6MSO


Conserved Protein Domain Family
Fumerase

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pfam05681: Fumerase 
Fumarate hydratase (Fumerase)
This family consists of several bacterial fumarate hydratase proteins FumA and FumB. Fumarase, or fumarate hydratase (EC 4.2.1.2), is a component of the citric acid cycle. In facultative anaerobes such as Escherichia coli, fumarase also engages in the reductive pathway from oxaloacetate to succinate during anaerobic growth. Three fumarases, FumA, FumB, and FumC, have been reported in E. coli. fumA and fumB genes are homologous and encode products of identical sizes which form thermolabile dimers of Mr 120,000. FumA and FumB are class I enzymes and are members of the iron-dependent hydrolases, which include aconitase and malate hydratase. The active FumA contains a 4Fe-4S centre, and it can be inactivated upon oxidation to give a 3Fe-4S centre.
Statistics
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PSSM-Id: 399002
Aligned: 375 rows
Threshold Bit Score: 198.401
Threshold Setting Gi: 74509550
Created: 20-May-2020
Updated: 7-Aug-2020
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
6MSO_A            93 TLLSSTAFDDVEHLLRSSHLMSLRKIF---DDpEA---SDNDKFVALQLLKNANISSARLLPGCQDTGTAIIAGYRGDQV 166
jgi:Spica_1190    41 TKLAEEAFVRISYYLRPSHLKLWSEQL---NSnTL---TANDRLVLETLLKNALISAEGKLPLCQDTGTATIIGWKEESV 114 Treponema c...
jgi:Desku_3104     8 siAARDALIQASTTFRPDQVEAYQQAL---ER-ET---SPRGRWVLEQILKNAKVAREKKYPLCDDTGIPHVVLEIGDQV 80  Desulfofund...
WP_014547259       5 EQKVCECLIQAGSTFREDQKEAYRRAI---AK-EP---GTNSCWVMQQVLDNALVAEKNRGPLCDDTGIPHIFIEVGPNR 77  Fibrobacter...
CCY70083           7 vTKVCDTLVKAGSTFLGDKKEAYKKAI---AK-EA---NEQAKWTMEKILENAYVAEKNQSPLCDDTGIPHLVLEVGPGK 79  Eubacterium...
agres:mru_0690     4 IKKVEDAVVKAGSGYSEDRLTAYENAL---RL-EEekgNENAVWALEQMIENFKVAKENKLPLCDDTGIPHIIIEIGYNR 79  Methanobrev...
NZAGRC:Abm4_0418   4 INKIADGVIKASTTYSQDRIDGFKRAL---KL-EEelsNDNAVWALEQMIRNESIASLRKLPLCDDTGIPHILVELGENR 79  Methanobrev...
WP_023992715       6 RDLVKDAVITASTTFREDQIQAYQQAI---QK-EE---NDNARWVLKLLLENARIAQSKKVPLCDDTGIPHVLVELGPET 78  Methanobact...
WP_013413748       4 VKKVKKAVIKCSTEYSEDQIKAYKNAI---KN-EN---NENAVWVLKLLLKNAKIAKKKRRPLCDDTGIPHVYIEIGKKS 76  Methanother...
WP_015711790      43 SQAAEEGFRRLSFFFRESHLAMLAEQLalaENdKK---PGNDGFVLNALLRNAVIAAKGELALCQDTGTAVIYGWKSENV 119 Treponema a...
6MSO_A           167 FvpGn--D-EEALSRGVYDIFQKRNFRYSQNVPLsmY-DEKNTGT---NLPAQIDLYASKg-----ME------------ 222
jgi:Spica_1190   115 R--TgaeDgEA-LSNGVLQAYTGHPLRASQVGFRs-LfDEFDTGN---NLPAQIHIEAVRdns-egPE------------ 174 Treponema c...
jgi:Desku_3104    81 Ri-Sg--KvFQAVAEGIREGLRALPGRPMAVRGEg-M-ERLGQVLgmyDDPGMLEAA--PvtvysiSGn----------- 142 Desulfofund...
WP_014547259      78 Qv-Tg--DvLAQIHAGIEAGLRKLPGRPMAILGDd-Y-HRIDQSGglnPDSGALAPS--PilikpvEEd----------- 139 Fibrobacter...
CCY70083          80 Ai-Tg--EmIESIKEGVRCGLRKLPGRPMAIKGNd-S-QRIDQSQgleDDPGMLEAA--PilirniKEd----------- 141 Eubacterium...
agres:mru_0690    80 Ei-Pk--NfFNDINAGIANGLDKLPGRPMAVKGNd-I-ERIEQSKglyAESNMMKPA--Sfl--ieEKddstygrkigid 150 Methanobrev...
NZAGRC:Abm4_0418  80 Sl-Pe--GfIRDIKEGLKQGLDDLPGRPMAVKGNp-L-ERIEQSKglyDEPSMMLPA--PisidsfDKnnrsys---rdl 149 Methanobrev...
WP_023992715      79 Sf-Kp--DlFHQIEEGVADGLRKLPGRPMAVCGDd-I-ERVEQSRglsSDPGKVISP--SilvdkmDGn----------- 140 Methanobact...
WP_013413748      77 Ei-Ss--TfFQKIRRGIAEGLRELPGRPMALKGNd-I-ERIEQSKgiyEDPGKVVPP--SffidnnKEen---------- 139 Methanother...
WP_015711790     120 F--TgsgDdDAWLESGIGAAYKKNNLRLSQIGASs-FlDEFNTGN---NLPAQIQIHASGk----nPV------------ 177 Treponema a...
6MSO_A           223 ---YSFMFVAKGGGSANKSFLlqetkS-----VL--------NPKSLRNFLKEKLAMFGTSACPPYHVAVVIGGTSAEMT 286
jgi:Spica_1190   175 ---YRFLFIAKGGGSANKTAL-----FqmtkaLL--------EPETFKAFLKEKIAALGTAACPPYRLAVVVGGTSPEEN 238 Treponema c...
jgi:Desku_3104   143 --iLKITVLMLGGGPEIRGKT-----M-----RVfhrhrgenViEEVGRWAAEASAALG---CTPCVPIVGIGRTQYEAA 207 Desulfofund...
WP_014547259     140 --vLRLTILMLGGGPAIRGIT-----Q-----RVfhkhsvqvViDEIVARAIPAVSQLG---CSPCTLAVGIGRSQFEAT 204 Fibrobacter...
CCY70083         142 --kIKLNILMFGGGPAIRGRT-----Y-----RVfhkhdtnvVlDEIVSWAKEATKQLG---CTPCTLGVGVGRSNYEAA 206 Eubacterium...
agres:mru_0690   151 ddkIRINVLLEGGGPEIRAKT-----Y-----RVfhkrdsniVfNEALSWLKESLSMLG---CTPSIPAIGIGRSHFEAN 217 Methanobrev...
NZAGRC:Abm4_0418 150 enqIHIHILLLGGGPEIRSKT-----Y-----RVyhqhdrkiViNETIDWLKESLKMLG---CTPSIPAIGIGRSHYEAS 216 Methanobrev...
WP_023992715     141 --gLKIHVLMLGGGPEIRSHT-----Y-----RVfhqrdhgnLfKEALTWMRSEIPKLG---CTPCIPALGIGRTHFEAS 205 Methanobact...
WP_013413748     140 --gTKIHIMMLGGGPEIRART-----K-----KVfhehdyrkVfKEVIRWIIVEAKSLG---CTPCVPAIGIGRTHYEAT 204 Methanother...
WP_015711790     178 ---YRFLFIAKGGGSSNKTSL-----FsmtksLL--------EEQAFEKFLEEKIKALGTAACPPYRLAVVVGGTSPEFN 241 Treponema a...
6MSO_A           287 MKVLKyASchyYDDLI--TKP-DMKTgyt----frdLELEEEVLKVCQNIGMGAQ-FGGKYYAHDVRVIRMPRHGASCP- 357
jgi:Spica_1190   239 LSILKlATtelLDTAPrmESAfKNNGshwl---yrdTEWESILMNITRESGLGAQ-FGGSLLALDARLIRLPRHAGSCP- 313 Treponema c...
jgi:Desku_3104   208 ALQLA-AA----------LEGdFTRQ----------SPWEAKITEMVNNTGVGPLgLGRSVTALGSFVKIGPARASGVRi 266 Desulfofund...
WP_014547259     205 SMMME-AM----------AKGnYDFQ----------SEFEKTITERVNESNVGALgLGGKTSVLATFAKVGPQRASGVRi 263 Fibrobacter...
CCY70083         207 SMMLE-AL----------VDGtYDRQ----------SPMEKYITEKVNEERTGPLgLGGDTTVLATFMKVGPQRASGVRv 265 Eubacterium...
agres:mru_0690   218 SLMLR-AM----------VHGnLNDQ----------SDIEKYIADELNKTNIGPLgLGGVTTVLGSFVNIGYQRASGVRi 276 Methanobrev...
NZAGRC:Abm4_0418 217 HLMLK-AM----------VYGnLNYQ----------SNTENFITNELNKLNIGPMgLKGHTTVLGTFLNIGPQRASGVRi 275 Methanobrev...
WP_023992715     206 SQMLK-AM----------VYSnLNHQ----------SSIEKKITDSLNRTNVGALgLGGSVTALGSMIKIGPQRASGVRi 264 Methanobact...
WP_013413748     205 SLMLK-AI----------AHGkLDQQ----------SELEEYITNSVNSSGIGPLgLGGSTTALGTLIKIGPQRASGVRi 263 Methanother...
WP_015711790     242 LEACKlASaeaLDSAPyfEPGeDKEKsaslpwirrdKQWEEKAMSIGRRTGLGAQ-FGGSSLLLDARVLRLPRHAASCP- 319 Treponema a...
6MSO_A           358 IGIGVSC-SADRQA 370
jgi:Spica_1190   314 VSIGVSC-SAHRNM 326 Treponema caldarium DSM 7334
jgi:Desku_3104   267 VSLRLGCcFDPRRA 280 Desulfofundulus kuznetsovii DSM 6115
WP_014547259     264 VALRPCCcFEPRRA 277 Fibrobacter succinogenes
CCY70083         266 VCLRPCCcFEPRIA 279 Eubacterium sp. CAG:161
agres:mru_0690   277 VAVRPACfVEPRVs 290 Methanobrevibacter ruminantium M1
NZAGRC:Abm4_0418 276 VCARPCCfVEPRIS 289 Methanobrevibacter sp. AbM4
WP_023992715     265 VCMRPCCcVEPRKA 278 Methanobacterium sp. MB1
WP_013413748     264 VSTRLCCcVEPRKY 277 Methanothermus fervidus
WP_015711790     320 VSIGVSC-AAHRNL 332 Treponema azotonutricium
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