgQLKVSLK-PSN 354
P03871   289 LKIRRTKFKSVLYHILKELIQSQGTVKVYRGSSFSHD-SIKISLHyEEQ 336 Saccharomyces cerevisiae S288C
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