Conserved Protein Domain Family
DUF608

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pfam04685: DUF608 
Glycosyl-hydrolase family 116, catalytic region
This represents a family of archaeal, bacterial and eukaryotic glycosyl hydrolases, that belong to superfamily GH116. The primary catabolic pathway for glucosylceramide is catalysis by the lysosomal enzyme glucocerebrosidase. In higher eukaryotes, glucosylceramide is the precursor of glycosphingolipids, a complex group of ubiquitous membrane lipids. Mutations in the human protein cause motor-neurone defects in hereditary spastic paraplegia. The catalytic nucleophile, identified in UniProtKB:Q97YG8_SULSO, is a glutamine-335, with the likely acid/base at Asp-442 and the aspartates at Asp-406 and Asp-458 residues also playing a role in the catalysis of glucosides and xylosides that are beta-bound to hydrophobic groups. The family is defined as GH116, which presently includes enzymes with beta-glucosidase, EC:3.2.1.21, beta-xylosidase, EC:3.2.1.37, and glucocerebrosidase EC:3.2.1.45 activity.
Statistics
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PSSM-Id: 398386
Aligned: 35 rows
Threshold Bit Score: 289.526
Threshold Setting Gi: 81443807
Created: 20-May-2020
Updated: 7-Aug-2020
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Q7KT91   505 GRFGYLEG-HEYRMYNTYDV-H-------FYA-SPALAHLWPNLQVSLQYDF--------KDAIAAELNDTRKMLY---- 562  fruit fly
Q1IR11   436 DSFSYLEC-FDYAYYGTLDV-R-------FYG-SFPLAKFWPEIEKQEMREY--------ADTIPENIDQKYIWAW---- 493  Candidatus Koribac...
Q7NHS5   487 GRFAVLES-IDYRWYDSLDV-R-------LYG-SFALLINWPEIDKAVLRSY--------ADAVPDEDDSTRIIGY---- 544  Gloeobacter violaceus
Q8DMC7   429 GQFAVLEC-LDYRWYESLDV-R-------LYG-SFALLQLWPELEKSVMRAF--------ARAIPTADPTLRIIGYfyrg 490  Synechococcus elon...
P73619   418 GQFGVCEC-MDYRWYESLDV-R-------LYG-SFGLLMHWPKLEQAVMLAF--------ARAIPTEDPKERVIGY---- 475  Synechocystis sp. ...
Q6EUT3   485 GRFGYLES-WEYRMVNTYDV-H-------FYA-SFALAELWPELEITIQSEF--------TDQVYHSIEKPTRFHM---- 542  Caenorhabditis ele...
CAP29853 446 GRFGYMES-WEYFMINTYDV-H-------FYS-SWAILKNWPQIEMSMQMDF--------ADQVDRVDYGTATSLA---- 503  Caenorhabditis bri...
PDM73688 444 GRFGYMES-WEYLMYNTYDV-H-------FYA-SAAFIENWPQLEVSMQLDF--------SEQLNREDTRLHKTLM---- 501  Pristionchus pacif...
Q8A3P6   409 GRLWTWEG-----CADNWGScHgscthvwNYA-Q-AIPHLFPSLERSLRHTEfeegqdlkGHQVFRVN------------ 469  Bacteroides thetai...
Q97VF2   305 GRFAVYEDpFVTKLMNTIGSmT-------FDGlGFTLLELYRDLVISADNYF---------------------------- 349  Saccharolobus solf...
Q7KT91   563 ---DGKV------MPRKVKNCVPHDLG-DPDE---------------EPFTLINCYNIHDVNDWKDLNTKFVLQVYRDYY 617  fruit fly
Q1IR11   494 ---KSDNehklepTQRKVAGSAPHDLG-SPLE---------------DALILPNQYNYQSVSFWKDLNSKYVLMVWRDYV 554  Candidatus Koribac...
Q7NHS5   545 ---TKTV------AKRKSRDALPHDLG-APNE---------------DPWIKNNYTTYQDCNLWKDLPCDFVLQVYRDYV 599  Gloeobacter violaceus
Q8DMC7   491 dpeTAYK------APRKLANAVPHDLG-APNE---------------HPWEKTNYTAYQDCNLWKDLASDFVLLVYRDFL 548  Synechococcus elon...
P73619   476 ---NLSL------APRKVKNATPHDLG-APNE---------------HPWERSNYTAYQDCNLWKDLGSDFVLLVYRDFL 530  Synechocystis sp. ...
Q6EUT3   543 ---EGDW------ANVKTASRVPHDLG-NPAD---------------EPWIATNAYVMHDTGKWKDLNMKYVLTSWRDYV 597  Caenorhabditis ele...
CAP29853 504 ---DGDT------MTIKSYDRIPHDMG-HPSNphlpsiqehhrlsvaDPWIHTNAYILHDTGHWKDLNLKFVISCYRDWK 573  Caenorhabditis bri...
PDM73688 502 ---DGET------VIVKKRGRLPHDMG-HPID---------------SPWEYTNTYCLHDTCEWKDLNLKFILASLRDYQ 556  Pristionchus pacif...
Q8A3P6   470 -------------LPIRPTRHNFHSAA--------------------------------------DGQLGGIMKVYREWR 498  Bacteroides thetai...
Q97VF2   350 -------------MNYVNYGETPHDVGeESIE---------------DPIYGASY-----PYWWTDLGPTLVLMLYRDYV 396  Saccharolobus solf...
Q7KT91   618 VL-NElaqaqsdnaskfssiefidkeslyelysqdnkrknsadekqqnrksasmyinetngkvylmdaIGYLKAMYASCK 696  fruit fly
Q1IR11   555 MSgSKd--------------------------------------------------------------KAFLQYNYNAVK 572  Candidatus Koribac...
Q7NHS5   600 DTgKRd--------------------------------------------------------------LGFVRYCWPASK 617  Gloeobacter violaceus
Q8DMC7   549 FTgGTd--------------------------------------------------------------LNFARECWPAVV 566  Synechococcus elon...
P73619   531 LLpDAd--------------------------------------------------------------GEFLGECWPSIV 548  Synechocystis sp. ...
Q6EUT3   598 VLsNEh--------------------------------------------------------------EDFLFHTWPAVR 615  Caenorhabditis ele...
CAP29853 574 MIaEGee-----------------------------------------------------------dsQEILEFFLGKCT 594  Caenorhabditis bri...
PDM73688 557 QSiKTenp----------------------------------------------------------kaTEVLAHLFERSM 578  Pristionchus pacif...
Q8A3P6   499 ISgEN----------------------------------------------------------------EFLISMYPKVK 514  Bacteroides thetai...
Q97VF2   397 FT-SN---------------------------------------------------------------REILEKNYNKIK 412  Saccharolobus solf...
Q7KT91   697 AIMERTIE-YDKDNDGLIENTKMPDQTYDS--WVMDGPSAYCSGLWLAALQAMSAMATIL-------------DQPNDCL 760  fruit fly
Q1IR11   573 QSMQFLRQ-FDKNNDGLIENDGYPDQTYDN--WSARGESAYSGSLYLAALRATEEMAKLL-------------GDQRTAA 636  Candidatus Koribac...
Q7NHS5   618 AALVRLKTtFDQDGDGFPENGGPPDCTYDA--WPLKGISAYCGGLWLAALLAAVEMGKLV-------------GDAAAVQ 682  Gloeobacter violaceus
Q8DMC7   567 AALDHLKQ-FDQDGDGLPENGGAPDQTYDD--WKLQGVSAYCGGLWLAALEAAIALGTLL-------------Q-QPQVE 629  Synechococcus elon...
P73619   549 AALDYLKG-FDLDNDGIPENSGAPDQTFDD--WRLQGISAYCGGLWIAALEAALVMGQYLlenppqqtdldamEIQTAIA 625  Synechocystis sp. ...
Q6EUT3   616 MIMLEALEnWDQDGDGMIENFGKADQTYDA--WQMEGVSAYCGSLWLASLRVAIEMAGLM-------------KDDETQQ 680  Caenorhabditis ele...
CAP29853 595 KIVDGALEnWDKDKDGMIENDGFADQTYDV--WKMTGTSAYCGSLWIAALTSYIQMLKRA-------------GIPTKD- 658  Caenorhabditis bri...
PDM73688 579 EIIEAGKKeWDVDGDGMIENGGFADQTYDV--WIMTGTSSYCGSLWIAALEAARRMAEIL-------------GRKTEEA 643  Pristionchus pacif...
Q8A3P6   515 KSLDYCIStWDPRRVGSIEEP--HHNTYDIefW---GPDGMHNSFYYGALSAFIRMSEFL---------------DKDVT 574  Bacteroides thetai...
Q97VF2   413 EIIDWLIR-KDMDNDCIPDSKGGYDNSYDG--THMYGASSYIASMFLSALTAFIKMSEIL-------------DVKIDDK 476  Saccharolobus solf...
Q7KT91   761 RYQDILEKGKRSLEEKLWNGSYY-------------------------------------RFDLSHSHRDTIMADQLCGH 803  fruit fly
Q1IR11   637 DTAALFKRAQDSFVKKLWNGTYF-------------------------------------NYDVGSSYKDAIMAEQLAGQ 679  Candidatus Koribac...
Q7NHS5   683 QFGAWYAQAQPLYEKTLWNGRYY-------------------------------------RIDSQSKNSEAIMADQLCGE 725  Gloeobacter violaceus
Q8DMC7   630 IYRQWLSQARPRYHQLLWNGEYY-------------------------------------RLDTGS-GSDVIMADQLCGQ 671  Synechococcus elon...
P73619   626 RYETWLAQARSLYQDTLWNGEYY-------------------------------------NLDSQS-GSKVVMADQLCGQ 667  Synechocystis sp. ...
Q6EUT3   681 LFRNTLEKAKKVFIDTLWTGTYF-------------------------------------RFCERSRSRETVMADQLCGY 723  Caenorhabditis ele...
CAP29853 659 -YEEKLLMAYEAYTTKLWNGKFF-------------------------------------KFDELPDNSKIVMADQLCGF 700  Caenorhabditis bri...
PDM73688 644 SLAQQVDKARSIFIKKLWNGRYF-------------------------------------NFDE---TSSSIMADQLCGV 683  Pristionchus pacif...
Q8A3P6   575 EYKKLLKKGRKFTETGLFNGEYFiqkiewrglnakdptvaqsfhssyspeakeilekegpKYQ----YGNGCLSDGVLGS 650  Bacteroides thetai...
Q97VF2   477 -YYRFLECGKKTFN-SLWNGKYFil---------------------------------wkKNDE---ENTSCLNSQLLGQ 518  Saccharolobus solf...
Q7KT91   804 WYLKSCGFDYe----IYPKENVRTALKRIYDNNVMG------------F----HEGNIGAANGFI-ANaseptkpGHVDN 862  fruit fly
Q1IR11   680 WYASLTGLGD-----LVPRDMQHSALKKVYDFNVMK------------L----QNGTMGALNGIS-AS-------GEVLK 730  Candidatus Koribac...
Q7NHS5   726 YYAQVCNLAD-----IVPEPQARSALETVYQTCFVK------------F----YGGRFGCANGTN-AD-------GSFIG 776  Gloeobacter violaceus
Q8DMC7   672 FYAQLLGLVD-----IVPPDCCDRALRKIYDTCFLK------------F----HNGQFGAANGLL-PN-------GQPEN 722  Synechococcus elon...
P73619   668 FYARLLNLPD-----VVEHHYAEKALAKVYDTCFLK------------F----AHGELGAANGLL-PD-------GSPQN 718  Synechocystis sp. ...
Q6EUT3   724 WFLQS--VSPeladeLLPNHMVRSALDTIYRLNVCK------------F----GNGRMGAVNGMK-PS-------GVVDR 777  Caenorhabditis ele...
CAP29853 701 WALTAMDEPI-----QVSEGKIQSALETIFKYNVEM------------Y----DGGKCGAVNGFL-AS-------ERVDG 751  Caenorhabditis bri...
PDM73688 684 WFMAALDGTTdk--mLIDKEKVSSVLDTIFANNVKK------------FgskeSAGKSGAANGWK-ED-------SGLDT 741  Pristionchus pacif...
Q8A3P6   651 WLSRMCGMEE-----TLNTEKVKSHLLSVHRYNFKKdltdhanpqrspY----ALGKEGGLLLGSwPK-------GGKLS 714  Bacteroides thetai...
Q97VF2   519 FWCDILGLPP-----ITDHDKINTALRSIYELN---------------F----KASKYCLTNAVR-ED-------GSVDS 566  Saccharolobus solf...
Q7KT91   863 -SNIQAEEVWPGVVYALAATMIQ------EGMFEEAFQTAGGMYKTLSQRIGMNFETP---EALYGE--KRYRSI-GYMR 929  fruit fly
Q1IR11   731 -DNEQTEEVWTGVTFAVAATMLQ------NGLREEGFNTAKGVYNVVYDQKGYWFRTP---EAYDTN--GMYRAS-MYMR 797  Candidatus Koribac...
Q7NHS5   777 -DTEHPSEVWTGINFGLAAFMIR------NGMRREGMAIAEAVVANVYGG-GLQFRTP---EALTPA--RTFRAC-MYLR 842  Gloeobacter violaceus
Q8DMC7   723 pHATHPLEVWTGINFGLAAFLWQ------RGMIDEAWRLAEVVVRQIYEN-GLQFRTP---EAITAN--GTFRAC-MYLR 789  Synechococcus elon...
P73619   719 pNDTHPLEVWTGINFGLAAFLIQ------MGMKEEALKMTETVVNQVYNN-GLQFRTP---EAITAV--GTFRAS-HYLR 785  Synechocystis sp. ...
Q6EUT3   778 -EYIQADEMWTGVTYAVASLLIQ------QGEVEKAFHTASGSYLTCFEETGLQYQTP---EALYES--KFYRAI-GYMR 844  Caenorhabditis ele...
CAP29853 752 -SSIQSEEVWAGITYSLSAMMIEkapiylQGMDEMAFKTSEGLFHSIWNRFPLQYQTP---EAITAD--GMYRAL-GYMR 824  Caenorhabditis bri...
PDM73688 742 -TAIQSEEMWTGTTYALASFFAL------MGDRERCFAVAEGVYDSCWKRAGLQYQTP---EAMFQW--MFYRAI-GYMR 808  Pristionchus pacif...
Q8A3P6   715 lPFVYSNEVWTGIEYQVASHLML------QGEVEKGLEIVRACRQR----YDGSVRNPf----------NEYECGhWYGR 774  Bacteroides thetai...
Q97VF2   567 -STAQLRSCWPRVSFAVAAHMIL------RGMVKEGIEVAKREWETIKEL------NPfdqSSRIDAidGKYVGLmSYIG 633  Saccharolobus solf...
Q7KT91   930 PLSIWSM 936  fruit fly
Q1IR11   798 PGAIWSM 804  Candidatus Koribacter versatilis Ellin345
Q7NHS5   843 PMAIWAM 849  Gloeobacter violaceus
Q8DMC7   790 PMAIWAL 796  Synechococcus elongatus
P73619   786 AMAIWAV 792  Synechocystis sp. PCC 6803
Q6EUT3   845 PLSIWAM 851  Caenorhabditis elegans
CAP29853 825 PLSIWAI 831  Caenorhabditis briggsae
PDM73688 809 PLAIWNI 815  Pristionchus pacificus
Q8A3P6   775 ALSSYGL 781  Bacteroides thetaiotaomicron
Q97VF2   634 STSVWLL 640  Saccharolobus solfataricus
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