Conserved Protein Domain Family
FlgM

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pfam04316: FlgM 
Anti-sigma-28 factor, FlgM
FlgM binds and inhibits the activity of the transcription factor sigma 28. Inhibition of sigma 28 prevents the expression of genes from flagellar transcriptional class 3, which include genes for the filament and chemotaxis. Correctly assembled basal body-hook structures export FlgM, relieving inhibition of sigma 28 and allowing expression of class 3 genes. NMR studies show that free FlgM is mostly unfolded, which may facilitate its export. The C terminal half of FlgM adopts a tertiary structure when it binds to sigma 28. All mutations in FlgM that prevent sigma 28 inhibition affect the C-terminal domain and is the region thought to constitute the binding domain. A minimal binding domain has been identified between Glu 64 and Arg 88 in Salmonella typhimurium. The N-terminal portion remains unstructured and may be necessary for recognition by the export machinery.
Statistics
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PSSM-Id: 398146
Aligned: 270 rows
Threshold Bit Score: 29.7705
Threshold Setting Gi: 315180471
Created: 20-May-2020
Updated: 7-Aug-2020
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
PRJNA194094:J421_0166  46 SVQISDAGRA-LSTGE----TSgsssLTPERVAELRRRVLEGAYnaNHVVSQVAQRIL 98  Gemmatirosa kalamazoonesis
WP_013841255           39 SLQLSQSAKYmQEIECalrdLPe---IREELVNSIKTGLRTGTY--QPDAKKIADGIM 91  Desulfotomaculum ruminis
WP_011878746           36 SVQLSHEAKFkKEIENtlkeLPe---VREDIVNKLKKEIQAGTY--KPDAHKIADGIL 88  Desulfotomaculum reducens
goetting:EAL2_c03140   33 KIEISQAGREyQIAMEaarkLPd---IRQEKVEAIRLELESGTY--KVDADKIARGIL 85  Peptoclostridium acidaminoph...
jgi:Hprae_1880         32 STQISAKAKEiMTARTelkkVPq---IREEKVAALKKEVQAGNY--QIDSKTLATKII 84  Halanaerobium praevalens DSM...
WP_014966456           35 QVNISNMARDcQFAKNevkkLPd---IRTEKVEEIKRQIQTGTY--EINADKIAEKIV 87  Gottschalkia acidurici
WP_012576076           32 QVEISSAAKElQEAAKf---SSe----RLEKVERLKSEVEAGTY--TIDARAIAESVL 80  Anoxybacillus flavithermus
WP_013913916           35 QVEISSQAKElLESQSvksaGAaa-dARAEKIQSLKQAVQSGTY--SVDAGKLAERLL 89  Paenibacillus mucilaginosus
WP_025336801           35 EVTISTEAMKlLEAQN----SGkadnERAQRIQSLKQQVSAGTY--QVDASKLAEKLA 86  Paenibacillus sabinae
WP_013781943           36 GMTLSDEARLfKDIAIaakaAPd---IRQDKVESIKNAINSGLY--NIDAGKIAEGII 88  Mahella australiensis
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