2NYS


Conserved Protein Domain Family
SspB

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pfam04386: SspB 
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Stringent starvation protein B
Escherichia coli stringent starvation protein B (SspB), is thought to enhance the specificity of degradation of tmRNA-tagged proteins by the ClpXP protease. The tmRNA tag, also known as ssrA, is an 11-aa peptide added to the C-terminus of proteins stalled during translation, targets proteins for degradation by ClpXP and ClpAP. SspB a cytoplasmic protein that specifically binds to residues 1-4 and 7 of the tag. Binding of SspB enhances degradation of tagged proteins by ClpX, and masks sequence elements important for ClpA interactions, inhibiting degradation by ClpA. However, more recent work has cast doubt on the importance of SspB in wild-type cells. SspB is encoded in an operon whose synthesis is stimulated by carbon, amino acid, and phosphate starvation. SspB may play a special role during nutrient stress, for example by ensuring rapid degradation of the products of stalled translation, without causing a global increase in degradation of all ClpXP substrates.
Statistics
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PSSM-Id: 398197
Aligned: 126 rows
Threshold Bit Score: 107.233
Threshold Setting Gi: 119374411
Created: 11-May-2020
Updated: 7-Aug-2020
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
2NYS_B                           10 ILAQDALRGVIRKVLGeva-atGrLPgdhhfFITFLTGAPGVRISqhlkSKYAEQXTIVIQHQFWDXKVTET--GFEIGL 86  Agrobacterium fabrum str. C58...
jgi:Pden_1909                     9 GMMHRAMQRLIAEVLEtva-eeG-LPgehhfFITFDTRDSDVEMAdwlrERYPEEMTIVIQHWFENLSVTPE--GFHITL 84  Paracoccus denitrificans PD1222...
PRJNA212980:JCM7686_0863          9 GMMHRAMQGLIADVLRsva-aeG-LPgehhfFITFDTRDPDVQMAdwlrERYPEEMTIVIQHWFDNLVIEKD--HFEITL 84  Paracoccus aminophilus JCM 7686...
jgi:Astex_1747                   15 TLTQNALRGVIREALIkashphG-LPgdhhfYVTFLTRAEGVTIPddllKRYPRDITIVLQHQYRELKVDGD--RISVTL 91  Asticcacaulis excentricus CB 48...
dbos:1099484000104_PB2503_09039  12 FLELAALRNVVRDVLSite-tlGhPPgehhfYIGFLTNAKGVEMSdtlrEQYPERMVIVLQHQFEGLRVYAD--RFEVTL 88  Parvularcula bermudensis HTCC2503...
WP_003624616                     24 RWLNEASRSVMLNALEhvs-reG-LAgehhfYLTFLTDFPGVDIParlrAQYPHEMTIVLQHQFWDLKVDRKakTVSVGL 101 Acetobacter pasteurianus...
Q5FR19                           25 DWVEDAYREVMLRAIEhva-aeG-LPgahhfYLTFRTNRPDVIIParlrAQYPEEMTIVLQHQFWDLALDREknVISVGL 102 Gluconobacter oxydans...
WP_012223651                     24 SWIEDAYRTVMLRALElvg-reG-LPeghhfYITYLTNWPGVELParlrAQYPHEITIVLQHQFWDLKVDEAsrTVSVGL 101 Gluconacetobacter diazotrophicus...
WP_003617242                     24 DWIEDAYRDVMLRAFEyvvtegG-LPgdhhfYLTFRTDWPGVEMPervrAQYPHEITIVLQHQFHGLKVDRAagTISVSL 102 Komagataeibacter hansenii...
Q0BR46                           51 EWTEQALRQVVARALEhva-ndG-MPeghhfYITFRTDYFGVSIPsrlkAQYPHEMTIVLQHQFWDLAVGPD--SFSVGL 126 Granulibacter bethesdensis CGDNIH1...
2NYS_B                           87 SFSDTPEKLVIPYNAIRGFYDPSVNFELEFdVPLakeeexeeae------------------itaypvsheaKPASETPK 148 Agrobacterium fabrum str. C58...
jgi:Pden_1909                    85 NFGNSPEPLYIPFDALRTFVDPSVEFGLRFeNHEede---------------------------------dePEAEEDEP 131 Paracoccus denitrificans PD1222...
PRJNA212980:JCM7686_0863         85 NFGNSPEPLHVPFDAVRTFVDPSVEFGLRFeTQNedd---------------------------------deEDDEEDED 131 Paracoccus aminophilus JCM 7686...
jgi:Astex_1747                   92 SFGGVPKVLRFPLSAITRFYDPSVQFILEFdVEEpleandde-----------------------gliaepaAATDEGKP 148 Asticcacaulis excentricus CB 48...
dbos:1099484000104_PB2503_09039  89 HFKGVPDRLIIPFDAIAEFADPSVNYSRQFpVAIevlgetdtaaptvqgsv-----epftprgdksrpahrdDNDDDGPD 163 Parvularcula bermudensis HTCC2503...
WP_003624616                    102 SFGGVGSILVIPVQAITGFADPAIRFSLHFaAMEpteeptp------------------------eepttgqPEQDGPEE 157 Acetobacter pasteurianus...
Q5FR19                          103 SFGGVGSILCIPVNAITAFADPHIRMALRFsHSPleededveipe-----------------gavepddidpAHHPEVQP 165 Gluconobacter oxydans...
WP_012223651                    102 SFGGVGSTLVIPIDAITAFADPHIRLALRFtPPElased----------------------------eepdgAEAPDAAP 153 Gluconacetobacter diazotrophicus...
WP_003617242                    103 AFGGVPATLVIPVAAISAFADPSVKLPLSFrVPEhppqpapsaeihnvfg-------qgqgqgprapqpeqeAHAPGAQD 175 Komagataeibacter hansenii...
Q0BR46                          127 SFGGVASTLRIPFAALLGFADPHVGVGLRFqADEpgdenasederddeaedgsesgklatfpgsaaasstasPATAEGEQ 206 Granulibacter bethesdensis CGDNIH1...
2NYS_B                          149 SGEEKK-----------EGSVV 159 Agrobacterium fabrum str. C58
jgi:Pden_1909                   132 QEPGEDgp-------kgSAEVV 146 Paracoccus denitrificans PD1222
PRJNA212980:JCM7686_0863        132 DAEDGVevaeaeeapktGAEVV 153 Paracoccus aminophilus JCM 7686
jgi:Astex_1747                  149 ADPDTPpp-------dtGPKVV 163 Asticcacaulis excentricus CB 48
dbos:1099484000104_PB2503_09039 164 DDGPSGpq-------sgSADIV 178 Parvularcula bermudensis HTCC2503
WP_003624616                    158 SDSSSQvvsldafrkkgpspa- 178 Acetobacter pasteurianus
Q5FR19                          166 ETTSSEdv-------kkDAEVV 180 Gluconobacter oxydans
WP_012223651                    154 ADTEARae-------esSSQVV 168 Gluconacetobacter diazotrophicus
WP_003617242                    176 ATPTSAeet-----qpsSPQVV 192 Komagataeibacter hansenii
Q0BR46                          207 ENEDSAsa-------qsAPQVV 221 Granulibacter bethesdensis CGDNIH1
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