Conserved Protein Domain Family

pfam05713: MobC 
Bacterial mobilisation protein (MobC)
This family consists of several bacterial MobC-like, mobilisation proteins. MobC proteins belong to the group of relaxases. Together with MobA and MobB they bind to a single cis-active site of a mobilising plasmid, the origin of transfer (oriT) region. The absence of MobC has several different effects on oriT DNA. Site- and strand-specific nicking by MobA protein is severely reduced, accounting for the lower frequency of mobilisation. The localized DNA strand separation required for this nicking is less affected, but becomes more sensitive to the level of active DNA gyrase in the cell. In addition, strand separation is not efficiently extended through the region containing the nick site. These effects suggest a model in which MobC acts as a molecular wedge for the relaxosome-induced melting of oriT DNA. The effect of MobC on strand separation may be partially complemented by the helical distortion induced by supercoiling. However, MobC extends the melted region through the nick site, thus providing the single-stranded substrate required for cleavage by MobA.
PSSM-Id: 399023
Aligned: 30 rows
Threshold Bit Score: 42.0958
Threshold Setting Gi: 380502446
Created: 21-May-2020
Updated: 7-Aug-2020
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
Format: Row Display: Color Bits: Type Selection:
jgi:Cyan7822_6851  59 KLGKISNNINQITKVLNthnkfnanVPYDVIFELKEQLANVSTQIDQI 106 Gloeothece verrucosa PCC 7822
CCG53488           67 ELSRIGNNINQMSKRVN--------QGNPNLKGLESELSSLHKQLNEI 106 Flavobacterium indicum GPTSA100-9 = DSM 17447
CeBiTec:CVAR_2128  81 EIRRIGNNVNQMAKRVNgg----raGALHGLTGVVAELRDQAARLAEI 124 Corynebacterium variabile DSM 44702
CCY58936           61 EVNHIGTNINQIVHNHNsg-----iYSEADKERLVVYMRKLNTEVNNv 103 Clostridium sp. CAG:632
Q8CYS9             63 ELRRIGNNINQIARAINqs----hlISQDQLQELSKGVGELIKEVDKE 106 Streptococcus pneumoniae R6
bgi:05SSU0914      63 ELRRIGNNVNQIARVINqt----hlISMEQLQGLGQGMAELVSEVEKD 106 Streptococcus suis 05ZYH33
CDB88308           55 QQKAIGNNLNQLTVLANmg-----kVQFANLDSAVHEFSKINTALREM 97  Firmicutes bacterium CAG:170
CDB86685           55 QQKAIGNNLNQLAVLANtg-----kVQFANLDAAAQEFAKINVTLRQI 97  Firmicutes bacterium CAG:170
CDE24316           60 QLRKIGNNINQLTKLCNqg-----rLTNINLEDVKKEMKSIWQLLNll 102 Clostridium sp. CAG:440
CDB99542           89 ELRKIGININQIAILCNmg-----kLECVHIEDTKSEIKKVWEELNKL 131 Firmicutes bacterium CAG:41
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